################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BbvI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BsaHI BsaI BsaWI BseAI BseRI BsgI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstXI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI Ksp632I MaeI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TseI Tsp45I Tsp509I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### Acc65I 242 GGTACC G^GTACC AflIII 101 ACGTGT A^C[GA][CT]GT AluI 318 AGCT AG^CT ApaI 586 GGGCCC GGGCC^C Asp718I 242 GGTACC G^GTACC AspHI 317 GAGCTC G[AT]GC[AT]^C AvaI 323 CCCGAG C^[CT]CG[GA]G BanI 242 GGTACC G^G[CT][GA]CC BbsI 202 GAAGAC GAAGAC(2/6) BcgI 467 GCACTGGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 570 CCGGG CC^[GC]GG BglI 287 GCCGAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 71 CTCCAG CTGGAG(16/14) BpuAI 202 GAAGAC GAAGAC(2/6) BsaAI 55 TACGTG [CT]AC^GT[GA] BsaBI 611 GATCTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 273 CGGCCG CG[GA][CT]^CG BsiHKAI 317 GAGCTC G[AT]GC[AT]^C BsmBI 194 GAGACG CGTCTC(1/5) BsmFI 156 GGGAC GGGAC(10/14) BsoBI 323 CCCGAG C^[CT]CG[GA]G BspHI 546 TCATGA T^CATGA BspMI 430 GCAGGT ACCTGC(4/8) BstBI 515 TTCGAA TT^CGAA BstNI 347 CCAGG CC^[AT]GG CfoI 119 GCGC GCG^C DdeI 533 CTAAG C^T[ACGT]AG DraI 408 TTTAAA TTT^AAA DraII 185 AGGACCC [GA]G^G[ACGT]CC[CT] EaeI 273 CGGCCG [CT]^GGCC[GA] EagI 273 CGGCCG C^GGCCG EclXI 273 CGGCCG C^GGCCG Eco52I 273 CGGCCG C^GGCCG EcoO109I 185 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRII 347 CCAGG ^CC[AT]GG HaeII 118 GGCGCT [GA]GCGC^[CT] HapII 570 CCGG C^CGG HhaI 119 GCGC GCG^C HinP1I 119 GCGC G^CGC HpaII 570 CCGG C^CGG KpnI 242 GGTACC GGTAC^C KspI 150 CCGCGG CCGC^GG MamI 611 GATCTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 150 CCGCGG C[AC]G^C[GT]G MspI 570 CCGG C^CGG MvaI 347 CCAGG CC^[AT]GG NciI 570 CCGGG CC^[GC]GG NruI 219 TCGCGA TCG^CGA NspI 419 ACATGC [GA]CATG^[CT] NspV 515 TTCGAA TT^CGAA PflMI 598 CCAAGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 666 GAGTC GAGTC(4/5) PpuMI 185 AGGACCC [GA]G^G[AT]CC[CT] RcaI 546 TCATGA T^CATGA SacI 317 GAGCTC GAGCT^C SacII 150 CCGCGG CCGC^GG SfaNI 50 GCATC GCATC(5/9) SfuI 515 TTCGAA TT^CGAA SmlI 139 CTCAAG C^T[CT][GA]AG TspRI 468 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 598 CCAAGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 388 CCAAGTGGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwI 414 GGATC GGATC(4/5) 697 GGATC AvaII 157 GGACC G^G[AT]CC 186 GGACC BanII 317 GAGCTC G[GA]GC[CT]^C 586 GGGCCC BmyI 317 GAGCTC G[AGT]GC[ACT]^C 586 GGGCCC BsmAI 194 GAGAC GTCTC(1/5) 373 GTCTC Bsp1286I 317 GAGCTC G[AGT]GC[ACT]^C 586 GGGCCC BsrI 469 ACTGG ACTGG(1/-1) 522 ACTGG BssKI 347 CCAGG ^CC[ACGT]GG 570 CCGGG DsaI 150 CCGCGG C^C[GA][CT]GG 276 CCGTGG EcoT14I 86 CCAAGG C^C[AT][AT]GG 682 CCAAGG HgaI 196 GACGC GACGC(5/10) 205 GACGC HphI 433 GGTGA GGTGA(8/7) 700 TCACC MaeII 56 ACGT A^CGT 101 ACGT MaeIII 62 GTAAC ^GT[ACGT]AC 674 GTTAC MseI 409 TTAA T^TAA 575 TTAA MslI 51 CATCTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 418 CACATGCGTG NlaIII 420 CATG CATG^ 547 CATG ScrFI 347 CCAGG CC^[ACGT]GG 570 CCGGG StyI 86 CCAAGG C^C[AT][AT]GG 682 CCAAGG TfiI 18 GATTC G^A[AT]TC 144 GAATC Tru9I 409 TTAA T^TAA 575 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AccII AciI AfaI BglII BsaJI BsiYI BslI BstF5I BstUI BstYI Cfr13I DpnII FauI Fnu4HI FokI HaeIII HinfI ItaI MboI MboII MflI MnlI MvnI MwoI NdeII NlaIV RsaI Sau3AI Sau96I TaqI TthHB8I XhoII