Restriction map for pFN21AE5188
 (Circular) (Six-base) MAPSORT of: pFN21AE5188.seq  Check: 4376  from: 1  to: 5092



pFN21AE5188  5092 bp

 With 182 enzymes: SgfI * 

                        January 30, 2014 17:14  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2902     278 
   Size:         53      83     186     936    1366    2468

  Fragments arranged by size: 

                2468    1366     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2373    2435    1937 
   Size:        436      62    4594

  Fragments arranged by size: 

                4594     436      62
 
Acc65I G'GTAC_C

Cuts at:   2413    2413 
   Size:       5092
 
AclI AA'CG_TT

Cuts at:   1563    3219    3592    1563 
   Size:       1656     373    3063

  Fragments arranged by size: 

                3063    1656     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3155    4539    4807    1632 
   Size:       1523    1384     268    1917

  Fragments arranged by size: 

                1917    1523    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4895
 
AflIII A'CryG_T

Cuts at:   1203    3991    4968    1203 
   Size:       2788     977    1327

  Fragments arranged by size: 

                2788    1327     977
 
AgeI A'CCGG_T

Cuts at:   3923    3923 
   Size:       5092
 
AhdI GACnn_n'nnGTC

Cuts at:   3821    3821 
   Size:       5092
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5092
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5092
 
AlwNI CAG_nnn'CTG

Cuts at:   2835    4407    2835 
   Size:       1572    3520
 
ApaLI G'TGCA_C

Cuts at:   3149    4305    3149 
   Size:       1156    3936
 
ApoI r'AATT_y

Cuts at:   1493    2235    2355    2401    2627    2779    4888    1493 
   Size:        742     120      46     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     742     226     152     120      46
 
AseI AT'TA_AT

Cuts at:    160    1032    3646     160 
   Size:        872    2614    1606

  Fragments arranged by size: 

                2614    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5092
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2417    1870 
   Size:         73     474    4545

  Fragments arranged by size: 

                4545     474      73
 
BamHI G'GATC_C

Cuts at:   2422    2789    2422 
   Size:        367    4725
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2413    3868     618 
   Size:        325     217     627     626    1455    1842

  Fragments arranged by size: 

                1842    1455     627     626     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2411    1391 
   Size:       1020    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4121
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3283    3317    1903 
   Size:         34    1346      34    3678

  Fragments arranged by size: 

                3678    1346      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2984    4194    2984 
   Size:       1210    3882
 
BclI T'GATC_A

Cuts at:   1548    2071    1548 
   Size:        523    4569
 
BfrBI ATG'CAT

Cuts at:   2050    2050 
   Size:       5092
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2105    3703     136 
   Size:        107     122      71     631     605     433    1598    1525

  Fragments arranged by size: 

                1598    1525     631     605     433     122     107      71
            
 
BglII A'GATC_T

Cuts at:   5087    5087 
   Size:       5092
 
BlpI GC'TnA_GC

Cuts at:   2183    2509    2183 
   Size:        326    4766
 
Bme1580I G_kGCm'C

Cuts at:   1790    3153    4309    1790 
   Size:       1363    1156    2573

  Fragments arranged by size: 

                2573    1363    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5092
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2823    3781     442 
   Size:        416      53      83     399      24    1406     958    1753

  Fragments arranged by size: 

                1753    1406     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5092
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2252    3752    1239 
   Size:       1013    1500    2579

  Fragments arranged by size: 

                2579    1500    1013
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3217    4082    4380    3217 
   Size:        865     298    3929

  Fragments arranged by size: 

                3929     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3755     882 
   Size:        617    2256    2219

  Fragments arranged by size: 

                2256    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2011    2883     493 
   Size:        711     807     872    2702

  Fragments arranged by size: 

                2702     872     807     711
 
BsaBI GATnn'nnATC

Cuts at:   5086    5086 
   Size:       5092
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2899    3281    3916     275 
   Size:         53      83     186     936    1366     382     635    1451

  Fragments arranged by size: 

                1451    1366     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3525    3923    4197    4344    4755    3525 
   Size:        398     274     147     411    3862

  Fragments arranged by size: 

                3862     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4867

  Fragments arranged by size: 

                4867     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5092
 
BseYI C'CCAG_C

Cuts at:   4295    4791    4295 
   Size:        496    4596
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4519
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2542    3303    4331    1540 
   Size:        275     186     541     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     541     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3153    3238    4309    3153 
   Size:         85    1071    3936

  Fragments arranged by size: 

                3936    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2603    2696    2603 
   Size:         93    4999
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2411    3153    3238    4309    1391 
   Size:        399     621     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     621     399      85
 
BspEI T'CCGG_A

Cuts at:   4755    4755 
   Size:       5092
 
BspHI T'CATG_A

Cuts at:   2981    4711    2981 
   Size:       1730    3362
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2447     844 
   Size:       1603    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2979    1394 
   Size:        429    1156    3507

  Fragments arranged by size: 

                3507    1156     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3587    3761      65 
   Size:       1183    2339     174    1396

  Fragments arranged by size: 

                2339    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3736    3923    1700 
   Size:        110    1926     187    2869

  Fragments arranged by size: 

                2869    1926     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5092
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5092
 
BssSI C'ACGA_G

Cuts at:   3152    4164    3152 
   Size:       1012    4080
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5092
 
BstBI TT'CG_AA

Cuts at:   3896    3896 
   Size:       5092
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5092
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2011    2011 
   Size:       5092
 
BstYI r'GATC_y

Cuts at:   1972    2026    2422    2789    3174    3191    4643    4729    4741 
   Size:         54     396     367     385      17    1452      86      12

Cuts at:   4741    5087    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     396     385     367     346      86      54
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    4540
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4353

  Fragments arranged by size: 

                4353     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2608    3402    3422    2608 
   Size:        794      20    4278

  Fragments arranged by size: 

                4278     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5092
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5057
 
DraI TTT'AAA

Cuts at:   2396    2396 
   Size:       5092
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4099     809 
   Size:        507    2783    1802

  Fragments arranged by size: 

                2783    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2539    3428    5057 
   Size:         54    1425      50     275      18     709     889    1629

Cuts at:   5057       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     709     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2539    1537 
   Size:        275     727    4090

  Fragments arranged by size: 

                4090     727     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2223    3022    1353 
   Size:        870     799    3423

  Fragments arranged by size: 

                3423     870     799
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3679    4053    4199     387 
   Size:       1064     138    2090     374     146    1280

  Fragments arranged by size: 

                2090    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2252    3155    3752    4539    4807    1239 
   Size:        393     620     903     597     787     268    1524

  Fragments arranged by size: 

                1524     903     787     620     597     393     268
 
EcoRI G'AATT_C

Cuts at:   2401    2779    2401 
   Size:        378    4714
 
FspI TGC'GCA

Cuts at:   3598    3598 
   Size:       5092
 
HaeII r_GCGC'y

Cuts at:   1678    4239    1678 
   Size:       2561    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2011    2043    3733    3765    3807 
   Size:         32     135      32     439      32    1690      32      42

Cuts at:   3807    3839    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1690     439     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2092    2436    2682     669 
   Size:        988     281     154     344     246    3079

  Fragments arranged by size: 

                3079     988     344     281     246     154
 
HindIII A'AGCT_T

Cuts at:    748    2161     748 
   Size:       1413    3679
 
HpaI GTT'AAC

Cuts at:   2092    2682    2092 
   Size:        590    4502
 
KpnI G_GTAC'C

Cuts at:   2417    2417 
   Size:       5092
 
MfeI C'AATT_G

Cuts at:   2691    2691 
   Size:       5092
 
MluI A'CGCG_T

Cuts at:   4968    4968 
   Size:       5092
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4206    4390    1305 
   Size:       2901     184    2007

  Fragments arranged by size: 

                2901    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3270

  Fragments arranged by size: 

                3270    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2009    3050    3409    3568     518 
   Size:        648     843    1041     359     159    2042

  Fragments arranged by size: 

                2042    1041     843     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2182    2508    3185    4333    4578    1606 
   Size:        576     326     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     576     326     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4540
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5092
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5092
 
NotI GC'GGCC_GC

Cuts at:   2539    2539 
   Size:       5092
 
NsiI A_TGCA'T

Cuts at:   2052    2052 
   Size:       5092
 
NspI r_CATG'y

Cuts at:   1132    1229    2450    3995    1132 
   Size:         97    1221    1545    2229

  Fragments arranged by size: 

                2229    1545    1221      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5092
 
PciI A'CATG_T

Cuts at:   3991    3991 
   Size:       5092
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5092
 
PfoI T'CCnGG_A

Cuts at:   2268    2268 
   Size:       5092
 
PmeI GTTT'AAAC

Cuts at:   2396    2396 
   Size:       5092
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3161    3193    4674    4706    3161 
   Size:         32    1481      32    3547

  Fragments arranged by size: 

                3547    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5092
 
PsiI TTA'TAA

Cuts at:   2662    2662 
   Size:       5092
 
PstI C_TGCA'G

Cuts at:    830    2444     830 
   Size:       1614    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5092
 
PvuII CAG'CTG

Cuts at:   2182    2182 
   Size:       5092
 
SalI G'TCGA_C

Cuts at:   1936    2434    1936 
   Size:        498    4594
 
SbfI CC_TGCA'GG

Cuts at:   2444    2444 
   Size:       5092
 
ScaI AGT'ACT

Cuts at:   3340    5052    3340 
   Size:       1712    3380
 
SfcI C'TryA_G

Cuts at:    826    1045    2440    3575    4256    4447     826 
   Size:        219    1395    1135     681     191    1471

  Fragments arranged by size: 

                1471    1395    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5092
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5092
 
SmaI CCC'GGG

Cuts at:   1872    2419    1872 
   Size:        547    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3196    4097    4359     820 
   Size:        197     926    1253     901     262    1553

  Fragments arranged by size: 

                1553    1253     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2883     493 
   Size:       2390    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5092
 
SphI G_CATG'C

Cuts at:   1132    2450    1132 
   Size:       1318    3774
 
SspI AAT'ATT

Cuts at:      5      52    3016       5 
   Size:         47    2964    2081

  Fragments arranged by size: 

                2964    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5092
 
StyI C'CwwG_G

Cuts at:    513    1065    2531     513 
   Size:        552    1466    3074

  Fragments arranged by size: 

                3074    1466     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3119    3136    3289    3474    3919     325 
   Size:       1255    1539      17     153     185     445    1498

  Fragments arranged by size: 

                1539    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3338    5050      96 
   Size:        274      80      33      51    1443    1361    1712     138

  Fragments arranged by size: 

                1712    1443    1361     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5052    5084    5052 
   Size:         32    5060
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5092
 
XbaI T'CTAG_A

Cuts at:   2224    2428    2224 
   Size:        204    4888
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5092
 
XmaI C'CCGG_G

Cuts at:   1870    2417    1870 
   Size:        547    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3221    3221 
   Size:       5092
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2900     276 
   Size:         53      83     186     936    1366    2468

  Fragments arranged by size: 

                2468    1366     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NheI     NotI     NsiI     NspI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PshAI     PsiI     PstI     PvuI    PvuII
     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI     BplI   Bpu10I    BsiWI    BsmBI  BstZ17I   Bsu36I   DraIII
  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI     KasI
     NaeI     NarI   NgoMIV     NruI     PacI     PmlI    PpuMI   PspOMI
    PspXI     PsrI    RsrII     SacI    SacII    SanDI     SapI    SexAI
     SfiI     SfoI     SrfI     SwaI     XcmI
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