Restriction map for
pFN21AE3182
(Linear) (Six-base) MAPSORT of: KIEE3182.seq Check: 2717 from: 1 to: 1257
KIEE3182.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 0 43 1257
Size: 43 1214
AfeI AGC'GCT
Cuts at: 0 367 1257
Size: 367 890
AflIII A'CryG_T
Cuts at: 0 38 1181 1257
Size: 38 1143 76
Fragments arranged by size:
1143 76 38
AhdI GACnn_n'nnGTC
Cuts at: 0 657 1257
Size: 657 600
AleI CACnn'nnGTG
Cuts at: 0 607 1257
Size: 607 650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 724 1257
Size: 724 533
AlwNI CAG_nnn'CTG
Cuts at: 0 480 744 1134 1257
Size: 480 264 390 123
Fragments arranged by size:
480 390 264 123
ApaLI G'TGCA_C
Cuts at: 0 985 1257
Size: 985 272
AvaI C'yCGr_G
Cuts at: 0 74 288 433 1257
Size: 74 214 145 824
Fragments arranged by size:
824 214 145 74
BanI G'GyrC_C
Cuts at: 0 70 118 1257
Size: 70 48 1139
Fragments arranged by size:
1139 70 48
BanII G_rGCy'C
Cuts at: 0 92 245 440 1257
Size: 92 153 195 817
Fragments arranged by size:
817 195 153 92
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 76 110 1257
Size: 76 34 1147
Fragments arranged by size:
1147 76 34
BglI GCCn_nnn'nGGC
Cuts at: 0 623 998 1257
Size: 623 375 259
Fragments arranged by size:
623 375 259
BlpI GC'TnA_GC
Cuts at: 0 1159 1257
Size: 1159 98
Bme1580I G_kGCm'C
Cuts at: 0 73 989 1257
Size: 73 916 268
Fragments arranged by size:
916 268 73
BmgBI CAC'GTC
Cuts at: 0 792 1257
Size: 792 465
BmrI ACTGGGnnnn_n'
Cuts at: 0 915 1257
Size: 915 342
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 924 1257
Size: 211 713 333
Fragments arranged by size:
713 333 211
BsaI GGTCTCn'nnnn_
Cuts at: 0 553 1257
Size: 553 704
BsaBI GATnn'nnATC
Cuts at: 0 889 1257
Size: 889 368
BsaHI Gr'CG_yC
Cuts at: 0 1067 1257
Size: 1067 190
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 199 1257
Size: 199 1058
BseYI C'CCAG_C
Cuts at: 0 24 64 612 676 1152 1257
Size: 24 40 548 64 476 105
Fragments arranged by size:
548 476 105 64 40 24
BsiEI CG_ry'CG
Cuts at: 0 235 422 1257
Size: 235 187 835
Fragments arranged by size:
835 235 187
BsiHKAI G_wGCw'C
Cuts at: 0 245 461 554 797 860 989 1257
Size: 245 216 93 243 63 129 268
Fragments arranged by size:
268 245 243 216 129 93 63
BsmBI CGTCTCn'nnnn_
Cuts at: 0 259 1257
Size: 259 998
Bsp1286I G_dGCh'C
Cuts at: 0 73 92 245 440 461 554 797 860
Size: 73 19 153 195 21 93 243 63
Cuts at: 860 989 1257
Size: 129 268
Fragments arranged by size:
268 243 195 153 129 93 73 63
21 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 332 1257
Size: 332 925
BsrBI CCG'CTC
Cuts at: 0 335 962 1010 1146 1257
Size: 335 627 48 136 111
Fragments arranged by size:
627 335 136 111 48
BsrFI r'CCGG_y
Cuts at: 0 230 1257
Size: 230 1027
BssHII G'CGCG_C
Cuts at: 0 196 1257
Size: 196 1061
BssSI C'ACGA_G
Cuts at: 0 238 1257
Size: 238 1019
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1169 1257
Size: 1169 88
BstXI CCAn_nnnn'nTGG
Cuts at: 0 279 786 1257
Size: 279 507 471
Fragments arranged by size:
507 471 279
BstYI r'GATC_y
Cuts at: 0 740 884 1257
Size: 740 144 373
Fragments arranged by size:
740 373 144
Bsu36I CC'TnA_GG
Cuts at: 0 932 1257
Size: 932 325
BtgI C'CryG_G
Cuts at: 0 15 416 1257
Size: 15 401 841
Fragments arranged by size:
841 401 15
BtsI GCAGTG_nn'
Cuts at: 0 552 687 987 1257
Size: 552 135 300 270
Fragments arranged by size:
552 300 270 135
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 954 989 1257
Size: 954 35 268
Fragments arranged by size:
954 268 35
EaeI y'GGCC_r
Cuts at: 0 232 269 281 389 419 1257
Size: 232 37 12 108 30 838
Fragments arranged by size:
838 232 108 37 30 12
EagI C'GGCC_G
Cuts at: 0 232 419 1257
Size: 232 187 838
Fragments arranged by size:
838 232 187
EarI CTCTTCn'nnn_
Cuts at: 0 250 582 1257
Size: 250 332 675
Fragments arranged by size:
675 332 250
EcoICRI GAG'CTC
Cuts at: 0 243 1257
Size: 243 1014
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 924 1257
Size: 211 713 333
Fragments arranged by size:
713 333 211
EcoNI CCTnn'n_nnAGG
Cuts at: 0 313 1257
Size: 313 944
EcoO109I rG'GnC_Cy
Cuts at: 0 624 1257
Size: 624 633
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 384 1257
Size: 384 873
FspI TGC'GCA
Cuts at: 0 313 631 1257
Size: 313 318 626
Fragments arranged by size:
626 318 313
HaeII r_GCGC'y
Cuts at: 0 170 369 446 1257
Size: 170 199 77 811
Fragments arranged by size:
811 199 170 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 643 675 1257
Size: 643 32 582
Fragments arranged by size:
643 582 32
HincII GTy'rAC
Cuts at: 0 44 1257
Size: 44 1213
MluI A'CGCG_T
Cuts at: 0 1181 1257
Size: 1181 76
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 641 674 1121 1257
Size: 641 33 447 136
Fragments arranged by size:
641 447 136 33
MscI TGG'CCA
Cuts at: 0 283 1257
Size: 283 974
MslI CAynn'nnrTG
Cuts at: 0 607 1257
Size: 607 650
MspA1I CmG'CkG
Cuts at: 0 17 1131 1257
Size: 17 1114 126
Fragments arranged by size:
1114 126 17
NaeI GCC'GGC
Cuts at: 0 232 1257
Size: 232 1025
NgoMIV G'CCGG_C
Cuts at: 0 230 1257
Size: 230 1027
NspI r_CATG'y
Cuts at: 0 42 1257
Size: 42 1215
PciI A'CATG_T
Cuts at: 0 38 1257
Size: 38 1219
PstI C_TGCA'G
Cuts at: 0 182 1257
Size: 182 1075
PvuII CAG'CTG
Cuts at: 0 1131 1257
Size: 1131 126
RsrII CG'GwC_CG
Cuts at: 0 663 1257
Size: 663 594
SacI G_AGCT'C
Cuts at: 0 245 1257
Size: 245 1012
SacII CC_GC'GG
Cuts at: 0 18 1257
Size: 18 1239
SalI G'TCGA_C
Cuts at: 0 42 1257
Size: 42 1215
SapI GCTCTTCn'nnn_
Cuts at: 0 250 582 1257
Size: 250 332 675
Fragments arranged by size:
675 332 250
ScaI AGT'ACT
Cuts at: 0 544 1257
Size: 544 713
SfcI C'TryA_G
Cuts at: 0 178 472 1257
Size: 178 294 785
Fragments arranged by size:
785 294 178
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 623 1257
Size: 623 634
SmaI CCC'GGG
Cuts at: 0 76 290 1257
Size: 76 214 967
Fragments arranged by size:
967 214 76
StuI AGG'CCT
Cuts at: 0 502 1257
Size: 502 755
StyI C'CwwG_G
Cuts at: 0 513 770 1257
Size: 513 257 487
Fragments arranged by size:
513 487 257
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 615 680 849 1257
Size: 615 65 169 408
Fragments arranged by size:
615 408 169 65
TatI w'GTAC_w
Cuts at: 0 542 1257
Size: 542 715
Tth111I GACn'n_nGTC
Cuts at: 0 1078 1257
Size: 1078 179
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 609 1257
Size: 609 648
XmaI C'CCGG_G
Cuts at: 0 74 288 1257
Size: 74 214 969
Fragments arranged by size:
969 214 74
Enzymes that do cut:
AccI AfeI AflIII AhdI AleI AlfI AlwNI ApaLI
AvaI BanI BanII BcgI BglI BlpI Bme1580I BmgBI
BmrI BpmI BsaI BsaBI BsaHI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtsI CspCI EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI
HaeII Hin4I HincII MluI MmeI MscI MslI MspA1I
NaeI NgoMIV NspI PciI PstI PvuII RsrII SacI
SacII SalI SapI ScaI SfcI SfiI SmaI StuI
StyI TaqII TatI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AgeI AloI
ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BbvCI BciVI BclI BfrBI BglII BmtI
BplI Bpu10I BpuEI BsaAI BsaWI BsaXI BsgI BsiWI
BsmI BspEI BspHI BsrDI BsrGI BstBI BstEII BstZ17I
BtgZI ClaI DraI DraIII DrdI EciI EcoRI EcoRV
FseI FspAI HindIII HpaI KasI KpnI MfeI NarI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI SanDI SbfI SexAI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TstI XbaI XhoI XmnI ZraI