Restriction map for pFN21AB5882
 (Linear) (Six-base) MAPSORT of: KIBB5882.seq  Check: 3525  from: 1  to: 1488



KIBB5882.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 16:10  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     559     742     780    1488 
   Size:        559     183      38     708

  Fragments arranged by size: 

                 708     559     183      38
 
AfeI AGC'GCT

Cuts at:      0     556    1327    1488 
   Size:        556     771     161

  Fragments arranged by size: 

                 771     556     161
 
AhdI GACnn_n'nnGTC

Cuts at:      0     377    1488 
   Size:        377    1111
 
AlwNI CAG_nnn'CTG

Cuts at:      0     672    1488 
   Size:        672     816
 
ApaI G_GGCC'C

Cuts at:      0     265     815    1421    1488 
   Size:        265     550     606      67

  Fragments arranged by size: 

                 606     550     265      67
 
ApoI r'AATT_y

Cuts at:      0     943    1488 
   Size:        943     545
 
AscI GG'CGCG_CC

Cuts at:      0     407    1488 
   Size:        407    1081
 
AvaI C'yCGr_G

Cuts at:      0      18     203     267     336     349     413    1329    1348 
   Size:         18     185      64      69      13      64     916      19

Cuts at:   1348    1488 
   Size:        140

  Fragments arranged by size: 

                 916     185     140      69      64      64      19      18
                  13
 
BanI G'GyrC_C

Cuts at:      0     226     245     621    1287    1488 
   Size:        226      19     376     666     201

  Fragments arranged by size: 

                 666     376     226     201      19
 
BanII G_rGCy'C

Cuts at:      0     265     307     815    1190    1355    1421    1488 
   Size:        265      42     508     375     165      66      67

  Fragments arranged by size: 

                 508     375     265     165      67      66      42
 
BbeI G_GCGC'C

Cuts at:      0     230     249    1488 
   Size:        230      19    1239

  Fragments arranged by size: 

                1239     230      19
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1140    1174    1383    1417    1488 
   Size:       1140      34     209      34      71

  Fragments arranged by size: 

                1140     209      71      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     142     442     647    1488 
   Size:        142     300     205     841

  Fragments arranged by size: 

                 841     300     205     142
 
BlpI GC'TnA_GC

Cuts at:      0     128    1177    1488 
   Size:        128    1049     311

  Fragments arranged by size: 

                1049     311     128
 
Bme1580I G_kGCm'C

Cuts at:      0     265     815    1292    1421    1488 
   Size:        265     550     477     129      67

  Fragments arranged by size: 

                 550     477     265     129      67
 
BmgBI CAC'GTC

Cuts at:      0     318    1488 
   Size:        318    1170
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1017    1488 
   Size:       1017     471
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     469     523     595    1138    1483    1488 
   Size:        469      54      72     543     345       5

  Fragments arranged by size: 

                 543     469     345      72      54       5
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     532    1488 
   Size:        532     956
 
BsaAI yAC'GTr

Cuts at:      0     527    1488 
   Size:        527     961
 
BsaHI Gr'CG_yC

Cuts at:      0     227     246     398     419     953     971    1358    1488 
   Size:        227      19     152      21     534      18     387     130

  Fragments arranged by size: 

                 534     387     227     152     130      21      19      18
            
 
BsaWI w'CCGG_w

Cuts at:      0     966    1016    1488 
   Size:        966      50     472

  Fragments arranged by size: 

                 966     472      50
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     839    1462    1488 
   Size:        839     623      26

  Fragments arranged by size: 

                 839     623      26
 
BseYI C'CCAG_C

Cuts at:      0     298    1412    1488 
   Size:        298    1114      76

  Fragments arranged by size: 

                1114     298      76
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1014    1488 
   Size:       1014     474
 
BsiEI CG_ry'CG

Cuts at:      0      63     168     681    1266    1488 
   Size:         63     105     513     585     222

  Fragments arranged by size: 

                 585     513     222     105      63
 
BsiHKAI G_wGCw'C

Cuts at:      0     307    1016    1223    1488 
   Size:        307     709     207     265

  Fragments arranged by size: 

                 709     307     265     207
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     358    1488 
   Size:        358    1130
 
Bsp1286I G_dGCh'C

Cuts at:      0     265     307     815    1016    1190    1223    1292    1355 
   Size:        265      42     508     201     174      33      69      63

Cuts at:   1355    1421    1488 
   Size:         66      67

  Fragments arranged by size: 

                 508     265     201     174      69      67      66      63
                  42      33
 
BspEI T'CCGG_A

Cuts at:      0     966    1488 
   Size:        966     522
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     382     710    1488 
   Size:        382     328     778

  Fragments arranged by size: 

                 778     382     328
 
BsrBI CCG'CTC

Cuts at:      0     170     393    1488 
   Size:        170     223    1095

  Fragments arranged by size: 

                1095     223     170
 
BsrFI r'CCGG_y

Cuts at:      0     132     248     292     403     453     800    1488 
   Size:        132     116      44     111      50     347     688

  Fragments arranged by size: 

                 688     347     132     116     111      50      44
 
BssHII G'CGCG_C

Cuts at:      0     252     407     481     627    1488 
   Size:        252     155      74     146     861

  Fragments arranged by size: 

                 861     252     155     146      74
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     672    1488 
   Size:        672     816
 
BstEII G'GTnAC_C

Cuts at:      0     369    1488 
   Size:        369    1119
 
BstYI r'GATC_y

Cuts at:      0     873    1466    1488 
   Size:        873     593      22

  Fragments arranged by size: 

                 873     593      22
 
Bsu36I CC'TnA_GG

Cuts at:      0    1082    1488 
   Size:       1082     406
 
BtgI C'CryG_G

Cuts at:      0     139     193     591     713    1212    1488 
   Size:        139      54     398     122     499     276

  Fragments arranged by size: 

                 499     398     276     139     122      54
 
BtsI GCAGTG_nn'

Cuts at:      0     218     929    1488 
   Size:        218     711     559

  Fragments arranged by size: 

                 711     559     218
 
DraIII CAC_nnn'GTG

Cuts at:      0     714    1488 
   Size:        714     774
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1057    1488 
   Size:       1057     431
 
EaeI y'GGCC_r

Cuts at:      0     165     434     648     678    1263    1488 
   Size:        165     269     214      30     585     225

  Fragments arranged by size: 

                 585     269     225     214     165      30
 
EagI C'GGCC_G

Cuts at:      0     165     678    1263    1488 
   Size:        165     513     585     225

  Fragments arranged by size: 

                 585     513     225     165
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     169     170     337     483     628     812    1488 
   Size:        169       1     167     146     145     184     676

  Fragments arranged by size: 

                 676     184     169     167     146     145       1
 
EcoICRI GAG'CTC

Cuts at:      0     305    1488 
   Size:        305    1183
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     469     523     559     595     742     780    1138    1483 
   Size:        469      54      36      36     147      38     358     345

Cuts at:   1483    1488 
   Size:          5

  Fragments arranged by size: 

                 469     358     345     147      54      38      36      36
                   5
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1081    1488 
   Size:       1081     407
 
EcoO109I rG'GnC_Cy

Cuts at:      0     261     262    1417    1418    1488 
   Size:        261       1    1155       1      70

  Fragments arranged by size: 

                1155     261      70       1       1
 
EcoRI G'AATT_C

Cuts at:      0     943    1488 
   Size:        943     545
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1179    1488 
   Size:       1179     309
 
FspI TGC'GCA

Cuts at:      0    1282    1488 
   Size:       1282     206
 
HaeII r_GCGC'y

Cuts at:      0     230     249     299     558    1329    1488 
   Size:        230      19      50     259     771     159

  Fragments arranged by size: 

                 771     259     230     159      50      19
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     363     395    1488 
   Size:        363      32    1093

  Fragments arranged by size: 

                1093     363      32
 
HincII GTy'rAC

Cuts at:      0    1236    1488 
   Size:       1236     252
 
KasI G'GCGC_C

Cuts at:      0     226     245    1488 
   Size:        226      19    1243

  Fragments arranged by size: 

                1243     226      19
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     260    1488 
   Size:        260    1228
 
MslI CAynn'nnrTG

Cuts at:      0    1309    1488 
   Size:       1309     179
 
MspA1I CmG'CkG

Cuts at:      0      80     141     195     593     669    1091    1214    1488 
   Size:         80      61      54     398      76     422     123     274

  Fragments arranged by size: 

                 422     398     274     123      80      76      61      54
            
 
NaeI GCC'GGC

Cuts at:      0     134     250     294     405     455     802    1488 
   Size:        134     116      44     111      50     347     686

  Fragments arranged by size: 

                 686     347     134     116     111      50      44
 
NarI GG'CG_CC

Cuts at:      0     227     246    1488 
   Size:        227      19    1242

  Fragments arranged by size: 

                1242     227      19
 
NgoMIV G'CCGG_C

Cuts at:      0     132     248     292     403     453     800    1488 
   Size:        132     116      44     111      50     347     688

  Fragments arranged by size: 

                 688     347     132     116     111      50      44
 
NotI GC'GGCC_GC

Cuts at:      0     165    1263    1488 
   Size:        165    1098     225

  Fragments arranged by size: 

                1098     225     165
 
NruI TCG'CGA

Cuts at:      0     476     854    1488 
   Size:        476     378     634

  Fragments arranged by size: 

                 634     476     378
 
NspI r_CATG'y

Cuts at:      0     963    1488 
   Size:        963     525
 
PasI CC'CwG_GG

Cuts at:      0     231    1488 
   Size:        231    1257
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1412    1488 
   Size:       1412      76
 
PspOMI G'GGCC_C

Cuts at:      0     261     811    1417    1488 
   Size:        261     550     606      71

  Fragments arranged by size: 

                 606     550     261      71
 
PstI C_TGCA'G

Cuts at:      0     707    1340    1488 
   Size:        707     633     148

  Fragments arranged by size: 

                 707     633     148
 
PvuII CAG'CTG

Cuts at:      0     669    1488 
   Size:        669     819
 
SacI G_AGCT'C

Cuts at:      0     307    1488 
   Size:        307    1181
 
SacII CC_GC'GG

Cuts at:      0     142     196     594    1215    1488 
   Size:        142      54     398     621     273

  Fragments arranged by size: 

                 621     398     273     142      54
 
SfcI C'TryA_G

Cuts at:      0     703    1336    1488 
   Size:        703     633     152

  Fragments arranged by size: 

                 703     633     152
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     442    1488 
   Size:        442    1046
 
SfoI GGC'GCC

Cuts at:      0     228     247    1488 
   Size:        228      19    1241

  Fragments arranged by size: 

                1241     228      19
 
SgrAI Cr'CCGG_yG

Cuts at:      0     248     403    1488 
   Size:        248     155    1085

  Fragments arranged by size: 

                1085     248     155
 
SmaI CCC'GGG

Cuts at:      0     205     338     415    1488 
   Size:        205     133      77    1073

  Fragments arranged by size: 

                1073     205     133      77
 
SmlI C'TyrA_G

Cuts at:      0     547    1488 
   Size:        547     941
 
SrfI GCCC'GGGC

Cuts at:      0     338    1488 
   Size:        338    1150
 
StyI C'CwwG_G

Cuts at:      0    1406    1488 
   Size:       1406      82
 
TatI w'GTAC_w

Cuts at:      0     860    1488 
   Size:        860     628
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     429    1488 
   Size:        429    1059
 
XmaI C'CCGG_G

Cuts at:      0     203     336     413    1488 
   Size:        203     133      77    1075

  Fragments arranged by size: 

                1075     203     133      77
 

 Enzymes that do cut:

     AcuI     AfeI     AhdI    AlwNI     ApaI     ApoI     AscI     AvaI
     BanI    BanII     BbeI     BcgI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BpmI    BpuEI    BsaAI    BsaHI    BsaWI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI
    BsrFI   BssHII   BstAPI   BstEII    BstYI   Bsu36I     BtgI     BtsI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI
     MmeI     MslI   MspA1I     NaeI     NarI   NgoMIV     NotI     NruI
     NspI     PasI    PflMI   PspOMI     PstI    PvuII     SacI    SacII
     SfcI     SfiI     SfoI    SgrAI     SmaI     SmlI     SrfI     StyI
     TatI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AleI     AlfI     AloI    ApaLI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbsI    BbvCI    BciVI     BclI    BfrBI    BglII     BmtI
     BplI   Bpu10I     BsaI    BsaBI    BsaXI    BsiWI     BsmI    BspHI
    BsrDI    BsrGI    BssSI    BstBI    BstXI  BstZ17I    BtgZI     ClaI
    CspCI     DraI     EarI    EcoRV     FseI    FspAI  HindIII     HpaI
     KpnI     MfeI     MluI     MscI     NcoI     NdeI     NheI     NsiI
     PacI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SgfI    SnaBI     SpeI     SphI     SspI
     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     XmnI
     ZraI
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