################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BciVI BclI BfaI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiWI BsmBI BsmFI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrDI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI Eam1105I EarI Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HindIII HinfI HpaI KpnI Ksp632I KspI MaeI MamI MfeI MluI MluNI MroI MscI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuII RcaI RsrII SacII SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 763 GTCGAC GT^[AC][GT]AC AcyI 850 GGCGCC G[GA]^CG[CT]C Asp700I 777 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 877 GAGCTC G[AT]GC[AT]^C BanII 877 GAGCTC G[GA]GC[CT]^C BbeI 850 GGCGCC GGCGC^C BcgI 125 GCAAGCAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaHI 850 GGCGCC G[GA]^CG[CT]C BsgI 541 GTGCAG GTGCAG(16/14) BsiHKAI 877 GAGCTC G[AT]GC[AT]^C Bsp1407I 749 TGTACA T^GTACA BspMI 108 GCAGGT ACCTGC(4/8) BsrBI 88 GAGCGG CCGCTC(-3/-3) BsrGI 749 TGTACA T^GTACA BstAPI 192 GCAGGGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraII 512 GGGGCCT [GA]G^G[ACGT]CC[CT] DraIII 625 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 166 GACTGCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 512 GGGGCCT [GA]G^G[ACGT]CC[CT] HaeII 850 GGCGCC [GA]GCGC^[CT] Hin1I 850 GGCGCC G[GA]^CG[CT]C HincII 763 GTCGAC GT[CT]^[GA]AC HindII 763 GTCGAC GT[CT]^[GA]AC KasI 850 GGCGCC G^GCGCC MaeIII 658 GTGAC ^GT[ACGT]AC NarI 850 GGCGCC GG^CGCC NspI 599 GCATGC [GA]CATG^[CT] PstI 106 CTGCAG CTGCA^G PvuI 83 CGATCG CGAT^CG SacI 877 GAGCTC GAGCT^C SalI 763 GTCGAC G^TCGAC SfcI 106 CTGCAG C^T[GA][CT]AG SfoI 850 GGCGCC GGC^GCC SphI 599 GCATGC GCATG^C SspBI 749 TGTACA T^GTACA Tsp45I 658 GTGAC ^GT[GC]AC TspRI 531 CAGTG CA[GC]TG[ACGT][ACGT]^ XmnI 777 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### BanI 757 GGCACC G^G[CT][GA]CC 850 GGCGCC BglII 290 AGATCT A^GATCT 779 AGATCT BmyI 756 GGGCAC G[AGT]GC[ACT]^C 877 GAGCTC BpmI 210 CTGGAG CTGGAG(16/14) 327 CTGGAG BsmAI 473 GAGAC GTCTC(1/5) 535 GTCTC Bsp1286I 756 GGGCAC G[AGT]GC[ACT]^C 877 GAGCTC BsrFI 367 GCCGGC [GA]^CCGG[CT] 670 GCCGGC BstYI 290 AGATCT [GA]^GATC[CT] 779 AGATCT Cfr10I 367 GCCGGC [GA]^CCGG[CT] 670 GCCGGC DsaI 74 CCGTGG C^C[GA][CT]GG 665 CCGTGG EcoNI 206 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 516 CCTGTACCAGG EcoT14I 185 CCAAGG C^C[AT][AT]GG 593 CCAAGG MflI 290 AGATCT [GA]^GATC[CT] 779 AGATCT MslI 627 CATCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 711 CATGATGATG MspA1I 397 CCGCTG C[AC]G^C[GT]G 425 CAGCGG NaeI 367 GCCGGC GCC^GGC 670 GCCGGC NgoMIV 367 GCCGGC G^CCGGC 670 GCCGGC SmlI 251 CTCAAG C^T[CT][GA]AG 880 CTCAAG StuI 465 AGGCCT AGG^CCT 803 AGGCCT StyI 185 CCAAGG C^C[AT][AT]GG 593 CCAAGG XhoII 290 AGATCT [GA]^GATC[CT] 779 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AccII AciI AfaI AluI AvaII BbvI BcnI BsaJI BsiEI BsiYI BslI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DpnII EaeI EagI EclXI Eco52I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MboI MboII MnlI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TseI TthHB8I XcmI