(Circular) (Six-base) MAPSORT of: pFN21AE4237.seq Check: 4250 from: 1 to: 7846 pFN21AE4237 7846 bp With 182 enzymes: SgfI * December 8, 2014 13:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5656 278 Size: 53 83 186 936 4120 2468 Fragments arranged by size: 4120 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5123 5189 1937 Size: 3186 66 4594 Fragments arranged by size: 4594 3186 66 Acc65I G'GTAC_C Cuts at: 5167 5167 Size: 7846 AclI AA'CG_TT Cuts at: 1563 3469 5973 6346 1563 Size: 1906 2504 373 3063 Fragments arranged by size: 3063 2504 1906 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4575 5909 7293 7561 1632 Size: 2943 1334 1384 268 1917 Fragments arranged by size: 2943 1917 1384 1334 268 AflII C'TTAA_G Cuts at: 820 1017 3314 820 Size: 197 2297 5352 Fragments arranged by size: 5352 2297 197 AflIII A'CryG_T Cuts at: 1203 4231 6745 7722 1203 Size: 3028 2514 977 1327 Fragments arranged by size: 3028 2514 1327 977 AgeI A'CCGG_T Cuts at: 6677 6677 Size: 7846 AhdI GACnn_n'nnGTC Cuts at: 3108 6575 3108 Size: 3467 4379 AleI CACnn'nnGTG Cuts at: 1166 3648 1166 Size: 2482 5364 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7846 AlwNI CAG_nnn'CTG Cuts at: 2891 5589 7161 2891 Size: 2698 1572 3576 Fragments arranged by size: 3576 2698 1572 ApaLI G'TGCA_C Cuts at: 5055 5903 7059 5055 Size: 848 1156 5842 Fragments arranged by size: 5842 1156 848 ApoI r'AATT_y Cuts at: 1493 2968 3478 4178 4838 5155 5381 5533 7642 Size: 1475 510 700 660 317 226 152 2109 Cuts at: 7642 1493 Size: 1697 Fragments arranged by size: 2109 1697 1475 700 660 510 317 226 152 AseI AT'TA_AT Cuts at: 160 1032 3560 6400 160 Size: 872 2528 2840 1606 Fragments arranged by size: 2840 2528 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7846 AvaI C'yCGr_G Cuts at: 1870 1943 2387 2453 5171 1870 Size: 73 444 66 2718 4545 Fragments arranged by size: 4545 2718 444 73 66 AvrII C'CTAG_G Cuts at: 2218 2218 Size: 7846 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2865 2898 2865 Size: 33 7813 BamHI G'GATC_C Cuts at: 5176 5543 5176 Size: 367 7479 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5167 6622 618 Size: 325 217 627 3380 1455 1842 Fragments arranged by size: 3380 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2084 4857 5165 1391 Size: 693 2773 308 4072 Fragments arranged by size: 4072 2773 693 308 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2740 3009 928 Size: 971 841 269 5765 Fragments arranged by size: 5765 971 841 269 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6037 6071 1903 Size: 34 4100 34 3678 Fragments arranged by size: 4100 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5738 6948 5738 Size: 1210 6636 BclI T'GATC_A Cuts at: 1548 4905 1548 Size: 3357 4489 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 6457 136 Size: 107 122 71 631 605 337 4448 1525 Fragments arranged by size: 4448 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 3565 7841 3565 Size: 4276 3570 BlpI GC'TnA_GC Cuts at: 4079 5263 4079 Size: 1184 6662 Bme1580I G_kGCm'C Cuts at: 1790 2245 5059 5907 7063 1790 Size: 455 2814 848 1156 2573 Fragments arranged by size: 2814 2573 1156 848 455 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7846 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2255 2810 3818 Size: 416 53 83 399 24 838 555 1008 Cuts at: 3818 3905 5577 6535 442 Size: 87 1672 958 1753 Fragments arranged by size: 1753 1672 1008 958 838 555 416 399 87 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7846 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2068 2079 3672 6506 1239 Size: 829 11 1593 2834 2579 Fragments arranged by size: 2834 2579 1593 829 11 Bpu10I CC'TnA_GC Cuts at: 4848 4848 Size: 7846 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5971 6836 7134 5971 Size: 865 298 6683 Fragments arranged by size: 6683 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2159 6509 882 Size: 617 660 4350 2219 Fragments arranged by size: 4350 2219 660 617 BsaAI yAC'GTr Cuts at: 493 1204 5068 5637 493 Size: 711 3864 569 2702 Fragments arranged by size: 3864 2702 711 569 BsaBI GATnn'nnATC Cuts at: 3279 7840 3279 Size: 4561 3285 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5653 6035 6670 275 Size: 53 83 186 936 4120 382 635 1451 Fragments arranged by size: 4120 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2168 3174 4945 6279 6677 6951 7098 7509 2168 Size: 1006 1771 1334 398 274 147 411 2505 Fragments arranged by size: 2505 1771 1334 1006 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2515 2545 1178 Size: 30 165 30 1112 30 6479 Fragments arranged by size: 6479 1112 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2045 2076 2513 2520 1186 Size: 859 31 437 7 6512 Fragments arranged by size: 6512 859 437 31 7 BseYI C'CCAG_C Cuts at: 7049 7545 7049 Size: 496 7350 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4835 1163 Size: 573 3099 4174 Fragments arranged by size: 4174 3099 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2016 5296 6057 7085 1540 Size: 275 186 15 3280 761 1028 2301 Fragments arranged by size: 3280 2301 1028 761 275 186 15 BsiHKAI G_wGCw'C Cuts at: 4135 5059 5907 5992 7063 4135 Size: 924 848 85 1071 4918 Fragments arranged by size: 4918 1071 924 848 85 BsmI GAATG_Cn' Cuts at: 4960 5357 5450 4960 Size: 397 93 7356 Fragments arranged by size: 7356 397 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2084 2245 4135 4857 5059 5165 5907 Size: 399 294 161 1890 722 202 106 742 Cuts at: 5907 5992 7063 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1890 1071 742 722 399 294 202 161 106 85 BspEI T'CCGG_A Cuts at: 3174 4945 7509 3174 Size: 1771 2564 3511 Fragments arranged by size: 3511 2564 1771 BspHI T'CATG_A Cuts at: 3595 5735 7465 3595 Size: 2140 1730 3976 Fragments arranged by size: 3976 2140 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2439 5201 844 Size: 1595 2762 3489 Fragments arranged by size: 3489 2762 1595 BsrBI CCG'CTC Cuts at: 1394 1823 2528 5733 1394 Size: 429 705 3205 3507 Fragments arranged by size: 3507 3205 705 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4994 6341 6515 65 Size: 1183 3746 1347 174 1396 Fragments arranged by size: 3746 1396 1347 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2021 2312 3243 6490 6677 1700 Size: 110 211 291 931 3247 187 2869 Fragments arranged by size: 3247 2869 931 291 211 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7846 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7846 BssSI C'ACGA_G Cuts at: 5906 6918 5906 Size: 1012 6834 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7846 BstBI TT'CG_AA Cuts at: 5032 6650 5032 Size: 1618 6228 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7846 BstYI r'GATC_y Cuts at: 1972 3034 3565 4003 5176 5543 5928 5945 7397 Size: 1062 531 438 1173 367 385 17 1452 Cuts at: 7397 7483 7495 7841 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1173 1062 531 438 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 5124 5124 Size: 7846 Bsu36I CC'TnA_GG Cuts at: 2588 2588 Size: 7846 BtgI C'CryG_G Cuts at: 513 1065 2121 3061 4739 513 Size: 552 1056 940 1678 3620 Fragments arranged by size: 3620 1678 1056 940 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7107 Fragments arranged by size: 7107 683 56 BtsI GCAGTG_nn' Cuts at: 2032 2106 4416 5362 6156 6176 2032 Size: 74 2310 946 794 20 3702 Fragments arranged by size: 3702 2310 946 794 74 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7846 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4363 4398 4593 4628 4815 4850 529 Size: 35 3799 35 195 35 187 35 3525 Fragments arranged by size: 3799 3525 195 187 35 35 35 35 DraI TTT'AAA Cuts at: 3202 4183 4461 5150 3202 Size: 981 278 689 5898 Fragments arranged by size: 5898 981 689 278 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4719 6853 809 Size: 507 3403 2134 1802 Fragments arranged by size: 3403 2134 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 2310 2511 Size: 54 1425 50 275 18 183 297 201 Cuts at: 2511 4260 5293 6182 7811 8 Size: 1749 1033 889 1629 43 Fragments arranged by size: 1749 1629 1425 1033 889 297 275 201 183 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2013 5293 1537 Size: 275 201 3280 4090 Fragments arranged by size: 4090 3280 275 201 EarI CTCTTCn'nnn_ Cuts at: 1353 2499 3009 3918 4161 5776 1353 Size: 1146 510 909 243 1615 3423 Fragments arranged by size: 3423 1615 1146 909 510 243 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2208 6433 6807 6953 387 Size: 1064 138 619 4225 374 146 1280 Fragments arranged by size: 4225 1280 1064 619 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2068 2079 3672 4575 5909 6506 7293 Size: 393 436 11 1593 903 1334 597 787 Cuts at: 7293 7561 1239 Size: 268 1524 Fragments arranged by size: 1593 1524 1334 903 787 597 436 393 268 11 EcoNI CCTnn'n_nnAGG Cuts at: 2591 3776 2591 Size: 1185 6661 EcoO109I rG'GnC_Cy Cuts at: 2646 3135 4116 2646 Size: 489 981 6376 Fragments arranged by size: 6376 981 489 EcoRI G'AATT_C Cuts at: 5155 5533 5155 Size: 378 7468 EcoRV GAT'ATC Cuts at: 4012 4012 Size: 7846 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3430 3461 3430 Size: 31 7815 FspI TGC'GCA Cuts at: 6352 6352 Size: 7846 HaeII r_GCGC'y Cuts at: 1678 6993 1678 Size: 5315 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2515 2547 3094 3126 3700 Size: 32 135 32 943 32 547 32 574 Cuts at: 3700 3732 4754 4786 6487 6519 6561 6593 1373 Size: 32 1022 32 1701 32 42 32 2626 Fragments arranged by size: 2626 1701 1022 943 574 547 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2523 2686 5190 5436 669 Size: 988 281 585 163 2504 246 3079 Fragments arranged by size: 3079 2504 988 585 281 246 163 HindIII A'AGCT_T Cuts at: 748 3084 4295 5078 748 Size: 2336 1211 783 3516 Fragments arranged by size: 3516 2336 1211 783 HpaI GTT'AAC Cuts at: 5436 5436 Size: 7846 KpnI G_GTAC'C Cuts at: 5171 5171 Size: 7846 MfeI C'AATT_G Cuts at: 5445 5445 Size: 7846 MluI A'CGCG_T Cuts at: 7722 7722 Size: 7846 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2558 3236 3452 6960 7144 1305 Size: 1253 678 216 3508 184 2007 Fragments arranged by size: 3508 2007 1253 678 216 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2513 4262 10 Size: 54 1425 343 681 1749 3594 Fragments arranged by size: 3594 1749 1425 681 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3648 4234 4236 4394 5022 5804 6163 Size: 648 2482 586 2 158 628 782 359 Cuts at: 6163 6322 518 Size: 159 2042 Fragments arranged by size: 2482 2042 782 648 628 586 359 159 158 2 MspA1I CmG'CkG Cuts at: 1606 2078 2123 5262 5939 7087 7332 1606 Size: 472 45 3139 677 1148 245 2120 Fragments arranged by size: 3139 2120 1148 677 472 245 45 NaeI GCC'GGC Cuts at: 2023 2023 Size: 7846 NcoI C'CATG_G Cuts at: 513 1065 3061 513 Size: 552 1996 5298 Fragments arranged by size: 5298 1996 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7846 NgoMIV G'CCGG_C Cuts at: 2021 2021 Size: 7846 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7846 NotI GC'GGCC_GC Cuts at: 5293 5293 Size: 7846 NspI r_CATG'y Cuts at: 1132 1229 4058 4235 5204 6749 1132 Size: 97 2829 177 969 1545 2229 Fragments arranged by size: 2829 2229 1545 969 177 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7846 PciI A'CATG_T Cuts at: 4231 6745 4231 Size: 2514 5332 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7846 PmeI GTTT'AAAC Cuts at: 5150 5150 Size: 7846 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3336 3368 5915 5947 7428 7460 3336 Size: 32 2547 32 1481 32 3722 Fragments arranged by size: 3722 2547 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2646 4116 2646 Size: 1470 6376 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7846 PsiI TTA'TAA Cuts at: 2621 3090 3252 3420 5416 2621 Size: 469 162 168 1996 5051 Fragments arranged by size: 5051 1996 469 168 162 PstI C_TGCA'G Cuts at: 830 3122 3723 5198 830 Size: 2292 601 1475 3478 Fragments arranged by size: 3478 2292 1475 601 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7846 SacII CC_GC'GG Cuts at: 2124 2124 Size: 7846 SalI G'TCGA_C Cuts at: 1936 5188 1936 Size: 3252 4594 SanDI GG'GwC_CC Cuts at: 4116 4116 Size: 7846 SbfI CC_TGCA'GG Cuts at: 5198 5198 Size: 7846 ScaI AGT'ACT Cuts at: 3351 6094 7806 3351 Size: 2743 1712 3391 Fragments arranged by size: 3391 2743 1712 SfcI C'TryA_G Cuts at: 826 1045 3118 3719 4953 5194 6329 7010 7201 Size: 219 2073 601 1234 241 1135 681 191 Cuts at: 7201 826 Size: 1471 Fragments arranged by size: 2073 1471 1234 1135 681 601 241 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7846 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7846 SmaI CCC'GGG Cuts at: 1872 5173 1872 Size: 3301 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3314 5950 6851 7113 820 Size: 197 926 1371 2636 901 262 1553 Fragments arranged by size: 2636 1553 1371 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5637 493 Size: 5144 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7846 SphI G_CATG'C Cuts at: 1132 4058 5204 1132 Size: 2926 1146 3774 Fragments arranged by size: 3774 2926 1146 SspI AAT'ATT Cuts at: 5 52 3190 4581 5770 5 Size: 47 3138 1391 1189 2081 Fragments arranged by size: 3138 2081 1391 1189 47 StuI AGG'CCT Cuts at: 1517 2991 1517 Size: 1474 6372 StyI C'CwwG_G Cuts at: 513 1065 2218 3061 5285 513 Size: 552 1153 843 2224 3074 Fragments arranged by size: 3074 2224 1153 843 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2177 4263 4617 5873 5890 6043 6228 Size: 1255 597 2086 354 1256 17 153 185 Cuts at: 6228 6673 325 Size: 445 1498 Fragments arranged by size: 2086 1498 1256 1255 597 445 354 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2653 3349 4170 Size: 274 80 33 51 1443 676 696 821 Cuts at: 4170 6092 7804 96 Size: 1922 1712 138 Fragments arranged by size: 1922 1712 1443 821 696 676 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3156 3188 7806 7838 3156 Size: 32 4618 32 3164 Fragments arranged by size: 4618 3164 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7846 XbaI T'CTAG_A Cuts at: 4964 5182 4964 Size: 218 7628 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2541 2541 Size: 7846 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7846 XmaI C'CCGG_G Cuts at: 1870 5171 1870 Size: 3301 4545 XmnI GAAnn'nnTTC Cuts at: 3189 4071 4580 4599 5100 5975 3189 Size: 882 509 19 501 875 5060 Fragments arranged by size: 5060 882 875 509 501 19 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5654 276 Size: 53 83 186 936 4120 2468 Fragments arranged by size: 4120 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI DraIII EcoICRI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI