(Circular) (Six-base) MAPSORT of: pFN21AE2560.seq Check: 3504 from: 1 to: 5332 pFN21AE2560 5332 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3142 278 Size: 53 83 186 936 1606 2468 Fragments arranged by size: 2468 1606 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2604 2675 1937 Size: 667 71 4594 Fragments arranged by size: 4594 667 71 Acc65I G'GTAC_C Cuts at: 2653 2653 Size: 5332 AclI AA'CG_TT Cuts at: 1563 2269 3459 3832 1563 Size: 706 1190 373 3063 Fragments arranged by size: 3063 1190 706 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2532 2640 3395 4779 5047 1632 Size: 900 108 755 1384 268 1917 Fragments arranged by size: 1917 1384 900 755 268 108 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5135 AflIII A'CryG_T Cuts at: 1203 2197 2356 4231 5208 1203 Size: 994 159 1875 977 1327 Fragments arranged by size: 1875 1327 994 977 159 AgeI A'CCGG_T Cuts at: 4163 4163 Size: 5332 AhdI GACnn_n'nnGTC Cuts at: 4061 4061 Size: 5332 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5332 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5332 AlwNI CAG_nnn'CTG Cuts at: 3075 4647 3075 Size: 1572 3760 ApaLI G'TGCA_C Cuts at: 3389 4545 3389 Size: 1156 4176 ApoI r'AATT_y Cuts at: 1493 2281 2641 2867 3019 5128 1493 Size: 788 360 226 152 2109 1697 Fragments arranged by size: 2109 1697 788 360 226 152 AseI AT'TA_AT Cuts at: 160 1032 3886 160 Size: 872 2854 1606 Fragments arranged by size: 2854 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5332 AvaI C'yCGr_G Cuts at: 1870 1943 2074 2099 2657 1870 Size: 73 131 25 558 4545 Fragments arranged by size: 4545 558 131 73 25 BamHI G'GATC_C Cuts at: 2662 3029 2662 Size: 367 4965 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2053 2653 4108 618 Size: 325 217 627 266 600 1455 1842 Fragments arranged by size: 1842 1455 627 600 325 266 217 BanII G_rGCy'C Cuts at: 1391 2651 1391 Size: 1260 4072 BbeI G_GCGC'C Cuts at: 2057 2057 Size: 5332 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4361 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3523 3557 1903 Size: 34 1586 34 3678 Fragments arranged by size: 3678 1586 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3224 4434 3224 Size: 1210 4122 BclI T'GATC_A Cuts at: 1548 1548 Size: 5332 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3943 136 Size: 107 122 71 631 605 2271 1525 Fragments arranged by size: 2271 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2179 5327 2179 Size: 3148 2184 BlpI GC'TnA_GC Cuts at: 2749 2749 Size: 5332 Bme1580I G_kGCm'C Cuts at: 1790 3393 4549 1790 Size: 1603 1156 2573 Fragments arranged by size: 2573 1603 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5332 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3063 4021 442 Size: 416 53 83 399 24 1646 958 1753 Fragments arranged by size: 1753 1646 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5332 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2031 2392 2031 Size: 361 4971 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3992 1239 Size: 2753 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3457 4322 4620 3457 Size: 865 298 4169 Fragments arranged by size: 4169 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3995 882 Size: 617 2496 2219 Fragments arranged by size: 2496 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2359 3123 493 Size: 711 1155 764 2702 Fragments arranged by size: 2702 1155 764 711 BsaBI GATnn'nnATC Cuts at: 5326 5326 Size: 5332 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2054 3139 3521 4156 Size: 53 83 186 936 521 1085 382 635 Cuts at: 4156 275 Size: 1451 Fragments arranged by size: 1451 1085 936 635 521 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2065 3765 4163 4437 4584 4995 2065 Size: 1700 398 274 147 411 2402 Fragments arranged by size: 2402 1700 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5107 Fragments arranged by size: 5107 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5332 BseYI C'CCAG_C Cuts at: 4535 5031 4535 Size: 496 4836 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4759 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2782 3543 4571 1540 Size: 275 186 781 761 1028 2301 Fragments arranged by size: 2301 1028 781 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3393 3478 4549 3393 Size: 85 1071 4176 Fragments arranged by size: 4176 1071 85 BsmI GAATG_Cn' Cuts at: 2161 2843 2936 2161 Size: 682 93 4557 Fragments arranged by size: 4557 682 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2651 3393 3478 4549 1391 Size: 399 861 742 85 1071 2174 Fragments arranged by size: 2174 1071 861 742 399 85 BspEI T'CCGG_A Cuts at: 2065 4995 2065 Size: 2930 2402 BspHI T'CATG_A Cuts at: 3221 4951 3221 Size: 1730 3602 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2687 844 Size: 1843 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3219 1394 Size: 429 1396 3507 Fragments arranged by size: 3507 1396 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3827 4001 65 Size: 1183 2579 174 1396 Fragments arranged by size: 2579 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3976 4163 1700 Size: 110 2166 187 2869 Fragments arranged by size: 2869 2166 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5332 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5332 BssSI C'ACGA_G Cuts at: 3392 4404 3392 Size: 1012 4320 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5332 BstBI TT'CG_AA Cuts at: 4136 4136 Size: 5332 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5332 BstYI r'GATC_y Cuts at: 1972 2179 2662 3029 3414 3431 4883 4969 4981 Size: 207 483 367 385 17 1452 86 12 Cuts at: 4981 5327 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 483 385 367 346 207 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4780 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4593 Fragments arranged by size: 4593 683 56 BtsI GCAGTG_nn' Cuts at: 2848 3642 3662 2848 Size: 794 20 4518 Fragments arranged by size: 4518 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5332 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5297 DraI TTT'AAA Cuts at: 2242 2565 2636 2242 Size: 323 71 4938 Fragments arranged by size: 4938 323 71 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4339 809 Size: 507 3023 1802 Fragments arranged by size: 3023 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2779 3668 5297 Size: 54 1425 50 275 18 949 889 1629 Cuts at: 5297 8 Size: 43 Fragments arranged by size: 1629 1425 949 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2779 1537 Size: 275 967 4090 Fragments arranged by size: 4090 967 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2574 3262 1353 Size: 1221 688 3423 Fragments arranged by size: 3423 1221 688 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3919 4293 4439 387 Size: 1064 138 2330 374 146 1280 Fragments arranged by size: 2330 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2532 2640 3395 3992 4779 5047 1239 Size: 393 900 108 755 597 787 268 1524 Fragments arranged by size: 1524 900 787 755 597 393 268 108 EcoRI G'AATT_C Cuts at: 2641 3019 2641 Size: 378 4954 EcoRV GAT'ATC Cuts at: 2518 2518 Size: 5332 FspI TGC'GCA Cuts at: 3838 3838 Size: 5332 HaeII r_GCGC'y Cuts at: 1678 2057 4479 1678 Size: 379 2422 2531 Fragments arranged by size: 2531 2422 379 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2123 2155 3973 4005 4047 Size: 32 135 32 551 32 1818 32 42 Cuts at: 4047 4079 1373 Size: 32 2626 Fragments arranged by size: 2626 1818 551 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2676 2922 669 Size: 988 281 738 246 3079 Fragments arranged by size: 3079 988 738 281 246 HindIII A'AGCT_T Cuts at: 748 2105 748 Size: 1357 3975 HpaI GTT'AAC Cuts at: 2922 2922 Size: 5332 KasI G'GCGC_C Cuts at: 2053 2053 Size: 5332 KpnI G_GTAC'C Cuts at: 2657 2657 Size: 5332 MfeI C'AATT_G Cuts at: 2931 2931 Size: 5332 MluI A'CGCG_T Cuts at: 5208 5208 Size: 5332 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4446 4630 1305 Size: 3141 184 2007 Fragments arranged by size: 3141 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3510 Fragments arranged by size: 3510 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3290 3649 3808 518 Size: 648 2124 359 159 2042 Fragments arranged by size: 2124 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2748 3425 4573 4818 1606 Size: 1142 677 1148 245 2120 Fragments arranged by size: 2120 1148 1142 677 245 NarI GG'CG_CC Cuts at: 2054 2054 Size: 5332 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4780 NdeI CA'TA_TG Cuts at: 387 387 Size: 5332 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5332 NotI GC'GGCC_GC Cuts at: 2779 2779 Size: 5332 NspI r_CATG'y Cuts at: 1132 1229 2201 2690 4235 1132 Size: 97 972 489 1545 2229 Fragments arranged by size: 2229 1545 972 489 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5332 PciI A'CATG_T Cuts at: 2197 4231 2197 Size: 2034 3298 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5332 PmeI GTTT'AAAC Cuts at: 2636 2636 Size: 5332 PmlI CAC'GTG Cuts at: 2359 2359 Size: 5332 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3401 3433 4914 4946 3401 Size: 32 1481 32 3787 Fragments arranged by size: 3787 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5332 PsiI TTA'TAA Cuts at: 2414 2505 2902 2414 Size: 91 397 4844 Fragments arranged by size: 4844 397 91 PstI C_TGCA'G Cuts at: 830 2684 830 Size: 1854 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5332 SalI G'TCGA_C Cuts at: 1936 2674 1936 Size: 738 4594 SbfI CC_TGCA'GG Cuts at: 2684 2684 Size: 5332 ScaI AGT'ACT Cuts at: 2263 3580 5292 2263 Size: 1317 1712 2303 Fragments arranged by size: 2303 1712 1317 SfcI C'TryA_G Cuts at: 826 1045 2183 2680 3815 4496 4687 826 Size: 219 1138 497 1135 681 191 1471 Fragments arranged by size: 1471 1138 1135 681 497 219 191 SfoI GGC'GCC Cuts at: 2055 2055 Size: 5332 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5332 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5332 SmaI CCC'GGG Cuts at: 1872 2076 2659 1872 Size: 204 583 4545 Fragments arranged by size: 4545 583 204 SmlI C'TyrA_G Cuts at: 820 1017 1943 2099 3436 4337 4599 820 Size: 197 926 156 1337 901 262 1553 Fragments arranged by size: 1553 1337 926 901 262 197 156 SnaBI TAC'GTA Cuts at: 493 3123 493 Size: 2630 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5332 SphI G_CATG'C Cuts at: 1132 2690 1132 Size: 1558 3774 SspI AAT'ATT Cuts at: 5 52 3256 5 Size: 47 3204 2081 Fragments arranged by size: 3204 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5332 StyI C'CwwG_G Cuts at: 513 1065 2771 513 Size: 552 1706 3074 Fragments arranged by size: 3074 1706 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3359 3376 3529 3714 4159 325 Size: 1255 1779 17 153 185 445 1498 Fragments arranged by size: 1779 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2261 2322 3578 Size: 274 80 33 51 1443 284 61 1256 Cuts at: 3578 5290 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1256 284 274 138 80 61 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5292 5324 5292 Size: 32 5300 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5332 XbaI T'CTAG_A Cuts at: 2668 2668 Size: 5332 XhoI C'TCGA_G Cuts at: 1943 2099 1943 Size: 156 5176 XmaI C'CCGG_G Cuts at: 1870 2074 2657 1870 Size: 204 583 4545 Fragments arranged by size: 4545 583 204 XmnI GAAnn'nnTTC Cuts at: 3461 3461 Size: 5332 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3140 276 Size: 53 83 186 936 1606 2468 Fragments arranged by size: 2468 1606 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI