Restriction map for
pFN21AE6679
(Linear) (Six-base) MAPSORT of: KIEE6679.seq Check: 4555 from: 1 to: 984
KIEE6679.orf
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 240 910 984
Size: 240 670 74
Fragments arranged by size:
670 240 74
AccI GT'mk_AC
Cuts at: 0 278 984
Size: 278 706
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 267 344 984
Size: 267 77 640
Fragments arranged by size:
640 267 77
ApaI G_GGCC'C
Cuts at: 0 844 898 907 984
Size: 844 54 9 77
Fragments arranged by size:
844 77 54 9
ApoI r'AATT_y
Cuts at: 0 17 300 510 984
Size: 17 283 210 474
Fragments arranged by size:
474 283 210 17
AseI AT'TA_AT
Cuts at: 0 437 984
Size: 437 547
AvaI C'yCGr_G
Cuts at: 0 810 829 900 909 984
Size: 810 19 71 9 75
Fragments arranged by size:
810 75 71 19 9
BanII G_rGCy'C
Cuts at: 0 844 898 907 945 984
Size: 844 54 9 38 39
Fragments arranged by size:
844 54 39 38 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 10 984
Size: 10 974
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 488 522 984
Size: 488 34 462
Fragments arranged by size:
488 462 34
BglI GCCn_nnn'nGGC
Cuts at: 0 902 984
Size: 902 82
Bme1580I G_kGCm'C
Cuts at: 0 844 898 907 984
Size: 844 54 9 77
Fragments arranged by size:
844 77 54 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 305 528 984
Size: 305 223 456
Fragments arranged by size:
456 305 223
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 869 929 984
Size: 869 60 55
Fragments arranged by size:
869 60 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 984
Size: 665 319
BsaBI GATnn'nnATC
Cuts at: 0 715 984
Size: 715 269
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 579 984
Size: 579 405
BseYI C'CCAG_C
Cuts at: 0 657 781 984
Size: 657 124 203
Fragments arranged by size:
657 203 124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 984
Size: 60 924
BsiHKAI G_wGCw'C
Cuts at: 0 945 984
Size: 945 39
Bsp1286I G_dGCh'C
Cuts at: 0 844 898 907 945 984
Size: 844 54 9 38 39
Fragments arranged by size:
844 54 39 38 9
BspHI T'CATG_A
Cuts at: 0 548 984
Size: 548 436
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 16 240 910 984
Size: 16 224 670 74
Fragments arranged by size:
670 224 74 16
BsrDI GCAATG_nn'
Cuts at: 0 244 984
Size: 244 740
BstBI TT'CG_AA
Cuts at: 0 351 508 984
Size: 351 157 476
Fragments arranged by size:
476 351 157
BstEII G'GTnAC_C
Cuts at: 0 877 984
Size: 877 107
Bsu36I CC'TnA_GG
Cuts at: 0 890 984
Size: 890 94
ClaI AT'CG_AT
Cuts at: 0 863 984
Size: 863 121
EaeI y'GGCC_r
Cuts at: 0 147 441 984
Size: 147 294 543
Fragments arranged by size:
543 294 147
EarI CTCTTCn'nnn_
Cuts at: 0 207 984
Size: 207 777
EcoICRI GAG'CTC
Cuts at: 0 943 984
Size: 943 41
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 267 344 869 929 984
Size: 267 77 525 60 55
Fragments arranged by size:
525 267 77 60 55
EcoO109I rG'GnC_Cy
Cuts at: 0 805 823 841 886 894 895 904 913
Size: 805 18 18 45 8 1 9 9
Cuts at: 913 984
Size: 71
Fragments arranged by size:
805 71 45 18 18 9 9 8
1
HaeII r_GCGC'y
Cuts at: 0 971 984
Size: 971 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 80 112 163 195 984
Size: 80 32 51 32 789
Fragments arranged by size:
789 80 51 32 32
HincII GTy'rAC
Cuts at: 0 588 984
Size: 588 396
MfeI C'AATT_G
Cuts at: 0 632 984
Size: 632 352
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 984
Size: 316 668
MscI TGG'CCA
Cuts at: 0 149 443 984
Size: 149 294 541
Fragments arranged by size:
541 294 149
MspA1I CmG'CkG
Cuts at: 0 70 984
Size: 70 914
PasI CC'CwG_GG
Cuts at: 0 56 984
Size: 56 928
PfoI T'CCnGG_A
Cuts at: 0 47 984
Size: 47 937
PpuMI rG'GwC_Cy
Cuts at: 0 805 823 886 913 984
Size: 805 18 63 27 71
Fragments arranged by size:
805 71 63 27 18
PshAI GACnn'nnGTC
Cuts at: 0 884 984
Size: 884 100
PspOMI G'GGCC_C
Cuts at: 0 840 894 903 984
Size: 840 54 9 81
Fragments arranged by size:
840 81 54 9
PstI C_TGCA'G
Cuts at: 0 69 921 984
Size: 69 852 63
Fragments arranged by size:
852 69 63
PvuII CAG'CTG
Cuts at: 0 70 984
Size: 70 914
SacI G_AGCT'C
Cuts at: 0 945 984
Size: 945 39
SapI GCTCTTCn'nnn_
Cuts at: 0 207 984
Size: 207 777
SbfI CC_TGCA'GG
Cuts at: 0 921 984
Size: 921 63
SfcI C'TryA_G
Cuts at: 0 65 917 984
Size: 65 852 67
Fragments arranged by size:
852 67 65
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 902 984
Size: 902 82
SmaI CCC'GGG
Cuts at: 0 812 831 902 911 984
Size: 812 19 71 9 73
Fragments arranged by size:
812 73 71 19 9
SmlI C'TyrA_G
Cuts at: 0 680 984
Size: 680 304
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 896 984
Size: 896 88
XbaI T'CTAG_A
Cuts at: 0 208 984
Size: 208 776
XmaI C'CCGG_G
Cuts at: 0 810 829 900 909 984
Size: 810 19 71 9 75
Fragments arranged by size:
810 75 71 19 9
Enzymes that do cut:
AarI AccI AcuI ApaI ApoI AseI AvaI BanII
BbsI BcgI BglI Bme1580I BmrI BpmI BpuEI BsaBI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI
BstBI BstEII Bsu36I ClaI EaeI EarI EcoICRI Eco57MI
EcoO109I HaeII Hin4I HincII MfeI MmeI MscI MspA1I
PasI PfoI PpuMI PshAI PspOMI PstI PvuII SacI
SapI SbfI SfcI SfiI SmaI SmlI TaqII XbaI
XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI AscI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BciVI BclI BfrBI
BglII BlpI BmgBI BmtI BplI Bpu10I BsaI BsaAI
BsaHI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstXI BstYI
BstZ17I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KasI KpnI MluI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PflMI PmeI PmlI PpiI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI ScaI
SexAI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TatI TstI Tth111I XcmI
XhoI XmnI ZraI