################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## AatII Acc65I AccI AclI AflII AflIII AgeI Alw44I ApaLI AseI AsnI Asp700I Asp718I AspI AvrII BalI BamHI BbrPI BbsI BbvCI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaBI BsaI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstXI BstZ17I ClaI CpoI DraI EarI EcoRV EcoT22I FbaI FseI HindIII HpaI KpnI Ksp632I MaeI MamI MfeI MluI MluNI MscI MseI MunI NcoI NdeI NheI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccIII 966 TCCGGA T^CCGGA AcsI 943 GAATTC [GA]^AATT[CT] AhdI 372 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 667 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1081 CCTCAGG CC^T[ACGT]AGG ApoI 943 GAATTC [GA]^AATT[CT] AscI 406 GGCGCGCC GG^CGCGCC AspEI 372 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1280 TGCGCA TGC^GCA BsaAI 525 CACGTA [CT]AC^GT[GA] BseAI 966 TCCGGA T^CCGGA BsgI 993 GTGCAG GTGCAG(16/14) BsmBI 364 GAGACG CGTCTC(1/5) BspEI 966 TCCGGA T^CCGGA BstAPI 666 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 369 GGTGACC G^GT[ACGT]ACC BstPI 369 GGTGACC G^GT[ACGT]ACC Bsu36I 1081 CCTCAGG CC^T[ACGT]AGG DraIII 709 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1051 GACCAGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 372 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1081 CCTCAGG CC^T[ACGT]AGG EcoNI 1077 CCTACCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 369 GGTGACC G^GT[ACGT]ACC EcoRI 943 GAATTC G^AATTC EcoT14I 1406 CCAAGG C^C[AT][AT]GG FspI 1280 TGCGCA TGC^GCA HincII 1234 GTCAAC GT[CT]^[GA]AC HindII 1234 GTCAAC GT[CT]^[GA]AC MroI 966 TCCGGA T^CCGGA MslI 1305 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NspI 959 GCATGT [GA]CATG^[CT] PflMI 1406 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 667 CAGCTG CAG^CTG SacI 303 GAGCTC GAGCT^C SfiI 435 GGCCGGGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 547 CTCAAG C^T[CT][GA]AG StyI 1406 CCAAGG C^C[AT][AT]GG Tsp509I 944 AATT ^AATT Van91I 1406 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 422 CCAGCGACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AlwI 1467 GATCC GGATC(4/5) 873 GGATC Aor51HI 554 AGCGCT AGC^GCT 1325 AGCGCT AvaII 8 GGTCC G^G[AT]CC 54 GGTCC BbeI 226 GGCGCC GGCGC^C 245 GGCGCC BcgI 1151 GCAGCAAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1394 GCATCAGCTTCG BlpI 127 GCTGAGC GC^T[ACGT]AGC 1176 GCTCAGC Bpu1102I 127 GCTGAGC GC^T[ACGT]AGC 1176 GCTCAGC BsaWI 966 TCCGGA [AT]^CCGG[AT] 1016 ACCGGA BseRI 848 CTCCTC GAGGAG(10/8) 1471 CTCCTC BspMI 373 ACCTGC ACCTGC(4/8) 701 ACCTGC BsrBI 168 CCGCTC CCGCTC(-3/-3) 391 GAGCGG BstF5I 1170 CATCC GGATG(2/0) 105 GGATG BstYI 873 GGATCT [GA]^GATC[CT] 1466 AGATCC CelII 127 GCTGAGC GC^T[ACGT]AGC 1176 GCTCAGC Eco47III 554 AGCGCT AGC^GCT 1325 AGCGCT FokI 1170 CATCC GGATG(9/13) 105 GGATG KasI 226 GGCGCC G^GCGCC 245 GGCGCC MaeII 317 ACGT A^CGT 526 ACGT MboII 1067 TCTTC GAAGA(8/7) 1229 TCTTC MflI 873 GGATCT [GA]^GATC[CT] 1466 AGATCC NarI 226 GGCGCC GG^CGCC 245 GGCGCC NlaIII 276 CATG CATG^ 960 CATG NotI 164 GCGGCCGC GC^GGCCGC 1262 GCGGCCGC NruI 474 TCGCGA TCG^CGA 852 TCGCGA PstI 703 CTGCAG CTGCA^G 1336 CTGCAG SfcI 703 CTGCAG C^T[GA][CT]AG 1336 CTGCAG SfoI 226 GGCGCC GGC^GCC 245 GGCGCC SgrAI 247 CGCCGGCG C[GA]^CCGG[CT]G 402 CGCCGGCG TaqI 1316 TCGA T^CGA 1322 TCGA TfiI 817 GAATC G^A[AT]TC 883 GATTC TthHB8I 1316 TCGA T^CGA 1322 TCGA XhoII 873 GGATCT [GA]^GATC[CT] 1466 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AccII AciI AcyI AfaI AluI ApaI AspHI AvaI BanI BanII BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssHII BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KspI MaeIII MboI MnlI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIV PleI RsaI SacII Sau3AI Sau96I ScrFI SfaNI SmaI TseI Tsp45I TspRI XmaCI XmaI