(Circular) (Six-base) MAPSORT of: pFN21AE6758.seq Check: 4157 from: 1 to: 6454 pFN21AE6758 6454 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4264 278 Size: 53 83 186 936 2728 2468 Fragments arranged by size: 2728 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2525 3261 3797 1937 Size: 588 736 536 4594 Fragments arranged by size: 4594 736 588 536 Acc65I G'GTAC_C Cuts at: 3775 3775 Size: 6454 AclI AA'CG_TT Cuts at: 1563 4581 4954 1563 Size: 3018 373 3063 Fragments arranged by size: 3063 3018 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2102 2454 3017 3097 3288 3692 4517 5901 Size: 470 352 563 80 191 404 825 1384 Cuts at: 5901 6169 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 825 563 470 404 352 268 191 80 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6257 AflIII A'CryG_T Cuts at: 1203 2306 5353 6330 1203 Size: 1103 3047 977 1327 Fragments arranged by size: 3047 1327 1103 977 AgeI A'CCGG_T Cuts at: 5285 5285 Size: 6454 AhdI GACnn_n'nnGTC Cuts at: 5183 5183 Size: 6454 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6454 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6454 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3113 3145 3113 Size: 32 6422 AlwNI CAG_nnn'CTG Cuts at: 3508 3571 4197 5769 3508 Size: 63 626 1572 4193 Fragments arranged by size: 4193 1572 626 63 ApaLI G'TGCA_C Cuts at: 4511 5667 4511 Size: 1156 5298 ApoI r'AATT_y Cuts at: 1493 2279 2569 3616 3763 3989 4141 6250 1493 Size: 786 290 1047 147 226 152 2109 1697 Fragments arranged by size: 2109 1697 1047 786 290 226 152 147 AseI AT'TA_AT Cuts at: 160 1032 5008 160 Size: 872 3976 1606 Fragments arranged by size: 3976 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6454 AvaI C'yCGr_G Cuts at: 1870 1943 3779 1870 Size: 73 1836 4545 Fragments arranged by size: 4545 1836 73 AvrII C'CTAG_G Cuts at: 2812 2812 Size: 6454 BamHI G'GATC_C Cuts at: 2773 3784 4151 2773 Size: 1011 367 5076 Fragments arranged by size: 5076 1011 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3379 3775 5230 618 Size: 325 217 627 1592 396 1455 1842 Fragments arranged by size: 1842 1592 1455 627 396 325 217 BanII G_rGCy'C Cuts at: 1391 2339 3773 1391 Size: 948 1434 4072 Fragments arranged by size: 4072 1434 948 BbeI G_GCGC'C Cuts at: 3383 3383 Size: 6454 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2692 928 Size: 971 793 4690 Fragments arranged by size: 4690 971 793 BbvCI CC'TCA_GC Cuts at: 3375 3375 Size: 6454 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3457 3491 4645 4679 1903 Size: 34 1520 34 1154 34 3678 Fragments arranged by size: 3678 1520 1154 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3262 4346 5556 3262 Size: 1084 1210 4160 Fragments arranged by size: 4160 1210 1084 BclI T'GATC_A Cuts at: 1548 1548 Size: 6454 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5065 136 Size: 107 122 71 631 605 3393 1525 Fragments arranged by size: 3393 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6449 6449 Size: 6454 BlpI GC'TnA_GC Cuts at: 2252 3871 2252 Size: 1619 4835 Bme1580I G_kGCm'C Cuts at: 1790 4515 5671 1790 Size: 2725 1156 2573 Fragments arranged by size: 2725 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6454 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2351 2929 3445 Size: 416 53 83 399 24 934 578 516 Cuts at: 3445 4185 5143 442 Size: 740 958 1753 Fragments arranged by size: 1753 958 934 740 578 516 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6454 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2160 3140 3446 3462 3508 5114 1239 Size: 921 980 306 16 46 1606 2579 Fragments arranged by size: 2579 1606 980 921 306 46 16 Bpu10I CC'TnA_GC Cuts at: 3375 3375 Size: 6454 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4579 5444 5742 4579 Size: 865 298 5291 Fragments arranged by size: 5291 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5117 882 Size: 617 3618 2219 Fragments arranged by size: 3618 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4245 493 Size: 711 3041 2702 Fragments arranged by size: 3041 2702 711 BsaBI GATnn'nnATC Cuts at: 6448 6448 Size: 6454 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3380 4261 4643 5278 Size: 53 83 186 936 1847 881 382 635 Cuts at: 5278 275 Size: 1451 Fragments arranged by size: 1847 1451 936 881 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4887 5285 5559 5706 6117 4887 Size: 398 274 147 411 5224 Fragments arranged by size: 5224 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2336 2366 3113 3143 1178 Size: 30 165 30 933 30 747 30 4489 Fragments arranged by size: 4489 933 747 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2072 2122 2476 2899 2942 3505 1186 Size: 886 50 354 423 43 563 4135 Fragments arranged by size: 4135 886 563 423 354 50 43 BseYI C'CCAG_C Cuts at: 2894 5657 6153 2894 Size: 2763 496 3195 Fragments arranged by size: 3195 2763 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2487 3133 3341 3558 1163 Size: 573 751 646 208 217 4059 Fragments arranged by size: 4059 751 646 573 217 208 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3904 4665 5693 1540 Size: 275 186 1903 761 1028 2301 Fragments arranged by size: 2301 1903 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2784 2821 3160 4515 4600 5671 2784 Size: 37 339 1355 85 1071 3567 Fragments arranged by size: 3567 1355 1071 339 85 37 BsmI GAATG_Cn' Cuts at: 2707 2750 3356 3553 3965 4058 2707 Size: 43 606 197 412 93 5103 Fragments arranged by size: 5103 606 412 197 93 43 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2339 2784 2821 3160 3773 4515 4600 Size: 399 549 445 37 339 613 742 85 Cuts at: 4600 5671 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 613 549 445 399 339 85 37 BspEI T'CCGG_A Cuts at: 6117 6117 Size: 6454 BspHI T'CATG_A Cuts at: 4343 6073 4343 Size: 1730 4724 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2537 3809 844 Size: 1693 1272 3489 Fragments arranged by size: 3489 1693 1272 BsrBI CCG'CTC Cuts at: 1394 1823 4341 1394 Size: 429 2518 3507 Fragments arranged by size: 3507 2518 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4949 5123 65 Size: 1183 3701 174 1396 Fragments arranged by size: 3701 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2643 5098 5285 1700 Size: 110 833 2455 187 2869 Fragments arranged by size: 2869 2455 833 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6454 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6454 BssSI C'ACGA_G Cuts at: 4514 5526 4514 Size: 1012 5442 BstAPI GCAn_nnn'nTGC Cuts at: 794 2863 794 Size: 2069 4385 BstBI TT'CG_AA Cuts at: 5258 5258 Size: 6454 BstEII G'GTnAC_C Cuts at: 1184 2713 1184 Size: 1529 4925 BstYI r'GATC_y Cuts at: 1972 2427 2494 2773 3283 3784 4151 4536 4553 Size: 455 67 279 510 501 367 385 17 Cuts at: 4553 6005 6091 6103 6449 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 510 501 455 385 367 346 279 86 67 17 12 Bsu36I CC'TnA_GG Cuts at: 2340 2340 Size: 6454 BtgI C'CryG_G Cuts at: 513 1065 2004 2829 3246 3643 513 Size: 552 939 825 417 397 3324 Fragments arranged by size: 3324 939 825 552 417 397 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5715 Fragments arranged by size: 5715 683 56 BtsI GCAGTG_nn' Cuts at: 2263 2819 2878 3596 3970 4764 4784 2263 Size: 556 59 718 374 794 20 3933 Fragments arranged by size: 3933 794 718 556 374 59 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6454 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3351 3386 529 Size: 35 2787 35 3597 Fragments arranged by size: 3597 2787 35 35 DraI TTT'AAA Cuts at: 3758 3758 Size: 6454 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5461 809 Size: 507 4145 1802 Fragments arranged by size: 4145 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2536 3339 3531 Size: 54 1425 50 275 18 706 803 192 Cuts at: 3531 3901 4790 6419 8 Size: 370 889 1629 43 Fragments arranged by size: 1629 1425 889 803 706 370 275 192 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3901 1537 Size: 275 2089 4090 Fragments arranged by size: 4090 2089 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2121 2620 3169 3264 4384 1353 Size: 768 499 549 95 1120 3423 Fragments arranged by size: 3423 1120 768 549 499 95 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5041 5415 5561 387 Size: 1064 138 3452 374 146 1280 Fragments arranged by size: 3452 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 2160 2454 3017 3097 3140 3288 Size: 393 470 58 294 563 80 43 148 Cuts at: 3288 3446 3462 3508 3692 4517 5114 5901 6169 Size: 158 16 46 184 825 597 787 268 Cuts at: 6169 1239 Size: 1524 Fragments arranged by size: 1524 825 787 597 563 470 393 294 268 184 158 148 80 58 46 43 16 EcoNI CCTnn'n_nnAGG Cuts at: 3575 3575 Size: 6454 EcoO109I rG'GnC_Cy Cuts at: 2482 2482 Size: 6454 EcoRI G'AATT_C Cuts at: 3763 4141 3763 Size: 378 6076 FspI TGC'GCA Cuts at: 3355 4960 3355 Size: 1605 4849 FspAI rTGC'GCAy Cuts at: 3355 3355 Size: 6454 HaeII r_GCGC'y Cuts at: 1678 3383 5601 1678 Size: 1705 2218 2531 Fragments arranged by size: 2531 2218 1705 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2036 2068 2336 2368 2479 Size: 32 135 32 464 32 268 32 111 Cuts at: 2479 2511 3082 3114 5095 5127 5169 5201 1373 Size: 32 571 32 1981 32 42 32 2626 Fragments arranged by size: 2626 1981 571 464 268 135 111 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2146 3798 4044 669 Size: 988 281 208 1652 246 3079 Fragments arranged by size: 3079 1652 988 281 246 208 HindIII A'AGCT_T Cuts at: 748 748 Size: 6454 HpaI GTT'AAC Cuts at: 4044 4044 Size: 6454 KasI G'GCGC_C Cuts at: 3379 3379 Size: 6454 KpnI G_GTAC'C Cuts at: 3779 3779 Size: 6454 MfeI C'AATT_G Cuts at: 4053 4053 Size: 6454 MluI A'CGCG_T Cuts at: 6330 6330 Size: 6454 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5568 5752 1305 Size: 4263 184 2007 Fragments arranged by size: 4263 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2538 3533 10 Size: 54 1425 343 706 995 2931 Fragments arranged by size: 2931 1425 995 706 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2638 2765 3635 4412 4771 4930 518 Size: 648 1472 127 870 777 359 159 2042 Fragments arranged by size: 2042 1472 870 777 648 359 159 127 MspA1I CmG'CkG Cuts at: 1606 2841 3508 3870 4547 5695 5940 1606 Size: 1235 667 362 677 1148 245 2120 Fragments arranged by size: 2120 1235 1148 677 667 362 245 NaeI GCC'GGC Cuts at: 2645 2645 Size: 6454 NarI GG'CG_CC Cuts at: 3380 3380 Size: 6454 NcoI C'CATG_G Cuts at: 513 1065 2004 2829 3246 513 Size: 552 939 825 417 3721 Fragments arranged by size: 3721 939 825 552 417 NdeI CA'TA_TG Cuts at: 387 387 Size: 6454 NgoMIV G'CCGG_C Cuts at: 2643 2643 Size: 6454 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6454 NotI GC'GGCC_GC Cuts at: 3901 3901 Size: 6454 NspI r_CATG'y Cuts at: 1132 1229 2310 3354 3812 5357 1132 Size: 97 1081 1044 458 1545 2229 Fragments arranged by size: 2229 1545 1081 1044 458 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6454 PciI A'CATG_T Cuts at: 2306 5353 2306 Size: 3047 3407 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6454 PmeI GTTT'AAAC Cuts at: 3758 3758 Size: 6454 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3113 3145 4523 4555 6036 6068 3113 Size: 32 1378 32 1481 32 3499 Fragments arranged by size: 3499 1481 1378 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2482 2482 Size: 6454 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6454 PsiI TTA'TAA Cuts at: 4024 4024 Size: 6454 PstI C_TGCA'G Cuts at: 830 2826 3291 3806 830 Size: 1996 465 515 3478 Fragments arranged by size: 3478 1996 515 465 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6454 PvuII CAG'CTG Cuts at: 3508 3508 Size: 6454 SalI G'TCGA_C Cuts at: 1936 3796 1936 Size: 1860 4594 SbfI CC_TGCA'GG Cuts at: 3806 3806 Size: 6454 ScaI AGT'ACT Cuts at: 2940 4702 6414 2940 Size: 1762 1712 2980 Fragments arranged by size: 2980 1762 1712 SfcI C'TryA_G Cuts at: 826 1045 2822 3287 3802 4937 5618 5809 826 Size: 219 1777 465 515 1135 681 191 1471 Fragments arranged by size: 1777 1471 1135 681 515 465 219 191 SfoI GGC'GCC Cuts at: 3381 3381 Size: 6454 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6454 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6454 SmaI CCC'GGG Cuts at: 1872 3781 1872 Size: 1909 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4558 5459 5721 820 Size: 197 926 2615 901 262 1553 Fragments arranged by size: 2615 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4245 493 Size: 3752 2702 SpeI A'CTAG_T Cuts at: 152 3481 152 Size: 3329 3125 SphI G_CATG'C Cuts at: 1132 3812 1132 Size: 2680 3774 SspI AAT'ATT Cuts at: 5 52 4378 5 Size: 47 4326 2081 Fragments arranged by size: 4326 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6454 StyI C'CwwG_G Cuts at: 513 1065 2004 2812 2829 3246 3893 513 Size: 552 939 808 17 417 647 3074 Fragments arranged by size: 3074 939 808 647 552 417 17 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2911 4481 4498 4651 4836 5281 325 Size: 1255 1331 1570 17 153 185 445 1498 Fragments arranged by size: 1570 1498 1331 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2938 4700 6412 Size: 274 80 33 51 1443 961 1762 1712 Cuts at: 6412 96 Size: 138 Fragments arranged by size: 1762 1712 1443 961 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2187 2219 6414 6446 2187 Size: 32 4195 32 2195 Fragments arranged by size: 4195 2195 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6454 XbaI T'CTAG_A Cuts at: 3790 3790 Size: 6454 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6454 XmaI C'CCGG_G Cuts at: 1870 3779 1870 Size: 1909 4545 XmnI GAAnn'nnTTC Cuts at: 2283 3030 3110 4583 2283 Size: 747 80 1473 4154 Fragments arranged by size: 4154 1473 747 80 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4262 276 Size: 53 83 186 936 2728 2468 Fragments arranged by size: 2728 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI