Restriction map for pFN21AE4237
 (Linear) (Six-base) MAPSORT of: KIEE4237.seq  Check: 8856  from: 1  to: 3141



KIEE4237.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 12:57  ..

AccI GT'mk_AC

Cuts at:      0    3118    3141 
   Size:       3118      23
 
AclI AA'CG_TT

Cuts at:      0    1464    3141 
   Size:       1464    1677
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2570    3141 
   Size:       2570     571
 
AflII C'TTAA_G

Cuts at:      0    1309    3141 
   Size:       1309    1832
 
AflIII A'CryG_T

Cuts at:      0    2226    3141 
   Size:       2226     915
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1103    3141 
   Size:       1103    2038
 
AleI CACnn'nnGTG

Cuts at:      0    1643    3141 
   Size:       1643    1498
 
AlwNI CAG_nnn'CTG

Cuts at:      0     886    3141 
   Size:        886    2255
 
ApaLI G'TGCA_C

Cuts at:      0    3050    3141 
   Size:       3050      91
 
ApoI r'AATT_y

Cuts at:      0     963    1473    2173    2833    3141 
   Size:        963     510     700     660     308

  Fragments arranged by size: 

                 963     700     660     510     308
 
AseI AT'TA_AT

Cuts at:      0    1555    3141 
   Size:       1555    1586
 
AvaI C'yCGr_G

Cuts at:      0     382     448    3141 
   Size:        382      66    2693

  Fragments arranged by size: 

                2693     382      66
 
AvrII C'CTAG_G

Cuts at:      0     213    3141 
   Size:        213    2928
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     860     893    3141 
   Size:        860      33    2248

  Fragments arranged by size: 

                2248     860      33
 
BanII G_rGCy'C

Cuts at:      0      79    2852    3141 
   Size:         79    2773     289

  Fragments arranged by size: 

                2773     289      79
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     735    1004    3141 
   Size:        735     269    2137

  Fragments arranged by size: 

                2137     735     269
 
BclI T'GATC_A

Cuts at:      0    2900    3141 
   Size:       2900     241
 
BglII A'GATC_T

Cuts at:      0    1560    3141 
   Size:       1560    1581
 
BlpI GC'TnA_GC

Cuts at:      0    2074    3141 
   Size:       2074    1067
 
Bme1580I G_kGCm'C

Cuts at:      0     240    3054    3141 
   Size:        240    2814      87

  Fragments arranged by size: 

                2814     240      87
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     250     805    1813    1900    3141 
   Size:        250     555    1008      87    1241

  Fragments arranged by size: 

                1241    1008     555     250      87
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      63      74    1667    3141 
   Size:         63      11    1593    1474

  Fragments arranged by size: 

                1593    1474      63      11
 
Bpu10I CC'TnA_GC

Cuts at:      0    2843    3141 
   Size:       2843     298
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     154    3141 
   Size:        154    2987
 
BsaAI yAC'GTr

Cuts at:      0    3063    3141 
   Size:       3063      78
 
BsaBI GATnn'nnATC

Cuts at:      0    1274    3141 
   Size:       1274    1867
 
BsaWI w'CCGG_w

Cuts at:      0     163    1169    2940    3141 
   Size:        163    1006    1771     201

  Fragments arranged by size: 

                1771    1006     201     163
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     510     540    3141 
   Size:        510      30    2601

  Fragments arranged by size: 

                2601     510      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      40      71     508     515    3141 
   Size:         40      31     437       7    2626

  Fragments arranged by size: 

                2626     437      40      31       7
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2830    3141 
   Size:       2830     311
 
BsiEI CG_ry'CG

Cuts at:      0      11    3141 
   Size:         11    3130
 
BsiHKAI G_wGCw'C

Cuts at:      0    2130    3054    3141 
   Size:       2130     924      87

  Fragments arranged by size: 

                2130     924      87
 
BsmI GAATG_Cn'

Cuts at:      0    2955    3141 
   Size:       2955     186
 
Bsp1286I G_dGCh'C

Cuts at:      0      79     240    2130    2852    3054    3141 
   Size:         79     161    1890     722     202      87

  Fragments arranged by size: 

                1890     722     202     161      87      79
 
BspEI T'CCGG_A

Cuts at:      0    1169    2940    3141 
   Size:       1169    1771     201

  Fragments arranged by size: 

                1771    1169     201
 
BspHI T'CATG_A

Cuts at:      0    1590    3141 
   Size:       1590    1551
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     434    3141 
   Size:        434    2707
 
BsrBI CCG'CTC

Cuts at:      0     523    3141 
   Size:        523    2618
 
BsrDI GCAATG_nn'

Cuts at:      0    2989    3141 
   Size:       2989     152
 
BsrFI r'CCGG_y

Cuts at:      0      16     307    1238    3141 
   Size:         16     291     931    1903

  Fragments arranged by size: 

                1903     931     291      16
 
BstBI TT'CG_AA

Cuts at:      0    3027    3141 
   Size:       3027     114
 
BstYI r'GATC_y

Cuts at:      0    1029    1560    1998    3141 
   Size:       1029     531     438    1143

  Fragments arranged by size: 

                1143    1029     531     438
 
BstZ17I GTA'TAC

Cuts at:      0    3119    3141 
   Size:       3119      22
 
Bsu36I CC'TnA_GG

Cuts at:      0     583    3141 
   Size:        583    2558
 
BtgI C'CryG_G

Cuts at:      0     116    1056    2734    3141 
   Size:        116     940    1678     407

  Fragments arranged by size: 

                1678     940     407     116
 
BtsI GCAGTG_nn'

Cuts at:      0      27     101    2411    3141 
   Size:         27      74    2310     730

  Fragments arranged by size: 

                2310     730      74      27
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2358    2393    2588    2623    2810    2845    3141 
   Size:       2358      35     195      35     187      35     296

  Fragments arranged by size: 

                2358     296     195     187      35      35      35
 
DraI TTT'AAA

Cuts at:      0    1197    2178    2456    3141 
   Size:       1197     981     278     685

  Fragments arranged by size: 

                1197     981     685     278
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2714    3141 
   Size:       2714     427
 
EaeI y'GGCC_r

Cuts at:      0       8     305     506    2255    3141 
   Size:          8     297     201    1749     886

  Fragments arranged by size: 

                1749     886     297     201       8
 
EagI C'GGCC_G

Cuts at:      0       8    3141 
   Size:          8    3133
 
EarI CTCTTCn'nnn_

Cuts at:      0     494    1004    1913    2156    3141 
   Size:        494     510     909     243     985

  Fragments arranged by size: 

                 985     909     510     494     243
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     203    3141 
   Size:        203    2938
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      63      74    1667    2570    3141 
   Size:         63      11    1593     903     571

  Fragments arranged by size: 

                1593     903     571      63      11
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     586    1771    3141 
   Size:        586    1185    1370

  Fragments arranged by size: 

                1370    1185     586
 
EcoO109I rG'GnC_Cy

Cuts at:      0     641    1130    2111    3141 
   Size:        641     489     981    1030

  Fragments arranged by size: 

                1030     981     641     489
 
EcoRV GAT'ATC

Cuts at:      0    2007    3141 
   Size:       2007    1134
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1425    1456    3141 
   Size:       1425      31    1685

  Fragments arranged by size: 

                1685    1425      31
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     510     542    1089    1121    1695    1727    2749    2781 
   Size:        510      32     547      32     574      32    1022      32

Cuts at:   2781    3141 
   Size:        360

  Fragments arranged by size: 

                1022     574     547     510     360      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     518     681    3141 
   Size:        518     163    2460

  Fragments arranged by size: 

                2460     518     163
 
HindIII A'AGCT_T

Cuts at:      0    1079    2290    3073    3141 
   Size:       1079    1211     783      68

  Fragments arranged by size: 

                1211    1079     783      68
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     553    1231    1447    3141 
   Size:        553     678     216    1694

  Fragments arranged by size: 

                1694     678     553     216
 
MscI TGG'CCA

Cuts at:      0     508    2257    3141 
   Size:        508    1749     884

  Fragments arranged by size: 

                1749     884     508
 
MslI CAynn'nnrTG

Cuts at:      0    1643    2229    2231    2389    3017    3141 
   Size:       1643     586       2     158     628     124

  Fragments arranged by size: 

                1643     628     586     158     124       2
 
MspA1I CmG'CkG

Cuts at:      0      73     118    3141 
   Size:         73      45    3023

  Fragments arranged by size: 

                3023      73      45
 
NaeI GCC'GGC

Cuts at:      0      18    3141 
   Size:         18    3123
 
NcoI C'CATG_G

Cuts at:      0    1056    3141 
   Size:       1056    2085
 
NgoMIV G'CCGG_C

Cuts at:      0      16    3141 
   Size:         16    3125
 
NspI r_CATG'y

Cuts at:      0    2053    2230    3141 
   Size:       2053     177     911

  Fragments arranged by size: 

                2053     911     177
 
PciI A'CATG_T

Cuts at:      0    2226    3141 
   Size:       2226     915
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1331    1363    3141 
   Size:       1331      32    1778

  Fragments arranged by size: 

                1778    1331      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     641    2111    3141 
   Size:        641    1470    1030

  Fragments arranged by size: 

                1470    1030     641
 
PsiI TTA'TAA

Cuts at:      0     616    1085    1247    1415    3141 
   Size:        616     469     162     168    1726

  Fragments arranged by size: 

                1726     616     469     168     162
 
PstI C_TGCA'G

Cuts at:      0    1117    1718    3141 
   Size:       1117     601    1423

  Fragments arranged by size: 

                1423    1117     601
 
SacII CC_GC'GG

Cuts at:      0     119    3141 
   Size:        119    3022
 
SanDI GG'GwC_CC

Cuts at:      0    2111    3141 
   Size:       2111    1030
 
ScaI AGT'ACT

Cuts at:      0    1346    3141 
   Size:       1346    1795
 
SfcI C'TryA_G

Cuts at:      0    1113    1714    2948    3141 
   Size:       1113     601    1234     193

  Fragments arranged by size: 

                1234    1113     601     193
 
SmlI C'TyrA_G

Cuts at:      0    1309    3141 
   Size:       1309    1832
 
SphI G_CATG'C

Cuts at:      0    2053    3141 
   Size:       2053    1088
 
SspI AAT'ATT

Cuts at:      0    1185    2576    3141 
   Size:       1185    1391     565

  Fragments arranged by size: 

                1391    1185     565
 
StuI AGG'CCT

Cuts at:      0     986    3141 
   Size:        986    2155
 
StyI C'CwwG_G

Cuts at:      0     213    1056    3141 
   Size:        213     843    2085

  Fragments arranged by size: 

                2085     843     213
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     172    2258    2612    3141 
   Size:        172    2086     354     529

  Fragments arranged by size: 

                2086     529     354     172
 
TatI w'GTAC_w

Cuts at:      0     648    1344    2165    3141 
   Size:        648     696     821     976

  Fragments arranged by size: 

                 976     821     696     648
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1151    1183    3141 
   Size:       1151      32    1958

  Fragments arranged by size: 

                1958    1151      32
 
XbaI T'CTAG_A

Cuts at:      0    2959    3141 
   Size:       2959     182
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     536    3141 
   Size:        536    2605
 
XmnI GAAnn'nnTTC

Cuts at:      0    1184    2066    2575    2594    3095    3141 
   Size:       1184     882     509      19     501      46

  Fragments arranged by size: 

                1184     882     509     501      46      19
 

 Enzymes that do cut:

     AccI     AclI     AcuI    AflII   AflIII     AhdI     AleI    AlwNI
    ApaLI     ApoI     AseI     AvaI    AvrII     BaeI    BanII     BbsI
     BclI    BglII     BlpI Bme1580I     BmrI     BpmI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BstBI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI    CspCI     DraI
     DrdI     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I
    EcoRV     FalI    Hin4I   HincII  HindIII     MmeI     MscI     MslI
   MspA1I     NaeI     NcoI   NgoMIV     NspI     PciI     PpiI    PpuMI
     PsiI     PstI    SacII    SanDI     ScaI     SfcI     SmlI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI     XbaI     XcmI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI     AgeI     AlfI     AloI     ApaI
     AscI    AsiSI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI
    BfrBI     BglI    BmgBI     BmtI     BplI    BpuEI    BsaHI    BseYI
    BsiWI    BsmBI    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI
    BtgZI     ClaI   DraIII  EcoICRI    EcoRI     FseI     FspI    FspAI
    HaeII     HpaI     KasI     KpnI     MfeI     MluI     NarI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmeI     PmlI    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SwaI  Tth111I
     XhoI     XmaI     ZraI
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