Restriction map for
pFN21AE6679
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# NO CUT ENZYMES ( 134 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaLI
AscI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsiYI BslI BsmBI BsmI
Bsp1407I BspEI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstUI BstXI BstYI BstZ17I CelII
Cfr10I CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
Hin1I HindIII HpaI KasI KpnI
KspI MflI MluI MroI MslI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
Psp1406I PvuI RsaI RsrII SacII
SalI ScaI SexAI SfoI SgrAI
SnaBI SpeI SphI SspBI SspI
StuI StyI SwaI Tth111I Van91I
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 277 GTAGAC GT^[AC][GT]AC
AciI 735 GCGG CCGC(-3/-1)
AocI 889 CCTAAGG CC^T[ACGT]AGG
AseI 436 ATTAAT AT^TAAT
AsnI 436 ATTAAT AT^TAAT
AspHI 941 GAGCTC G[AT]GC[AT]^C
BbsI 3 GAAGAC GAAGAC(2/6)
BcgI 499 GCAGCCTTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 896 GCCCCCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 3 GAAGAC GAAGAC(2/6)
BsaBI 711 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 564 GAGGAG GAGGAG(10/8)
BsgI 39 GTGCAG GTGCAG(16/14)
BsiHKAI 941 GAGCTC G[AT]GC[AT]^C
BsmAI 488 GTCTC GTCTC(1/5)
BspDI 862 ATCGAT AT^CGAT
BspHI 548 TCATGA T^CATGA
BsrDI 245 CATTGC GCAATG(2/0)
BstEII 877 GGTGACC G^GT[ACGT]ACC
BstPI 877 GGTGACC G^GT[ACGT]ACC
Bsu36I 889 CCTAAGG CC^T[ACGT]AGG
CfoI 968 GCGC GCG^C
ClaI 862 ATCGAT AT^CGAT
EarI 212 GAAGAG CTCTTC(1/4)
Eco81I 889 CCTAAGG CC^T[ACGT]AGG
EcoO65I 877 GGTGACC G^GT[ACGT]ACC
HaeII 967 GGCGCT [GA]GCGC^[CT]
HgaI 78 GCGTC GACGC(5/10)
HhaI 968 GCGC GCG^C
HinP1I 968 GCGC G^CGC
HincII 586 GTTGAC GT[CT]^[GA]AC
HindII 586 GTTGAC GT[CT]^[GA]AC
Ksp632I 212 GAAGAG CTCTTC(1/4)
MaeII 346 ACGT A^CGT
MamI 711 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 632 CAATTG C^AATTG
MseI 437 TTAA T^TAA
MspA1I 68 CAGCTG C[AC]G^C[GT]G
MunI 632 CAATTG C^AATTG
PshAI 880 GACCGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 436 ATTAAT AT^TAAT
PvuII 68 CAGCTG CAG^CTG
RcaI 548 TCATGA T^CATGA
SacI 941 GAGCTC GAGCT^C
SapI 212 GAAGAGC GCTCTTC(1/4)
SbfI 916 CCTGCAGG CCTGCA^GG
SfiI 895 GGCCCCCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 680 CTCAAG C^T[CT][GA]AG
Sse8387I 916 CCTGCAGG CCTGCA^GG
Tru9I 437 TTAA T^TAA
XbaI 208 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 22 enzymes) #
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AlwI 717 GATCC GGATC(4/5)
235 GGATC
BalI 147 TGGCCA TGG^CCA
441 TGGCCA
BfaI 209 CTAG C^TAG
296 CTAG
BpmI 944 CTCCAG CTGGAG(16/14)
848 CTGGAG
BsmFI 474 GGGAC GGGAC(10/14)
806 GTCCC
BstBI 350 TTCGAA TT^CGAA
507 TTCGAA
BstF5I 480 GGATG GGATG(2/0)
710 GGATG
Cac8I 765 GCCAGC GC[ACGT]^[ACGT]GC
950 GCAAGC
DdeI 182 CTCAG C^T[ACGT]AG
890 CTAAG
EaeI 147 TGGCCA [CT]^GGCC[GA]
441 TGGCCA
FokI 480 GGATG GGATG(9/13)
710 GGATG
MaeI 209 CTAG C^TAG
296 CTAG
MaeIII 725 GTGAC ^GT[ACGT]AC
878 GTGAC
MluNI 147 TGGCCA TGG^CCA
441 TGGCCA
MscI 147 TGGCCA TGG^CCA
441 TGGCCA
NlaIII 549 CATG CATG^
757 CATG
NspV 350 TTCGAA TT^CGAA
507 TTCGAA
PstI 65 CTGCAG CTGCA^G
917 CTGCAG
SfcI 65 CTGCAG C^T[GA][CT]AG
917 CTGCAG
SfuI 350 TTCGAA TT^CGAA
507 TTCGAA
Tsp45I 725 GTGAC ^GT[GC]AC
878 GTGAC
TspRI 312 CAGTG CA[GC]TG[ACGT][ACGT]^
535 CAGTG
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# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AcsI AluI ApaI ApoI AvaI
AvaII BanII BbvI BcnI BmyI
BsaJI BsoBI Bsp1286I BspMI BsrI
BssKI BstNI Cfr13I DpnII DraII
EcoO109I EcoRII Fnu4HI HaeIII HapII
HinfI HpaII HphI ItaI MboI
MboII MnlI MspI MvaI MwoI
NciI NdeII NlaIV PpuMI Sau3AI
Sau96I ScrFI SfaNI SmaI TaqI
TfiI TseI Tsp509I TthHB8I XmaCI
XmaI