(Circular) (Six-base) MAPSORT of: pFN21AE5280.seq Check: 1355 from: 1 to: 5449 pFN21AE5280 5449 bp With 182 enzymes: SgfI * January 30, 2014 17:16 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2426 2426 Size: 5449 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3259 278 Size: 53 83 186 936 1723 2468 Fragments arranged by size: 2468 1723 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2792 1937 Size: 855 4594 Acc65I G'GTAC_C Cuts at: 2247 2770 2247 Size: 523 4926 AclI AA'CG_TT Cuts at: 1563 3576 3949 1563 Size: 2013 373 3063 Fragments arranged by size: 3063 2013 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3512 4896 5164 1632 Size: 1880 1384 268 1917 Fragments arranged by size: 1917 1880 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5252 AflIII A'CryG_T Cuts at: 1203 2106 4348 5325 1203 Size: 903 2242 977 1327 Fragments arranged by size: 2242 1327 977 903 AgeI A'CCGG_T Cuts at: 4280 4280 Size: 5449 AhdI GACnn_n'nnGTC Cuts at: 4178 4178 Size: 5449 AleI CACnn'nnGTG Cuts at: 1166 2427 1166 Size: 1261 4188 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5449 AlwNI CAG_nnn'CTG Cuts at: 3192 4764 3192 Size: 1572 3877 ApaI G_GGCC'C Cuts at: 2595 2595 Size: 5449 ApaLI G'TGCA_C Cuts at: 3506 4662 3506 Size: 1156 4293 ApoI r'AATT_y Cuts at: 1493 2758 2984 3136 5245 1493 Size: 1265 226 152 2109 1697 Fragments arranged by size: 2109 1697 1265 226 152 AseI AT'TA_AT Cuts at: 160 1032 4003 160 Size: 872 2971 1606 Fragments arranged by size: 2971 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5449 AvaI C'yCGr_G Cuts at: 1870 1943 2328 2774 1870 Size: 73 385 446 4545 Fragments arranged by size: 4545 446 385 73 BamHI G'GATC_C Cuts at: 2779 3146 2779 Size: 367 5082 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2247 2770 4225 618 Size: 325 217 627 460 523 1455 1842 Fragments arranged by size: 1842 1455 627 523 460 325 217 BanII G_rGCy'C Cuts at: 1391 2326 2595 2768 1391 Size: 935 269 173 4072 Fragments arranged by size: 4072 935 269 173 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2214 928 Size: 971 315 4163 Fragments arranged by size: 4163 971 315 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2461 2495 3640 3674 1903 Size: 34 524 34 1145 34 3678 Fragments arranged by size: 3678 1145 524 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3341 4551 3341 Size: 1210 4239 BclI T'GATC_A Cuts at: 1548 1548 Size: 5449 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2298 4060 136 Size: 107 122 71 631 605 626 1762 1525 Fragments arranged by size: 1762 1525 631 626 605 122 107 71 BglII A'GATC_T Cuts at: 2546 2615 2732 5444 2546 Size: 69 117 2712 2551 Fragments arranged by size: 2712 2551 117 69 BlpI GC'TnA_GC Cuts at: 2866 2866 Size: 5449 Bme1580I G_kGCm'C Cuts at: 1790 2595 3510 4666 1790 Size: 805 915 1156 2573 Fragments arranged by size: 2573 1156 915 805 BmgBI CAC'GTC Cuts at: 1318 2107 1318 Size: 789 4660 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3180 4138 442 Size: 416 53 83 399 24 1763 958 1753 Fragments arranged by size: 1763 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5449 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2061 4109 1239 Size: 822 2048 2579 Fragments arranged by size: 2579 2048 822 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2129 3574 4439 4737 2129 Size: 1445 865 298 2841 Fragments arranged by size: 2841 1445 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4112 882 Size: 617 2613 2219 Fragments arranged by size: 2613 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2062 3240 493 Size: 711 858 1178 2702 Fragments arranged by size: 2702 1178 858 711 BsaBI GATnn'nnATC Cuts at: 2620 5443 2620 Size: 2823 2626 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3256 3638 4273 275 Size: 53 83 186 936 1723 382 635 1451 Fragments arranged by size: 1723 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3882 4280 4554 4701 5112 3882 Size: 398 274 147 411 4219 Fragments arranged by size: 4219 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2004 2034 2662 2692 1178 Size: 30 165 30 601 30 628 30 3935 Fragments arranged by size: 3935 628 601 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5449 BseYI C'CCAG_C Cuts at: 4652 5148 4652 Size: 496 4953 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4876 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2281 2899 3660 4688 1540 Size: 275 186 280 618 761 1028 2301 Fragments arranged by size: 2301 1028 761 618 280 275 186 BsiHKAI G_wGCw'C Cuts at: 2326 3510 3595 4666 2326 Size: 1184 85 1071 3109 Fragments arranged by size: 3109 1184 1071 85 BsmI GAATG_Cn' Cuts at: 2960 3053 2960 Size: 93 5356 BsmBI CGTCTCn'nnnn_ Cuts at: 2193 2193 Size: 5449 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2326 2595 2768 3510 3595 4666 1391 Size: 399 536 269 173 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 536 399 269 173 85 BspEI T'CCGG_A Cuts at: 5112 5112 Size: 5449 BspHI T'CATG_A Cuts at: 2551 3338 5068 2551 Size: 787 1730 2932 Fragments arranged by size: 2932 1730 787 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2426 2804 844 Size: 1582 378 3489 Fragments arranged by size: 3489 1582 378 BsrBI CCG'CTC Cuts at: 1394 1823 3336 1394 Size: 429 1513 3507 Fragments arranged by size: 3507 1513 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3944 4118 65 Size: 1183 2696 174 1396 Fragments arranged by size: 2696 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4093 4280 1700 Size: 110 2283 187 2869 Fragments arranged by size: 2869 2283 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5449 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5449 BssSI C'ACGA_G Cuts at: 3509 4521 3509 Size: 1012 4437 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5449 BstBI TT'CG_AA Cuts at: 2521 4253 2521 Size: 1732 3717 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5449 BstYI r'GATC_y Cuts at: 1972 2546 2615 2732 2779 3146 3531 3548 5000 Size: 574 69 117 47 367 385 17 1452 Cuts at: 5000 5086 5098 5444 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 574 385 367 346 117 86 69 47 17 12 BtgI C'CryG_G Cuts at: 513 1065 2155 2281 513 Size: 552 1090 126 3681 Fragments arranged by size: 3681 1090 552 126 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4710 Fragments arranged by size: 4710 683 56 BtsI GCAGTG_nn' Cuts at: 2965 3759 3779 2965 Size: 794 20 4635 Fragments arranged by size: 4635 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5449 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5414 DraI TTT'AAA Cuts at: 2415 2753 2415 Size: 338 5111 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4456 809 Size: 507 3140 1802 Fragments arranged by size: 3140 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2278 2896 3785 Size: 54 1425 50 275 18 448 618 889 Cuts at: 3785 5414 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 618 448 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2278 2896 1537 Size: 275 466 618 4090 Fragments arranged by size: 4090 618 466 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3379 1353 Size: 2026 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2334 4036 4410 4556 387 Size: 1064 138 745 1702 374 146 1280 Fragments arranged by size: 1702 1280 1064 745 374 146 138 EcoICRI GAG'CTC Cuts at: 2324 2324 Size: 5449 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2061 3512 4109 4896 5164 1239 Size: 393 429 1451 597 787 268 1524 Fragments arranged by size: 1524 1451 787 597 429 393 268 EcoO109I rG'GnC_Cy Cuts at: 2191 2191 Size: 5449 EcoRI G'AATT_C Cuts at: 2758 3136 2758 Size: 378 5071 FspI TGC'GCA Cuts at: 3955 3955 Size: 5449 HaeII r_GCGC'y Cuts at: 1678 2127 4596 1678 Size: 449 2469 2531 Fragments arranged by size: 2531 2469 449 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2361 2393 4090 4122 4164 Size: 32 135 32 789 32 1697 32 42 Cuts at: 4164 4196 1373 Size: 32 2626 Fragments arranged by size: 2626 1697 789 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2793 3039 669 Size: 988 281 855 246 3079 Fragments arranged by size: 3079 988 855 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5449 HpaI GTT'AAC Cuts at: 3039 3039 Size: 5449 KpnI G_GTAC'C Cuts at: 2251 2774 2251 Size: 523 4926 MfeI C'AATT_G Cuts at: 3048 3048 Size: 5449 MluI A'CGCG_T Cuts at: 5325 5325 Size: 5449 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4563 4747 1305 Size: 3258 184 2007 Fragments arranged by size: 3258 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3627 Fragments arranged by size: 3627 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2060 2427 3407 3766 3925 518 Size: 648 894 367 980 359 159 2042 Fragments arranged by size: 2042 980 894 648 367 359 159 MspA1I CmG'CkG Cuts at: 1606 2157 2865 3542 4690 4935 1606 Size: 551 708 677 1148 245 2120 Fragments arranged by size: 2120 1148 708 677 551 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4897 NdeI CA'TA_TG Cuts at: 387 387 Size: 5449 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5449 NotI GC'GGCC_GC Cuts at: 2896 2896 Size: 5449 NruI TCG'CGA Cuts at: 2226 2226 Size: 5449 NspI r_CATG'y Cuts at: 1132 1229 2428 2807 4352 1132 Size: 97 1199 379 1545 2229 Fragments arranged by size: 2229 1545 1199 379 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5449 PciI A'CATG_T Cuts at: 4348 4348 Size: 5449 PflMI CCAn_nnn'nTGG Cuts at: 1108 2609 1108 Size: 1501 3948 PmeI GTTT'AAAC Cuts at: 2753 2753 Size: 5449 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2093 2125 3518 3550 5031 5063 2093 Size: 32 1393 32 1481 32 2479 Fragments arranged by size: 2479 1481 1393 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2191 2191 Size: 5449 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5449 PsiI TTA'TAA Cuts at: 3019 3019 Size: 5449 PspOMI G'GGCC_C Cuts at: 2591 2591 Size: 5449 PstI C_TGCA'G Cuts at: 830 2801 830 Size: 1971 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5449 SacI G_AGCT'C Cuts at: 2326 2326 Size: 5449 SacII CC_GC'GG Cuts at: 2158 2158 Size: 5449 SalI G'TCGA_C Cuts at: 1936 2791 1936 Size: 855 4594 SbfI CC_TGCA'GG Cuts at: 2801 2801 Size: 5449 ScaI AGT'ACT Cuts at: 3697 5409 3697 Size: 1712 3737 SfcI C'TryA_G Cuts at: 826 1045 2797 3932 4613 4804 826 Size: 219 1752 1135 681 191 1471 Fragments arranged by size: 1752 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5449 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5449 SmaI CCC'GGG Cuts at: 1872 2776 1872 Size: 904 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2144 3553 4454 4716 820 Size: 197 926 201 1409 901 262 1553 Fragments arranged by size: 1553 1409 926 901 262 201 197 SnaBI TAC'GTA Cuts at: 493 3240 493 Size: 2747 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5449 SphI G_CATG'C Cuts at: 1132 2807 1132 Size: 1675 3774 SspI AAT'ATT Cuts at: 5 52 3373 5 Size: 47 3321 2081 Fragments arranged by size: 3321 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5449 StyI C'CwwG_G Cuts at: 513 1065 2091 2687 2888 513 Size: 552 1026 596 201 3074 Fragments arranged by size: 3074 1026 596 552 201 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3476 3493 3646 3831 4276 325 Size: 1255 1896 17 153 185 445 1498 Fragments arranged by size: 1896 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3695 5407 96 Size: 274 80 33 51 1443 1718 1712 138 Fragments arranged by size: 1718 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2004 2036 5409 5441 2004 Size: 32 3373 32 2012 Fragments arranged by size: 3373 2012 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5449 XbaI T'CTAG_A Cuts at: 2785 2785 Size: 5449 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2400 2400 Size: 5449 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5449 XmaI C'CCGG_G Cuts at: 1870 2774 1870 Size: 904 4545 XmnI GAAnn'nnTTC Cuts at: 3578 3578 Size: 5449 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3257 276 Size: 53 83 186 936 1723 2468 Fragments arranged by size: 2468 1723 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI