Restriction map for
pFN21AE5188
##################################
# NO CUT ENZYMES ( 192 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BcnI
BfrI BglII BlnI BmyI BpuAI
BsaBI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsiYI BslI BsmAI BsmBI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cac8I
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I FauI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MslI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIV NotI
NruI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI Tth111I
TthHB8I Van91I XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 367 GTCTAC GT^[AC][GT]AC
AfaI 3 GTAC GT^AC
AlwI 21 GGATC GGATC(4/5)
BbvI 323 GCAGC GCAGC(8/12)
BclI 66 TGATCA T^GATCA
BfaI 220 CTAG C^TAG
BglI 94 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 177 GCTGAGC GC^T[ACGT]AGC
BpmI 262 CTCCAG CTGGAG(16/14)
Bpu1102I 177 GCTGAGC GC^T[ACGT]AGC
BsaAI 4 TACGTG [CT]AC^GT[GA]
BsmFI 13 GGGAC GGGAC(10/14)
BsrI 18 ACTGG ACTGG(1/-1)
BstF5I 46 CATCC GGATG(2/0)
BstYI 21 GGATCT [GA]^GATC[CT]
CelII 177 GCTGAGC GC^T[ACGT]AGC
Cfr13I 93 GGCCC G^G[ACGT]CC
EarI 223 GAAGAG CTCTTC(1/4)
EcoT22I 43 ATGCAT ATGCA^T
FbaI 66 TGATCA T^GATCA
Fnu4HI 323 GCAGC GC^[ACGT]GC
FokI 46 CATCC GGATG(9/13)
HincII 85 GTTAAC GT[CT]^[GA]AC
HindII 85 GTTAAC GT[CT]^[GA]AC
HindIII 156 AAGCTT A^AGCTT
HinfI 308 GATTC G^A[ACGT]TC
HpaI 85 GTTAAC GTT^AAC
ItaI 323 GCAGC GC^[ACGT]GC
Ksp632I 223 GAAGAG CTCTTC(1/4)
MaeI 220 CTAG C^TAG
MaeII 5 ACGT A^CGT
MaeIII 146 GTCAC ^GT[ACGT]AC
MflI 21 GGATCT [GA]^GATC[CT]
MspA1I 175 CAGCTG C[AC]G^C[GT]G
NsiI 43 ATGCAT ATGCA^T
PvuII 175 CAGCTG CAG^CTG
RsaI 3 GTAC GT^AC
Sau96I 93 GGCCC G^G[ACGT]CC
SfaNI 45 GCATC GCATC(5/9)
TfiI 308 GATTC G^A[AT]TC
TseI 323 GCAGC G^C[AT]GC
Tsp45I 146 GTCAC ^GT[GC]AC
XbaI 219 TCTAGA T^CTAGA
XhoII 21 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcsI 230 AAATTC [GA]^AATT[CT]
350 AAATTT
ApoI 230 AAATTC [GA]^AATT[CT]
350 AAATTT
BssKI 264 CCAGG ^CC[ACGT]GG
319 CCTGG
BstNI 264 CCAGG CC^[AT]GG
319 CCTGG
DdeI 178 CTGAG C^T[ACGT]AG
283 CTAAG
DpnII 22 GATC ^GATC
67 GATC
EcoRII 264 CCAGG ^CC[AT]GG
319 CCTGG
HaeIII 93 GGCC GG^CC
383 GGCC
MboI 22 GATC ^GATC
67 GATC
MboII 223 GAAGA GAAGA(8/7)
330 GAAGA
MvaI 264 CCAGG CC^[AT]GG
319 CCTGG
NdeII 22 GATC ^GATC
67 GATC
NlaIII 42 CATG CATG^
90 CATG
Sau3AI 22 GATC ^GATC
67 GATC
ScrFI 264 CCAGG CC^[ACGT]GG
319 CCTGG
TspRI 17 CACTG CA[GC]TG[ACGT][ACGT]^
275 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 6 enzymes) #
#########################################
AluI MnlI MseI MwoI Tru9I
Tsp509I