Restriction map for
pFN21AE3182
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# NO CUT ENZYMES ( 105 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AvrII
BamHI BbeI BbrPI BbsI BbvCI
BciVI BclI BfaI BfrI BglII
BlnI BpuAI BsaAI BsaWI BseAI
BsgI BsiWI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BsrDI BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI DraI DraIII DrdI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HindIII HpaI KasI KpnI MaeI
MfeI MroI MseI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
SbfI SexAI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp509I Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccI 42 GTCGAC GT^[AC][GT]AC
AcyI 1066 GACGCC G[GA]^CG[CT]C
AfaI 543 GTAC GT^AC
AhdI 652 GACGGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 985 GTGCAC G^TGCAC
AocI 931 CCTGAGG CC^T[ACGT]AGG
Aor51HI 365 AGCGCT AGC^GCT
ApaLI 985 GTGCAC G^TGCAC
AspEI 652 GACGGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1075 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 281 TGGCCA TGG^CCA
BcgI 87 CGAGCCCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 1158 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1158 GCTAAGC GC^T[ACGT]AGC
BsaBI 885 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1066 GACGCC G[GA]^CG[CT]C
BsaI 559 GAGACC GGTCTC(1/5)
BseRI 184 GAGGAG GAGGAG(10/8)
BsmBI 265 GAGACG CGTCTC(1/5)
BspLU11I 38 ACATGT A^CATGT
BspMI 323 ACCTGC ACCTGC(4/8)
BsrFI 230 GCCGGC [GA]^CCGG[CT]
BssHII 196 GCGCGC G^CGCGC
BssSI 238 CACGAG CACGAG(-5/-1)
BstAPI 1163 GCAGCCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 931 CCTGAGG CC^T[ACGT]AGG
CelII 1158 GCTAAGC GC^T[ACGT]AGC
Cfr10I 230 GCCGGC [GA]^CCGG[CT]
CpoI 662 CGGACCG CG^G[AT]CCG
DraII 623 GGGGCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 652 GACGGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 365 AGCGCT AGC^GCT
Eco81I 931 CCTGAGG CC^T[ACGT]AGG
EcoNI 309 CCTGCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 623 GGGGCCT [GA]G^G[ACGT]CC[CT]
Hin1I 1066 GACGCC G[GA]^CG[CT]C
HincII 42 GTCGAC GT[CT]^[GA]AC
HindII 42 GTCGAC GT[CT]^[GA]AC
HphI 872 TCACC GGTGA(8/7)
KspI 15 CCGCGG CCGC^GG
MamI 885 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1181 ACGCGT A^CGCGT
MluNI 281 TGGCCA TGG^CCA
MscI 281 TGGCCA TGG^CCA
MslI 603 CACCTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 230 GCCGGC GCC^GGC
NgoMIV 230 GCCGGC G^CCGGC
NspI 38 ACATGT [GA]CATG^[CT]
PflFI 1075 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 178 CTGCAG CTGCA^G
PvuII 1129 CAGCTG CAG^CTG
RsaI 543 GTAC GT^AC
RsrII 662 CGGACCG CG^G[AT]CCG
SacI 241 GAGCTC GAGCT^C
SacII 15 CCGCGG CCGC^GG
SalI 42 GTCGAC G^TCGAC
ScaI 542 AGTACT AGT^ACT
SfiI 616 GGCCTGGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 500 AGGCCT AGG^CCT
Tth111I 1075 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 602 CCACCTCAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 32 enzymes) #
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AflIII 38 ACATGT A^C[GA][CT]GT
1181 ACGCGT
AlwI 741 GATCC GGATC(4/5)
885 GATCC
AviII 311 TGCGCA TGC^GCA
629 TGCGCA
BanI 70 GGCACC G^G[CT][GA]CC
118 GGCACC
BglI 617 GCCTGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
992 GCCTGGCAGGC
BsiEI 232 CGGCCG CG[GA][CT]^CG
419 CGGCCG
BsmAI 265 GAGAC GTCTC(1/5)
559 GAGAC
BsrI 902 ACTGG ACTGG(1/-1)
921 CCAGT
BstXI 272 CCATCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
779 CCACCAACCTGG
BstYI 740 AGATCC [GA]^GATC[CT]
884 AGATCC
DpnII 741 GATC ^GATC
885 GATC
DsaI 15 CCGCGG C^C[GA][CT]GG
416 CCACGG
EagI 232 CGGCCG C^GGCCG
419 CGGCCG
EarI 244 CTCTTC CTCTTC(1/4)
576 CTCTTC
EclXI 232 CGGCCG C^GGCCG
419 CGGCCG
Eco52I 232 CGGCCG C^GGCCG
419 CGGCCG
EcoT14I 513 CCAAGG C^C[AT][AT]GG
770 CCAAGG
FspI 311 TGCGCA TGC^GCA
629 TGCGCA
Ksp632I 244 CTCTTC CTCTTC(1/4)
576 CTCTTC
MaeII 566 ACGT A^CGT
791 ACGT
MboI 741 GATC ^GATC
885 GATC
MflI 740 AGATCC [GA]^GATC[CT]
884 AGATCC
MspA1I 15 CCGCGG C[AC]G^C[GT]G
1129 CAGCTG
NdeII 741 GATC ^GATC
885 GATC
SapI 243 GCTCTTC GCTCTTC(1/4)
575 GCTCTTC
Sau3AI 741 GATC ^GATC
885 GATC
SfcI 178 CTGCAG C^T[GA][CT]AG
472 CTACAG
SmaI 74 CCCGGG CCC^GGG
288 CCCGGG
StyI 513 CCAAGG C^C[AT][AT]GG
770 CCAAGG
XhoII 740 AGATCC [GA]^GATC[CT]
884 AGATCC
XmaCI 74 CCCGGG C^CCGGG
288 CCCGGG
XmaI 74 CCCGGG C^CCGGG
288 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AluI AlwNI AspHI
AvaI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsoBI Bsp1286I BsrBI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI EaeI EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
ItaI MaeIII MboII MnlI MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV PleI Sau96I ScrFI SfaNI
TaqI TseI Tsp45I TspRI TthHB8I