################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII AccII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseRI BsiEI BsiWI BsmBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI KasI KspI MaeII MaeIII MamI MluI MseI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp45I Tth111I TthHB8I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 1129 GGTACC G^GTACC AccIII 5 TCCGGA T^CCGGA AciI 30 GCGG CCGC(-3/-1) AlwI 607 GGATC GGATC(4/5) AlwNI 338 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 471 GGGCCC GGGCC^C Asp718I 1129 GGTACC G^GTACC AvaI 410 CCCGGG C^[CT]CG[GA]G BglI 790 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaWI 5 TCCGGA [AT]^CCGG[AT] BseAI 5 TCCGGA T^CCGGA BsmAI 847 GTCTC GTCTC(1/5) BsmI 638 GAATGC GAATGC(1/-1) BsoBI 410 CCCGGG C^[CT]CG[GA]G BspEI 5 TCCGGA T^CCGGA BspMI 628 GCAGGT ACCTGC(4/8) BstAPI 60 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1079 CCAACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 607 GGATCT [GA]^GATC[CT] DpnII 608 GATC ^GATC EcoRV 494 GATATC GAT^ATC HgaI 52 GCGTC GACGC(5/10) HinfI 870 GACTC G^A[ACGT]TC HphI 479 TCACC GGTGA(8/7) KpnI 1129 GGTACC GGTAC^C MboI 608 GATC ^GATC MfeI 128 CAATTG C^AATTG MflI 607 GGATCT [GA]^GATC[CT] MroI 5 TCCGGA T^CCGGA MunI 128 CAATTG C^AATTG NcoI 683 CCATGG C^CATGG NdeII 608 GATC ^GATC PleI 870 GACTC GAGTC(4/5) PpuMI 562 GGGACCT [GA]G^G[AT]CC[CT] PstI 914 CTGCAG CTGCA^G SacI 728 GAGCTC GAGCT^C Sau3AI 608 GATC ^GATC SmaI 410 CCCGGG CCC^GGG Tsp509I 129 AATT ^AATT XhoII 607 GGATCT [GA]^GATC[CT] XmaCI 410 CCCGGG C^CCGGG XmaI 410 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AccI 652 GTCTAC GT^[AC][GT]AC 1145 GTCTAC BfaI 290 CTAG C^TAG 1018 CTAG BsgI 181 CTGCAC GTGCAG(16/14) 770 CTGCAC DdeI 354 CTCAG C^T[ACGT]AG 854 CTGAG DsaI 266 CCGTGG C^C[GA][CT]GG 683 CCATGG EarI 174 CTCTTC CTCTTC(1/4) 400 GAAGAG HincII 169 GTCAAC GT[CT]^[GA]AC 457 GTCAAC HindII 169 GTCAAC GT[CT]^[GA]AC 457 GTCAAC Ksp632I 174 CTCTTC CTCTTC(1/4) 400 GAAGAG MaeI 290 CTAG C^TAG 1018 CTAG NspI 104 ACATGC [GA]CATG^[CT] 210 ACATGC PvuII 61 CAGCTG CAG^CTG 1009 CAGCTG SfcI 832 CTACAG C^T[GA][CT]AG 914 CTGCAG XcmI 935 CCAACATGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1001 CCAAGTGGCAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AfaI AluI AspHI AvaII BalI BanI BanII BbvI BcnI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DraII EaeI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HpaII ItaI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV RsaI Sau96I ScrFI SfaNI StyI TseI TspRI