(Circular) (Six-base) MAPSORT of: pFN21AE4271.seq Check: 5632 from: 1 to: 8116 pFN21AE4271 8116 bp With 182 enzymes: SgfI * July 6, 2012 17:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5926 278 Size: 53 83 186 936 4390 2468 Fragments arranged by size: 4390 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2093 2857 4294 5459 1937 Size: 156 764 1437 1165 4594 Fragments arranged by size: 4594 1437 1165 764 156 Acc65I G'GTAC_C Cuts at: 5437 5437 Size: 8116 AclI AA'CG_TT Cuts at: 1563 6243 6616 1563 Size: 4680 373 3063 Fragments arranged by size: 4680 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2574 6179 7563 7831 1632 Size: 942 3605 1384 268 1917 Fragments arranged by size: 3605 1917 1384 942 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7919 AflIII A'CryG_T Cuts at: 1203 7015 7992 1203 Size: 5812 977 1327 Fragments arranged by size: 5812 1327 977 AgeI A'CCGG_T Cuts at: 6947 6947 Size: 8116 AhdI GACnn_n'nnGTC Cuts at: 6845 6845 Size: 8116 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8116 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2754 1184 Size: 1570 6546 AlwNI CAG_nnn'CTG Cuts at: 2057 2165 5859 7431 2057 Size: 108 3694 1572 2742 Fragments arranged by size: 3694 2742 1572 108 ApaLI G'TGCA_C Cuts at: 6173 7329 6173 Size: 1156 6960 ApoI r'AATT_y Cuts at: 1493 2420 2638 3360 3539 3574 3602 3956 4026 Size: 927 218 722 179 35 28 354 70 Cuts at: 4026 4158 4442 5247 5425 5651 5803 7912 1493 Size: 132 284 805 178 226 152 2109 1697 Fragments arranged by size: 2109 1697 927 805 722 354 284 226 218 179 178 152 132 70 35 28 AseI AT'TA_AT Cuts at: 160 1032 2574 3615 5291 6670 160 Size: 872 1542 1041 1676 1379 1606 Fragments arranged by size: 1676 1606 1542 1379 1041 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8116 AvaI C'yCGr_G Cuts at: 1870 1943 2805 5441 1870 Size: 73 862 2636 4545 Fragments arranged by size: 4545 2636 862 73 BamHI G'GATC_C Cuts at: 5128 5446 5813 5128 Size: 318 367 7431 Fragments arranged by size: 7431 367 318 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4593 5437 6892 618 Size: 325 217 627 2806 844 1455 1842 Fragments arranged by size: 2806 1842 1455 844 627 325 217 BanII G_rGCy'C Cuts at: 1391 2814 4715 5435 1391 Size: 1423 1901 720 4072 Fragments arranged by size: 4072 1901 1423 720 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2516 928 Size: 971 617 6528 Fragments arranged by size: 6528 971 617 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6307 6341 1903 Size: 34 4370 34 3678 Fragments arranged by size: 4370 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6008 7218 6008 Size: 1210 6906 BclI T'GATC_A Cuts at: 1548 1548 Size: 8116 BfrBI ATG'CAT Cuts at: 4189 5344 4189 Size: 1155 6961 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2180 6727 136 Size: 107 122 71 631 605 508 4547 1525 Fragments arranged by size: 4547 1525 631 605 508 122 107 71 BglII A'GATC_T Cuts at: 8111 8111 Size: 8116 BlpI GC'TnA_GC Cuts at: 2050 4527 5533 2050 Size: 2477 1006 4633 Fragments arranged by size: 4633 2477 1006 Bme1580I G_kGCm'C Cuts at: 1790 6177 7333 1790 Size: 4387 1156 2573 Fragments arranged by size: 4387 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8116 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5847 6805 442 Size: 416 53 83 399 24 4430 958 1753 Fragments arranged by size: 4430 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8116 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2029 5262 6776 1239 Size: 790 3233 1514 2579 Fragments arranged by size: 3233 2579 1514 790 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5006 5300 6241 7106 7404 5006 Size: 294 941 865 298 5718 Fragments arranged by size: 5718 941 865 298 294 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2263 6779 882 Size: 617 764 4516 2219 Fragments arranged by size: 4516 2219 764 617 BsaAI yAC'GTr Cuts at: 493 1204 2239 3851 5907 493 Size: 711 1035 1612 2056 2702 Fragments arranged by size: 2702 2056 1612 1035 711 BsaBI GATnn'nnATC Cuts at: 3036 4742 8110 3036 Size: 1706 3368 3042 Fragments arranged by size: 3368 3042 1706 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5923 6305 6940 275 Size: 53 83 186 936 4390 382 635 1451 Fragments arranged by size: 4390 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4008 4688 6549 6947 7221 7368 7779 4008 Size: 680 1861 398 274 147 411 4345 Fragments arranged by size: 4345 1861 680 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3018 3048 1178 Size: 30 165 30 1615 30 6246 Fragments arranged by size: 6246 1615 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2107 2110 4028 5390 1186 Size: 921 3 1918 1362 3912 Fragments arranged by size: 3912 1918 1362 921 3 BseYI C'CCAG_C Cuts at: 2194 2203 2223 3747 4615 7319 7815 2194 Size: 9 20 1524 868 2704 496 2495 Fragments arranged by size: 2704 2495 1524 868 496 20 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2151 3203 3368 4212 1163 Size: 573 415 1052 165 844 5067 Fragments arranged by size: 5067 1052 844 573 415 165 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2175 2184 5566 6327 7355 1540 Size: 275 186 174 9 3382 761 1028 2301 Fragments arranged by size: 3382 2301 1028 761 275 186 174 9 BsiHKAI G_wGCw'C Cuts at: 2814 4715 4952 6177 6262 7333 2814 Size: 1901 237 1225 85 1071 3597 Fragments arranged by size: 3597 1901 1225 1071 237 85 BsmI GAATG_Cn' Cuts at: 2545 4191 5627 5720 2545 Size: 1646 1436 93 4941 Fragments arranged by size: 4941 1646 1436 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3715 3715 Size: 8116 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2814 4715 4952 5435 6177 6262 7333 Size: 399 1024 1901 237 483 742 85 1071 Cuts at: 7333 1391 Size: 2174 Fragments arranged by size: 2174 1901 1071 1024 742 483 399 237 85 BspEI T'CCGG_A Cuts at: 4008 7779 4008 Size: 3771 4345 BspHI T'CATG_A Cuts at: 2632 4954 6005 7735 2632 Size: 2322 1051 1730 3013 Fragments arranged by size: 3013 2322 1730 1051 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3982 5367 5471 844 Size: 3138 1385 104 3489 Fragments arranged by size: 3489 3138 1385 104 BsrBI CCG'CTC Cuts at: 1394 1823 6003 1394 Size: 429 4180 3507 Fragments arranged by size: 4180 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4968 6611 6785 65 Size: 1183 3720 1643 174 1396 Fragments arranged by size: 3720 1643 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6760 6947 1700 Size: 110 4950 187 2869 Fragments arranged by size: 4950 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4555 96 Size: 4459 3657 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8116 BssSI C'ACGA_G Cuts at: 6176 7188 6176 Size: 1012 7104 BstAPI GCAn_nnn'nTGC Cuts at: 794 2165 2540 2798 794 Size: 1371 375 258 6112 Fragments arranged by size: 6112 1371 375 258 BstBI TT'CG_AA Cuts at: 2561 6920 2561 Size: 4359 3757 BstEII G'GTnAC_C Cuts at: 1184 3742 1184 Size: 2558 5558 BstXI CCAn_nnnn'nTGG Cuts at: 4305 4305 Size: 8116 BstYI r'GATC_y Cuts at: 1972 4179 5128 5446 5813 6198 6215 7667 7753 Size: 2207 949 318 367 385 17 1452 86 Cuts at: 7753 7765 8111 1972 Size: 12 346 1977 Fragments arranged by size: 2207 1977 1452 949 385 367 346 318 86 17 12 BstZ17I GTA'TAC Cuts at: 2858 4295 2858 Size: 1437 6679 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 7564 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7377 Fragments arranged by size: 7377 683 56 BtsI GCAGTG_nn' Cuts at: 4420 4482 5632 6426 6446 4420 Size: 62 1150 794 20 6090 Fragments arranged by size: 6090 1150 794 62 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8116 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8081 DraI TTT'AAA Cuts at: 3580 4114 5420 3580 Size: 534 1306 6276 Fragments arranged by size: 6276 1306 534 DraIII CAC_nnn'GTG Cuts at: 4600 5077 4600 Size: 477 7639 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7123 809 Size: 507 5807 1802 Fragments arranged by size: 5807 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2172 2181 4515 Size: 54 1425 50 275 18 342 9 2334 Cuts at: 4515 5563 6452 8081 8 Size: 1048 889 1629 43 Fragments arranged by size: 2334 1629 1425 1048 889 342 275 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2172 2181 5563 1537 Size: 275 360 9 3382 4090 Fragments arranged by size: 4090 3382 360 275 9 EarI CTCTTCn'nnn_ Cuts at: 1353 2261 4933 5412 6046 1353 Size: 908 2672 479 634 3423 Fragments arranged by size: 3423 2672 908 634 479 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5028 6703 7077 7223 387 Size: 1064 138 3439 1675 374 146 1280 Fragments arranged by size: 3439 1675 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2812 4713 2812 Size: 1901 6215 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2029 2574 5262 6179 6776 7563 7831 Size: 393 397 545 2688 917 597 787 268 Cuts at: 7831 1239 Size: 1524 Fragments arranged by size: 2688 1524 917 787 597 545 397 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3192 4517 3192 Size: 1325 6791 EcoRI G'AATT_C Cuts at: 2420 3360 3539 5425 5803 2420 Size: 940 179 1886 378 4733 Fragments arranged by size: 4733 1886 940 378 179 EcoRV GAT'ATC Cuts at: 2518 2518 Size: 8116 FspI TGC'GCA Cuts at: 6622 6622 Size: 8116 HaeII r_GCGC'y Cuts at: 1678 2157 2192 2201 3320 7263 1678 Size: 479 35 9 1119 3943 2531 Fragments arranged by size: 3943 2531 1119 479 35 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2452 2484 3779 3811 3826 Size: 32 135 32 880 32 1295 32 15 Cuts at: 3826 3858 4936 4968 4994 5026 5088 5120 6757 Size: 32 1078 32 26 32 62 32 1637 Cuts at: 6757 6789 6831 6863 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1637 1295 1078 880 135 62 42 32 32 32 32 32 32 32 32 32 32 26 15 HincII GTy'rAC Cuts at: 669 1657 1938 3448 3484 4498 5460 5706 669 Size: 988 281 1510 36 1014 962 246 3079 Fragments arranged by size: 3079 1510 1014 988 962 281 246 36 HindIII A'AGCT_T Cuts at: 748 748 Size: 8116 HpaI GTT'AAC Cuts at: 3484 5706 3484 Size: 2222 5894 KpnI G_GTAC'C Cuts at: 5441 5441 Size: 8116 MfeI C'AATT_G Cuts at: 4788 5715 4788 Size: 927 7189 MluI A'CGCG_T Cuts at: 7992 7992 Size: 8116 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2341 4297 7230 7414 1305 Size: 1036 1956 2933 184 2007 Fragments arranged by size: 2933 2007 1956 1036 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4517 10 Size: 54 1425 343 2685 3609 Fragments arranged by size: 3609 2685 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2717 2736 4817 6074 6433 6592 518 Size: 648 1551 19 2081 1257 359 159 2042 Fragments arranged by size: 2081 2042 1551 1257 648 359 159 19 MspA1I CmG'CkG Cuts at: 1606 2162 2171 2180 2227 5532 6209 7357 7602 Size: 556 9 9 47 3305 677 1148 245 Cuts at: 7602 1606 Size: 2120 Fragments arranged by size: 3305 2120 1148 677 556 245 47 9 9 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7564 NdeI CA'TA_TG Cuts at: 387 387 Size: 8116 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8116 NotI GC'GGCC_GC Cuts at: 2172 2181 5563 2172 Size: 9 3382 4725 Fragments arranged by size: 4725 3382 9 NsiI A_TGCA'T Cuts at: 4191 5346 4191 Size: 1155 6961 NspI r_CATG'y Cuts at: 1132 1229 5474 7019 1132 Size: 