Restriction map for pFN21AE5280
 (Circular) (Six-base) MAPSORT of: pFN21AE5280.seq  Check: 1355  from: 1  to: 5449



pFN21AE5280  5449 bp

 With 182 enzymes: SgfI * 

                        January 30, 2014 17:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2426    2426 
   Size:       5449
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3259     278 
   Size:         53      83     186     936    1723    2468

  Fragments arranged by size: 

                2468    1723     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2792    1937 
   Size:        855    4594
 
Acc65I G'GTAC_C

Cuts at:   2247    2770    2247 
   Size:        523    4926
 
AclI AA'CG_TT

Cuts at:   1563    3576    3949    1563 
   Size:       2013     373    3063

  Fragments arranged by size: 

                3063    2013     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3512    4896    5164    1632 
   Size:       1880    1384     268    1917

  Fragments arranged by size: 

                1917    1880    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5252
 
AflIII A'CryG_T

Cuts at:   1203    2106    4348    5325    1203 
   Size:        903    2242     977    1327

  Fragments arranged by size: 

                2242    1327     977     903
 
AgeI A'CCGG_T

Cuts at:   4280    4280 
   Size:       5449
 
AhdI GACnn_n'nnGTC

Cuts at:   4178    4178 
   Size:       5449
 
AleI CACnn'nnGTG

Cuts at:   1166    2427    1166 
   Size:       1261    4188
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5449
 
AlwNI CAG_nnn'CTG

Cuts at:   3192    4764    3192 
   Size:       1572    3877
 
ApaI G_GGCC'C

Cuts at:   2595    2595 
   Size:       5449
 
ApaLI G'TGCA_C

Cuts at:   3506    4662    3506 
   Size:       1156    4293
 
ApoI r'AATT_y

Cuts at:   1493    2758    2984    3136    5245    1493 
   Size:       1265     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1265     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4003     160 
   Size:        872    2971    1606

  Fragments arranged by size: 

                2971    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5449
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2328    2774    1870 
   Size:         73     385     446    4545

  Fragments arranged by size: 

                4545     446     385      73
 
BamHI G'GATC_C

Cuts at:   2779    3146    2779 
   Size:        367    5082
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2247    2770    4225     618 
   Size:        325     217     627     460     523    1455    1842

  Fragments arranged by size: 

                1842    1455     627     523     460     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2326    2595    2768    1391 
   Size:        935     269     173    4072

  Fragments arranged by size: 

                4072     935     269     173
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2214     928 
   Size:        971     315    4163

  Fragments arranged by size: 

                4163     971     315
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2461    2495    3640    3674    1903 
   Size:         34     524      34    1145      34    3678

  Fragments arranged by size: 

                3678    1145     524      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3341    4551    3341 
   Size:       1210    4239
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5449
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2298    4060     136 
   Size:        107     122      71     631     605     626    1762    1525

  Fragments arranged by size: 

                1762    1525     631     626     605     122     107      71
            
 
BglII A'GATC_T

Cuts at:   2546    2615    2732    5444    2546 
   Size:         69     117    2712    2551

  Fragments arranged by size: 

                2712    2551     117      69
 
BlpI GC'TnA_GC

Cuts at:   2866    2866 
   Size:       5449
 
Bme1580I G_kGCm'C

Cuts at:   1790    2595    3510    4666    1790 
   Size:        805     915    1156    2573

  Fragments arranged by size: 

                2573    1156     915     805
 
BmgBI CAC'GTC

Cuts at:   1318    2107    1318 
   Size:        789    4660
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3180    4138     442 
   Size:        416      53      83     399      24    1763     958    1753

  Fragments arranged by size: 

                1763    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5449
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2061    4109    1239 
   Size:        822    2048    2579

  Fragments arranged by size: 

                2579    2048     822
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2129    3574    4439    4737    2129 
   Size:       1445     865     298    2841

  Fragments arranged by size: 

                2841    1445     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4112     882 
   Size:        617    2613    2219

  Fragments arranged by size: 

                2613    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2062    3240     493 
   Size:        711     858    1178    2702

  Fragments arranged by size: 

                2702    1178     858     711
 
BsaBI GATnn'nnATC

Cuts at:   2620    5443    2620 
   Size:       2823    2626
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3256    3638    4273     275 
   Size:         53      83     186     936    1723     382     635    1451

  Fragments arranged by size: 

                1723    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3882    4280    4554    4701    5112    3882 
   Size:        398     274     147     411    4219

  Fragments arranged by size: 

                4219     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2004    2034    2662    2692    1178 
   Size:         30     165      30     601      30     628      30    3935

  Fragments arranged by size: 

                3935     628     601     165      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5449
 
BseYI C'CCAG_C

Cuts at:   4652    5148    4652 
   Size:        496    4953
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4876
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2281    2899    3660    4688    1540 
   Size:        275     186     280     618     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     618     280     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2326    3510    3595    4666    2326 
   Size:       1184      85    1071    3109

  Fragments arranged by size: 

                3109    1184    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2960    3053    2960 
   Size:         93    5356
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2193    2193 
   Size:       5449
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2326    2595    2768    3510    3595    4666    1391 
   Size:        399     536     269     173     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     536     399     269     173      85
            
 
BspEI T'CCGG_A

Cuts at:   5112    5112 
   Size:       5449
 
BspHI T'CATG_A

Cuts at:   2551    3338    5068    2551 
   Size:        787    1730    2932

  Fragments arranged by size: 

                2932    1730     787
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2426    2804     844 
   Size:       1582     378    3489

  Fragments arranged by size: 

                3489    1582     378
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3336    1394 
   Size:        429    1513    3507

  Fragments arranged by size: 

                3507    1513     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3944    4118      65 
   Size:       1183    2696     174    1396

  Fragments arranged by size: 

                2696    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4093    4280    1700 
   Size:        110    2283     187    2869

  Fragments arranged by size: 

                2869    2283     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5449
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5449
 
BssSI C'ACGA_G

Cuts at:   3509    4521    3509 
   Size:       1012    4437
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5449
 
BstBI TT'CG_AA

Cuts at:   2521    4253    2521 
   Size:       1732    3717
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5449
 
BstYI r'GATC_y

Cuts at:   1972    2546    2615    2732    2779    3146    3531    3548    5000 
   Size:        574      69     117      47     367     385      17    1452

Cuts at:   5000    5086    5098    5444    1972 
   Size:         86      12     346    1977

  Fragments arranged by size: 

                1977    1452     574     385     367     346     117      86
                  69      47      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2155    2281     513 
   Size:        552    1090     126    3681

  Fragments arranged by size: 

                3681    1090     552     126
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4710

  Fragments arranged by size: 

                4710     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2965    3759    3779    2965 
   Size:        794      20    4635

  Fragments arranged by size: 

                4635     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5449
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5414
 
DraI TTT'AAA

Cuts at:   2415    2753    2415 
   Size:        338    5111
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4456     809 
   Size:        507    3140    1802

  Fragments arranged by size: 

                3140    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2278    2896    3785 
   Size:         54    1425      50     275      18     448     618     889

Cuts at:   3785    5414       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     618     448     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2278    2896    1537 
   Size:        275     466     618    4090

  Fragments arranged by size: 

                4090     618     466     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3379    1353 
   Size:       2026    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2334    4036    4410    4556     387 
   Size:       1064     138     745    1702     374     146    1280

  Fragments arranged by size: 

                1702    1280    1064     745     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2324    2324 
   Size:       5449
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2061    3512    4109    4896    5164    1239 
   Size:        393     429    1451     597     787     268    1524

  Fragments arranged by size: 

                1524    1451     787     597     429     393     268
 
EcoO109I rG'GnC_Cy

Cuts at:   2191    2191 
   Size:       5449
 
EcoRI G'AATT_C

Cuts at:   2758    3136    2758 
   Size:        378    5071
 
FspI TGC'GCA

Cuts at:   3955    3955 
   Size:       5449
 
HaeII r_GCGC'y

Cuts at:   1678    2127    4596    1678 
   Size:        449    2469    2531

  Fragments arranged by size: 

                2531    2469     449
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2361    2393    4090    4122    4164 
   Size:         32     135      32     789      32    1697      32      42

Cuts at:   4164    4196    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1697     789     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2793    3039     669 
   Size:        988     281     855     246    3079

  Fragments arranged by size: 

                3079     988     855     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5449
 
HpaI GTT'AAC

Cuts at:   3039    3039 
   Size:       5449
 
KpnI G_GTAC'C

Cuts at:   2251    2774    2251 
   Size:        523    4926
 
MfeI C'AATT_G

Cuts at:   3048    3048 
   Size:       5449
 
MluI A'CGCG_T

Cuts at:   5325    5325 
   Size:       5449
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4563    4747    1305 
   Size:       3258     184    2007

  Fragments arranged by size: 

                3258    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3627

  Fragments arranged by size: 

                3627    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2060    2427    3407    3766    3925     518 
   Size:        648     894     367     980     359     159    2042

  Fragments arranged by size: 

                2042     980     894     648     367     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2157    2865    3542    4690    4935    1606 
   Size:        551     708     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     708     677     551     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4897
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5449
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5449
 
NotI GC'GGCC_GC

Cuts at:   2896    2896 
   Size:       5449
 
NruI TCG'CGA

Cuts at:   2226    2226 
   Size:       5449
 
NspI r_CATG'y

Cuts at:   1132    1229    2428    2807    4352    1132 
   Size:         97    1199     379    1545    2229

  Fragments arranged by size: 

                2229    1545    1199     379      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5449
 
PciI A'CATG_T

Cuts at:   4348    4348 
   Size:       5449
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2609    1108 
   Size:       1501    3948
 
PmeI GTTT'AAAC

Cuts at:   2753    2753 
   Size:       5449
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2093    2125    3518    3550    5031    5063    2093 
   Size:         32    1393      32    1481      32    2479

  Fragments arranged by size: 

                2479    1481    1393      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2191    2191 
   Size:       5449
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5449
 
PsiI TTA'TAA

Cuts at:   3019    3019 
   Size:       5449
 
PspOMI G'GGCC_C

Cuts at:   2591    2591 
   Size:       5449
 
PstI C_TGCA'G

Cuts at:    830    2801     830 
   Size:       1971    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5449
 
SacI G_AGCT'C

Cuts at:   2326    2326 
   Size:       5449
 
SacII CC_GC'GG

Cuts at:   2158    2158 
   Size:       5449
 
SalI G'TCGA_C

Cuts at:   1936    2791    1936 
   Size:        855    4594
 
SbfI CC_TGCA'GG

Cuts at:   2801    2801 
   Size:       5449
 
ScaI AGT'ACT

Cuts at:   3697    5409    3697 
   Size:       1712    3737
 
SfcI C'TryA_G

Cuts at:    826    1045    2797    3932    4613    4804     826 
   Size:        219    1752    1135     681     191    1471

  Fragments arranged by size: 

                1752    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5449
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5449
 
SmaI CCC'GGG

Cuts at:   1872    2776    1872 
   Size:        904    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2144    3553    4454    4716     820 
   Size:        197     926     201    1409     901     262    1553

  Fragments arranged by size: 

                1553    1409     926     901     262     201     197
 
SnaBI TAC'GTA

Cuts at:    493    3240     493 
   Size:       2747    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5449
 
SphI G_CATG'C

Cuts at:   1132    2807    1132 
   Size:       1675    3774
 
SspI AAT'ATT

Cuts at:      5      52    3373       5 
   Size:         47    3321    2081

  Fragments arranged by size: 

                3321    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5449
 
StyI C'CwwG_G

Cuts at:    513    1065    2091    2687    2888     513 
   Size:        552    1026     596     201    3074

  Fragments arranged by size: 

                3074    1026     596     552     201
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3476    3493    3646    3831    4276     325 
   Size:       1255    1896      17     153     185     445    1498

  Fragments arranged by size: 

                1896    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3695    5407      96 
   Size:        274      80      33      51    1443    1718    1712     138

  Fragments arranged by size: 

                1718    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2004    2036    5409    5441    2004 
   Size:         32    3373      32    2012

  Fragments arranged by size: 

                3373    2012      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5449
 
XbaI T'CTAG_A

Cuts at:   2785    2785 
   Size:       5449
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2400    2400 
   Size:       5449
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5449
 
XmaI C'CCGG_G

Cuts at:   1870    2774    1870 
   Size:        904    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3578    3578 
   Size:       5449
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3257     276 
   Size:         53      83     186     936    1723    2468

  Fragments arranged by size: 

                2468    1723     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NruI     NspI
     PasI     PciI    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI
   PspOMI     PstI     PvuI     SacI    SacII     SalI     SbfI     ScaI
     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     AscI    AvrII     BaeI     BbeI    BbvCI    BfrBI
     BplI   Bpu10I    BsiWI    BstXI  BstZ17I   Bsu36I   DraIII    EcoNI
    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NsiI     PacI     PfoI     PmlI    PspXI     PsrI    PvuII    RsrII
    SanDI     SapI    SexAI     SfiI     SfoI     SrfI     SwaI
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