(Linear) (Six-base) MAPSORT of: KIEE9629.seq Check: 355 from: 1 to: 3003 KIEE9629.orf With 182 enzymes: SgfI * July 3, 2012 19:03 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 504 3003 Size: 504 2499 AccI GT'mk_AC Cuts at: 0 380 3003 Size: 380 2623 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 245 661 1795 3003 Size: 245 416 1134 1208 Fragments arranged by size: 1208 1134 416 245 AflII C'TTAA_G Cuts at: 0 1999 3003 Size: 1999 1004 AflIII A'CryG_T Cuts at: 0 485 2761 3003 Size: 485 2276 242 Fragments arranged by size: 2276 485 242 AhdI GACnn_n'nnGTC Cuts at: 0 156 2922 3003 Size: 156 2766 81 Fragments arranged by size: 2766 156 81 AleI CACnn'nnGTG Cuts at: 0 1457 2642 2762 3003 Size: 1457 1185 120 241 Fragments arranged by size: 1457 1185 241 120 AlwNI CAG_nnn'CTG Cuts at: 0 268 426 627 669 1296 2313 3003 Size: 268 158 201 42 627 1017 690 Fragments arranged by size: 1017 690 627 268 201 158 42 ApaI G_GGCC'C Cuts at: 0 2266 2485 3003 Size: 2266 219 518 Fragments arranged by size: 2266 518 219 ApoI r'AATT_y Cuts at: 0 196 214 372 844 1804 3003 Size: 196 18 158 472 960 1199 Fragments arranged by size: 1199 960 472 196 158 18 AvaI C'yCGr_G Cuts at: 0 2900 3003 Size: 2900 103 AvrII C'CTAG_G Cuts at: 0 2640 3003 Size: 2640 363 BamHI G'GATC_C Cuts at: 0 915 1489 2633 3003 Size: 915 574 1144 370 Fragments arranged by size: 1144 915 574 370 BanI G'GyrC_C Cuts at: 0 1685 2602 2747 3003 Size: 1685 917 145 256 Fragments arranged by size: 1685 917 256 145 BanII G_rGCy'C Cuts at: 0 528 1961 2266 2485 2841 3003 Size: 528 1433 305 219 356 162 Fragments arranged by size: 1433 528 356 305 219 162 BbeI G_GCGC'C Cuts at: 0 2606 2751 3003 Size: 2606 145 252 Fragments arranged by size: 2606 252 145 BbsI GAAGACnn'nnnn_ Cuts at: 0 56 3003 Size: 56 2947 BbvCI CC'TCA_GC Cuts at: 0 2029 3003 Size: 2029 974 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 37 71 1552 1586 3003 Size: 37 34 1481 34 1417 Fragments arranged by size: 1481 1417 37 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1451 3003 Size: 1451 1552 BclI T'GATC_A Cuts at: 0 588 1449 3003 Size: 588 861 1554 Fragments arranged by size: 1554 861 588 BglI GCCn_nnn'nGGC Cuts at: 0 778 2624 3003 Size: 778 1846 379 Fragments arranged by size: 1846 778 379 BglII A'GATC_T Cuts at: 0 206 943 3003 Size: 206 737 2060 Fragments arranged by size: 2060 737 206 BlpI GC'TnA_GC Cuts at: 0 2058 3003 Size: 2058 945 Bme1580I G_kGCm'C Cuts at: 0 1688 1975 2266 2485 3003 Size: 1688 287 291 219 518 Fragments arranged by size: 1688 518 291 287 219 BmrI ACTGGGnnnn_n' Cuts at: 0 754 1890 2375 3003 Size: 754 1136 485 628 Fragments arranged by size: 1136 754 628 485 BmtI G_CTAG'C Cuts at: 0 2279 3003 Size: 2279 724 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2073 3003 Size: 2073 930 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1476 2107 2212 2807 2855 2893 3003 Size: 1476 631 105 595 48 38 110 Fragments arranged by size: 1476 631 595 110 105 48 38 Bpu10I CC'TnA_GC Cuts at: 0 1346 1382 2029 3003 Size: 1346 36 647 974 Fragments arranged by size: 1346 974 647 36 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 956 1015 1052 3003 Size: 956 59 37 1951 Fragments arranged by size: 1951 956 59 37 BsaI GGTCTCn'nnnn_ Cuts at: 0 1351 1525 1621 3003 Size: 1351 174 96 1382 Fragments arranged by size: 1382 1351 174 96 BsaAI yAC'GTr Cuts at: 0 486 1650 3003 Size: 486 1164 1353 Fragments arranged by size: 1353 1164 486 BsaBI GATnn'nnATC Cuts at: 0 224 1375 1439 3003 Size: 224 1151 64 1564 Fragments arranged by size: 1564 1151 224 64 BsaHI Gr'CG_yC Cuts at: 0 2603 2748 3003 Size: 2603 145 255 Fragments arranged by size: 2603 255 145 BsaWI w'CCGG_w Cuts at: 0 29 3003 Size: 29 2974 BseYI C'CCAG_C Cuts at: 0 1163 1504 1925 1944 2342 3003 Size: 1163 341 421 19 398 661 Fragments arranged by size: 1163 661 421 398 341 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1849 2743 3003 Size: 1849 894 260 Fragments arranged by size: 1849 894 260 BsiEI CG_ry'CG Cuts at: 0 168 3003 Size: 168 2835 BsiHKAI G_wGCw'C Cuts at: 0 1622 3003 Size: 1622 1381 BsmI GAATG_Cn' Cuts at: 0 1184 3003 Size: 1184 1819 Bsp1286I G_dGCh'C Cuts at: 0 528 1622 1688 1961 1975 2266 2485 2841 Size: 528 1094 66 273 14 291 219 356 Cuts at: 2841 3003 Size: 162 Fragments arranged by size: 1094 528 356 291 273 219 162 66 14 BspHI T'CATG_A Cuts at: 0 285 819 1409 3003 Size: 285 534 590 1594 Fragments arranged by size: 1594 590 534 285 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 161 504 1833 2443 3003 Size: 161 343 1329 610 560 Fragments arranged by size: 1329 610 560 343 161 BsrBI CCG'CTC Cuts at: 0 762 1194 2821 3003 Size: 762 432 1627 182 Fragments arranged by size: 1627 762 432 182 BsrDI GCAATG_nn' Cuts at: 0 1221 1568 3003 Size: 1221 347 1435 Fragments arranged by size: 1435 1221 347 BsrFI r'CCGG_y Cuts at: 0 2495 3003 Size: 2495 508 BsrGI T'GTAC_A Cuts at: 0 2138 3003 Size: 2138 865 BstAPI GCAn_nnn'nTGC Cuts at: 0 519 1827 2687 2957 3003 Size: 519 1308 860 270 46 Fragments arranged by size: 1308 860 519 270 46 BstBI TT'CG_AA Cuts at: 0 306 3003 Size: 306 2697 BstXI CCAn_nnnn'nTGG Cuts at: 0 81 832 1512 1903 2190 2287 2644 2968 Size: 81 751 680 391 287 97 357 324 Cuts at: 2968 3003 Size: 35 Fragments arranged by size: 751 680 391 357 324 287 97 81 35 BstYI r'GATC_y Cuts at: 0 206 915 943 1466 1489 2237 2633 3003 Size: 206 709 28 523 23 748 396 370 Fragments arranged by size: 748 709 523 396 370 206 28 23 Bsu36I CC'TnA_GG Cuts at: 0 634 692 2230 3003 Size: 634 58 1538 773 Fragments arranged by size: 1538 773 634 58 BtgI C'CryG_G Cuts at: 0 74 248 920 1401 1511 1721 2405 3003 Size: 74 174 672 481 110 210 684 598 Fragments arranged by size: 684 672 598 481 210 174 110 74 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 617 1362 3003 Size: 617 745 1641 Fragments arranged by size: 1641 745 617 BtsI GCAGTG_nn' Cuts at: 0 2112 2676 2791 3003 Size: 2112 564 115 212 Fragments arranged by size: 2112 564 212 115 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 865 900 3003 Size: 865 35 2103 Fragments arranged by size: 2103 865 35 DraIII CAC_nnn'GTG Cuts at: 0 2136 3003 Size: 2136 867 DrdI GACnn_nn'nnGTC Cuts at: 0 2978 3003 Size: 2978 25 EaeI y'GGCC_r Cuts at: 0 116 134 234 2289 2445 3003 Size: 116 18 100 2055 156 558 Fragments arranged by size: 2055 558 156 116 100 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 192 2228 3003 Size: 192 2036 775 Fragments arranged by size: 2036 775 192 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 245 661 1476 1795 2107 2212 2807 2855 Size: 245 416 815 319 312 105 595 48 Cuts at: 2855 2893 3003 Size: 38 110 Fragments arranged by size: 815 595 416 319 312 245 110 105 48 38 EcoNI CCTnn'n_nnAGG Cuts at: 0 654 3003 Size: 654 2349 EcoO109I rG'GnC_Cy Cuts at: 0 1169 2262 2263 2369 2481 2482 2572 2897 Size: 1169 1093 1 106 112 1 90 325 Cuts at: 2897 3003 Size: 106 Fragments arranged by size: 1169 1093 325 112 106 106 90 1 1 EcoRI G'AATT_C Cuts at: 0 844 3003 Size: 844 2159 EcoRV GAT'ATC Cuts at: 0 1938 3003 Size: 1938 1065 HaeII r_GCGC'y Cuts at: 0 2606 2751 3003 Size: 2606 145 252 Fragments arranged by size: 2606 252 145 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 142 174 928 960 1140 1172 1474 1506 Size: 142 32 754 32 180 32 302 32 Cuts at: 1506 2172 2204 2908 2940 3003 Size: 666 32 704 32 63 Fragments arranged by size: 754 704 666 302 180 142 63 32 32 32 32 32 32 KasI G'GCGC_C Cuts at: 0 2602 2747 3003 Size: 2602 145 256 Fragments arranged by size: 2602 256 145 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2858 3003 Size: 2858 145 MscI TGG'CCA Cuts at: 0 236 2447 3003 Size: 236 2211 556 Fragments arranged by size: 2211 556 236 MslI CAynn'nnrTG Cuts at: 0 79 230 253 830 1457 2642 2762 3003 Size: 79 151 23 577 627 1185 120 241 Fragments arranged by size: 1185 627 577 241 151 120 79 23 MspA1I CmG'CkG Cuts at: 0 728 2033 2062 3003 Size: 728 1305 29 941 Fragments arranged by size: 1305 941 728 29 NaeI GCC'GGC Cuts at: 0 2497 3003 Size: 2497 506 NarI GG'CG_CC Cuts at: 0 2603 2748 3003 Size: 2603 145 255 Fragments arranged by size: 2603 255 145 NcoI C'CATG_G Cuts at: 0 74 248 920 1401 1721 3003 Size: 74 174 672 481 320 1282 Fragments arranged by size: 1282 672 481 320 174 74 NdeI CA'TA_TG Cuts at: 0 1446 3003 Size: 1446 1557 NgoMIV G'CCGG_C Cuts at: 0 2495 3003 Size: 2495 508 NheI G'CTAG_C Cuts at: 0 2275 3003 Size: 2275 728 NspI r_CATG'y Cuts at: 0 2765 3003 Size: 2765 238 PasI CC'CwG_GG Cuts at: 0 1681 2270 2598 2610 2629 3003 Size: 1681 589 328 12 19 374 Fragments arranged by size: 1681 589 374 328 19 12 PciI A'CATG_T Cuts at: 0 2761 3003 Size: 2761 242 PflMI CCAn_nnn'nTGG Cuts at: 0 426 1216 1323 3003 Size: 426 790 107 1680 Fragments arranged by size: 1680 790 426 107 PfoI T'CCnGG_A Cuts at: 0 2240 2559 2845 3003 Size: 2240 319 286 158 Fragments arranged by size: 2240 319 286 158 PmlI CAC'GTG Cuts at: 0 486 3003 Size: 486 2517 PpuMI rG'GwC_Cy Cuts at: 0 2369 3003 Size: 2369 634 PspOMI G'GGCC_C Cuts at: 0 2262 2481 3003 Size: 2262 219 522 Fragments arranged by size: 2262 522 219 PstI C_TGCA'G Cuts at: 0 515 2300 3003 Size: 515 1785 703 Fragments arranged by size: 1785 703 515 PvuII CAG'CTG Cuts at: 0 728 2033 2062 3003 Size: 728 1305 29 941 Fragments arranged by size: 1305 941 728 29 SbfI CC_TGCA'GG Cuts at: 0 2300 3003 Size: 2300 703 ScaI AGT'ACT Cuts at: 0 361 1900 3003 Size: 361 1539 1103 Fragments arranged by size: 1539 1103 361 SfcI C'TryA_G Cuts at: 0 511 2296 3003 Size: 511 1785 707 Fragments arranged by size: 1785 707 511 SfoI GGC'GCC Cuts at: 0 2604 2749 3003 Size: 2604 145 254 Fragments arranged by size: 2604 254 145 SmaI CCC'GGG Cuts at: 0 2902 3003 Size: 2902 101 SmlI C'TyrA_G Cuts at: 0 971 994 1067 1999 3003 Size: 971 23 73 932 1004 Fragments arranged by size: 1004 971 932 73 23 StuI AGG'CCT Cuts at: 0 1888 3003 Size: 1888 1115 StyI C'CwwG_G Cuts at: 0 74 137 248 920 977 1401 1721 1889 Size: 74 63 111 672 57 424 320 168 Cuts at: 1889 2582 2640 3003 Size: 693 58 363 Fragments arranged by size: 693 672 424 363 320 168 111 74 63 58 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 595 1557 2418 3003 Size: 595 962 861 585 Fragments arranged by size: 962 861 595 585 TatI w'GTAC_w Cuts at: 0 359 1898 2138 3003 Size: 359 1539 240 865 Fragments arranged by size: 1539 865 359 240 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 429 461 552 584 3003 Size: 429 32 91 32 2419 Fragments arranged by size: 2419 429 91 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1993 2367 3003 Size: 1993 374 636 Fragments arranged by size: 1993 636 374 XbaI T'CTAG_A Cuts at: 0 1188 3003 Size: 1188 1815 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 927 1451 3003 Size: 927 524 1552 Fragments arranged by size: 1552 927 524 XmaI C'CCGG_G Cuts at: 0 2900 3003 Size: 2900 103 Enzymes that do cut: AarI AccI AcuI AflII AflIII AhdI AleI AlwNI ApaI ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI PasI PciI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SbfI ScaI SfcI SfoI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII Acc65I AclI AfeI AgeI AlfI AloI ApaLI AscI AseI AsiSI BaeI BfrBI BmgBI BsaXI BseRI BsiWI BsmBI BspEI BssHII BssSI BstEII BstZ17I ClaI DraI EagI EarI EcoICRI FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI NotI NruI NsiI PacI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XhoI XmnI ZraI