(Linear) (Six-base) MAPSORT of: KIEE5280.seq Check: 1390 from: 1 to: 744 KIEE5280.orf With 182 enzymes: SgfI * January 29, 2014 11:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 421 744 Size: 421 323 Acc65I G'GTAC_C Cuts at: 0 242 744 Size: 242 502 AflIII A'CryG_T Cuts at: 0 101 744 Size: 101 643 AleI CACnn'nnGTG Cuts at: 0 422 744 Size: 422 322 ApaI G_GGCC'C Cuts at: 0 590 744 Size: 590 154 AvaI C'yCGr_G Cuts at: 0 323 744 Size: 323 421 BanI G'GyrC_C Cuts at: 0 242 744 Size: 242 502 BanII G_rGCy'C Cuts at: 0 321 590 744 Size: 321 269 154 Fragments arranged by size: 321 269 154 BbsI GAAGACnn'nnnn_ Cuts at: 0 209 744 Size: 209 535 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 456 490 744 Size: 456 34 254 Fragments arranged by size: 456 254 34 BglI GCCn_nnn'nGGC Cuts at: 0 293 744 Size: 293 451 BglII A'GATC_T Cuts at: 0 541 610 727 744 Size: 541 69 117 17 Fragments arranged by size: 541 117 69 17 Bme1580I G_kGCm'C Cuts at: 0 590 744 Size: 590 154 BmgBI CAC'GTC Cuts at: 0 102 744 Size: 102 642 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 56 744 Size: 56 688 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 744 Size: 124 620 BsaAI yAC'GTr Cuts at: 0 57 744 Size: 57 687 BsaBI GATnn'nnATC Cuts at: 0 615 744 Size: 615 129 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 29 657 687 744 Size: 29 628 30 57 Fragments arranged by size: 628 57 30 29 BsiEI CG_ry'CG Cuts at: 0 276 744 Size: 276 468 BsiHKAI G_wGCw'C Cuts at: 0 321 744 Size: 321 423 BsmBI CGTCTCn'nnnn_ Cuts at: 0 188 744 Size: 188 556 Bsp1286I G_dGCh'C Cuts at: 0 321 590 744 Size: 321 269 154 Fragments arranged by size: 321 269 154 BspHI T'CATG_A Cuts at: 0 546 744 Size: 546 198 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 421 744 Size: 421 323 BstBI TT'CG_AA Cuts at: 0 516 744 Size: 516 228 BstYI r'GATC_y Cuts at: 0 541 610 727 744 Size: 541 69 117 17 Fragments arranged by size: 541 117 69 17 BtgI C'CryG_G Cuts at: 0 150 276 744 Size: 150 126 468 Fragments arranged by size: 468 150 126 DraI TTT'AAA Cuts at: 0 410 744 Size: 410 334 EaeI y'GGCC_r Cuts at: 0 273 744 Size: 273 471 EagI C'GGCC_G Cuts at: 0 273 744 Size: 273 471 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 329 744 Size: 329 415 EcoICRI GAG'CTC Cuts at: 0 319 744 Size: 319 425 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 56 744 Size: 56 688 EcoO109I rG'GnC_Cy Cuts at: 0 186 744 Size: 186 558 HaeII r_GCGC'y Cuts at: 0 122 744 Size: 122 622 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 356 388 744 Size: 356 32 356 Fragments arranged by size: 356 356 32 KpnI G_GTAC'C Cuts at: 0 246 744 Size: 246 498 MslI CAynn'nnrTG Cuts at: 0 55 422 744 Size: 55 367 322 Fragments arranged by size: 367 322 55 MspA1I CmG'CkG Cuts at: 0 152 744 Size: 152 592 NruI TCG'CGA Cuts at: 0 221 744 Size: 221 523 NspI r_CATG'y Cuts at: 0 423 744 Size: 423 321 PflMI CCAn_nnn'nTGG Cuts at: 0 604 744 Size: 604 140 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 88 120 744 Size: 88 32 624 Fragments arranged by size: 624 88 32 PpuMI rG'GwC_Cy Cuts at: 0 186 744 Size: 186 558 PspOMI G'GGCC_C Cuts at: 0 586 744 Size: 586 158 SacI G_AGCT'C Cuts at: 0 321 744 Size: 321 423 SacII CC_GC'GG Cuts at: 0 153 744 Size: 153 591 SmlI C'TyrA_G Cuts at: 0 139 744 Size: 139 605 StyI C'CwwG_G Cuts at: 0 86 682 744 Size: 86 596 62 Fragments arranged by size: 596 86 62 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 31 744 Size: 31 713 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 395 744 Size: 395 349 Enzymes that do cut: AarI Acc65I AflIII AleI ApaI AvaI BanI BanII BbsI BcgI BglI BglII Bme1580I BmgBI BpmI BpuEI BsaAI BsaBI BsaXI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BstBI BstYI BtgI DraI EaeI EagI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I KpnI MslI MspA1I NruI NspI PflMI PpiI PpuMI PspOMI SacI SacII SmlI StyI TstI XcmI Enzymes that do not cut: AatII AccI AclI AcuI AfeI AflII AgeI AhdI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BclI BfrBI BlpI BmrI BmtI BplI Bpu10I BsaI BsaHI BsaWI BseRI BseYI BsgI BsiWI BsmI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII DrdI EarI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI MfeI MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI PacI PasI PciI PfoI PmeI PmlI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI XhoI XmaI XmnI ZraI