Restriction map for pFN21AE6646
 (Circular) (Six-base) MAPSORT of: pFN21AE6646.seq  Check: 8529  from: 1  to: 5383



pFN21AE6646  5383 bp

 With 182 enzymes: SgfI * 

                        March 18, 2015 14:53  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1999    2354    1999 
   Size:        355    5028
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3193     278 
   Size:         53      83     186     936    1657    2468

  Fragments arranged by size: 

                2468    1657     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2726    1937 
   Size:        789    4594
 
Acc65I G'GTAC_C

Cuts at:   2619    2704    2619 
   Size:         85    5298
 
AclI AA'CG_TT

Cuts at:   1563    3510    3883    1563 
   Size:       1947     373    3063

  Fragments arranged by size: 

                3063    1947     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2684    3446    4830    5098    1632 
   Size:       1052     762    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1052     762     268
 
AfeI AGC'GCT

Cuts at:   2596    2596 
   Size:       5383
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5186
 
AflIII A'CryG_T

Cuts at:   1203    4282    5259    1203 
   Size:       3079     977    1327

  Fragments arranged by size: 

                3079    1327     977
 
AgeI A'CCGG_T

Cuts at:   4214    4214 
   Size:       5383
 
AhdI GACnn_n'nnGTC

Cuts at:   4112    4112 
   Size:       5383
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5383
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5383
 
AlwNI CAG_nnn'CTG

Cuts at:   3126    4698    3126 
   Size:       1572    3811
 
ApaLI G'TGCA_C

Cuts at:   3440    4596    3440 
   Size:       1156    4227
 
ApoI r'AATT_y

Cuts at:   1493    2443    2692    2918    3070    5179    1493 
   Size:        950     249     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     950     249     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3937     160 
   Size:        872    2905    1606

  Fragments arranged by size: 

                2905    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5383
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2708    1870 
   Size:         73     765    4545

  Fragments arranged by size: 

                4545     765      73
 
BamHI G'GATC_C

Cuts at:   2713    3080    2713 
   Size:        367    5016
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2318    2619    2704    4159     618 
   Size:        325     217     627     531     301      85    1455    1842

  Fragments arranged by size: 

                1842    1455     627     531     325     301     217      85
            
 
BanII G_rGCy'C

Cuts at:   1391    2702    1391 
   Size:       1311    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4412
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    3574    3608    1903 
   Size:         34      50      34    1553      34    3678

  Fragments arranged by size: 

                3678    1553      50      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3275    4485    3275 
   Size:       1210    4173
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5383
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3994     136 
   Size:        107     122      71     631     605    2322    1525

  Fragments arranged by size: 

                2322    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5378    5378 
   Size:       5383
 
BlpI GC'TnA_GC

Cuts at:   2800    2800 
   Size:       5383
 
Bme1580I G_kGCm'C

Cuts at:   1790    3444    4600    1790 
   Size:       1654    1156    2573

  Fragments arranged by size: 

                2573    1654    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5383
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2118    2569    3114 
   Size:        416      53      83     399      24     701     451     545

Cuts at:   3114    4072     442 
   Size:        958    1753

  Fragments arranged by size: 

                1753     958     701     545     451     416     399      83
                  53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5383
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2568    2568 
   Size:       5383
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2361    4043    1239 
   Size:       1122    1682    2579

  Fragments arranged by size: 

                2579    1682    1122
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3508    4373    4671    3508 
   Size:        865     298    4220

  Fragments arranged by size: 

                4220     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4046     882 
   Size:        617    2547    2219

  Fragments arranged by size: 

                2547    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3174     493 
   Size:        711    1970    2702

  Fragments arranged by size: 

                2702    1970     711
 
BsaBI GATnn'nnATC

Cuts at:   5377    5377 
   Size:       5383
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3190    3572    4207     275 
   Size:         53      83     186     936    1657     382     635    1451

  Fragments arranged by size: 

                1657    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3816    4214    4488    4635    5046    3816 
   Size:        398     274     147     411    4153

  Fragments arranged by size: 

                4153     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5158

  Fragments arranged by size: 

                5158     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5383
 
BseYI C'CCAG_C

Cuts at:   2213    4586    5082    2213 
   Size:       2373     496    2514

  Fragments arranged by size: 

                2514    2373     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2382    1163 
   Size:        573     646    4164

  Fragments arranged by size: 

                4164     646     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2833    3594    4622    1540 
   Size:        275     186     832     761    1028    2301

  Fragments arranged by size: 

                2301    1028     832     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2493    3444    3529    4600    2493 
   Size:        951      85    1071    3276

  Fragments arranged by size: 

                3276    1071     951      85
 
BsmI GAATG_Cn'

Cuts at:   2231    2674    2894    2987    2231 
   Size:        443     220      93    4627

  Fragments arranged by size: 

                4627     443     220      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2493    2702    3444    3529    4600    1391 
   Size:        399     703     209     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     703     399     209      85
 
BspEI T'CCGG_A

Cuts at:   5046    5046 
   Size:       5383
 
BspHI T'CATG_A

Cuts at:   3272    5002    3272 
   Size:       1730    3653
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    1999    2354    2738     844 
   Size:       1155     355     384    3489

  Fragments arranged by size: 

                3489    1155     384     355
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3270    1394 
   Size:        429    1447    3507

  Fragments arranged by size: 

                3507    1447     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3878    4052      65 
   Size:       1183    2630     174    1396

  Fragments arranged by size: 

                2630    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4027    4214    1700 
   Size:        110    2217     187    2869

  Fragments arranged by size: 

                2869    2217     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5383
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5383
 
BssSI C'ACGA_G

Cuts at:   3443    4455    3443 
   Size:       1012    4371
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5383
 
BstBI TT'CG_AA

Cuts at:   4187    4187 
   Size:       5383
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5383
 
BstYI r'GATC_y

Cuts at:   1972    2456    2713    3080    3465    3482    4934    5020    5032 
   Size:        484     257     367     385      17    1452      86      12

Cuts at:   5032    5378    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     484     385     367     346     257      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    4831
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4644

  Fragments arranged by size: 

                4644     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2236    2344    2899    3693    3713    2236 
   Size:        108     555     794      20    3906

  Fragments arranged by size: 

                3906     794     555     108      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5383
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5348
 
DraI TTT'AAA

Cuts at:   2687    2687 
   Size:       5383
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4390     809 
   Size:        507    3074    1802

  Fragments arranged by size: 

                3074    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2103    2419    2830 
   Size:         54    1425      50     275      18     273     316     411

Cuts at:   2830    3719    5348       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     411     316     275     273      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2830    1537 
   Size:        275    1018    4090

  Fragments arranged by size: 

                4090    1018     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2417    3313    1353 
   Size:       1064     896    3423

  Fragments arranged by size: 

                3423    1064     896
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3970    4344    4490     387 
   Size:       1064     138    2381     374     146    1280

  Fragments arranged by size: 

                2381    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2361    2684    3446    4043    4830    5098    1239 
   Size:        393     729     323     762     597     787     268    1524

  Fragments arranged by size: 

                1524     787     762     729     597     393     323     268
            
 
EcoRI G'AATT_C

Cuts at:   2692    3070    2692 
   Size:        378    5005
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2428    2428 
   Size:       5383
 
FspI TGC'GCA

Cuts at:   3889    3889 
   Size:       5383
 
HaeII r_GCGC'y

Cuts at:   1678    2598    4530    1678 
   Size:        920    1932    2531

  Fragments arranged by size: 

                2531    1932     920
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2448    2480    4024    4056    4098 
   Size:         32     135      32     876      32    1544      32      42

Cuts at:   4098    4130    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1544     876     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2284    2631    2727    2973     669 
   Size:        988     281     346     347      96     246    3079

  Fragments arranged by size: 

                3079     988     347     346     281     246      96
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5383
 
HpaI GTT'AAC

Cuts at:   2973    2973 
   Size:       5383
 
KpnI G_GTAC'C

Cuts at:   2623    2708    2623 
   Size:         85    5298
 
MfeI C'AATT_G

Cuts at:   2982    2982 
   Size:       5383
 
MluI A'CGCG_T

Cuts at:   5259    5259 
   Size:       5383
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4497    4681    1305 
   Size:       3192     184    2007

  Fragments arranged by size: 

                3192    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2105    2421      10 
   Size:         54    1425     343     273     316    2972

  Fragments arranged by size: 

                2972    1425     343     316     273      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2009    3341    3700    3859     518 
   Size:        648     843    1332     359     159    2042

  Fragments arranged by size: 

                2042    1332     843     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2799    3476    4624    4869    1606 
   Size:       1193     677    1148     245    2120

  Fragments arranged by size: 

                2120    1193    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4831
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5383
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5383
 
NotI GC'GGCC_GC

Cuts at:   2830    2830 
   Size:       5383
 
NspI r_CATG'y

Cuts at:   1132    1229    2741    4286    1132 
   Size:         97    1512    1545    2229

  Fragments arranged by size: 

                2229    1545    1512      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5383
 
PciI A'CATG_T

Cuts at:   4282    4282 
   Size:       5383
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2133    1108 
   Size:       1025    4358
 
PmeI GTTT'AAAC

Cuts at:   2687    2687 
   Size:       5383
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3452    3484    4965    4997    3452 
   Size:         32    1481      32    3838

  Fragments arranged by size: 

                3838    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5383
 
PsiI TTA'TAA

Cuts at:   2953    2953 
   Size:       5383
 
PstI C_TGCA'G

Cuts at:    830    2735     830 
   Size:       1905    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5383
 
SalI G'TCGA_C

Cuts at:   1936    2725    1936 
   Size:        789    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2417    2417 
   Size:       5383
 
SbfI CC_TGCA'GG

Cuts at:   2735    2735 
   Size:       5383
 
ScaI AGT'ACT

Cuts at:   3631    5343    3631 
   Size:       1712    3671
 
SfcI C'TryA_G

Cuts at:    826    1045    2731    3866    4547    4738     826 
   Size:        219    1686    1135     681     191    1471

  Fragments arranged by size: 

                1686    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5383
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5383
 
SmaI CCC'GGG

Cuts at:   1872    2710    1872 
   Size:        838    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3487    4388    4650     820 
   Size:        197     926    1544     901     262    1553

  Fragments arranged by size: 

                1553    1544     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3174     493 
   Size:       2681    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5383
 
SphI G_CATG'C

Cuts at:   1132    2741    1132 
   Size:       1609    3774
 
SspI AAT'ATT

Cuts at:      5      52    2180    3307       5 
   Size:         47    2128    1127    2081

  Fragments arranged by size: 

                2128    2081    1127      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5383
 
StyI C'CwwG_G

Cuts at:    513    1065    2822     513 
   Size:        552    1757    3074

  Fragments arranged by size: 

                3074    1757     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3410    3427    3580    3765    4210     325 
   Size:       1255    1830      17     153     185     445    1498

  Fragments arranged by size: 

                1830    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2567    3629    5341 
   Size:        274      80      33      51    1443     590    1062    1712

Cuts at:   5341      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1062     590     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5343    5375    5343 
   Size:         32    5351
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2635    1316 
   Size:       1319    4064
 
XbaI T'CTAG_A

Cuts at:   2719    2719 
   Size:       5383
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2385    2385 
   Size:       5383
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5383
 
XmaI C'CCGG_G

Cuts at:   1870    2708    1870 
   Size:        838    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2411    3512    2411 
   Size:       1101    4282
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3191     276 
   Size:         53      83     186     936    1657    2468

  Fragments arranged by size: 

                2468    1657     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI
    PflMI     PmeI     PpiI    PshAI     PsiI     PstI     PvuI     SalI
     SapI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI    BfrBI
   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII  EcoICRI
    EcoNI EcoO109I    EcoRV     FseI    FspAI     KasI     NaeI     NarI
   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI    PpuMI   PspOMI
    PspXI     PsrI    PvuII    RsrII     SacI    SacII    SanDI    SexAI
     SfiI     SfoI     SrfI     SwaI
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