(Circular) (Six-base) MAPSORT of: pFN21AE5355.seq Check: 3083 from: 1 to: 5587 pFN21AE5355 5587 bp With 182 enzymes: SgfI * January 30, 2014 17:18 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3397 278 Size: 53 83 186 936 1861 2468 Fragments arranged by size: 2468 1861 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2930 1937 Size: 993 4594 Acc65I G'GTAC_C Cuts at: 2675 2908 2675 Size: 233 5354 AclI AA'CG_TT Cuts at: 1563 3714 4087 1563 Size: 2151 373 3063 Fragments arranged by size: 3063 2151 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2634 3650 5034 5302 1632 Size: 1002 1016 1384 268 1917 Fragments arranged by size: 1917 1384 1016 1002 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5390 AflIII A'CryG_T Cuts at: 1203 4486 5463 1203 Size: 3283 977 1327 Fragments arranged by size: 3283 1327 977 AgeI A'CCGG_T Cuts at: 4418 4418 Size: 5587 AhdI GACnn_n'nnGTC Cuts at: 4316 4316 Size: 5587 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5587 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5587 AlwNI CAG_nnn'CTG Cuts at: 2189 2788 3330 4902 2189 Size: 599 542 1572 2874 Fragments arranged by size: 2874 1572 599 542 ApaLI G'TGCA_C Cuts at: 3644 4800 3644 Size: 1156 4431 ApoI r'AATT_y Cuts at: 1493 2156 2603 2896 3122 3274 5383 1493 Size: 663 447 293 226 152 2109 1697 Fragments arranged by size: 2109 1697 663 447 293 226 152 AseI AT'TA_AT Cuts at: 160 1032 2841 4141 160 Size: 872 1809 1300 1606 Fragments arranged by size: 1809 1606 1300 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5587 AvaI C'yCGr_G Cuts at: 1870 1943 2912 1870 Size: 73 969 4545 Fragments arranged by size: 4545 969 73 BamHI G'GATC_C Cuts at: 2719 2917 3284 2719 Size: 198 367 5022 Fragments arranged by size: 5022 367 198 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2675 2908 4363 618 Size: 325 217 627 888 233 1455 1842 Fragments arranged by size: 1842 1455 888 627 325 233 217 BanII G_rGCy'C Cuts at: 1391 2868 2906 1391 Size: 1477 38 4072 Fragments arranged by size: 4072 1477 38 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2307 2376 928 Size: 971 408 69 4139 Fragments arranged by size: 4139 971 408 69 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3778 3812 1903 Size: 34 1841 34 3678 Fragments arranged by size: 3678 1841 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3479 4689 3479 Size: 1210 4377 BclI T'GATC_A Cuts at: 1548 1548 Size: 5587 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4198 136 Size: 107 122 71 631 605 2526 1525 Fragments arranged by size: 2526 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2638 5582 2638 Size: 2944 2643 BlpI GC'TnA_GC Cuts at: 3004 3004 Size: 5587 Bme1580I G_kGCm'C Cuts at: 1790 3648 4804 1790 Size: 1858 1156 2573 Fragments arranged by size: 2573 1858 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5587 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2211 2571 3318 Size: 416 53 83 399 24 794 360 747 Cuts at: 3318 4276 442 Size: 958 1753 Fragments arranged by size: 1753 958 794 747 416 399 360 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5587 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2868 2894 4247 1239 Size: 1629 26 1353 2579 Fragments arranged by size: 2579 1629 1353 26 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3712 4577 4875 3712 Size: 865 298 4424 Fragments arranged by size: 4424 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2207 4250 882 Size: 617 708 2043 2219 Fragments arranged by size: 2219 2043 708 617 BsaAI yAC'GTr Cuts at: 493 1204 3378 493 Size: 711 2174 2702 Fragments arranged by size: 2702 2174 711 BsaBI GATnn'nnATC Cuts at: 5581 5581 Size: 5587 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3394 3776 4411 275 Size: 53 83 186 936 1861 382 635 1451 Fragments arranged by size: 1861 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2168 4020 4418 4692 4839 5250 2168 Size: 1852 398 274 147 411 2505 Fragments arranged by size: 2505 1852 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5362 Fragments arranged by size: 5362 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5587 BseYI C'CCAG_C Cuts at: 4790 5286 4790 Size: 496 5091 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2668 1163 Size: 573 932 4082 Fragments arranged by size: 4082 932 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3037 3798 4826 1540 Size: 275 186 1036 761 1028 2301 Fragments arranged by size: 2301 1036 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3648 3733 4804 3648 Size: 85 1071 4431 Fragments arranged by size: 4431 1071 85 BsmI GAATG_Cn' Cuts at: 3098 3191 3098 Size: 93 5494 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2868 2906 3648 3733 4804 1391 Size: 399 1078 38 742 85 1071 2174 Fragments arranged by size: 2174 1078 1071 742 399 85 38 BspEI T'CCGG_A Cuts at: 5250 5250 Size: 5587 BspHI T'CATG_A Cuts at: 3476 5206 3476 Size: 1730 3857 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2452 2942 844 Size: 1608 490 3489 Fragments arranged by size: 3489 1608 490 BsrBI CCG'CTC Cuts at: 1394 1823 3474 1394 Size: 429 1651 3507 Fragments arranged by size: 3507 1651 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2697 4082 4256 65 Size: 1183 1449 1385 174 1396 Fragments arranged by size: 1449 1396 1385 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4231 4418 1700 Size: 110 2421 187 2869 Fragments arranged by size: 2869 2421 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5587 BssHII G'CGCG_C Cuts at: 1925 2028 1925 Size: 103 5484 BssSI C'ACGA_G Cuts at: 3647 4659 3647 Size: 1012 4575 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5587 BstBI TT'CG_AA Cuts at: 4391 4391 Size: 5587 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5587 BstXI CCAn_nnnn'nTGG Cuts at: 2861 2861 Size: 5587 BstYI r'GATC_y Cuts at: 1972 2638 2719 2917 3284 3669 3686 5138 5224 Size: 666 81 198 367 385 17 1452 86 Cuts at: 5224 5236 5582 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 666 385 367 346 198 86 81 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2014 2452 513 Size: 552 939 10 438 3648 Fragments arranged by size: 3648 939 552 438 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4848 Fragments arranged by size: 4848 683 56 BtsI GCAGTG_nn' Cuts at: 2152 2553 2724 3103 3897 3917 2152 Size: 401 171 379 794 20 3822 Fragments arranged by size: 3822 794 401 379 171 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5587 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5552 DraI TTT'AAA Cuts at: 2891 2891 Size: 5587 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4594 809 Size: 507 3278 1802 Fragments arranged by size: 3278 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3034 3923 5552 Size: 54 1425 50 275 18 1204 889 1629 Cuts at: 5552 8 Size: 43 Fragments arranged by size: 1629 1425 1204 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3034 1537 Size: 275 1222 4090 Fragments arranged by size: 4090 1222 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2828 3517 1353 Size: 1475 689 3423 Fragments arranged by size: 3423 1475 689 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4174 4548 4694 387 Size: 1064 138 2585 374 146 1280 Fragments arranged by size: 2585 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2634 2868 2894 3650 4247 5034 5302 Size: 393 1002 234 26 756 597 787 268 Cuts at: 5302 1239 Size: 1524 Fragments arranged by size: 1524 1002 787 756 597 393 268 234 26 EcoO109I rG'GnC_Cy Cuts at: 2205 2205 Size: 5587 EcoRI G'AATT_C Cuts at: 2896 3274 2896 Size: 378 5209 FspI TGC'GCA Cuts at: 4093 4093 Size: 5587 HaeII r_GCGC'y Cuts at: 1678 4734 1678 Size: 3056 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2433 2465 4228 4260 4302 Size: 32 135 32 861 32 1763 32 42 Cuts at: 4302 4334 1373 Size: 32 2626 Fragments arranged by size: 2626 1763 861 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2425 2749 2931 3177 669 Size: 988 281 487 324 182 246 3079 Fragments arranged by size: 3079 988 487 324 281 246 182 HindIII A'AGCT_T Cuts at: 748 2804 748 Size: 2056 3531 HpaI GTT'AAC Cuts at: 3177 3177 Size: 5587 KpnI G_GTAC'C Cuts at: 2679 2912 2679 Size: 233 5354 MfeI C'AATT_G Cuts at: 2295 3186 2295 Size: 891 4696 MluI A'CGCG_T Cuts at: 5463 5463 Size: 5587 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2697 2859 4701 4885 1305 Size: 1392 162 1842 184 2007 Fragments arranged by size: 2007 1842 1392 184 162 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3765 Fragments arranged by size: 3765 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2583 3545 3904 4063 518 Size: 648 1417 962 359 159 2042 Fragments arranged by size: 2042 1417 962 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2462 3003 3680 4828 5073 1606 Size: 856 541 677 1148 245 2120 Fragments arranged by size: 2120 1148 856 677 541 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2452 513 Size: 552 939 448 3648 Fragments arranged by size: 3648 939 552 448 NdeI CA'TA_TG Cuts at: 387 2056 387 Size: 1669 3918 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5587 NotI GC'GGCC_GC Cuts at: 3034 3034 Size: 5587 NspI r_CATG'y Cuts at: 1132 1229 2945 4490 1132 Size: 97 1716 1545 2229 Fragments arranged by size: 2229 1716 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5587 PciI A'CATG_T Cuts at: 4486 4486 Size: 5587 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5587 PmeI GTTT'AAAC Cuts at: 2891 2891 Size: 5587 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3656 3688 5169 5201 3656 Size: 32 1481 32 4042 Fragments arranged by size: 4042 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2205 2205 Size: 5587 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5587 PsiI TTA'TAA Cuts at: 3157 3157 Size: 5587 PstI C_TGCA'G Cuts at: 830 2939 830 Size: 2109 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5587 PvuII CAG'CTG Cuts at: 2462 2462 Size: 5587 SalI G'TCGA_C Cuts at: 1936 2929 1936 Size: 993 4594 SapI GCTCTTCn'nnn_ Cuts at: 2828 2828 Size: 5587 SbfI CC_TGCA'GG Cuts at: 2939 2939 Size: 5587 ScaI AGT'ACT Cuts at: 3835 5547 3835 Size: 1712 3875 SfcI C'TryA_G Cuts at: 826 1045 2554 2935 4070 4751 4942 826 Size: 219 1509 381 1135 681 191 1471 Fragments arranged by size: 1509 1471 1135 681 381 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5587 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5587 SmaI CCC'GGG Cuts at: 1872 2914 1872 Size: 1042 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3691 4592 4854 820 Size: 197 926 1748 901 262 1553 Fragments arranged by size: 1748 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3378 493 Size: 2885 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5587 SphI G_CATG'C Cuts at: 1132 2945 1132 Size: 1813 3774 SspI AAT'ATT Cuts at: 5 52 3511 5 Size: 47 3459 2081 Fragments arranged by size: 3459 2081 47 StuI AGG'CCT Cuts at: 1517 2794 1517 Size: 1277 4310 StyI C'CwwG_G Cuts at: 513 1065 2004 2065 2208 2410 2452 3026 513 Size: 552 939 61 143 202 42 574 3074 Fragments arranged by size: 3074 939 574 552 202 143 61 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3614 3631 3784 3969 4414 325 Size: 1255 2034 17 153 185 445 1498 Fragments arranged by size: 2034 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3833 5545 96 Size: 274 80 33 51 1443 1856 1712 138 Fragments arranged by size: 1856 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5547 5579 5547 Size: 32 5555 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5587 XbaI T'CTAG_A Cuts at: 2923 2923 Size: 5587 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5587 XmaI C'CCGG_G Cuts at: 1870 2912 1870 Size: 1042 4545 XmnI GAAnn'nnTTC Cuts at: 3716 3716 Size: 5587 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3395 276 Size: 53 83 186 936 1861 2468 Fragments arranged by size: 2468 1861 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI