(Linear) (Six-base) MAPSORT of: KIBB5882.seq Check: 3525 from: 1 to: 1488 KIBB5882.orf With 182 enzymes: SgfI * July 3, 2012 16:10 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 559 742 780 1488 Size: 559 183 38 708 Fragments arranged by size: 708 559 183 38 AfeI AGC'GCT Cuts at: 0 556 1327 1488 Size: 556 771 161 Fragments arranged by size: 771 556 161 AhdI GACnn_n'nnGTC Cuts at: 0 377 1488 Size: 377 1111 AlwNI CAG_nnn'CTG Cuts at: 0 672 1488 Size: 672 816 ApaI G_GGCC'C Cuts at: 0 265 815 1421 1488 Size: 265 550 606 67 Fragments arranged by size: 606 550 265 67 ApoI r'AATT_y Cuts at: 0 943 1488 Size: 943 545 AscI GG'CGCG_CC Cuts at: 0 407 1488 Size: 407 1081 AvaI C'yCGr_G Cuts at: 0 18 203 267 336 349 413 1329 1348 Size: 18 185 64 69 13 64 916 19 Cuts at: 1348 1488 Size: 140 Fragments arranged by size: 916 185 140 69 64 64 19 18 13 BanI G'GyrC_C Cuts at: 0 226 245 621 1287 1488 Size: 226 19 376 666 201 Fragments arranged by size: 666 376 226 201 19 BanII G_rGCy'C Cuts at: 0 265 307 815 1190 1355 1421 1488 Size: 265 42 508 375 165 66 67 Fragments arranged by size: 508 375 265 165 67 66 42 BbeI G_GCGC'C Cuts at: 0 230 249 1488 Size: 230 19 1239 Fragments arranged by size: 1239 230 19 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1140 1174 1383 1417 1488 Size: 1140 34 209 34 71 Fragments arranged by size: 1140 209 71 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 142 442 647 1488 Size: 142 300 205 841 Fragments arranged by size: 841 300 205 142 BlpI GC'TnA_GC Cuts at: 0 128 1177 1488 Size: 128 1049 311 Fragments arranged by size: 1049 311 128 Bme1580I G_kGCm'C Cuts at: 0 265 815 1292 1421 1488 Size: 265 550 477 129 67 Fragments arranged by size: 550 477 265 129 67 BmgBI CAC'GTC Cuts at: 0 318 1488 Size: 318 1170 BmrI ACTGGGnnnn_n' Cuts at: 0 1017 1488 Size: 1017 471 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 469 523 595 1138 1483 1488 Size: 469 54 72 543 345 5 Fragments arranged by size: 543 469 345 72 54 5 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 532 1488 Size: 532 956 BsaAI yAC'GTr Cuts at: 0 527 1488 Size: 527 961 BsaHI Gr'CG_yC Cuts at: 0 227 246 398 419 953 971 1358 1488 Size: 227 19 152 21 534 18 387 130 Fragments arranged by size: 534 387 227 152 130 21 19 18 BsaWI w'CCGG_w Cuts at: 0 966 1016 1488 Size: 966 50 472 Fragments arranged by size: 966 472 50 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 839 1462 1488 Size: 839 623 26 Fragments arranged by size: 839 623 26 BseYI C'CCAG_C Cuts at: 0 298 1412 1488 Size: 298 1114 76 Fragments arranged by size: 1114 298 76 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1014 1488 Size: 1014 474 BsiEI CG_ry'CG Cuts at: 0 63 168 681 1266 1488 Size: 63 105 513 585 222 Fragments arranged by size: 585 513 222 105 63 BsiHKAI G_wGCw'C Cuts at: 0 307 1016 1223 1488 Size: 307 709 207 265 Fragments arranged by size: 709 307 265 207 BsmBI CGTCTCn'nnnn_ Cuts at: 0 358 1488 Size: 358 1130 Bsp1286I G_dGCh'C Cuts at: 0 265 307 815 1016 1190 1223 1292 1355 Size: 265 42 508 201 174 33 69 63 Cuts at: 1355 1421 1488 Size: 66 67 Fragments arranged by size: 508 265 201 174 69 67 66 63 42 33 BspEI T'CCGG_A Cuts at: 0 966 1488 Size: 966 522 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 382 710 1488 Size: 382 328 778 Fragments arranged by size: 778 382 328 BsrBI CCG'CTC Cuts at: 0 170 393 1488 Size: 170 223 1095 Fragments arranged by size: 1095 223 170 BsrFI r'CCGG_y Cuts at: 0 132 248 292 403 453 800 1488 Size: 132 116 44 111 50 347 688 Fragments arranged by size: 688 347 132 116 111 50 44 BssHII G'CGCG_C Cuts at: 0 252 407 481 627 1488 Size: 252 155 74 146 861 Fragments arranged by size: 861 252 155 146 74 BstAPI GCAn_nnn'nTGC Cuts at: 0 672 1488 Size: 672 816 BstEII G'GTnAC_C Cuts at: 0 369 1488 Size: 369 1119 BstYI r'GATC_y Cuts at: 0 873 1466 1488 Size: 873 593 22 Fragments arranged by size: 873 593 22 Bsu36I CC'TnA_GG Cuts at: 0 1082 1488 Size: 1082 406 BtgI C'CryG_G Cuts at: 0 139 193 591 713 1212 1488 Size: 139 54 398 122 499 276 Fragments arranged by size: 499 398 276 139 122 54 BtsI GCAGTG_nn' Cuts at: 0 218 929 1488 Size: 218 711 559 Fragments arranged by size: 711 559 218 DraIII CAC_nnn'GTG Cuts at: 0 714 1488 Size: 714 774 DrdI GACnn_nn'nnGTC Cuts at: 0 1057 1488 Size: 1057 431 EaeI y'GGCC_r Cuts at: 0 165 434 648 678 1263 1488 Size: 165 269 214 30 585 225 Fragments arranged by size: 585 269 225 214 165 30 EagI C'GGCC_G Cuts at: 0 165 678 1263 1488 Size: 165 513 585 225 Fragments arranged by size: 585 513 225 165 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 169 170 337 483 628 812 1488 Size: 169 1 167 146 145 184 676 Fragments arranged by size: 676 184 169 167 146 145 1 EcoICRI GAG'CTC Cuts at: 0 305 1488 Size: 305 1183 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 469 523 559 595 742 780 1138 1483 Size: 469 54 36 36 147 38 358 345 Cuts at: 1483 1488 Size: 5 Fragments arranged by size: 469 358 345 147 54 38 36 36 5 EcoNI CCTnn'n_nnAGG Cuts at: 0 1081 1488 Size: 1081 407 EcoO109I rG'GnC_Cy Cuts at: 0 261 262 1417 1418 1488 Size: 261 1 1155 1 70 Fragments arranged by size: 1155 261 70 1 1 EcoRI G'AATT_C Cuts at: 0 943 1488 Size: 943 545 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1179 1488 Size: 1179 309 FspI TGC'GCA Cuts at: 0 1282 1488 Size: 1282 206 HaeII r_GCGC'y Cuts at: 0 230 249 299 558 1329 1488 Size: 230 19 50 259 771 159 Fragments arranged by size: 771 259 230 159 50 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 363 395 1488 Size: 363 32 1093 Fragments arranged by size: 1093 363 32 HincII GTy'rAC Cuts at: 0 1236 1488 Size: 1236 252 KasI G'GCGC_C Cuts at: 0 226 245 1488 Size: 226 19 1243 Fragments arranged by size: 1243 226 19 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 260 1488 Size: 260 1228 MslI CAynn'nnrTG Cuts at: 0 1309 1488 Size: 1309 179 MspA1I CmG'CkG Cuts at: 0 80 141 195 593 669 1091 1214 1488 Size: 80 61 54 398 76 422 123 274 Fragments arranged by size: 422 398 274 123 80 76 61 54 NaeI GCC'GGC Cuts at: 0 134 250 294 405 455 802 1488 Size: 134 116 44 111 50 347 686 Fragments arranged by size: 686 347 134 116 111 50 44 NarI GG'CG_CC Cuts at: 0 227 246 1488 Size: 227 19 1242 Fragments arranged by size: 1242 227 19 NgoMIV G'CCGG_C Cuts at: 0 132 248 292 403 453 800 1488 Size: 132 116 44 111 50 347 688 Fragments arranged by size: 688 347 132 116 111 50 44 NotI GC'GGCC_GC Cuts at: 0 165 1263 1488 Size: 165 1098 225 Fragments arranged by size: 1098 225 165 NruI TCG'CGA Cuts at: 0 476 854 1488 Size: 476 378 634 Fragments arranged by size: 634 476 378 NspI r_CATG'y Cuts at: 0 963 1488 Size: 963 525 PasI CC'CwG_GG Cuts at: 0 231 1488 Size: 231 1257 PflMI CCAn_nnn'nTGG Cuts at: 0 1412 1488 Size: 1412 76 PspOMI G'GGCC_C Cuts at: 0 261 811 1417 1488 Size: 261 550 606 71 Fragments arranged by size: 606 550 261 71 PstI C_TGCA'G Cuts at: 0 707 1340 1488 Size: 707 633 148 Fragments arranged by size: 707 633 148 PvuII CAG'CTG Cuts at: 0 669 1488 Size: 669 819 SacI G_AGCT'C Cuts at: 0 307 1488 Size: 307 1181 SacII CC_GC'GG Cuts at: 0 142 196 594 1215 1488 Size: 142 54 398 621 273 Fragments arranged by size: 621 398 273 142 54 SfcI C'TryA_G Cuts at: 0 703 1336 1488 Size: 703 633 152 Fragments arranged by size: 703 633 152 SfiI GGCCn_nnn'nGGCC Cuts at: 0 442 1488 Size: 442 1046 SfoI GGC'GCC Cuts at: 0 228 247 1488 Size: 228 19 1241 Fragments arranged by size: 1241 228 19 SgrAI Cr'CCGG_yG Cuts at: 0 248 403 1488 Size: 248 155 1085 Fragments arranged by size: 1085 248 155 SmaI CCC'GGG Cuts at: 0 205 338 415 1488 Size: 205 133 77 1073 Fragments arranged by size: 1073 205 133 77 SmlI C'TyrA_G Cuts at: 0 547 1488 Size: 547 941 SrfI GCCC'GGGC Cuts at: 0 338 1488 Size: 338 1150 StyI C'CwwG_G Cuts at: 0 1406 1488 Size: 1406 82 TatI w'GTAC_w Cuts at: 0 860 1488 Size: 860 628 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 429 1488 Size: 429 1059 XmaI C'CCGG_G Cuts at: 0 203 336 413 1488 Size: 203 133 77 1075 Fragments arranged by size: 1075 203 133 77 Enzymes that do cut: AcuI AfeI AhdI AlwNI ApaI ApoI AscI AvaI BanI BanII BbeI BcgI BglI BlpI Bme1580I BmgBI BmrI BpmI BpuEI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssHII BstAPI BstEII BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI MmeI MslI MspA1I NaeI NarI NgoMIV NotI NruI NspI PasI PflMI PspOMI PstI PvuII SacI SacII SfcI SfiI SfoI SgrAI SmaI SmlI SrfI StyI TatI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AleI AlfI AloI ApaLI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BglII BmtI BplI Bpu10I BsaI BsaBI BsaXI BsiWI BsmI BspHI BsrDI BsrGI BssSI BstBI BstXI BstZ17I BtgZI ClaI CspCI DraI EarI EcoRV FseI FspAI HindIII HpaI KpnI MfeI MluI MscI NcoI NdeI NheI NsiI PacI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI SgfI SnaBI SpeI SphI SspI StuI SwaI TaqII TstI Tth111I XbaI XhoI XmnI ZraI