################################## # NO CUT ENZYMES ( 186 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeI MaeII MfeI MflI MluI MluNI MroI MscI MseI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AlwI 323 GGATC GGATC(4/5) AlwNI 147 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 68 GGTCC G^G[AT]CC BpmI 274 CTGGAG CTGGAG(16/14) BsaBI 133 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 166 GAGACC GGTCTC(1/5) BsgI 77 CTGCAC GTGCAG(16/14) BsiYI 331 CCTCACCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 331 CCTCACCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 166 GAGAC GTCTC(1/5) BsmFI 69 GTCCC GGGAC(10/14) BsmI 385 GAATGC GAATGC(1/-1) BsrDI 260 GCAATG GCAATG(2/0) Cac8I 341 GCCAGC GC[ACGT]^[ACGT]GC CfoI 144 GCGC GCG^C DdeI 290 CTGAG C^T[ACGT]AG DraII 269 AGGGCCT [GA]G^G[ACGT]CC[CT] DraIII 112 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 83 CCATGG C^C[GA][CT]GG EcoNI 331 CCTCACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 269 AGGGCCT [GA]G^G[ACGT]CC[CT] FauI 248 GCGGG CCCGC(4/6) HhaI 144 GCGC GCG^C HinP1I 144 GCGC G^CGC MaeIII 425 GTGAC ^GT[ACGT]AC MamI 133 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 303 CATCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 83 CCATGG C^CATGG PleI 295 GAGTC GAGTC(4/5) SfcI 367 CTACAG C^T[GA][CT]AG TfiI 105 GAATC G^A[AT]TC Tsp45I 425 GTGAC ^GT[GC]AC TspRI 288 CACTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AfaI 192 GTAC GT^AC 210 GTAC AluI 148 AGCT AG^CT 361 AGCT BmyI 232 GTGCCC G[AGT]GC[ACT]^C 283 GTGCCC BseRI 95 GAGGAG GAGGAG(10/8) 292 GAGGAG Bsp1286I 232 GTGCCC G[AGT]GC[ACT]^C 283 GTGCCC BstAPI 146 GCAGCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 312 GCAGATTATGC Cfr13I 68 GGTCC G^G[ACGT]CC 270 GGGCC DpnII 204 GATC ^GATC 324 GATC EcoT14I 83 CCATGG C^C[AT][AT]GG 336 CCAAGG HaeIII 271 GGCC GG^CC 340 GGCC HinfI 105 GAATC G^A[ACGT]TC 295 GAGTC MboI 204 GATC ^GATC 324 GATC MboII 182 TCTTC GAAGA(8/7) 215 TCTTC NdeII 204 GATC ^GATC 324 GATC NlaIV 29 GGAGCC GG[ACGT]^[ACGT]CC 68 GGTCCC RsaI 192 GTAC GT^AC 210 GTAC Sau3AI 204 GATC ^GATC 324 GATC Sau96I 68 GGTCC G^G[ACGT]CC 270 GGGCC SfaNI 240 GATGC GCATC(5/9) 245 GATGC StyI 83 CCATGG C^C[AT][AT]GG 336 CCAAGG TaqI 164 TCGA T^CGA 206 TCGA TthHB8I 164 TCGA T^CGA 206 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 17 enzymes) # ######################################### AciI BbvI BsaJI BssKI BstF5I BstNI EcoRII Fnu4HI FokI HphI ItaI MnlI MvaI MwoI NlaIII ScrFI TseI