################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspHI AspI AviII AvrII BanI BanII BbeI BbrPI BbsI BbvCI BcgI BcnI BglI BlnI BmyI BpuAI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspDI BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DrdI DsaI EagI EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FseI FspI HaeII HapII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MluI MroI MspA1I MspI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AcyI 127 GACGCC G[GA]^CG[CT]C AflII 432 CTTAAG C^TTAAG AflIII 363 ACATGT A^C[GA][CT]GT AhdI 1832 GACGAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 120 ATTAAT AT^TAAT AsnI 120 ATTAAT AT^TAAT AspEI 1832 GACGAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 838 TGGCCA TGG^CCA BciVI 774 GTATCC GTATCC(6/5) BclI 99 TGATCA T^GATCA BfrI 432 CTTAAG C^TTAAG BlpI 1687 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1687 GCTGAGC GC^T[ACGT]AGC BsaHI 127 GACGCC G[GA]^CG[CT]C BseRI 2066 GAGGAG GAGGAG(10/8) BsmBI 551 GAGACG CGTCTC(1/5) Bsp1407I 1397 TGTACA T^GTACA BspLU11I 363 ACATGT A^CATGT BsrDI 745 GCAATG GCAATG(2/0) BsrGI 1397 TGTACA T^GTACA BstAPI 630 GCATTTAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 1687 GCTGAGC GC^T[ACGT]AGC DraI 1018 TTTAAA TTT^AAA DraII 2015 AGGACCC [GA]G^G[ACGT]CC[CT] EaeI 838 TGGCCA [CT]^GGCC[GA] Eam1105I 1832 GACGAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1657 CCTTCTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 2015 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRI 966 GAATTC G^AATTC FauI 2021 CCCGC CCCGC(4/6) FbaI 99 TGATCA T^GATCA HaeIII 839 GGCC GG^CC Hin1I 127 GACGCC G[GA]^CG[CT]C MfeI 769 CAATTG C^AATTG MluNI 838 TGGCCA TGG^CCA MscI 838 TGGCCA TGG^CCA MunI 769 CAATTG C^AATTG NdeI 1367 CATATG CA^TATG PpuMI 2015 AGGACCC [GA]G^G[AT]CC[CT] PshBI 120 ATTAAT AT^TAAT PstI 1490 CTGCAG CTGCA^G SspBI 1397 TGTACA T^GTACA Tsp45I 1036 GTCAC ^GT[GC]AC XbaI 1158 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### Asp700I 1045 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1495 GAAATTCTTC AvaI 902 CTCGGG C^[CT]CG[GA]G 1545 CTCGGG AvaII 1070 GGTCC G^G[AT]CC 2016 GGACC BamHI 298 GGATCC G^GATCC 897 GGATCC BglII 373 AGATCT A^GATCT 543 AGATCT BsaAI 972 TACGTA [CT]AC^GT[GA] 1294 TACGTA BsaBI 919 GATAGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1531 GATGTCAATC BsmFI 1878 GGGAC GGGAC(10/14) 1957 GGGAC BsoBI 902 CTCGGG C^[CT]CG[GA]G 1545 CTCGGG CfoI 1136 GCGC GCG^C 1763 GCGC Cfr13I 1070 GGTCC G^G[ACGT]CC 2016 GGACC HhaI 1136 GCGC GCG^C 1763 GCGC HinP1I 1136 GCGC G^CGC 1763 GCGC MamI 919 GATAGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1531 GATGTCAATC NspI 363 ACATGT [GA]CATG^[CT] 713 ACATGC PflMI 174 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1753 CCAGTGATTGG Sau96I 1070 GGTCC G^G[ACGT]CC 2016 GGACC SmlI 432 CTTAAG C^T[CT][GA]AG 1081 CTTGAG SnaBI 972 TACGTA TAC^GTA 1294 TACGTA Van91I 174 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1753 CCAGTGATTGG XmnI 1045 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1495 GAAATTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI BbvI BfaI BpmI BsaJI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI BstYI Cac8I DdeI DpnII DraIII EcoRII EcoT14I Fnu4HI FokI HgaI HincII HindII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII