################################## # NO CUT ENZYMES ( 209 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeII MaeIII MamI MfeI MflI MluI MroI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI TseI Tsp45I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AciI 345 GCGG CCGC(-3/-1) AspHI 53 GAGCTC G[AT]GC[AT]^C AvaII 187 GGACC G^G[AT]CC BalI 294 TGGCCA TGG^CCA BfaI 508 CTAG C^TAG BpmI 259 CTGGAG CTGGAG(16/14) BsiHKAI 53 GAGCTC G[AT]GC[AT]^C BsmAI 154 GAGAC GTCTC(1/5) BssKI 68 CCTGG ^CC[ACGT]GG BstF5I 65 CATCC GGATG(2/0) BstNI 68 CCTGG CC^[AT]GG Cfr13I 187 GGACC G^G[ACGT]CC DpnII 62 GATC ^GATC EaeI 294 TGGCCA [CT]^GGCC[GA] EcoRII 68 CCTGG ^CC[AT]GG Fnu4HI 345 GCGGC GC^[ACGT]GC FokI 65 CATCC GGATG(9/13) HaeIII 295 GGCC GG^CC ItaI 345 GCGGC GC^[ACGT]GC MaeI 508 CTAG C^TAG MboI 62 GATC ^GATC MboII 217 TCTTC GAAGA(8/7) MluNI 294 TGGCCA TGG^CCA MscI 294 TGGCCA TGG^CCA MvaI 68 CCTGG CC^[AT]GG MwoI 304 GCTCCCAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeII 62 GATC ^GATC NspI 426 GCATGT [GA]CATG^[CT] SacI 53 GAGCTC GAGCT^C Sau3AI 62 GATC ^GATC Sau96I 187 GGACC G^G[ACGT]CC ScrFI 68 CCTGG CC^[ACGT]GG SmlI 47 CTCAAG C^T[CT][GA]AG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### BanII 53 GAGCTC G[GA]GC[CT]^C 166 GAGCCC BmyI 53 GAGCTC G[AGT]GC[ACT]^C 166 GAGCCC BsiYI 119 CCTTCAGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 197 CCTCAAAATGG BslI 119 CCTTCAGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 197 CCTCAAAATGG BsmFI 412 GGGAC GGGAC(10/14) 434 GGGAC Bsp1286I 53 GAGCTC G[AGT]GC[ACT]^C 166 GAGCCC BsrI 251 ACTGG ACTGG(1/-1) 491 ACTGG MseI 31 TTAA T^TAA 286 TTAA NlaIII 366 CATG CATG^ 427 CATG NlaIV 165 GGAGCC GG[ACGT]^[ACGT]CC 303 GGCTCC SfaNI 214 GCATC GCATC(5/9) 465 GCATC Tru9I 31 TTAA T^TAA 286 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 4 enzymes) # ######################################### AluI DdeI MnlI Tsp509I