(Linear) (Six-base) MAPSORT of: KIEE4237.seq Check: 8856 from: 1 to: 3141 KIEE4237.orf With 182 enzymes: SgfI * December 5, 2014 12:57 .. AccI GT'mk_AC Cuts at: 0 3118 3141 Size: 3118 23 AclI AA'CG_TT Cuts at: 0 1464 3141 Size: 1464 1677 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2570 3141 Size: 2570 571 AflII C'TTAA_G Cuts at: 0 1309 3141 Size: 1309 1832 AflIII A'CryG_T Cuts at: 0 2226 3141 Size: 2226 915 AhdI GACnn_n'nnGTC Cuts at: 0 1103 3141 Size: 1103 2038 AleI CACnn'nnGTG Cuts at: 0 1643 3141 Size: 1643 1498 AlwNI CAG_nnn'CTG Cuts at: 0 886 3141 Size: 886 2255 ApaLI G'TGCA_C Cuts at: 0 3050 3141 Size: 3050 91 ApoI r'AATT_y Cuts at: 0 963 1473 2173 2833 3141 Size: 963 510 700 660 308 Fragments arranged by size: 963 700 660 510 308 AseI AT'TA_AT Cuts at: 0 1555 3141 Size: 1555 1586 AvaI C'yCGr_G Cuts at: 0 382 448 3141 Size: 382 66 2693 Fragments arranged by size: 2693 382 66 AvrII C'CTAG_G Cuts at: 0 213 3141 Size: 213 2928 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 860 893 3141 Size: 860 33 2248 Fragments arranged by size: 2248 860 33 BanII G_rGCy'C Cuts at: 0 79 2852 3141 Size: 79 2773 289 Fragments arranged by size: 2773 289 79 BbsI GAAGACnn'nnnn_ Cuts at: 0 735 1004 3141 Size: 735 269 2137 Fragments arranged by size: 2137 735 269 BclI T'GATC_A Cuts at: 0 2900 3141 Size: 2900 241 BglII A'GATC_T Cuts at: 0 1560 3141 Size: 1560 1581 BlpI GC'TnA_GC Cuts at: 0 2074 3141 Size: 2074 1067 Bme1580I G_kGCm'C Cuts at: 0 240 3054 3141 Size: 240 2814 87 Fragments arranged by size: 2814 240 87 BmrI ACTGGGnnnn_n' Cuts at: 0 250 805 1813 1900 3141 Size: 250 555 1008 87 1241 Fragments arranged by size: 1241 1008 555 250 87 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 63 74 1667 3141 Size: 63 11 1593 1474 Fragments arranged by size: 1593 1474 63 11 Bpu10I CC'TnA_GC Cuts at: 0 2843 3141 Size: 2843 298 BsaI GGTCTCn'nnnn_ Cuts at: 0 154 3141 Size: 154 2987 BsaAI yAC'GTr Cuts at: 0 3063 3141 Size: 3063 78 BsaBI GATnn'nnATC Cuts at: 0 1274 3141 Size: 1274 1867 BsaWI w'CCGG_w Cuts at: 0 163 1169 2940 3141 Size: 163 1006 1771 201 Fragments arranged by size: 1771 1006 201 163 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 510 540 3141 Size: 510 30 2601 Fragments arranged by size: 2601 510 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 40 71 508 515 3141 Size: 40 31 437 7 2626 Fragments arranged by size: 2626 437 40 31 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2830 3141 Size: 2830 311 BsiEI CG_ry'CG Cuts at: 0 11 3141 Size: 11 3130 BsiHKAI G_wGCw'C Cuts at: 0 2130 3054 3141 Size: 2130 924 87 Fragments arranged by size: 2130 924 87 BsmI GAATG_Cn' Cuts at: 0 2955 3141 Size: 2955 186 Bsp1286I G_dGCh'C Cuts at: 0 79 240 2130 2852 3054 3141 Size: 79 161 1890 722 202 87 Fragments arranged by size: 1890 722 202 161 87 79 BspEI T'CCGG_A Cuts at: 0 1169 2940 3141 Size: 1169 1771 201 Fragments arranged by size: 1771 1169 201 BspHI T'CATG_A Cuts at: 0 1590 3141 Size: 1590 1551 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 434 3141 Size: 434 2707 BsrBI CCG'CTC Cuts at: 0 523 3141 Size: 523 2618 BsrDI GCAATG_nn' Cuts at: 0 2989 3141 Size: 2989 152 BsrFI r'CCGG_y Cuts at: 0 16 307 1238 3141 Size: 16 291 931 1903 Fragments arranged by size: 1903 931 291 16 BstBI TT'CG_AA Cuts at: 0 3027 3141 Size: 3027 114 BstYI r'GATC_y Cuts at: 0 1029 1560 1998 3141 Size: 1029 531 438 1143 Fragments arranged by size: 1143 1029 531 438 BstZ17I GTA'TAC Cuts at: 0 3119 3141 Size: 3119 22 Bsu36I CC'TnA_GG Cuts at: 0 583 3141 Size: 583 2558 BtgI C'CryG_G Cuts at: 0 116 1056 2734 3141 Size: 116 940 1678 407 Fragments arranged by size: 1678 940 407 116 BtsI GCAGTG_nn' Cuts at: 0 27 101 2411 3141 Size: 27 74 2310 730 Fragments arranged by size: 2310 730 74 27 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2358 2393 2588 2623 2810 2845 3141 Size: 2358 35 195 35 187 35 296 Fragments arranged by size: 2358 296 195 187 35 35 35 DraI TTT'AAA Cuts at: 0 1197 2178 2456 3141 Size: 1197 981 278 685 Fragments arranged by size: 1197 981 685 278 DrdI GACnn_nn'nnGTC Cuts at: 0 2714 3141 Size: 2714 427 EaeI y'GGCC_r Cuts at: 0 8 305 506 2255 3141 Size: 8 297 201 1749 886 Fragments arranged by size: 1749 886 297 201 8 EagI C'GGCC_G Cuts at: 0 8 3141 Size: 8 3133 EarI CTCTTCn'nnn_ Cuts at: 0 494 1004 1913 2156 3141 Size: 494 510 909 243 985 Fragments arranged by size: 985 909 510 494 243 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 203 3141 Size: 203 2938 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 63 74 1667 2570 3141 Size: 63 11 1593 903 571 Fragments arranged by size: 1593 903 571 63 11 EcoNI CCTnn'n_nnAGG Cuts at: 0 586 1771 3141 Size: 586 1185 1370 Fragments arranged by size: 1370 1185 586 EcoO109I rG'GnC_Cy Cuts at: 0 641 1130 2111 3141 Size: 641 489 981 1030 Fragments arranged by size: 1030 981 641 489 EcoRV GAT'ATC Cuts at: 0 2007 3141 Size: 2007 1134 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1425 1456 3141 Size: 1425 31 1685 Fragments arranged by size: 1685 1425 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 510 542 1089 1121 1695 1727 2749 2781 Size: 510 32 547 32 574 32 1022 32 Cuts at: 2781 3141 Size: 360 Fragments arranged by size: 1022 574 547 510 360 32 32 32 32 HincII GTy'rAC Cuts at: 0 518 681 3141 Size: 518 163 2460 Fragments arranged by size: 2460 518 163 HindIII A'AGCT_T Cuts at: 0 1079 2290 3073 3141 Size: 1079 1211 783 68 Fragments arranged by size: 1211 1079 783 68 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 553 1231 1447 3141 Size: 553 678 216 1694 Fragments arranged by size: 1694 678 553 216 MscI TGG'CCA Cuts at: 0 508 2257 3141 Size: 508 1749 884 Fragments arranged by size: 1749 884 508 MslI CAynn'nnrTG Cuts at: 0 1643 2229 2231 2389 3017 3141 Size: 1643 586 2 158 628 124 Fragments arranged by size: 1643 628 586 158 124 2 MspA1I CmG'CkG Cuts at: 0 73 118 3141 Size: 73 45 3023 Fragments arranged by size: 3023 73 45 NaeI GCC'GGC Cuts at: 0 18 3141 Size: 18 3123 NcoI C'CATG_G Cuts at: 0 1056 3141 Size: 1056 2085 NgoMIV G'CCGG_C Cuts at: 0 16 3141 Size: 16 3125 NspI r_CATG'y Cuts at: 0 2053 2230 3141 Size: 2053 177 911 Fragments arranged by size: 2053 911 177 PciI A'CATG_T Cuts at: 0 2226 3141 Size: 2226 915 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1331 1363 3141 Size: 1331 32 1778 Fragments arranged by size: 1778 1331 32 PpuMI rG'GwC_Cy Cuts at: 0 641 2111 3141 Size: 641 1470 1030 Fragments arranged by size: 1470 1030 641 PsiI TTA'TAA Cuts at: 0 616 1085 1247 1415 3141 Size: 616 469 162 168 1726 Fragments arranged by size: 1726 616 469 168 162 PstI C_TGCA'G Cuts at: 0 1117 1718 3141 Size: 1117 601 1423 Fragments arranged by size: 1423 1117 601 SacII CC_GC'GG Cuts at: 0 119 3141 Size: 119 3022 SanDI GG'GwC_CC Cuts at: 0 2111 3141 Size: 2111 1030 ScaI AGT'ACT Cuts at: 0 1346 3141 Size: 1346 1795 SfcI C'TryA_G Cuts at: 0 1113 1714 2948 3141 Size: 1113 601 1234 193 Fragments arranged by size: 1234 1113 601 193 SmlI C'TyrA_G Cuts at: 0 1309 3141 Size: 1309 1832 SphI G_CATG'C Cuts at: 0 2053 3141 Size: 2053 1088 SspI AAT'ATT Cuts at: 0 1185 2576 3141 Size: 1185 1391 565 Fragments arranged by size: 1391 1185 565 StuI AGG'CCT Cuts at: 0 986 3141 Size: 986 2155 StyI C'CwwG_G Cuts at: 0 213 1056 3141 Size: 213 843 2085 Fragments arranged by size: 2085 843 213 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 172 2258 2612 3141 Size: 172 2086 354 529 Fragments arranged by size: 2086 529 354 172 TatI w'GTAC_w Cuts at: 0 648 1344 2165 3141 Size: 648 696 821 976 Fragments arranged by size: 976 821 696 648 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1151 1183 3141 Size: 1151 32 1958 Fragments arranged by size: 1958 1151 32 XbaI T'CTAG_A Cuts at: 0 2959 3141 Size: 2959 182 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 536 3141 Size: 536 2605 XmnI GAAnn'nnTTC Cuts at: 0 1184 2066 2575 2594 3095 3141 Size: 1184 882 509 19 501 46 Fragments arranged by size: 1184 882 509 501 46 19 Enzymes that do cut: AccI AclI AcuI AflII AflIII AhdI AleI AlwNI ApaLI ApoI AseI AvaI AvrII BaeI BanII BbsI BclI BglII BlpI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BstBI BstYI BstZ17I Bsu36I BtgI BtsI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRV FalI Hin4I HincII HindIII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PciI PpiI PpuMI PsiI PstI SacII SanDI ScaI SfcI SmlI SphI SspI StuI StyI TaqII TatI TstI XbaI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AfeI AgeI AlfI AloI ApaI AscI AsiSI BamHI BanI BbeI BbvCI BcgI BciVI BfrBI BglI BmgBI BmtI BplI BpuEI BsaHI BseYI BsiWI BsmBI BsrGI BssHII BssSI BstAPI BstEII BstXI BtgZI ClaI DraIII EcoICRI EcoRI FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmeI PmlI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SalI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI Tth111I XhoI XmaI ZraI