################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BlnI BsaBI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrFI BsrGI Bst1107I BstEII BstPI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KasI KpnI Ksp632I MaeIII MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SalI SapI ScaI SfoI SgrAI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI TfiI Tsp45I Tsp509I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AcyI 253 GACGCC G[GA]^CG[CT]C AflIII 326 ACACGT A^C[GA][CT]GT AlwI 534 GGATC GGATC(4/5) AocI 234 CCTGAGG CC^T[ACGT]AGG ApaI 54 GGGCCC GGGCC^C AspHI 1135 GAGCTC G[AT]GC[AT]^C AviII 833 TGCGCA TGC^GCA BanI 1041 GGTGCC G^G[CT][GA]CC BbsI 667 GTCTTC GAAGAC(2/6) BfaI 19 CTAG C^TAG BglII 320 AGATCT A^GATCT BpuAI 667 GTCTTC GAAGAC(2/6) BsaAI 559 TACGTG [CT]AC^GT[GA] BsaHI 253 GACGCC G[GA]^CG[CT]C BsaI 571 GAGACC GGTCTC(1/5) BsaWI 422 ACCGGA [AT]^CCGG[AT] BsgI 400 CTGCAC GTGCAG(16/14) BsiHKAI 1135 GAGCTC G[AT]GC[AT]^C BsmAI 571 GAGAC GTCTC(1/5) BspHI 314 TCATGA T^CATGA BspMI 398 ACCTGC ACCTGC(4/8) BsrDI 372 CATTGC GCAATG(2/0) BssHII 639 GCGCGC G^CGCGC BstBI 697 TTCGAA TT^CGAA BstXI 377 CCAAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 320 AGATCT [GA]^GATC[CT] Bsu36I 234 CCTGAGG CC^T[ACGT]AGG DsaI 1100 CCGCGG C^C[GA][CT]GG EaeI 302 TGGCCG [CT]^GGCC[GA] Eco81I 234 CCTGAGG CC^T[ACGT]AGG FspI 833 TGCGCA TGC^GCA HaeII 659 AGCGCC [GA]GCGC^[CT] Hin1I 253 GACGCC G[GA]^CG[CT]C HinfI 1117 GACTC G^A[ACGT]TC KspI 1100 CCGCGG CCGC^GG MaeI 19 CTAG C^TAG MflI 320 AGATCT [GA]^GATC[CT] MseI 464 TTAA T^TAA MslI 1074 CATGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NspV 697 TTCGAA TT^CGAA PflMI 591 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1117 GACTC GAGTC(4/5) PvuII 616 CAGCTG CAG^CTG RcaI 314 TCATGA T^CATGA SacI 1135 GAGCTC GAGCT^C SacII 1100 CCGCGG CCGC^GG SbfI 885 CCTGCAGG CCTGCA^GG SexAI 83 ACCAGGT A^CC[AT]GGT SfaNI 260 GCATC GCATC(5/9) SfiI 1098 GGCCGCGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 697 TTCGAA TT^CGAA SmaI 44 CCCGGG CCC^GGG Sse8387I 885 CCTGCAGG CCTGCA^GG Tru9I 464 TTAA T^TAA Van91I 591 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 290 CCACCGACCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 320 AGATCT [GA]^GATC[CT] XmaCI 44 CCCGGG C^CCGGG XmaI 44 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### BglI 181 GCCCACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1099 GCCGCGGGGGC BlpI 564 GCTGAGC GC^T[ACGT]AGC 780 GCTGAGC Bpu1102I 564 GCTGAGC GC^T[ACGT]AGC 780 GCTGAGC BssSI 484 CACGAG CACGAG(-5/-1) 1065 CTCGTG BstAPI 4 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 461 GCATTAACTGC CelII 564 GCTGAGC GC^T[ACGT]AGC 780 GCTGAGC EcoNI 234 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 882 CCTCCTGCAGG EcoT14I 578 CCTTGG C^C[AT][AT]GG 797 CCAAGG HgaI 253 GACGC GACGC(5/10) 665 GCGTC PpuMI 478 GGGACCC [GA]G^G[AT]CC[CT] 542 AGGACCC StyI 578 CCTTGG C^C[AT][AT]GG 797 CCAAGG TaqI 698 TCGA T^CGA 1151 TCGA TspRI 222 CACTG CA[GC]TG[ACGT][ACGT]^ 299 CAGTG TthHB8I 698 TCGA T^CGA 1151 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI AvaI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiYI BslI BsmFI BsoBI Bsp1286I BsrBI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PstI RsaI Sau3AI Sau96I ScrFI SfcI TseI