Restriction map for
pFN21AE6786
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# NO CUT ENZYMES ( 99 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI AocI Aor51HI AscI AseI
AsnI Asp700I Asp718I AvrII BamHI
BbrPI BbvCI BciVI BclI BfrI
BglII BlnI BsaAI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DrdI EarI Eco47III Eco81I
EcoO65I EcoT22I FbaI FseI HindIII
KpnI Ksp632I KspI MfeI MroI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SapI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Van91I XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccI 459 GTCGAC GT^[AC][GT]AC
AcsI 1777 GAATTC [GA]^AATT[CT]
AcyI 1105 GGCGCC G[GA]^CG[CT]C
AflIII 1227 ACGCGT A^C[GA][CT]GT
AhdI 451 GACCGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1056 GGGCCC GGGCC^C
ApoI 1777 GAATTC [GA]^AATT[CT]
AspEI 451 GACCGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 10 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 932 TGCGCA TGC^GCA
BbeI 1105 GGCGCC GGCGC^C
BfaI 1417 CTAG C^TAG
BglI 1568 GCCTCTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1158 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1158 GCTGAGC GC^T[ACGT]AGC
BsaBI 588 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1105 GGCGCC G[GA]^CG[CT]C
BsaI 1260 GGTCTC GGTCTC(1/5)
BsaWI 1871 ACCGGA [AT]^CCGG[AT]
BsiEI 1124 CGGCCG CG[GA][CT]^CG
BsmBI 1859 GAGACG CGTCTC(1/5)
BspMI 1374 GCAGGT ACCTGC(4/8)
BstXI 314 CCAAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1532 AGATCC [GA]^GATC[CT]
CelII 1158 GCTGAGC GC^T[ACGT]AGC
DraIII 798 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1109 CCACGG C^C[GA][CT]GG
EagI 1124 CGGCCG C^GGCCG
Eam1105I 451 GACCGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1124 CGGCCG C^GGCCG
Eco52I 1124 CGGCCG C^GGCCG
EcoNI 1194 CCTGCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1777 GAATTC G^AATTC
EcoRV 1430 GATATC GAT^ATC
EcoT14I 221 CCAAGG C^C[AT][AT]GG
FspI 932 TGCGCA TGC^GCA
HaeII 1105 GGCGCC [GA]GCGC^[CT]
Hin1I 1105 GGCGCC G[GA]^CG[CT]C
HpaI 246 GTTAAC GTT^AAC
KasI 1105 GGCGCC G^GCGCC
MaeI 1417 CTAG C^TAG
MamI 588 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1532 AGATCC [GA]^GATC[CT]
MluI 1227 ACGCGT A^CGCGT
NarI 1105 GGCGCC GG^CGCC
NruI 6 TCGCGA TCG^CGA
PflFI 10 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1309 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 459 GTCGAC G^TCGAC
SexAI 11 ACCAGGT A^CC[AT]GGT
SfcI 984 CTACAG C^T[GA][CT]AG
SfoI 1105 GGCGCC GGC^GCC
SmlI 85 CTCAAG C^T[CT][GA]AG
SphI 1685 GCATGC GCATG^C
StuI 980 AGGCCT AGG^CCT
StyI 221 CCAAGG C^C[AT][AT]GG
Tsp45I 1792 GTGAC ^GT[GC]AC
Tth111I 10 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1416 TCTAGA T^CTAGA
XhoII 1532 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
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AfaI 707 GTAC GT^AC
1527 GTAC
Alw44I 1245 GTGCAC G^TGCAC
1735 GTGCAC
AlwI 963 GATCC GGATC(4/5)
1533 GATCC
ApaLI 1245 GTGCAC G^TGCAC
1735 GTGCAC
BalI 474 TGGCCA TGG^CCA
639 TGGCCA
BanI 1105 GGCGCC G^G[CT][GA]CC
1576 GGCACC
BbsI 409 GAAGAC GAAGAC(2/6)
1643 GAAGAC
BcgI 567 GCAGAACTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1440 GCAAAGAAATCG
BpuAI 409 GAAGAC GAAGAC(2/6)
1643 GAAGAC
BspHI 1323 TCATGA T^CATGA
1631 TCATGA
BstF5I 391 CATCC GGATG(2/0)
936 CATCC
FokI 391 CATCC GGATG(9/13)
936 CATCC
HphI 528 TCACC GGTGA(8/7)
797 TCACC
MaeIII 826 GTTAC ^GT[ACGT]AC
1792 GTGAC
MboII 409 GAAGA GAAGA(8/7)
1643 GAAGA
MluNI 474 TGGCCA TGG^CCA
639 TGGCCA
MscI 474 TGGCCA TGG^CCA
639 TGGCCA
NspI 1685 GCATGC [GA]CATG^[CT]
1754 ACATGC
PpuMI 1205 GGGACCT [GA]G^G[AT]CC[CT]
1717 AGGACCC
RcaI 1323 TCATGA T^CATGA
1631 TCATGA
RsaI 707 GTAC GT^AC
1527 GTAC
SfaNI 593 GCATC GCATC(5/9)
935 GCATC
SmaI 1213 CCCGGG CCC^GGG
1669 CCCGGG
XmaCI 1213 CCCGGG C^CCGGG
1669 CCCGGG
XmaI 1213 CCCGGG C^CCGGG
1669 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AluI AlwNI AspHI
AvaI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrI BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII FauI Fnu4HI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII ItaI MaeII MboI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PvuII Sau3AI
Sau96I ScrFI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I