(Circular) (Six-base) MAPSORT of: pFN21AE6786.seq Check: 4470 from: 1 to: 6592 pFN21AE6786 6592 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3370 3370 Size: 6592 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4402 278 Size: 53 83 186 936 2866 2468 Fragments arranged by size: 2866 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2465 3935 1937 Size: 528 1470 4594 Fragments arranged by size: 4594 1470 528 Acc65I G'GTAC_C Cuts at: 3913 3913 Size: 6592 AclI AA'CG_TT Cuts at: 1563 4719 5092 1563 Size: 3156 373 3063 Fragments arranged by size: 3156 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2918 4655 6039 6307 1632 Size: 1286 1737 1384 268 1917 Fragments arranged by size: 1917 1737 1384 1286 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6395 AflIII A'CryG_T Cuts at: 1203 3232 5491 6468 1203 Size: 2029 2259 977 1327 Fragments arranged by size: 2259 2029 1327 977 AgeI A'CCGG_T Cuts at: 5423 5423 Size: 6592 AhdI GACnn_n'nnGTC Cuts at: 2461 5321 2461 Size: 2860 3732 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6592 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2088 2390 2772 3707 1184 Size: 904 302 382 935 4069 Fragments arranged by size: 4069 935 904 382 302 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3542 3574 3542 Size: 32 6560 AlwNI CAG_nnn'CTG Cuts at: 2377 2504 2956 3107 3658 4335 5907 2377 Size: 127 452 151 551 677 1572 3062 Fragments arranged by size: 3062 1572 677 551 452 151 127 ApaI G_GGCC'C Cuts at: 3065 3065 Size: 6592 ApaLI G'TGCA_C Cuts at: 3250 3740 4649 5805 3250 Size: 490 909 1156 4037 Fragments arranged by size: 4037 1156 909 490 ApoI r'AATT_y Cuts at: 1493 3782 3901 4127 4279 6388 1493 Size: 2289 119 226 152 2109 1697 Fragments arranged by size: 2289 2109 1697 226 152 119 AseI AT'TA_AT Cuts at: 160 1032 5146 160 Size: 872 4114 1606 Fragments arranged by size: 4114 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6592 AvaI C'yCGr_G Cuts at: 1870 1943 2580 2998 3011 3065 3218 3674 3917 Size: 73 637 418 13 54 153 456 243 Cuts at: 3917 1870 Size: 4545 Fragments arranged by size: 4545 637 456 418 243 153 73 54 13 BamHI G'GATC_C Cuts at: 3922 4289 3922 Size: 367 6225 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3110 3581 3913 5368 618 Size: 325 217 627 1323 471 332 1455 1842 Fragments arranged by size: 1842 1455 1323 627 471 332 325 217 BanII G_rGCy'C Cuts at: 1391 3033 3065 3080 3150 3911 1391 Size: 1642 32 15 70 761 4072 Fragments arranged by size: 4072 1642 761 70 32 15 BbeI G_GCGC'C Cuts at: 3114 3114 Size: 6592 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2421 3655 928 Size: 971 522 1234 3865 Fragments arranged by size: 3865 1234 971 522 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2561 2595 3434 3468 4783 4817 1903 Size: 34 624 34 839 34 1315 34 3678 Fragments arranged by size: 3678 1315 839 624 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4484 5694 4484 Size: 1210 5382 BclI T'GATC_A Cuts at: 1548 1548 Size: 6592 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3579 5203 136 Size: 107 122 71 631 605 1907 1624 1525 Fragments arranged by size: 1907 1624 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6587 6587 Size: 6592 BlpI GC'TnA_GC Cuts at: 3164 4009 3164 Size: 845 5747 Bme1580I G_kGCm'C Cuts at: 1790 2655 3065 3254 3584 3744 4653 5809 1790 Size: 865 410 189 330 160 909 1156 2573 Fragments arranged by size: 2573 1156 909 865 410 330 189 160 BmgBI CAC'GTC Cuts at: 1318 2170 2463 1318 Size: 852 293 5447 Fragments arranged by size: 5447 852 293 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4323 5281 442 Size: 416 53 83 399 24 2906 958 1753 Fragments arranged by size: 2906 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6592 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2108 2156 2837 2933 3134 3253 3845 5252 Size: 869 48 681 96 201 119 592 1407 Cuts at: 5252 1239 Size: 2579 Fragments arranged by size: 2579 1407 869 681 592 201 119 96 48 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2075 4717 5582 5880 2075 Size: 2642 865 298 2787 Fragments arranged by size: 2787 2642 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3271 5255 882 Size: 617 1772 1984 2219 Fragments arranged by size: 2219 1984 1772 617 BsaAI yAC'GTr Cuts at: 493 1204 4383 493 Size: 711 3179 2702 Fragments arranged by size: 3179 2702 711 BsaBI GATnn'nnATC Cuts at: 2597 6586 2597 Size: 3989 2603 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3111 4399 4781 5416 Size: 53 83 186 936 1578 1288 382 635 Cuts at: 5416 275 Size: 1451 Fragments arranged by size: 1578 1451 1288 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3876 5025 5423 5697 5844 6255 3876 Size: 1149 398 274 147 411 4213 Fragments arranged by size: 4213 1149 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2877 2907 3818 3848 3855 Size: 30 165 30 1474 30 911 30 7 Cuts at: 3855 3866 3885 3896 1178 Size: 11 19 11 3874 Fragments arranged by size: 3874 1474 911 165 30 30 30 30 19 11 11 7 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2375 2653 2656 2801 1186 Size: 1189 278 3 145 4977 Fragments arranged by size: 4977 1189 278 145 3 BseYI C'CCAG_C Cuts at: 5795 6291 5795 Size: 496 6096 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2150 2732 2942 3404 1163 Size: 573 414 582 210 462 4351 Fragments arranged by size: 4351 582 573 462 414 210 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3132 4042 4803 5831 1540 Size: 275 186 1131 910 761 1028 2301 Fragments arranged by size: 2301 1131 1028 910 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2142 3005 3254 3744 4653 4738 5809 2142 Size: 863 249 490 909 85 1071 2925 Fragments arranged by size: 2925 1071 909 863 490 249 85 BsmI GAATG_Cn' Cuts at: 4103 4196 4103 Size: 93 6499 BsmBI CGTCTCn'nnnn_ Cuts at: 3858 3858 Size: 6592 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2142 2655 3005 3033 3065 3080 3150 Size: 399 352 513 350 28 32 15 70 Cuts at: 3150 3254 3584 3744 3911 4653 4738 5809 1391 Size: 104 330 160 167 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 513 399 352 350 330 167 160 104 85 70 32 28 15 BspEI T'CCGG_A Cuts at: 6255 6255 Size: 6592 BspHI T'CATG_A Cuts at: 3328 3636 4481 6211 3328 Size: 308 845 1730 3709 Fragments arranged by size: 3709 1730 845 308 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3370 3947 844 Size: 2526 577 3489 Fragments arranged by size: 3489 2526 577 BsrBI CCG'CTC Cuts at: 1394 1823 2496 2947 3729 4479 1394 Size: 429 673 451 782 750 3507 Fragments arranged by size: 3507 782 750 673 451 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5087 5261 65 Size: 1183 3839 174 1396 Fragments arranged by size: 3839 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5236 5423 1700 Size: 110 3426 187 2869 Fragments arranged by size: 3426 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6592 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6592 BssSI C'ACGA_G Cuts at: 4652 5664 4652 Size: 1012 5580 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6592 BstBI TT'CG_AA Cuts at: 5396 5396 Size: 6592 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6592 BstXI CCAn_nnnn'nTGG Cuts at: 2326 2326 Size: 6592 BstYI r'GATC_y Cuts at: 1972 3537 3922 4289 4674 4691 6143 6229 6241 Size: 1565 385 367 385 17 1452 86 12 Cuts at: 6241 6587 1972 Size: 346 1977 Fragments arranged by size: 1977 1565 1452 385 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3114 513 Size: 552 2049 3991 Fragments arranged by size: 3991 2049 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3189 487 Size: 683 56 1963 3890 Fragments arranged by size: 3890 1963 683 56 BtsI GCAGTG_nn' Cuts at: 4108 4902 4922 4108 Size: 794 20 5778 Fragments arranged by size: 5778 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6592 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6557 DraI TTT'AAA Cuts at: 3896 3896 Size: 6592 DraIII CAC_nnn'GTG Cuts at: 2808 2808 Size: 6592 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5599 809 Size: 507 4283 1802 Fragments arranged by size: 4283 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2148 2479 2644 Size: 54 1425 50 275 18 318 331 165 Cuts at: 2644 3117 3129 3186 4039 4928 6557 8 Size: 473 12 57 853 889 1629 43 Fragments arranged by size: 1629 1425 889 853 473 331 318 275 165 57 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 3129 4039 1537 Size: 275 1317 910 4090 Fragments arranged by size: 4090 1317 910 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4522 1353 Size: 3169 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2732 3550 5179 5553 5699 387 Size: 1064 138 1143 818 1629 374 146 1280 Fragments arranged by size: 1629 1280 1143 1064 818 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2108 2156 2837 2918 2933 3134 3253 Size: 393 476 48 681 81 15 201 119 Cuts at: 3253 3845 4655 5252 6039 6307 1239 Size: 592 810 597 787 268 1524 Fragments arranged by size: 1524 810 787 681 597 592 476 393 268 201 119 81 48 15 EcoNI CCTnn'n_nnAGG Cuts at: 3203 3203 Size: 6592 EcoO109I rG'GnC_Cy Cuts at: 3037 3062 3211 3723 3037 Size: 25 149 512 5906 Fragments arranged by size: 5906 512 149 25 EcoRI G'AATT_C Cuts at: 3782 3901 4279 3782 Size: 119 378 6095 Fragments arranged by size: 6095 378 119 EcoRV GAT'ATC Cuts at: 3437 3437 Size: 6592 FspI TGC'GCA Cuts at: 2939 5098 2939 Size: 2159 4433 HaeII r_GCGC'y Cuts at: 1678 3114 5739 1678 Size: 1436 2625 2531 Fragments arranged by size: 2625 2531 1436 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2447 2479 2877 2909 2959 Size: 32 135 32 875 32 398 32 50 Cuts at: 2959 2991 3529 3561 3866 3898 5233 5265 5307 Size: 32 538 32 305 32 1335 32 42 Cuts at: 5307 5339 1373 Size: 32 2626 Fragments arranged by size: 2626 1335 875 538 398 305 135 50 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2253 2466 3874 3936 4182 669 Size: 988 281 315 213 1408 62 246 3079 Fragments arranged by size: 3079 1408 988 315 281 246 213 62 HindIII A'AGCT_T Cuts at: 748 748 Size: 6592 HpaI GTT'AAC Cuts at: 2253 4182 2253 Size: 1929 4663 KasI G'GCGC_C Cuts at: 3110 3110 Size: 6592 KpnI G_GTAC'C Cuts at: 3917 3917 Size: 6592 MfeI C'AATT_G Cuts at: 4191 4191 Size: 6592 MluI A'CGCG_T Cuts at: 3232 6468 3232 Size: 3236 3356 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2634 3495 3627 5706 5890 1305 Size: 1329 861 132 2079 184 2007 Fragments arranged by size: 2079 2007 1329 861 184 132 MscI TGG'CCA Cuts at: 10 64 1489 1832 2481 2646 10 Size: 54 1425 343 649 165 3956 Fragments arranged by size: 3956 1425 649 343 165 54 MslI CAynn'nnrTG Cuts at: 518 1166 2754 3635 3764 4550 4909 5068 518 Size: 648 1588 881 129 786 359 159 2042 Fragments arranged by size: 2042 1588 881 786 648 359 159 129 MspA1I CmG'CkG Cuts at: 1606 2374 2815 3141 3163 3171 4008 4685 5833 Size: 768 441 326 22 8 837 677 1148 Cuts at: 5833 6078 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 837 768 677 441 326 245 22 8 NarI GG'CG_CC Cuts at: 3111 3111 Size: 6592 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6040 NdeI CA'TA_TG Cuts at: 387 387 Size: 6592 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6592 NotI GC'GGCC_GC Cuts at: 4039 4039 Size: 6592 NruI TCG'CGA Cuts at: 2013 2013 Size: 6592 NspI r_CATG'y Cuts at: 1132 1229 3694 3763 3950 5495 1132 Size: 97 2465 69 187 1545 2229 Fragments arranged by size: 2465 2229 1545 187 97 69 PasI CC'CwG_GG Cuts at: 1348 3859 1348 Size: 2511 4081 PciI A'CATG_T Cuts at: 5491 5491 Size: 6592 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6592 PfoI T'CCnGG_A Cuts at: 2949 2949 Size: 6592 PmeI GTTT'AAAC Cuts at: 3896 3896 Size: 6592 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4661 4693 6174 6206 4661 Size: 32 1481 32 5047 Fragments arranged by size: 5047 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3211 3723 3211 Size: 512 6080 PshAI GACnn'nnGTC Cuts at: 1613 3318 1613 Size: 1705 4887 PsiI TTA'TAA Cuts at: 4162 4162 Size: 6592 PspOMI G'GGCC_C Cuts at: 3061 3061 Size: 6592 PstI C_TGCA'G Cuts at: 830 3944 830 Size: 3114 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6592 PvuII CAG'CTG Cuts at: 2374 2815 3163 3171 2374 Size: 441 348 8 5795 Fragments arranged by size: 5795 441 348 8 SalI G'TCGA_C Cuts at: 1936 2464 3934 1936 Size: 528 1470 4594 Fragments arranged by size: 4594 1470 528 SbfI CC_TGCA'GG Cuts at: 3944 3944 Size: 6592 ScaI AGT'ACT Cuts at: 4840 6552 4840 Size: 1712 4880 SexAI A'CCwGG_T Cuts at: 2016 2016 Size: 6592 SfcI C'TryA_G Cuts at: 826 1045 2989 3940 5075 5756 5947 826 Size: 219 1944 951 1135 681 191 1471 Fragments arranged by size: 1944 1471 1135 951 681 219 191 SfoI GGC'GCC Cuts at: 3112 3112 Size: 6592 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6592 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6592 SmaI CCC'GGG Cuts at: 1872 3220 3676 3919 1872 Size: 1348 456 243 4545 Fragments arranged by size: 4545 1348 456 243 SmlI C'TyrA_G Cuts at: 820 1017 1943 2090 4696 5597 5859 820 Size: 197 926 147 2606 901 262 1553 Fragments arranged by size: 2606 1553 926 901 262 197 147 SnaBI TAC'GTA Cuts at: 493 4383 493 Size: 3890 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6592 SphI G_CATG'C Cuts at: 1132 3694 3950 1132 Size: 2562 256 3774 Fragments arranged by size: 3774 2562 256 SspI AAT'ATT Cuts at: 5 52 4516 5 Size: 47 4464 2081 Fragments arranged by size: 4464 2081 47 StuI AGG'CCT Cuts at: 1517 2987 1517 Size: 1470 5122 StyI C'CwwG_G Cuts at: 513 1065 2226 4031 513 Size: 552 1161 1805 3074 Fragments arranged by size: 3074 1805 1161 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2556 3387 3719 3815 3863 4619 4636 Size: 1255 976 831 332 96 48 756 17 Cuts at: 4636 4789 4974 5419 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 976 831 756 445 332 185 153 96 48 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2711 4838 6550 Size: 274 80 33 51 1443 734 2127 1712 Cuts at: 6550 96 Size: 138 Fragments arranged by size: 2127 1712 1443 734 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3818 3850 6552 6584 3818 Size: 32 2702 32 3826 Fragments arranged by size: 3826 2702 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2018 1316 Size: 702 5890 XbaI T'CTAG_A Cuts at: 3421 3928 3421 Size: 507 6085 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6592 XmaI C'CCGG_G Cuts at: 1870 3218 3674 3917 1870 Size: 1348 456 243 4545 Fragments arranged by size: 4545 1348 456 243 XmnI GAAnn'nnTTC Cuts at: 4721 4721 Size: 6592 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4400 276 Size: 53 83 186 936 2866 2468 Fragments arranged by size: 2866 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI FalI FseI FspAI NaeI NgoMIV NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI XcmI