Restriction map for pFN21AE5454
 (Circular) (Six-base) MAPSORT of: pFN21AE5454.seq  Check: 3285  from: 1  to: 5764



pFN21AE5454  5764 bp

 With 182 enzymes: SgfI * 

                        July 19, 2019 13:42  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3574     278 
   Size:         53      83     186     936    2038    2468

  Fragments arranged by size: 

                2468    2038     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3107    1937 
   Size:       1170    4594
 
Acc65I G'GTAC_C

Cuts at:   3085    3085 
   Size:       5764
 
AclI AA'CG_TT

Cuts at:   1563    3891    4264    1563 
   Size:       2328     373    3063

  Fragments arranged by size: 

                3063    2328     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2026    3827    5211    5479    1632 
   Size:        394    1801    1384     268    1917

  Fragments arranged by size: 

                1917    1801    1384     394     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5567
 
AflIII A'CryG_T

Cuts at:   1203    2104    4663    5640    1203 
   Size:        901    2559     977    1327

  Fragments arranged by size: 

                2559    1327     977     901
 
AgeI A'CCGG_T

Cuts at:   4595    4595 
   Size:       5764
 
AhdI GACnn_n'nnGTC

Cuts at:   4493    4493 
   Size:       5764
 
AleI CACnn'nnGTG

Cuts at:   1166    2525    1166 
   Size:       1359    4405
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5764
 
AlwNI CAG_nnn'CTG

Cuts at:   3507    5079    3507 
   Size:       1572    4192
 
ApaLI G'TGCA_C

Cuts at:   3821    4977    3821 
   Size:       1156    4608
 
ApoI r'AATT_y

Cuts at:   1493    2604    3073    3299    3451    5560    1493 
   Size:       1111     469     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1111     469     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4318     160 
   Size:        872    3286    1606

  Fragments arranged by size: 

                3286    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5764
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3059    3089    1870 
   Size:         73    1116      30    4545

  Fragments arranged by size: 

                4545    1116      73      30
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   2806    2839    2806 
   Size:         33    5731
 
BamHI G'GATC_C

Cuts at:   3094    3461    3094 
   Size:        367    5397
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2911    3085    4540     618 
   Size:        325     217     627    1124     174    1455    1842

  Fragments arranged by size: 

                1842    1455    1124     627     325     217     174
 
BanII G_rGCy'C

Cuts at:   1391    2568    2808    3083    1391 
   Size:       1177     240     275    4072

  Fragments arranged by size: 

                4072    1177     275     240
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2032     928 
   Size:        971     133    4660

  Fragments arranged by size: 

                4660     971     133
 
BbvCI CC'TCA_GC

Cuts at:   2160    3051    2160 
   Size:        891    4873
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3028    3062    3955    3989    1903 
   Size:         34    1091      34     893      34    3678

  Fragments arranged by size: 

                3678    1091     893      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3656    4866    3656 
   Size:       1210    4554
 
BclI T'GATC_A

Cuts at:   1548    2293    1548 
   Size:        745    5019
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4375     136 
   Size:        107     122      71     631     605    2703    1525

  Fragments arranged by size: 

                2703    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5759    5759 
   Size:       5764
 
BlpI GC'TnA_GC

Cuts at:   3181    3181 
   Size:       5764
 
Bme1580I G_kGCm'C

Cuts at:   1790    3825    4981    1790 
   Size:       2035    1156    2573

  Fragments arranged by size: 

                2573    2035    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5764
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3495    4453     442 
   Size:        416      53      83     399      24    2078     958    1753

  Fragments arranged by size: 

                2078    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5764
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2983    2983 
   Size:       5764
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3004    4424    1239 
   Size:       1765    1420    2579

  Fragments arranged by size: 

                2579    1765    1420
 
Bpu10I CC'TnA_GC

Cuts at:   2160    3051    2160 
   Size:        891    4873
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2076    3889    4754    5052    2076 
   Size:       1813     865     298    2788

  Fragments arranged by size: 

                2788    1813     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2552    4427     882 
   Size:        617    1053    1875    2219

  Fragments arranged by size: 

                2219    1875    1053     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3555     493 
   Size:        711    2351    2702

  Fragments arranged by size: 

                2702    2351     711
 
BsaBI GATnn'nnATC

Cuts at:   5758    5758 
   Size:       5764
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3571    3953    4588     275 
   Size:         53      83     186     936    2038     382     635    1451

  Fragments arranged by size: 

                2038    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4197    4595    4869    5016    5427    4197 
   Size:        398     274     147     411    4534

  Fragments arranged by size: 

                4534     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5539

  Fragments arranged by size: 

                5539     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2863    1186 
   Size:       1677    4087
 
BseYI C'CCAG_C

Cuts at:   4967    5463    4967 
   Size:        496    5268
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2378    2516    1163 
   Size:        573     642     138    4411

  Fragments arranged by size: 

                4411     642     573     138
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3214    3975    5003    1540 
   Size:        275     186    1213     761    1028    2301

  Fragments arranged by size: 

                2301    1213    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2568    3825    3910    4981    2568 
   Size:       1257      85    1071    3351

  Fragments arranged by size: 

                3351    1257    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2171    3275    3368    2171 
   Size:       1104      93    4567

  Fragments arranged by size: 

                4567    1104      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2568    2808    3083    3825    3910    4981    1391 
   Size:        399     778     240     275     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     778     742     399     275     240      85
            
 
BspEI T'CCGG_A

Cuts at:   5427    5427 
   Size:       5764
 
BspHI T'CATG_A

Cuts at:   3653    5383    3653 
   Size:       1730    4034
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2626    3119     844 
   Size:       1782     493    3489

  Fragments arranged by size: 

                3489    1782     493
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3651    1394 
   Size:        429    1828    3507

  Fragments arranged by size: 

                3507    1828     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2210    2377    2997    4259    4433      65 
   Size:       1183     962     167     620    1262     174    1396

  Fragments arranged by size: 

                1396    1262    1183     962     620     174     167
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4408    4595    1700 
   Size:        110    2598     187    2869

  Fragments arranged by size: 

                2869    2598     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5764
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5764
 
BssSI C'ACGA_G

Cuts at:   3824    4836    3824 
   Size:       1012    4752
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    3020     794 
   Size:       2226    3538
 
BstBI TT'CG_AA

Cuts at:   4568    4568 
   Size:       5764
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5764
 
BstYI r'GATC_y

Cuts at:   1972    3094    3461    3846    3863    5315    5401    5413    5759 
   Size:       1122     367     385      17    1452      86      12     346

Cuts at:   5759    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452    1122     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    5212
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5025

  Fragments arranged by size: 

                5025     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2361    3009    3280    4074    4094    2361 
   Size:        648     271     794      20    4031

  Fragments arranged by size: 

                4031     794     648     271      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5764
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5729
 
DraI TTT'AAA

Cuts at:   2721    3068    2721 
   Size:        347    5417
 
DraIII CAC_nnn'GTG

Cuts at:   2416    2416 
   Size:       5764
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4771     809 
   Size:        507    3455    1802

  Fragments arranged by size: 

                3455    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3211    4100    5729 
   Size:         54    1425      50     275      18    1381     889    1629

Cuts at:   5729       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1381     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3211    1537 
   Size:        275    1399    4090

  Fragments arranged by size: 

                4090    1399     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2470    3694    1353 
   Size:       1117    1224    3423

  Fragments arranged by size: 

                3423    1224    1117
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4351    4725    4871     387 
   Size:       1064     138    2762     374     146    1280

  Fragments arranged by size: 

                2762    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2566    2566 
   Size:       5764
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2026    3004    3827    4424    5211    5479    1239 
   Size:        393     394     978     823     597     787     268    1524

  Fragments arranged by size: 

                1524     978     823     787     597     394     393     268
            
 
EcoRI G'AATT_C

Cuts at:   3073    3451    3073 
   Size:        378    5386
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3059    3059 
   Size:       5764
 
FspI TGC'GCA

Cuts at:   4270    4270 
   Size:       5764
 
HaeII r_GCGC'y

Cuts at:   1678    4911    1678 
   Size:       3233    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2285    2317    2991    3023    4405 
   Size:         32     135      32     713      32     674      32    1382

Cuts at:   4405    4437    4479    4511    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1382     713     674     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2827    3108    3354     669 
   Size:        988     281     889     281     246    3079

  Fragments arranged by size: 

                3079     988     889     281     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5764
 
HpaI GTT'AAC

Cuts at:   2827    3354    2827 
   Size:        527    5237
 
KpnI G_GTAC'C

Cuts at:   3089    3089 
   Size:       5764
 
MfeI C'AATT_G

Cuts at:   3363    3363 
   Size:       5764
 
MluI A'CGCG_T

Cuts at:   5640    5640 
   Size:       5764
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4878    5062    1305 
   Size:       3573     184    2007

  Fragments arranged by size: 

                3573    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3942

  Fragments arranged by size: 

                3942    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2525    3722    4081    4240     518 
   Size:        648    1359    1197     359     159    2042

  Fragments arranged by size: 

                2042    1359    1197     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2299    2403    3180    3857    5005    5250    1606 
   Size:        693     104     777     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     777     693     677     245     104
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5212
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5764
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5764
 
NotI GC'GGCC_GC

Cuts at:   3211    3211 
   Size:       5764
 
NspI r_CATG'y

Cuts at:   1132    1229    2108    3122    4667    1132 
   Size:         97     879    1014    1545    2229

  Fragments arranged by size: 

                2229    1545    1014     879      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5764
 
PciI A'CATG_T

Cuts at:   2104    4663    2104 
   Size:       2559    3205
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2704    1108 
   Size:       1596    4168
 
PmeI GTTT'AAAC

Cuts at:   3068    3068 
   Size:       5764
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3833    3865    5346    5378    3833 
   Size:         32    1481      32    4219

  Fragments arranged by size: 

                4219    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5764
 
PsiI TTA'TAA

Cuts at:   3334    3334 
   Size:       5764
 
PstI C_TGCA'G

Cuts at:    830    3116     830 
   Size:       2286    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5764
 
PvuII CAG'CTG

Cuts at:   2299    2299 
   Size:       5764
 
SacI G_AGCT'C

Cuts at:   2568    2568 
   Size:       5764
 
SalI G'TCGA_C

Cuts at:   1936    3106    1936 
   Size:       1170    4594
 
SbfI CC_TGCA'GG

Cuts at:   3116    3116 
   Size:       5764
 
ScaI AGT'ACT

Cuts at:   4012    5724    4012 
   Size:       1712    4052
 
SexAI A'CCwGG_T

Cuts at:   2412    2490    2412 
   Size:         78    5686
 
SfcI C'TryA_G

Cuts at:    826    1045    3112    4247    4928    5119     826 
   Size:        219    2067    1135     681     191    1471

  Fragments arranged by size: 

                2067    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5764
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5764
 
SmaI CCC'GGG

Cuts at:   1872    3091    1872 
   Size:       1219    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2091    3868    4769    5031     820 
   Size:        197     926     148    1777     901     262    1553

  Fragments arranged by size: 

                1777    1553     926     901     262     197     148
 
SnaBI TAC'GTA

Cuts at:    493    3555     493 
   Size:       3062    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5764
 
SphI G_CATG'C

Cuts at:   1132    3122    1132 
   Size:       1990    3774
 
SspI AAT'ATT

Cuts at:      5      52    2847    3688       5 
   Size:         47    2795     841    2081

  Fragments arranged by size: 

                2795    2081     841      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5764
 
StyI C'CwwG_G

Cuts at:    513    1065    3203     513 
   Size:        552    2138    3074

  Fragments arranged by size: 

                3074    2138     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3791    3808    3961    4146    4591     325 
   Size:       1255    2211      17     153     185     445    1498

  Fragments arranged by size: 

                2211    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4010    5722      96 
   Size:        274      80      33      51    1443    2033    1712     138

  Fragments arranged by size: 

                2033    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5724    5756    5724 
   Size:         32    5732
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5764
 
XbaI T'CTAG_A

Cuts at:   3100    3100 
   Size:       5764
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2488    2488 
   Size:       5764
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5764
 
XmaI C'CCGG_G

Cuts at:   1870    3089    1870 
   Size:       1219    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3893    3893 
   Size:       5764
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3572     276 
   Size:         53      83     186     936    2038    2468

  Fragments arranged by size: 

                2468    2038     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI     BaeI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI
     NspI     PasI     PciI    PflMI     PmeI     PpiI    PshAI     PsiI
     PstI     PvuI    PvuII     SacI     SalI     SbfI     ScaI    SexAI
     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BbeI    BfrBI
    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I    EcoNI EcoO109I    EcoRV
     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     NsiI
     PacI     PfoI     PmlI    PpuMI   PspOMI    PspXI     PsrI    RsrII
    SacII    SanDI     SapI     SfiI     SfoI     SrfI     SwaI
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