(Circular) (Six-base) MAPSORT of: pFN21AE5454.seq Check: 3285 from: 1 to: 5764 pFN21AE5454 5764 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3574 278 Size: 53 83 186 936 2038 2468 Fragments arranged by size: 2468 2038 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3107 1937 Size: 1170 4594 Acc65I G'GTAC_C Cuts at: 3085 3085 Size: 5764 AclI AA'CG_TT Cuts at: 1563 3891 4264 1563 Size: 2328 373 3063 Fragments arranged by size: 3063 2328 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2026 3827 5211 5479 1632 Size: 394 1801 1384 268 1917 Fragments arranged by size: 1917 1801 1384 394 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5567 AflIII A'CryG_T Cuts at: 1203 2104 4663 5640 1203 Size: 901 2559 977 1327 Fragments arranged by size: 2559 1327 977 901 AgeI A'CCGG_T Cuts at: 4595 4595 Size: 5764 AhdI GACnn_n'nnGTC Cuts at: 4493 4493 Size: 5764 AleI CACnn'nnGTG Cuts at: 1166 2525 1166 Size: 1359 4405 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5764 AlwNI CAG_nnn'CTG Cuts at: 3507 5079 3507 Size: 1572 4192 ApaLI G'TGCA_C Cuts at: 3821 4977 3821 Size: 1156 4608 ApoI r'AATT_y Cuts at: 1493 2604 3073 3299 3451 5560 1493 Size: 1111 469 226 152 2109 1697 Fragments arranged by size: 2109 1697 1111 469 226 152 AseI AT'TA_AT Cuts at: 160 1032 4318 160 Size: 872 3286 1606 Fragments arranged by size: 3286 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5764 AvaI C'yCGr_G Cuts at: 1870 1943 3059 3089 1870 Size: 73 1116 30 4545 Fragments arranged by size: 4545 1116 73 30 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2806 2839 2806 Size: 33 5731 BamHI G'GATC_C Cuts at: 3094 3461 3094 Size: 367 5397 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2911 3085 4540 618 Size: 325 217 627 1124 174 1455 1842 Fragments arranged by size: 1842 1455 1124 627 325 217 174 BanII G_rGCy'C Cuts at: 1391 2568 2808 3083 1391 Size: 1177 240 275 4072 Fragments arranged by size: 4072 1177 275 240 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2032 928 Size: 971 133 4660 Fragments arranged by size: 4660 971 133 BbvCI CC'TCA_GC Cuts at: 2160 3051 2160 Size: 891 4873 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3028 3062 3955 3989 1903 Size: 34 1091 34 893 34 3678 Fragments arranged by size: 3678 1091 893 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3656 4866 3656 Size: 1210 4554 BclI T'GATC_A Cuts at: 1548 2293 1548 Size: 745 5019 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4375 136 Size: 107 122 71 631 605 2703 1525 Fragments arranged by size: 2703 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5759 5759 Size: 5764 BlpI GC'TnA_GC Cuts at: 3181 3181 Size: 5764 Bme1580I G_kGCm'C Cuts at: 1790 3825 4981 1790 Size: 2035 1156 2573 Fragments arranged by size: 2573 2035 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5764 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3495 4453 442 Size: 416 53 83 399 24 2078 958 1753 Fragments arranged by size: 2078 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5764 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2983 2983 Size: 5764 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3004 4424 1239 Size: 1765 1420 2579 Fragments arranged by size: 2579 1765 1420 Bpu10I CC'TnA_GC Cuts at: 2160 3051 2160 Size: 891 4873 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2076 3889 4754 5052 2076 Size: 1813 865 298 2788 Fragments arranged by size: 2788 1813 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2552 4427 882 Size: 617 1053 1875 2219 Fragments arranged by size: 2219 1875 1053 617 BsaAI yAC'GTr Cuts at: 493 1204 3555 493 Size: 711 2351 2702 Fragments arranged by size: 2702 2351 711 BsaBI GATnn'nnATC Cuts at: 5758 5758 Size: 5764 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3571 3953 4588 275 Size: 53 83 186 936 2038 382 635 1451 Fragments arranged by size: 2038 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4197 4595 4869 5016 5427 4197 Size: 398 274 147 411 4534 Fragments arranged by size: 4534 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5539 Fragments arranged by size: 5539 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2863 1186 Size: 1677 4087 BseYI C'CCAG_C Cuts at: 4967 5463 4967 Size: 496 5268 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2378 2516 1163 Size: 573 642 138 4411 Fragments arranged by size: 4411 642 573 138 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3214 3975 5003 1540 Size: 275 186 1213 761 1028 2301 Fragments arranged by size: 2301 1213 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2568 3825 3910 4981 2568 Size: 1257 85 1071 3351 Fragments arranged by size: 3351 1257 1071 85 BsmI GAATG_Cn' Cuts at: 2171 3275 3368 2171 Size: 1104 93 4567 Fragments arranged by size: 4567 1104 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2568 2808 3083 3825 3910 4981 1391 Size: 399 778 240 275 742 85 1071 2174 Fragments arranged by size: 2174 1071 778 742 399 275 240 85 BspEI T'CCGG_A Cuts at: 5427 5427 Size: 5764 BspHI T'CATG_A Cuts at: 3653 5383 3653 Size: 1730 4034 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2626 3119 844 Size: 1782 493 3489 Fragments arranged by size: 3489 1782 493 BsrBI CCG'CTC Cuts at: 1394 1823 3651 1394 Size: 429 1828 3507 Fragments arranged by size: 3507 1828 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2210 2377 2997 4259 4433 65 Size: 1183 962 167 620 1262 174 1396 Fragments arranged by size: 1396 1262 1183 962 620 174 167 BsrFI r'CCGG_y Cuts at: 1700 1810 4408 4595 1700 Size: 110 2598 187 2869 Fragments arranged by size: 2869 2598 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5764 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5764 BssSI C'ACGA_G Cuts at: 3824 4836 3824 Size: 1012 4752 BstAPI GCAn_nnn'nTGC Cuts at: 794 3020 794 Size: 2226 3538 BstBI TT'CG_AA Cuts at: 4568 4568 Size: 5764 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5764 BstYI r'GATC_y Cuts at: 1972 3094 3461 3846 3863 5315 5401 5413 5759 Size: 1122 367 385 17 1452 86 12 346 Cuts at: 5759 1972 Size: 1977 Fragments arranged by size: 1977 1452 1122 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5212 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5025 Fragments arranged by size: 5025 683 56 BtsI GCAGTG_nn' Cuts at: 2361 3009 3280 4074 4094 2361 Size: 648 271 794 20 4031 Fragments arranged by size: 4031 794 648 271 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5764 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5729 DraI TTT'AAA Cuts at: 2721 3068 2721 Size: 347 5417 DraIII CAC_nnn'GTG Cuts at: 2416 2416 Size: 5764 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4771 809 Size: 507 3455 1802 Fragments arranged by size: 3455 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3211 4100 5729 Size: 54 1425 50 275 18 1381 889 1629 Cuts at: 5729 8 Size: 43 Fragments arranged by size: 1629 1425 1381 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3211 1537 Size: 275 1399 4090 Fragments arranged by size: 4090 1399 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2470 3694 1353 Size: 1117 1224 3423 Fragments arranged by size: 3423 1224 1117 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4351 4725 4871 387 Size: 1064 138 2762 374 146 1280 Fragments arranged by size: 2762 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2566 2566 Size: 5764 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2026 3004 3827 4424 5211 5479 1239 Size: 393 394 978 823 597 787 268 1524 Fragments arranged by size: 1524 978 823 787 597 394 393 268 EcoRI G'AATT_C Cuts at: 3073 3451 3073 Size: 378 5386 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3059 3059 Size: 5764 FspI TGC'GCA Cuts at: 4270 4270 Size: 5764 HaeII r_GCGC'y Cuts at: 1678 4911 1678 Size: 3233 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2285 2317 2991 3023 4405 Size: 32 135 32 713 32 674 32 1382 Cuts at: 4405 4437 4479 4511 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1382 713 674 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2827 3108 3354 669 Size: 988 281 889 281 246 3079 Fragments arranged by size: 3079 988 889 281 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5764 HpaI GTT'AAC Cuts at: 2827 3354 2827 Size: 527 5237 KpnI G_GTAC'C Cuts at: 3089 3089 Size: 5764 MfeI C'AATT_G Cuts at: 3363 3363 Size: 5764 MluI A'CGCG_T Cuts at: 5640 5640 Size: 5764 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4878 5062 1305 Size: 3573 184 2007 Fragments arranged by size: 3573 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3942 Fragments arranged by size: 3942 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2525 3722 4081 4240 518 Size: 648 1359 1197 359 159 2042 Fragments arranged by size: 2042 1359 1197 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2299 2403 3180 3857 5005 5250 1606 Size: 693 104 777 677 1148 245 2120 Fragments arranged by size: 2120 1148 777 693 677 245 104 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5212 NdeI CA'TA_TG Cuts at: 387 387 Size: 5764 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5764 NotI GC'GGCC_GC Cuts at: 3211 3211 Size: 5764 NspI r_CATG'y Cuts at: 1132 1229 2108 3122 4667 1132 Size: 97 879 1014 1545 2229 Fragments arranged by size: 2229 1545 1014 879 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5764 PciI A'CATG_T Cuts at: 2104 4663 2104 Size: 2559 3205 PflMI CCAn_nnn'nTGG Cuts at: 1108 2704 1108 Size: 1596 4168 PmeI GTTT'AAAC Cuts at: 3068 3068 Size: 5764 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3833 3865 5346 5378 3833 Size: 32 1481 32 4219 Fragments arranged by size: 4219 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5764 PsiI TTA'TAA Cuts at: 3334 3334 Size: 5764 PstI C_TGCA'G Cuts at: 830 3116 830 Size: 2286 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5764 PvuII CAG'CTG Cuts at: 2299 2299 Size: 5764 SacI G_AGCT'C Cuts at: 2568 2568 Size: 5764 SalI G'TCGA_C Cuts at: 1936 3106 1936 Size: 1170 4594 SbfI CC_TGCA'GG Cuts at: 3116 3116 Size: 5764 ScaI AGT'ACT Cuts at: 4012 5724 4012 Size: 1712 4052 SexAI A'CCwGG_T Cuts at: 2412 2490 2412 Size: 78 5686 SfcI C'TryA_G Cuts at: 826 1045 3112 4247 4928 5119 826 Size: 219 2067 1135 681 191 1471 Fragments arranged by size: 2067 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5764 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5764 SmaI CCC'GGG Cuts at: 1872 3091 1872 Size: 1219 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2091 3868 4769 5031 820 Size: 197 926 148 1777 901 262 1553 Fragments arranged by size: 1777 1553 926 901 262 197 148 SnaBI TAC'GTA Cuts at: 493 3555 493 Size: 3062 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5764 SphI G_CATG'C Cuts at: 1132 3122 1132 Size: 1990 3774 SspI AAT'ATT Cuts at: 5 52 2847 3688 5 Size: 47 2795 841 2081 Fragments arranged by size: 2795 2081 841 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5764 StyI C'CwwG_G Cuts at: 513 1065 3203 513 Size: 552 2138 3074 Fragments arranged by size: 3074 2138 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3791 3808 3961 4146 4591 325 Size: 1255 2211 17 153 185 445 1498 Fragments arranged by size: 2211 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4010 5722 96 Size: 274 80 33 51 1443 2033 1712 138 Fragments arranged by size: 2033 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5724 5756 5724 Size: 32 5732 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5764 XbaI T'CTAG_A Cuts at: 3100 3100 Size: 5764 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2488 2488 Size: 5764 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5764 XmaI C'CCGG_G Cuts at: 1870 3089 1870 Size: 1219 4545 XmnI GAAnn'nnTTC Cuts at: 3893 3893 Size: 5764 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3572 276 Size: 53 83 186 936 2038 2468 Fragments arranged by size: 2468 2038 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI