(Linear) (Six-base) MAPSORT of: KIEE6291.seq Check: 1618 from: 1 to: 894 KIEE6291.orf With 182 enzymes: SgfI * March 16, 2015 11:29 .. AccI GT'mk_AC Cuts at: 0 764 894 Size: 764 130 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 513 768 894 Size: 513 255 126 Fragments arranged by size: 513 255 126 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 733 894 Size: 733 161 BanI G'GyrC_C Cuts at: 0 757 850 894 Size: 757 93 44 Fragments arranged by size: 757 93 44 BanII G_rGCy'C Cuts at: 0 881 894 Size: 881 13 BbeI G_GCGC'C Cuts at: 0 854 894 Size: 854 40 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 114 148 894 Size: 114 34 746 Fragments arranged by size: 746 114 34 BglII A'GATC_T Cuts at: 0 290 779 894 Size: 290 489 115 Fragments arranged by size: 489 290 115 Bme1580I G_kGCm'C Cuts at: 0 760 894 Size: 760 134 BmgBI CAC'GTC Cuts at: 0 432 894 Size: 432 462 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 231 348 894 Size: 231 117 546 Fragments arranged by size: 546 231 117 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 236 865 894 Size: 236 629 29 Fragments arranged by size: 629 236 29 BsaHI Gr'CG_yC Cuts at: 0 851 894 Size: 851 43 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 337 367 894 Size: 337 30 527 Fragments arranged by size: 527 337 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 562 894 Size: 562 332 BsiEI CG_ry'CG Cuts at: 0 74 86 368 665 894 Size: 74 12 282 297 229 Fragments arranged by size: 297 282 229 74 12 BsiHKAI G_wGCw'C Cuts at: 0 881 894 Size: 881 13 Bsp1286I G_dGCh'C Cuts at: 0 760 881 894 Size: 760 121 13 Fragments arranged by size: 760 121 13 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 99 894 Size: 99 795 BsrBI CCG'CTC Cuts at: 0 90 894 Size: 90 804 BsrFI r'CCGG_y Cuts at: 0 367 670 894 Size: 367 303 224 Fragments arranged by size: 367 303 224 BsrGI T'GTAC_A Cuts at: 0 749 894 Size: 749 145 BstAPI GCAn_nnn'nTGC Cuts at: 0 198 894 Size: 198 696 BstYI r'GATC_y Cuts at: 0 290 779 894 Size: 290 489 115 Fragments arranged by size: 489 290 115 BtgI C'CryG_G Cuts at: 0 74 665 894 Size: 74 591 229 Fragments arranged by size: 591 229 74 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 33 894 Size: 33 861 DraIII CAC_nnn'GTG Cuts at: 0 630 894 Size: 630 264 DrdI GACnn_nn'nnGTC Cuts at: 0 172 894 Size: 172 722 EaeI y'GGCC_r Cuts at: 0 38 71 365 590 662 668 894 Size: 38 33 294 225 72 6 226 Fragments arranged by size: 294 226 225 72 38 33 6 EagI C'GGCC_G Cuts at: 0 71 365 662 894 Size: 71 294 297 232 Fragments arranged by size: 297 294 232 71 EcoICRI GAG'CTC Cuts at: 0 879 894 Size: 879 15 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 231 348 513 768 894 Size: 231 117 165 255 126 Fragments arranged by size: 255 231 165 126 117 EcoNI CCTnn'n_nnAGG Cuts at: 0 210 520 894 Size: 210 310 374 Fragments arranged by size: 374 310 210 EcoO109I rG'GnC_Cy Cuts at: 0 513 894 Size: 513 381 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 309 825 894 Size: 309 516 69 Fragments arranged by size: 516 309 69 HaeII r_GCGC'y Cuts at: 0 854 894 Size: 854 40 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 728 760 894 Size: 728 32 134 Fragments arranged by size: 728 134 32 HincII GTy'rAC Cuts at: 0 765 894 Size: 765 129 KasI G'GCGC_C Cuts at: 0 850 894 Size: 850 44 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 524 751 851 894 Size: 524 227 100 43 Fragments arranged by size: 524 227 100 43 MslI CAynn'nnrTG Cuts at: 0 631 715 894 Size: 631 84 179 Fragments arranged by size: 631 179 84 MspA1I CmG'CkG Cuts at: 0 399 427 894 Size: 399 28 467 Fragments arranged by size: 467 399 28 NaeI GCC'GGC Cuts at: 0 369 672 894 Size: 369 303 222 Fragments arranged by size: 369 303 222 NarI GG'CG_CC Cuts at: 0 851 894 Size: 851 43 NgoMIV G'CCGG_C Cuts at: 0 367 670 894 Size: 367 303 224 Fragments arranged by size: 367 303 224 NspI r_CATG'y Cuts at: 0 603 894 Size: 603 291 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 442 474 894 Size: 442 32 420 Fragments arranged by size: 442 420 32 PstI C_TGCA'G Cuts at: 0 110 894 Size: 110 784 PvuI CG_AT'CG Cuts at: 0 86 894 Size: 86 808 SacI G_AGCT'C Cuts at: 0 881 894 Size: 881 13 SalI G'TCGA_C Cuts at: 0 763 894 Size: 763 131 SfcI C'TryA_G Cuts at: 0 106 894 Size: 106 788 SfoI GGC'GCC Cuts at: 0 852 894 Size: 852 42 SmlI C'TyrA_G Cuts at: 0 251 880 894 Size: 251 629 14 Fragments arranged by size: 629 251 14 SphI G_CATG'C Cuts at: 0 603 894 Size: 603 291 StuI AGG'CCT Cuts at: 0 467 805 894 Size: 467 338 89 Fragments arranged by size: 467 338 89 StyI C'CwwG_G Cuts at: 0 185 593 894 Size: 185 408 301 Fragments arranged by size: 408 301 185 TatI w'GTAC_w Cuts at: 0 149 281 749 894 Size: 149 132 468 145 Fragments arranged by size: 468 149 145 132 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 33 72 417 894 Size: 33 39 345 477 Fragments arranged by size: 477 345 39 33 XmnI GAAnn'nnTTC Cuts at: 0 781 894 Size: 781 113 Enzymes that do cut: AccI AcuI AlfI BanI BanII BbeI BcgI BglII Bme1580I BmgBI BpmI BpuEI BsaHI BsaXI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BsrGI BstAPI BstYI BtgI BtgZI DraIII DrdI EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII KasI MmeI MslI MspA1I NaeI NarI NgoMIV NspI PpiI PstI PvuI SacI SalI SfcI SfoI SmlI SphI StuI StyI TatI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BglI BlpI BmrI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaWI BseRI BseYI BsiWI BsmI BsmBI BspEI BspHI BsrDI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraI EarI EciI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MscI NcoI NdeI NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TaqII TstI Tth111I XbaI XhoI XmaI ZraI