Restriction map for
pFN21AE6291
(Circular) (Six-base) MAPSORT of: pFN21AE6291.seq Check: 1816 from: 1 to: 5599
pFN21AE6291 5599 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3409 278
Size: 53 83 186 936 1873 2468
Fragments arranged by size:
2468 1873 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2769 2942 1937
Size: 832 173 4594
Fragments arranged by size:
4594 832 173
Acc65I G'GTAC_C
Cuts at: 2920 2920
Size: 5599
AclI AA'CG_TT
Cuts at: 1563 3726 4099 1563
Size: 2163 373 3063
Fragments arranged by size:
3063 2163 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2518 2773 3662 5046 5314 1632
Size: 886 255 889 1384 268 1917
Fragments arranged by size:
1917 1384 889 886 268 255
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5402
AflIII A'CryG_T
Cuts at: 1203 4498 5475 1203
Size: 3295 977 1327
Fragments arranged by size:
3295 1327 977
AgeI A'CCGG_T
Cuts at: 4430 4430
Size: 5599
AhdI GACnn_n'nnGTC
Cuts at: 4328 4328
Size: 5599
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5599
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2738 1184
Size: 1554 4045
AlwNI CAG_nnn'CTG
Cuts at: 3342 4914 3342
Size: 1572 4027
ApaLI G'TGCA_C
Cuts at: 3656 4812 3656
Size: 1156 4443
ApoI r'AATT_y
Cuts at: 1493 2908 3134 3286 5395 1493
Size: 1415 226 152 2109 1697
Fragments arranged by size:
2109 1697 1415 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4153 160
Size: 872 3121 1606
Fragments arranged by size:
3121 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5599
AvaI C'yCGr_G
Cuts at: 1870 1943 2924 1870
Size: 73 981 4545
Fragments arranged by size:
4545 981 73
BamHI G'GATC_C
Cuts at: 2929 3296 2929
Size: 367 5232
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2762 2855 2920 4375 618
Size: 325 217 627 975 93 65 1455 1842
Fragments arranged by size:
1842 1455 975 627 325 217 93 65
BanII G_rGCy'C
Cuts at: 1391 2886 2918 1391
Size: 1495 32 4072
Fragments arranged by size:
4072 1495 32
BbeI G_GCGC'C
Cuts at: 2859 2859
Size: 5599
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4628
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2119 2153 3790 3824 1903
Size: 34 182 34 1637 34 3678
Fragments arranged by size:
3678 1637 182 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3491 4701 3491
Size: 1210 4389
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5599
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4210 136
Size: 107 122 71 631 605 2538 1525
Fragments arranged by size:
2538 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2295 2784 5594 2295
Size: 489 2810 2300
Fragments arranged by size:
2810 2300 489
BlpI GC'TnA_GC
Cuts at: 3016 3016
Size: 5599
Bme1580I G_kGCm'C
Cuts at: 1790 2765 3660 4816 1790
Size: 975 895 1156 2573
Fragments arranged by size:
2573 1156 975 895
BmgBI CAC'GTC
Cuts at: 1318 2437 1318
Size: 1119 4480
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3330 4288 442
Size: 416 53 83 399 24 1913 958 1753
Fragments arranged by size:
1913 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5599
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2236 2353 4259 1239
Size: 997 117 1906 2579
Fragments arranged by size:
2579 1906 997 117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2241 2870 3724 4589 4887 2241
Size: 629 854 865 298 2953
Fragments arranged by size:
2953 865 854 629 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4262 882
Size: 617 2763 2219
Fragments arranged by size:
2763 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3390 493
Size: 711 2186 2702
Fragments arranged by size:
2702 2186 711
BsaBI GATnn'nnATC
Cuts at: 5593 5593
Size: 5599
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2856 3406 3788 4423
Size: 53 83 186 936 1323 550 382 635
Cuts at: 4423 275
Size: 1451
Fragments arranged by size:
1451 1323 936 635 550 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4032 4430 4704 4851 5262 4032
Size: 398 274 147 411 4369
Fragments arranged by size:
4369 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2342 2372 1178
Size: 30 165 30 939 30 4405
Fragments arranged by size:
4405 939 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5599
BseYI C'CCAG_C
Cuts at: 4802 5298 4802
Size: 496 5103
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2567 1163
Size: 573 831 4195
Fragments arranged by size:
4195 831 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2079 2091 2373 2670 3049 3810
Size: 275 186 78 12 282 297 379 761
Cuts at: 3810 4838 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 379 297 282 275 186
78 12
BsiHKAI G_wGCw'C
Cuts at: 2886 3660 3745 4816 2886
Size: 774 85 1071 3669
Fragments arranged by size:
3669 1071 774 85
BsmI GAATG_Cn'
Cuts at: 3110 3203 3110
Size: 93 5506
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2765 2886 2918 3660 3745 4816 1391
Size: 399 975 121 32 742 85 1071 2174
Fragments arranged by size:
2174 1071 975 742 399 121 85 32
BspEI T'CCGG_A
Cuts at: 5262 5262
Size: 5599
BspHI T'CATG_A
Cuts at: 3488 5218 3488
Size: 1730 3869
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2104 2954 844
Size: 1260 850 3489
Fragments arranged by size:
3489 1260 850
BsrBI CCG'CTC
Cuts at: 1394 1823 2095 3486 1394
Size: 429 272 1391 3507
Fragments arranged by size:
3507 1391 429 272
BsrDI GCAATG_nn'
Cuts at: 65 1248 4094 4268 65
Size: 1183 2846 174 1396
Fragments arranged by size:
2846 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2372 2675 4243 4430 1700
Size: 110 562 303 1568 187 2869
Fragments arranged by size:
2869 1568 562 303 187 110
BsrGI T'GTAC_A
Cuts at: 96 2754 96
Size: 2658 2941
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5599
BssSI C'ACGA_G
Cuts at: 3659 4671 3659
Size: 1012 4587
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2203 794
Size: 1409 4190
BstBI TT'CG_AA
Cuts at: 4403 4403
Size: 5599
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5599
BstYI r'GATC_y
Cuts at: 1972 2295 2784 2929 3296 3681 3698 5150 5236
Size: 323 489 145 367 385 17 1452 86
Cuts at: 5236 5248 5594 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 489 385 367 346 323 145
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2079 2670 513
Size: 552 1014 591 3442
Fragments arranged by size:
3442 1014 591 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2038 487
Size: 683 56 812 4048
Fragments arranged by size:
4048 812 683 56
BtsI GCAGTG_nn'
Cuts at: 3115 3909 3929 3115
Size: 794 20 4785
Fragments arranged by size:
4785 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5599
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5564
DraI TTT'AAA
Cuts at: 2903 2903
Size: 5599
DraIII CAC_nnn'GTG
Cuts at: 2635 2635
Size: 5599
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2177 4606 809
Size: 507 861 2429 1802
Fragments arranged by size:
2429 1802 861 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2076 2370
Size: 54 1425 50 275 18 213 33 294
Cuts at: 2370 2595 2667 2673 3046 3935 5564 8
Size: 225 72 6 373 889 1629 43
Fragments arranged by size:
1629 1425 889 373 294 275 225 213
72 54 50 43 33 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2076 2370 2667 3046 1537
Size: 275 264 294 297 379 4090
Fragments arranged by size:
4090 379 297 294 275 264
EarI CTCTTCn'nnn_
Cuts at: 1353 3529 1353
Size: 2176 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4186 4560 4706 387
Size: 1064 138 2597 374 146 1280
Fragments arranged by size:
2597 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2884 2884
Size: 5599
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2236 2353 2518 2773 3662 4259 5046
Size: 393 604 117 165 255 889 597 787
Cuts at: 5046 5314 1239
Size: 268 1524
Fragments arranged by size:
1524 889 787 604 597 393 268 255
165 117
EcoNI CCTnn'n_nnAGG
Cuts at: 2215 2525 2215
Size: 310 5289
EcoO109I rG'GnC_Cy
Cuts at: 2518 2518
Size: 5599
EcoRI G'AATT_C
Cuts at: 2908 3286 2908
Size: 378 5221
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2314 2830 2314
Size: 516 5083
FspI TGC'GCA
Cuts at: 4105 4105
Size: 5599
HaeII r_GCGC'y
Cuts at: 1678 2859 4746 1678
Size: 1181 1887 2531
Fragments arranged by size:
2531 1887 1181
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2733 2765 4240 4272 4314
Size: 32 135 32 1161 32 1475 32 42
Cuts at: 4314 4346 1373
Size: 32 2626
Fragments arranged by size:
2626 1475 1161 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2770 2943 3189 669
Size: 988 281 832 173 246 3079
Fragments arranged by size:
3079 988 832 281 246 173
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5599
HpaI GTT'AAC
Cuts at: 3189 3189
Size: 5599
KasI G'GCGC_C
Cuts at: 2855 2855
Size: 5599
KpnI G_GTAC'C
Cuts at: 2924 2924
Size: 5599
MfeI C'AATT_G
Cuts at: 3198 3198
Size: 5599
MluI A'CGCG_T
Cuts at: 5475 5475
Size: 5599
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2529 2756 2856 4713 4897 1305
Size: 1224 227 100 1857 184 2007
Fragments arranged by size:
2007 1857 1224 227 184 100
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3777
Fragments arranged by size:
3777 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2636 2720 3557 3916 4075 518
Size: 648 1470 84 837 359 159 2042
Fragments arranged by size:
2042 1470 837 648 359 159 84
MspA1I CmG'CkG
Cuts at: 1606 2404 2432 3015 3692 4840 5085 1606
Size: 798 28 583 677 1148 245 2120
Fragments arranged by size:
2120 1148 798 677 583 245 28
NaeI GCC'GGC
Cuts at: 2374 2677 2374
Size: 303 5296
NarI GG'CG_CC
Cuts at: 2856 2856
Size: 5599
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5047
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5599
NgoMIV G'CCGG_C
Cuts at: 2372 2675 2372
Size: 303 5296
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5599
NotI GC'GGCC_GC
Cuts at: 3046 3046
Size: 5599
NspI r_CATG'y
Cuts at: 1132 1229 2608 2957 4502 1132
Size: 97 1379 349 1545 2229
Fragments arranged by size:
2229 1545 1379 349 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5599
PciI A'CATG_T
Cuts at: 4498 4498
Size: 5599
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5599
PmeI GTTT'AAAC
Cuts at: 2903 2903
Size: 5599
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2447 2479 3668 3700 5181 5213 2447
Size: 32 1189 32 1481 32 2833
Fragments arranged by size:
2833 1481 1189 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5599
PsiI TTA'TAA
Cuts at: 3169 3169
Size: 5599
PstI C_TGCA'G
Cuts at: 830 2115 2951 830
Size: 1285 836 3478
Fragments arranged by size:
3478 1285 836
PvuI CG_AT'CG
Cuts at: 2001 2091 2001
Size: 90 5509
SacI G_AGCT'C
Cuts at: 2886 2886
Size: 5599
SalI G'TCGA_C
Cuts at: 1936 2768 2941 1936
Size: 832 173 4594
Fragments arranged by size:
4594 832 173
SbfI CC_TGCA'GG
Cuts at: 2951 2951
Size: 5599
ScaI AGT'ACT
Cuts at: 3847 5559 3847
Size: 1712 3887
SfcI C'TryA_G
Cuts at: 826 1045 2111 2947 4082 4763 4954 826
Size: 219 1066 836 1135 681 191 1471
Fragments arranged by size:
1471 1135 1066 836 681 219 191
SfoI GGC'GCC
Cuts at: 2857 2857
Size: 5599
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5599
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5599
SmaI CCC'GGG
Cuts at: 1872 2926 1872
Size: 1054 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2256 2885 3703 4604 4866 820
Size: 197 926 313 629 818 901 262 1553
Fragments arranged by size:
1553 926 901 818 629 313 262 197
SnaBI TAC'GTA
Cuts at: 493 3390 493
Size: 2897 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5599
SphI G_CATG'C
Cuts at: 1132 2608 2957 1132
Size: 1476 349 3774
Fragments arranged by size:
3774 1476 349
SspI AAT'ATT
Cuts at: 5 52 3523 5
Size: 47 3471 2081
Fragments arranged by size:
3471 2081 47
StuI AGG'CCT
Cuts at: 1517 2472 2810 1517
Size: 955 338 4306
Fragments arranged by size:
4306 955 338
StyI C'CwwG_G
Cuts at: 513 1065 2190 2598 3038 513
Size: 552 1125 408 440 3074
Fragments arranged by size:
3074 1125 552 440 408
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3626 3643 3796 3981 4426 325
Size: 1255 2046 17 153 185 445 1498
Fragments arranged by size:
2046 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2154 2286 2754
Size: 274 80 33 51 1443 177 132 468
Cuts at: 2754 3845 5557 96
Size: 1091 1712 138
Fragments arranged by size:
1712 1443 1091 468 274 177 138 132
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5559 5591 5559
Size: 32 5567
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5599
XbaI T'CTAG_A
Cuts at: 2935 2935
Size: 5599
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2038 2077 2422 2038
Size: 39 345 5215
Fragments arranged by size:
5215 345 39
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5599
XmaI C'CCGG_G
Cuts at: 1870 2924 1870
Size: 1054 4545
XmnI GAAnn'nnTTC
Cuts at: 2786 3728 2786
Size: 942 4657
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3407 276
Size: 53 83 186 936 1873 2468
Fragments arranged by size:
2468 1873 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SacI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI
SapI SexAI SfiI SrfI SwaI