################################## # NO CUT ENZYMES ( 189 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AluI Alw44I AlwI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BmyI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Cfr10I Cfr13I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MunI MvnI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AfaI 243 GTAC GT^AC AlwNI 179 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 299 CCTCAGG CC^T[ACGT]AGG BbsI 45 GAAGAC GAAGAC(2/6) BbvCI 289 CCTCAGC CCTCAGC(-5/-2) BlpI 187 GCTGAGC GC^T[ACGT]AGC BpmI 55 CTCCAG CTGGAG(16/14) Bpu1102I 187 GCTGAGC GC^T[ACGT]AGC BpuAI 45 GAAGAC GAAGAC(2/6) BsaAI 240 CACGTA [CT]AC^GT[GA] BseRI 297 CTCCTC GAGGAG(10/8) BspHI 228 TCATGA T^CATGA BspMI 218 ACCTGC ACCTGC(4/8) BsrI 178 CCAGT ACTGG(1/-1) BstF5I 284 GGATG GGATG(2/0) Bsu36I 299 CCTCAGG CC^T[ACGT]AGG CelII 187 GCTGAGC GC^T[ACGT]AGC CfoI 40 GCGC GCG^C EarI 117 GAAGAG CTCTTC(1/4) Eco81I 299 CCTCAGG CC^T[ACGT]AGG EcoRI 141 GAATTC G^AATTC FokI 284 GGATG GGATG(9/13) HaeII 39 GGCGCT [GA]GCGC^[CT] HaeIII 304 GGCC GG^CC HhaI 40 GCGC GCG^C HinP1I 40 GCGC G^CGC HphI 313 TCACC GGTGA(8/7) Ksp632I 117 GAAGAG CTCTTC(1/4) MaeII 241 ACGT A^CGT MaeIII 312 GTCAC ^GT[ACGT]AC MwoI 183 GCCTGCTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIII 229 CATG CATG^ PleI 310 GAGTC GAGTC(4/5) PstI 220 CTGCAG CTGCA^G RcaI 228 TCATGA T^CATGA RsaI 243 GTAC GT^AC TfiI 109 GAATC G^A[AT]TC Tsp45I 312 GTCAC ^GT[GC]AC TspRI 179 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AciI 21 GCGG CCGC(-3/-1) 106 GCGG AcsI 141 GAATTC [GA]^AATT[CT] 277 AAATTC ApoI 141 GAATTC [GA]^AATT[CT] 277 AAATTC BcnI 35 CCGGG CC^[GC]GG 306 CCGGG BsaJI 35 CCGGGG C^C[ACGT][ACGT]GG 65 CCCAGG BsiYI 295 CCCTCCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 299 CCTCAGGCCGG BslI 295 CCCTCCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 299 CCTCAGGCCGG BstNI 57 CCAGG CC^[AT]GG 66 CCAGG Cac8I 171 GCCAGC GC[ACGT]^[ACGT]GC 183 GCCTGC EcoRII 57 CCAGG ^CC[AT]GG 66 CCAGG HapII 35 CCGG C^CGG 306 CCGG HinfI 109 GAATC G^A[ACGT]TC 310 GAGTC HpaII 35 CCGG C^CGG 306 CCGG MboII 45 GAAGA GAAGA(8/7) 117 GAAGA MspI 35 CCGG C^CGG 306 CCGG MvaI 57 CCAGG CC^[AT]GG 66 CCAGG NciI 35 CCGGG CC^[GC]GG 306 CCGGG SfaNI 285 GATGC GCATC(5/9) 225 GCATC SfcI 220 CTGCAG C^T[GA][CT]AG 234 CTACAG Tsp509I 142 AATT ^AATT 278 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 10 enzymes) # ######################################### BbvI BssKI DdeI Fnu4HI ItaI MnlI ScrFI TaqI TseI TthHB8I