(Circular) (Six-base) MAPSORT of: pFN21AE5754.seq Check: 46 from: 1 to: 6913 pFN21AE5754 6913 bp With 182 enzymes: SgfI * December 8, 2014 13:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4723 278 Size: 53 83 186 936 3187 2468 Fragments arranged by size: 3187 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4256 1937 Size: 2319 4594 Acc65I G'GTAC_C Cuts at: 4234 4234 Size: 6913 AclI AA'CG_TT Cuts at: 1563 5040 5413 1563 Size: 3477 373 3063 Fragments arranged by size: 3477 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2882 4056 4976 6360 6628 1632 Size: 1250 1174 920 1384 268 1917 Fragments arranged by size: 1917 1384 1250 1174 920 268 AflII C'TTAA_G Cuts at: 820 1017 2437 820 Size: 197 1420 5296 Fragments arranged by size: 5296 1420 197 AflIII A'CryG_T Cuts at: 1203 2368 5812 6789 1203 Size: 1165 3444 977 1327 Fragments arranged by size: 3444 1327 1165 977 AgeI A'CCGG_T Cuts at: 5744 5744 Size: 6913 AhdI GACnn_n'nnGTC Cuts at: 3842 5642 3842 Size: 1800 5113 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6913 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2785 1184 Size: 1601 5312 AlwNI CAG_nnn'CTG Cuts at: 4656 6228 4656 Size: 1572 5341 ApaLI G'TGCA_C Cuts at: 4970 6126 4970 Size: 1156 5757 ApoI r'AATT_y Cuts at: 1493 2289 2474 2480 2628 2887 2971 3020 3051 Size: 796 185 6 148 259 84 49 31 Cuts at: 3051 3097 3227 3501 4186 4222 4448 4600 6709 Size: 46 130 274 685 36 226 152 2109 Cuts at: 6709 1493 Size: 1697 Fragments arranged by size: 2109 1697 796 685 274 259 226 185 152 148 130 84 49 46 36 31 6 AseI AT'TA_AT Cuts at: 160 1032 2126 5467 160 Size: 872 1094 3341 1606 Fragments arranged by size: 3341 1606 1094 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6913 AvaI C'yCGr_G Cuts at: 1870 1943 2907 3550 4238 1870 Size: 73 964 643 688 4545 Fragments arranged by size: 4545 964 688 643 73 BamHI G'GATC_C Cuts at: 2303 2902 4243 4610 2303 Size: 599 1341 367 4606 Fragments arranged by size: 4606 1341 599 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4234 5689 618 Size: 325 217 627 2447 1455 1842 Fragments arranged by size: 2447 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4232 1391 Size: 2841 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5942 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5104 5138 1903 Size: 34 3167 34 3678 Fragments arranged by size: 3678 3167 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2790 4805 6015 2790 Size: 2015 1210 3688 Fragments arranged by size: 3688 2015 1210 BclI T'GATC_A Cuts at: 1548 2104 1548 Size: 556 6357 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5524 136 Size: 107 122 71 631 605 3852 1525 Fragments arranged by size: 3852 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2378 2548 6908 2378 Size: 170 4360 2383 Fragments arranged by size: 4360 2383 170 BlpI GC'TnA_GC Cuts at: 3693 4330 3693 Size: 637 6276 Bme1580I G_kGCm'C Cuts at: 1790 4974 6130 1790 Size: 3184 1156 2573 Fragments arranged by size: 3184 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2702 1318 Size: 1384 5529 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3250 4119 4644 Size: 416 53 83 399 24 1833 869 525 Cuts at: 4644 5602 442 Size: 958 1753 Fragments arranged by size: 1833 1753 958 869 525 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6913 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3589 3624 4137 5573 1239 Size: 2350 35 513 1436 2579 Fragments arranged by size: 2579 2350 1436 513 35 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3107 5038 5903 6201 3107 Size: 1931 865 298 3819 Fragments arranged by size: 3819 1931 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5576 882 Size: 617 4077 2219 Fragments arranged by size: 4077 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2979 3301 4704 493 Size: 711 1775 322 1403 2702 Fragments arranged by size: 2702 1775 1403 711 322 BsaBI GATnn'nnATC Cuts at: 2928 3540 6907 2928 Size: 612 3367 2934 Fragments arranged by size: 3367 2934 612 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2133 4720 5102 5737 Size: 53 83 186 936 600 2587 382 635 Cuts at: 5737 275 Size: 1451 Fragments arranged by size: 2587 1451 936 635 600 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5346 5744 6018 6165 6576 5346 Size: 398 274 147 411 5683 Fragments arranged by size: 5683 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6688 Fragments arranged by size: 6688 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4086 1186 Size: 2900 4013 BseYI C'CCAG_C Cuts at: 6116 6612 6116 Size: 496 6417 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6340 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4363 5124 6152 1540 Size: 275 186 2362 761 1028 2301 Fragments arranged by size: 2362 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4974 5059 6130 4974 Size: 85 1071 5757 Fragments arranged by size: 5757 1071 85 BsmI GAATG_Cn' Cuts at: 4424 4517 4424 Size: 93 6820 BsmBI CGTCTCn'nnnn_ Cuts at: 2550 2550 Size: 6913 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4232 4974 5059 6130 1391 Size: 399 2442 742 85 1071 2174 Fragments arranged by size: 2442 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6576 6576 Size: 6913 BspHI T'CATG_A Cuts at: 4802 6532 4802 Size: 1730 5183 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4268 844 Size: 3424 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4800 1394 Size: 429 2977 3507 Fragments arranged by size: 3507 2977 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2757 5408 5582 65 Size: 1183 1509 2651 174 1396 Fragments arranged by size: 2651 1509 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5557 5744 1700 Size: 110 3747 187 2869 Fragments arranged by size: 3747 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3402 96 Size: 3306 3607 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6913 BssSI C'ACGA_G Cuts at: 4973 5985 4973 Size: 1012 5901 BstAPI GCAn_nnn'nTGC Cuts at: 794 2641 794 Size: 1847 5066 BstBI TT'CG_AA Cuts at: 5717 5717 Size: 6913 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6913 BstYI r'GATC_y Cuts at: 1972 2303 2378 2548 2902 3938 4243 4610 4995 Size: 331 75 170 354 1036 305 367 385 Cuts at: 4995 5012 6464 6550 6562 6908 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1036 385 367 354 346 331 305 170 86 75 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6361 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6174 Fragments arranged by size: 6174 683 56 BtsI GCAGTG_nn' Cuts at: 4029 4429 5223 5243 4029 Size: 400 794 20 5699 Fragments arranged by size: 5699 794 400 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6913 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2352 2387 529 Size: 35 1788 35 5055 Fragments arranged by size: 5055 1788 35 35 DraI TTT'AAA Cuts at: 3025 4217 3025 Size: 1192 5721 DraIII CAC_nnn'GTG Cuts at: 2354 3728 3813 2354 Size: 1374 85 5454 Fragments arranged by size: 5454 1374 85 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5920 809 Size: 507 4604 1802 Fragments arranged by size: 4604 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2843 4360 5249 Size: 54 1425 50 275 18 1013 1517 889 Cuts at: 5249 6878 8 Size: 1629 43 Fragments arranged by size: 1629 1517 1425 1013 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4360 1537 Size: 275 2548 4090 Fragments arranged by size: 4090 2548 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4843 1353 Size: 3490 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3570 5500 5874 6020 387 Size: 1064 138 1981 1930 374 146 1280 Fragments arranged by size: 1981 1930 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2882 3589 3624 4056 4137 4976 5573 Size: 393 1250 707 35 432 81 839 597 Cuts at: 5573 6360 6628 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1250 839 787 707 597 432 393 268 81 35 EcoNI CCTnn'n_nnAGG Cuts at: 3666 3666 Size: 6913 EcoO109I rG'GnC_Cy Cuts at: 4021 4021 Size: 6913 EcoRI G'AATT_C Cuts at: 2971 4222 4600 2971 Size: 1251 378 5284 Fragments arranged by size: 5284 1251 378 FspI TGC'GCA Cuts at: 5419 5419 Size: 6913 HaeII r_GCGC'y Cuts at: 1678 6060 1678 Size: 4382 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3368 3400 3828 3860 4133 Size: 32 135 32 1796 32 428 32 273 Cuts at: 4133 4165 4192 4224 5554 5586 5628 5660 1373 Size: 32 27 32 1330 32 42 32 2626 Fragments arranged by size: 2626 1796 1330 428 273 135 42 32 32 32 32 32 32 32 32 27 HincII GTy'rAC Cuts at: 669 1657 1938 2048 3358 3778 4257 4503 669 Size: 988 281 110 1310 420 479 246 3079 Fragments arranged by size: 3079 1310 988 479 420 281 246 110 HindIII A'AGCT_T Cuts at: 748 748 Size: 6913 HpaI GTT'AAC Cuts at: 4503 4503 Size: 6913 KpnI G_GTAC'C Cuts at: 4238 4238 Size: 6913 MfeI C'AATT_G Cuts at: 2774 4512 2774 Size: 1738 5175 MluI A'CGCG_T Cuts at: 6789 6789 Size: 6913 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2739 3154 3187 4115 6027 6211 1305 Size: 1434 415 33 928 1912 184 2007 Fragments arranged by size: 2007 1912 1434 928 415 184 33 MscI TGG'CCA Cuts at: 10 64 1489 1832 2845 10 Size: 54 1425 343 1013 4078 Fragments arranged by size: 4078 1425 1013 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2421 3758 3897 4871 5230 5389 518 Size: 648 1255 1337 139 974 359 159 2042 Fragments arranged by size: 2042 1337 1255 974 648 359 159 139 MspA1I CmG'CkG Cuts at: 1606 4329 5006 6154 6399 1606 Size: 2723 677 1148 245 2120 Fragments arranged by size: 2723 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6361 NdeI CA'TA_TG Cuts at: 387 3373 387 Size: 2986 3927 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6913 NotI GC'GGCC_GC Cuts at: 4360 4360 Size: 6913 NspI r_CATG'y Cuts at: 1132 1229 2372 2722 4271 5816 1132 Size: 97 1143 350 1549 1545 2229 Fragments arranged by size: 2229 1549 1545 1143 350 97 PasI CC'CwG_GG Cuts at: 1348 3266 3880 1348 Size: 1918 614 4381 Fragments arranged by size: 4381 1918 614 PciI A'CATG_T Cuts at: 2368 5812 2368 Size: 3444 3469 PflMI CCAn_nnn'nTGG Cuts at: 1108 2185 3764 1108 Size: 1077 1579 4257 Fragments arranged by size: 4257 1579 1077 PmeI GTTT'AAAC Cuts at: 4217 4217 Size: 6913 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4982 5014 6495 6527 4982 Size: 32 1481 32 5368 Fragments arranged by size: 5368 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4021 4021 Size: 6913 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6913 PsiI TTA'TAA Cuts at: 2471 4483 2471 Size: 2012 4901 PstI C_TGCA'G Cuts at: 830 3499 4265 830 Size: 2669 766 3478 Fragments arranged by size: 3478 2669 766 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6913 SalI G'TCGA_C Cuts at: 1936 4255 1936 Size: 2319 4594 SbfI CC_TGCA'GG Cuts at: 4265 4265 Size: 6913 ScaI AGT'ACT Cuts at: 5161 6873 5161 Size: 1712 5201 SfcI C'TryA_G Cuts at: 826 1045 2308 2445 3495 3666 3783 4261 5396 Size: 219 1263 137 1050 171 117 478 1135 Cuts at: 5396 6077 6268 826 Size: 681 191 1471 Fragments arranged by size: 1471 1263 1135 1050 681 478 219 191 171 137 117 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6913 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6913 SmaI CCC'GGG Cuts at: 1872 4240 1872 Size: 2368 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2437 3086 5017 5918 6180 820 Size: 197 926 494 649 1931 901 262 1553 Fragments arranged by size: 1931 1553 926 901 649 494 262 197 SnaBI TAC'GTA Cuts at: 493 2979 3301 4704 493 Size: 2486 322 1403 2702 Fragments arranged by size: 2702 2486 1403 322 SpeI A'CTAG_T Cuts at: 152 152 Size: 6913 SphI G_CATG'C Cuts at: 1132 4271 1132 Size: 3139 3774 SspI AAT'ATT Cuts at: 5 52 4837 5 Size: 47 4785 2081 Fragments arranged by size: 4785 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6913 StyI C'CwwG_G Cuts at: 513 1065 2731 3316 3742 4352 513 Size: 552 1666 585 426 610 3074 Fragments arranged by size: 3074 1666 610 585 552 426 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4940 4957 5110 5295 5740 325 Size: 1255 3360 17 153 185 445 1498 Fragments arranged by size: 3360 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2320 3199 3402 Size: 274 80 33 51 1443 343 879 203 Cuts at: 3402 5159 6871 96 Size: 1757 1712 138 Fragments arranged by size: 1757 1712 1443 879 343 274 203 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6873 6905 6873 Size: 32 6881 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6913 XbaI T'CTAG_A Cuts at: 3163 4249 3163 Size: 1086 5827 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6913 XmaI C'CCGG_G Cuts at: 1870 4238 1870 Size: 2368 4545 XmnI GAAnn'nnTTC Cuts at: 3054 3504 5042 3054 Size: 450 1538 4925 Fragments arranged by size: 4925 1538 450 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4721 276 Size: 53 83 186 936 3187 2468 Fragments arranged by size: 3187 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI