(Circular) (Six-base) MAPSORT of: pFN21AE5188.seq Check: 4376 from: 1 to: 5092 pFN21AE5188 5092 bp With 182 enzymes: SgfI * January 30, 2014 17:14 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2902 278 Size: 53 83 186 936 1366 2468 Fragments arranged by size: 2468 1366 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2373 2435 1937 Size: 436 62 4594 Fragments arranged by size: 4594 436 62 Acc65I G'GTAC_C Cuts at: 2413 2413 Size: 5092 AclI AA'CG_TT Cuts at: 1563 3219 3592 1563 Size: 1656 373 3063 Fragments arranged by size: 3063 1656 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3155 4539 4807 1632 Size: 1523 1384 268 1917 Fragments arranged by size: 1917 1523 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4895 AflIII A'CryG_T Cuts at: 1203 3991 4968 1203 Size: 2788 977 1327 Fragments arranged by size: 2788 1327 977 AgeI A'CCGG_T Cuts at: 3923 3923 Size: 5092 AhdI GACnn_n'nnGTC Cuts at: 3821 3821 Size: 5092 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5092 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5092 AlwNI CAG_nnn'CTG Cuts at: 2835 4407 2835 Size: 1572 3520 ApaLI G'TGCA_C Cuts at: 3149 4305 3149 Size: 1156 3936 ApoI r'AATT_y Cuts at: 1493 2235 2355 2401 2627 2779 4888 1493 Size: 742 120 46 226 152 2109 1697 Fragments arranged by size: 2109 1697 742 226 152 120 46 AseI AT'TA_AT Cuts at: 160 1032 3646 160 Size: 872 2614 1606 Fragments arranged by size: 2614 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5092 AvaI C'yCGr_G Cuts at: 1870 1943 2417 1870 Size: 73 474 4545 Fragments arranged by size: 4545 474 73 BamHI G'GATC_C Cuts at: 2422 2789 2422 Size: 367 4725 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2413 3868 618 Size: 325 217 627 626 1455 1842 Fragments arranged by size: 1842 1455 627 626 325 217 BanII G_rGCy'C Cuts at: 1391 2411 1391 Size: 1020 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4121 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3283 3317 1903 Size: 34 1346 34 3678 Fragments arranged by size: 3678 1346 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2984 4194 2984 Size: 1210 3882 BclI T'GATC_A Cuts at: 1548 2071 1548 Size: 523 4569 BfrBI ATG'CAT Cuts at: 2050 2050 Size: 5092 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2105 3703 136 Size: 107 122 71 631 605 433 1598 1525 Fragments arranged by size: 1598 1525 631 605 433 122 107 71 BglII A'GATC_T Cuts at: 5087 5087 Size: 5092 BlpI GC'TnA_GC Cuts at: 2183 2509 2183 Size: 326 4766 Bme1580I G_kGCm'C Cuts at: 1790 3153 4309 1790 Size: 1363 1156 2573 Fragments arranged by size: 2573 1363 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5092 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2823 3781 442 Size: 416 53 83 399 24 1406 958 1753 Fragments arranged by size: 1753 1406 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5092 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2252 3752 1239 Size: 1013 1500 2579 Fragments arranged by size: 2579 1500 1013 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3217 4082 4380 3217 Size: 865 298 3929 Fragments arranged by size: 3929 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3755 882 Size: 617 2256 2219 Fragments arranged by size: 2256 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2011 2883 493 Size: 711 807 872 2702 Fragments arranged by size: 2702 872 807 711 BsaBI GATnn'nnATC Cuts at: 5086 5086 Size: 5092 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2899 3281 3916 275 Size: 53 83 186 936 1366 382 635 1451 Fragments arranged by size: 1451 1366 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3525 3923 4197 4344 4755 3525 Size: 398 274 147 411 3862 Fragments arranged by size: 3862 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4867 Fragments arranged by size: 4867 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5092 BseYI C'CCAG_C Cuts at: 4295 4791 4295 Size: 496 4596 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4519 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2542 3303 4331 1540 Size: 275 186 541 761 1028 2301 Fragments arranged by size: 2301 1028 761 541 275 186 BsiHKAI G_wGCw'C Cuts at: 3153 3238 4309 3153 Size: 85 1071 3936 Fragments arranged by size: 3936 1071 85 BsmI GAATG_Cn' Cuts at: 2603 2696 2603 Size: 93 4999 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2411 3153 3238 4309 1391 Size: 399 621 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 621 399 85 BspEI T'CCGG_A Cuts at: 4755 4755 Size: 5092 BspHI T'CATG_A Cuts at: 2981 4711 2981 Size: 1730 3362 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2447 844 Size: 1603 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2979 1394 Size: 429 1156 3507 Fragments arranged by size: 3507 1156 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3587 3761 65 Size: 1183 2339 174 1396 Fragments arranged by size: 2339 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3736 3923 1700 Size: 110 1926 187 2869 Fragments arranged by size: 2869 1926 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5092 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5092 BssSI C'ACGA_G Cuts at: 3152 4164 3152 Size: 1012 4080 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5092 BstBI TT'CG_AA Cuts at: 3896 3896 Size: 5092 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5092 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5092 BstYI r'GATC_y Cuts at: 1972 2026 2422 2789 3174 3191 4643 4729 4741 Size: 54 396 367 385 17 1452 86 12 Cuts at: 4741 5087 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 396 385 367 346 86 54 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4540 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4353 Fragments arranged by size: 4353 683 56 BtsI GCAGTG_nn' Cuts at: 2608 3402 3422 2608 Size: 794 20 4278 Fragments arranged by size: 4278 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5092 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5057 DraI TTT'AAA Cuts at: 2396 2396 Size: 5092 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4099 809 Size: 507 2783 1802 Fragments arranged by size: 2783 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2539 3428 5057 Size: 54 1425 50 275 18 709 889 1629 Cuts at: 5057 8 Size: 43 Fragments arranged by size: 1629 1425 889 709 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2539 1537 Size: 275 727 4090 Fragments arranged by size: 4090 727 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2223 3022 1353 Size: 870 799 3423 Fragments arranged by size: 3423 870 799 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3679 4053 4199 387 Size: 1064 138 2090 374 146 1280 Fragments arranged by size: 2090 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2252 3155 3752 4539 4807 1239 Size: 393 620 903 597 787 268 1524 Fragments arranged by size: 1524 903 787 620 597 393 268 EcoRI G'AATT_C Cuts at: 2401 2779 2401 Size: 378 4714 FspI TGC'GCA Cuts at: 3598 3598 Size: 5092 HaeII r_GCGC'y Cuts at: 1678 4239 1678 Size: 2561 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2011 2043 3733 3765 3807 Size: 32 135 32 439 32 1690 32 42 Cuts at: 3807 3839 1373 Size: 32 2626 Fragments arranged by size: 2626 1690 439 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2092 2436 2682 669 Size: 988 281 154 344 246 3079 Fragments arranged by size: 3079 988 344 281 246 154 HindIII A'AGCT_T Cuts at: 748 2161 748 Size: 1413 3679 HpaI GTT'AAC Cuts at: 2092 2682 2092 Size: 590 4502 KpnI G_GTAC'C Cuts at: 2417 2417 Size: 5092 MfeI C'AATT_G Cuts at: 2691 2691 Size: 5092 MluI A'CGCG_T Cuts at: 4968 4968 Size: 5092 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4206 4390 1305 Size: 2901 184 2007 Fragments arranged by size: 2901 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3270 Fragments arranged by size: 3270 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3050 3409 3568 518 Size: 648 843 1041 359 159 2042 Fragments arranged by size: 2042 1041 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2182 2508 3185 4333 4578 1606 Size: 576 326 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 576 326 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4540 NdeI CA'TA_TG Cuts at: 387 387 Size: 5092 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5092 NotI GC'GGCC_GC Cuts at: 2539 2539 Size: 5092 NsiI A_TGCA'T Cuts at: 2052 2052 Size: 5092 NspI r_CATG'y Cuts at: 1132 1229 2450 3995 1132 Size: 97 1221 1545 2229 Fragments arranged by size: 2229 1545 1221 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5092 PciI A'CATG_T Cuts at: 3991 3991 Size: 5092 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5092 PfoI T'CCnGG_A Cuts at: 2268 2268 Size: 5092 PmeI GTTT'AAAC Cuts at: 2396 2396 Size: 5092 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3161 3193 4674 4706 3161 Size: 32 1481 32 3547 Fragments arranged by size: 3547 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5092 PsiI TTA'TAA Cuts at: 2662 2662 Size: 5092 PstI C_TGCA'G Cuts at: 830 2444 830 Size: 1614 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5092 PvuII CAG'CTG Cuts at: 2182 2182 Size: 5092 SalI G'TCGA_C Cuts at: 1936 2434 1936 Size: 498 4594 SbfI CC_TGCA'GG Cuts at: 2444 2444 Size: 5092 ScaI AGT'ACT Cuts at: 3340 5052 3340 Size: 1712 3380 SfcI C'TryA_G Cuts at: 826 1045 2440 3575 4256 4447 826 Size: 219 1395 1135 681 191 1471 Fragments arranged by size: 1471 1395 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5092 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5092 SmaI CCC'GGG Cuts at: 1872 2419 1872 Size: 547 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3196 4097 4359 820 Size: 197 926 1253 901 262 1553 Fragments arranged by size: 1553 1253 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2883 493 Size: 2390 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5092 SphI G_CATG'C Cuts at: 1132 2450 1132 Size: 1318 3774 SspI AAT'ATT Cuts at: 5 52 3016 5 Size: 47 2964 2081 Fragments arranged by size: 2964 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5092 StyI C'CwwG_G Cuts at: 513 1065 2531 513 Size: 552 1466 3074 Fragments arranged by size: 3074 1466 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3119 3136 3289 3474 3919 325 Size: 1255 1539 17 153 185 445 1498 Fragments arranged by size: 1539 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3338 5050 96 Size: 274 80 33 51 1443 1361 1712 138 Fragments arranged by size: 1712 1443 1361 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5052 5084 5052 Size: 32 5060 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5092 XbaI T'CTAG_A Cuts at: 2224 2428 2224 Size: 204 4888 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5092 XmaI C'CCGG_G Cuts at: 1870 2417 1870 Size: 547 4545 XmnI GAAnn'nnTTC Cuts at: 3221 3221 Size: 5092 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2900 276 Size: 53 83 186 936 1366 2468 Fragments arranged by size: 2468 1366 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI