################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AviII BseAI BseRI BsiWI BsmBI BspDI BspEI BssHII BssSI Bst1107I BstEII BstPI BstZ17I ClaI CpoI DraI EagI EarI EclXI Eco47III Eco52I EcoO65I EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I KspI MfeI MluI MroI MunI NotI NruI NsiI PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SexAI SfiI SgrAI SnaBI SpeI SphI SspI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 379 GTAGAC GT^[AC][GT]AC AflII 1999 CTTAAG C^TTAAG AspHI 1618 GAGCAC G[AT]GC[AT]^C AvaI 2900 CCCGGG C^[CT]CG[GA]G AvrII 2640 CCTAGG C^CTAGG BbrPI 484 CACGTG CAC^GTG BbsI 49 GAAGAC GAAGAC(2/6) BbvCI 2028 CCTCAGC CCTCAGC(-5/-2) BciVI 1440 GTATCC GTATCC(6/5) BfrI 1999 CTTAAG C^TTAAG BlnI 2640 CCTAGG C^CTAGG BlpI 2057 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2057 GCTCAGC GC^T[ACGT]AGC BpuAI 49 GAAGAC GAAGAC(2/6) BsaWI 29 TCCGGT [AT]^CCGG[AT] BsiEI 165 CGGTCG CG[GA][CT]^CG BsiHKAI 1618 GAGCAC G[AT]GC[AT]^C BsmI 1184 GCATTC GAATGC(1/-1) BsoBI 2900 CCCGGG C^[CT]CG[GA]G Bsp1407I 2138 TGTACA T^GTACA BspLU11I 2761 ACATGT A^CATGT BsrFI 2495 GCCGGC [GA]^CCGG[CT] BsrGI 2138 TGTACA T^GTACA BstBI 305 TTCGAA TT^CGAA CelII 2057 GCTCAGC GC^T[ACGT]AGC Cfr10I 2495 GCCGGC [GA]^CCGG[CT] DraIII 2131 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2972 GACACACCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 650 CCTTTACTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 844 GAATTC G^AATTC EcoRV 1936 GATATC GAT^ATC HgaI 130 GACGC GACGC(5/10) NaeI 2495 GCCGGC GCC^GGC NdeI 1445 CATATG CA^TATG NgoMIV 2495 GCCGGC G^CCGGC NheI 2275 GCTAGC G^CTAGC NspI 2761 ACATGT [GA]CATG^[CT] NspV 305 TTCGAA TT^CGAA PmaCI 484 CACGTG CAC^GTG PmlI 484 CACGTG CAC^GTG PpuMI 2368 GGGTCCT [GA]G^G[AT]CC[CT] SbfI 2295 CCTGCAGG CCTGCA^GG SfuI 305 TTCGAA TT^CGAA SmaI 2900 CCCGGG CCC^GGG Sse8387I 2295 CCTGCAGG CCTGCA^GG SspBI 2138 TGTACA T^GTACA StuI 1886 AGGCCT AGG^CCT XbaI 1188 TCTAGA T^CTAGA XmaCI 2900 CCCGGG C^CCGGG XmaI 2900 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AcyI 2602 GGCGCC G[GA]^CG[CT]C 2747 GGCGCC AflIII 485 ACGTGT A^C[GA][CT]GT 2761 ACATGT AhdI 151 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2917 GACATGGGGTC ApaI 2262 GGGCCC GGGCC^C 2481 GGGCCC AspEI 151 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2917 GACATGGGGTC AspI 1990 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2364 GACTGGGTC BalI 234 TGGCCA TGG^CCA 2445 TGGCCA BbeI 2602 GGCGCC GGCGC^C 2747 GGCGCC BcgI 48 CGAAGACCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1563 CGACCTCATTGC BclI 588 TGATCA T^GATCA 1449 TGATCA BglI 772 GCCATTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2618 GCCCACCAGGC BglII 206 AGATCT A^GATCT 943 AGATCT BsaAI 484 CACGTG [CT]AC^GT[GA] 1648 TACGTG BsaHI 2602 GGCGCC G[GA]^CG[CT]C 2747 GGCGCC BsgI 1828 GTGCAG GTGCAG(16/14) 2722 GTGCAG BsrDI 1569 CATTGC GCAATG(2/0) 1214 GCAATG Eam1105I 151 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2917 GACATGGGGTC FbaI 588 TGATCA T^GATCA 1449 TGATCA HaeII 2602 GGCGCC [GA]GCGC^[CT] 2747 GGCGCC Hin1I 2602 GGCGCC G[GA]^CG[CT]C 2747 GGCGCC KasI 2602 GGCGCC G^GCGCC 2747 GGCGCC MaeII 485 ACGT A^CGT 1649 ACGT MluNI 234 TGGCCA TGG^CCA 2445 TGGCCA MscI 234 TGGCCA TGG^CCA 2445 TGGCCA NarI 2602 GGCGCC GG^CGCC 2747 GGCGCC PflFI 1990 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2364 GACTGGGTC PstI 511 CTGCAG CTGCA^G 2296 CTGCAG ScaI 359 AGTACT AGT^ACT 1898 AGTACT SfcI 511 CTGCAG C^T[GA][CT]AG 2296 CTGCAG SfoI 2602 GGCGCC GGC^GCC 2747 GGCGCC Tth111I 1990 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2364 GACTGGGTC XcmI 920 CCATGGCAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1444 CCATATGATCACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AvaII BamHI BanI BanII BbvI BcnI BfaI BmyI BpmI BsaBI BsaI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BspMI BsrBI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PleI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII