################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII AccI AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I AspEI AspHI AspI AvaI AviII AvrII BalI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII Hin1I HpaI KasI KspI MamI MluI MluNI MroI MscI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 670 GGTACC G^GTACC AfaI 671 GTAC GT^AC AseI 835 ATTAAT AT^TAAT AsnI 835 ATTAAT AT^TAAT Asp718I 670 GGTACC G^GTACC BamHI 714 GGATCC G^GATCC BanI 670 GGTACC G^G[CT][GA]CC BanII 859 GGGCTC G[GA]GC[CT]^C BfaI 752 CTAG C^TAG BglII 633 AGATCT A^GATCT BmyI 859 GGGCTC G[AGT]GC[ACT]^C BsaI 208 GAGACC GGTCTC(1/5) BsaWI 163 TCCGGT [AT]^CCGG[AT] BsgI 678 CTGCAC GTGCAG(16/14) BsmAI 208 GAGAC GTCTC(1/5) Bsp1286I 859 GGGCTC G[AGT]GC[ACT]^C BspMI 438 ACCTGC ACCTGC(4/8) BsrDI 693 CATTGC GCAATG(2/0) BssHII 23 GCGCGC G^CGCGC BstF5I 120 GGATG GGATG(2/0) BstXI 849 CCACAAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 199 GGGACCT [GA]G^G[ACGT]CC[CT] EarI 828 GAAGAG CTCTTC(1/4) EcoO109I 199 GGGACCT [GA]G^G[ACGT]CC[CT] FokI 120 GGATG GGATG(9/13) HapII 164 CCGG C^CGG HgaI 27 GCGTC GACGC(5/10) HindIII 799 AAGCTT A^AGCTT HinfI 780 GACTC G^A[ACGT]TC HpaII 164 CCGG C^CGG KpnI 670 GGTACC GGTAC^C Ksp632I 828 GAAGAG CTCTTC(1/4) MaeI 752 CTAG C^TAG MaeII 160 ACGT A^CGT MaeIII 193 GTTAC ^GT[ACGT]AC MfeI 290 CAATTG C^AATTG MslI 574 CACACTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 455 CAGCTG C[AC]G^C[GT]G MspI 164 CCGG C^CGG MunI 290 CAATTG C^AATTG MwoI 433 GCTGGACCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 447 CCATGG C^CATGG NdeI 50 CATATG CA^TATG NlaIII 448 CATG CATG^ PleI 780 GACTC GAGTC(4/5) PpuMI 199 GGGACCT [GA]G^G[AT]CC[CT] PshBI 835 ATTAAT AT^TAAT PvuII 455 CAGCTG CAG^CTG RsaI 671 GTAC GT^AC SapI 828 GAAGAGC GCTCTTC(1/4) SfcI 549 CTACAG C^T[GA][CT]AG StuI 787 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AccII 24 CGCG CG^CG 26 CGCG AcsI 151 GAATTT [GA]^AATT[CT] 598 GAATTT AlwNI 179 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG 778 CAGACTCTG ApoI 151 GAATTT [GA]^AATT[CT] 598 GAATTT AvaII 200 GGACC G^G[AT]CC 436 GGACC BbsI 295 GAAGAC GAAGAC(2/6) 378 GTCTTC BbvI 697 GCAGC GCAGC(8/12) 70 GCTGC BpmI 842 CTGGAG CTGGAG(16/14) 868 CTGGAG BpuAI 295 GAAGAC GAAGAC(2/6) 378 GTCTTC BsiYI 346 CCAAACACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 867 CCTGGAGTTGG BslI 346 CCAAACACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 867 CCTGGAGTTGG BssKI 790 CCTGG ^CC[ACGT]GG 867 CCTGG BstNI 790 CCTGG CC^[AT]GG 867 CCTGG BstUI 24 CGCG CG^CG 26 CGCG BstYI 633 AGATCT [GA]^GATC[CT] 714 GGATCC CfoI 23 GCGC GCG^C 25 GCGC Cfr13I 200 GGACC G^G[ACGT]CC 436 GGACC DdeI 776 CTCAG C^T[ACGT]AG 784 CTGAG DsaI 9 CCACGG C^C[GA][CT]GG 447 CCATGG EcoRII 790 CCTGG ^CC[AT]GG 867 CCTGG Fnu4HI 70 GCTGC GC^[ACGT]GC 697 GCAGC HaeIII 788 GGCC GG^CC 847 GGCC HhaI 23 GCGC GCG^C 25 GCGC HinP1I 23 GCGC G^CGC 25 GCGC HincII 418 GTTGAC GT[CT]^[GA]AC 742 GTCAAC HindII 418 GTTGAC GT[CT]^[GA]AC 742 GTCAAC HphI 111 GGTGA GGTGA(8/7) 343 TCACC ItaI 70 GCTGC GC^[ACGT]GC 697 GCAGC MflI 633 AGATCT [GA]^GATC[CT] 714 GGATCC MseI 757 TTAA T^TAA 836 TTAA MvaI 790 CCTGG CC^[AT]GG 867 CCTGG MvnI 24 CGCG CG^CG 26 CGCG Sau96I 200 GGACC G^G[ACGT]CC 436 GGACC ScrFI 790 CCTGG CC^[ACGT]GG 867 CCTGG Tru9I 757 TTAA T^TAA 836 TTAA TseI 70 GCTGC G^C[AT]GC 697 GCAGC XhoII 633 AGATCT [GA]^GATC[CT] 714 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 17 enzymes) # ######################################### AluI AlwI BsaJI BsmFI BsrI Cac8I DpnII EcoT14I MboI MboII MnlI NdeII NlaIV Sau3AI StyI Tsp509I TspRI