97 4245 1545 2229 Fragments arranged by size: 4245 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8116 PciI A'CATG_T Cuts at: 7015 7015 Size: 8116 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8116 PfoI T'CCnGG_A Cuts at: 2984 3219 4389 5239 2984 Size: 235 1170 850 5861 Fragments arranged by size: 5861 1170 850 235 PmeI GTTT'AAAC Cuts at: 5420 5420 Size: 8116 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6185 6217 7698 7730 6185 Size: 32 1481 32 6571 Fragments arranged by size: 6571 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8116 PsiI TTA'TAA Cuts at: 5686 5686 Size: 8116 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3786 3818 3786 Size: 32 8084 PstI C_TGCA'G Cuts at: 830 2161 2170 2475 2944 3270 4415 4547 4777 Size: 1331 9 305 469 326 1145 132 230 Cuts at: 4777 4831 5364 5468 830 Size: 54 533 104 3478 Fragments arranged by size: 3478 1331 1145 533 469 326 305 230 132 104 54 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8116 PvuII CAG'CTG Cuts at: 2227 2227 Size: 8116 RsrII CG'GwC_CG Cuts at: 4011 4011 Size: 8116 SacI G_AGCT'C Cuts at: 2814 4715 2814 Size: 1901 6215 SalI G'TCGA_C Cuts at: 1936 5458 1936 Size: 3522 4594 SbfI CC_TGCA'GG Cuts at: 5468 5468 Size: 8116 ScaI AGT'ACT Cuts at: 6364 8076 6364 Size: 1712 6404 SexAI A'CCwGG_T Cuts at: 2139 5073 2139 Size: 2934 5182 SfcI C'TryA_G Cuts at: 826 1045 2157 2166 2244 2471 2940 3266 3647 Size: 219 1112 9 78 227 469 326 381 Cuts at: 3647 3880 4411 4543 4773 4827 5360 5464 6599 Size: 233 531 132 230 54 533 104 1135 Cuts at: 6599 7280 7471 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1112 681 533 531 469 381 326 233 230 227 219 191 132 104 78 54 9 SfiI GGCCn_nnn'nGGCC Cuts at: 2180 2180 Size: 8116 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8116 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8116 SmaI CCC'GGG Cuts at: 1872 5443 1872 Size: 3571 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4985 5315 6220 7121 7383 820 Size: 197 926 3042 330 905 901 262 1553 Fragments arranged by size: 3042 1553 926 905 901 330 262 197 SnaBI TAC'GTA Cuts at: 493 3851 5907 493 Size: 3358 2056 2702 Fragments arranged by size: 3358 2702 2056 SpeI A'CTAG_T Cuts at: 152 152 Size: 8116 SphI G_CATG'C Cuts at: 1132 5474 1132 Size: 4342 3774 SspI AAT'ATT Cuts at: 5 52 2818 6040 5 Size: 47 2766 3222 2081 Fragments arranged by size: 3222 2766 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8116 StyI C'CwwG_G Cuts at: 513 1065 4518 4914 5555 513 Size: 552 3453 396 641 3074 Fragments arranged by size: 3453 3074 641 552 396 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3730 4691 4811 5045 6143 6160 6313 Size: 1255 2150 961 120 234 1098 17 153 Cuts at: 6313 6498 6943 325 Size: 185 445 1498 Fragments arranged by size: 2150 1498 1255 1098 961 445 234 185 153 120 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2501 4555 4853 Size: 274 80 33 51 1443 524 2054 298 Cuts at: 4853 6362 8074 96 Size: 1509 1712 138 Fragments arranged by size: 2054 1712 1509 1443 524 298 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2898 2930 3018 3050 5020 5052 5202 5234 8076 Size: 32 88 32 1970 32 150 32 2842 Cuts at: 8076 8108 2898 Size: 32 2906 Fragments arranged by size: 2906 2842 1970 150 88 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8116 XbaI T'CTAG_A Cuts at: 4435 5452 4435 Size: 1017 7099 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2147 2147 Size: 8116 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8116 XmaI C'CCGG_G Cuts at: 1870 5441 1870 Size: 3571 4545 XmnI GAAnn'nnTTC Cuts at: 2420 6245 2420 Size: 3825 4291 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5924 276 Size: 53 83 186 936 4390 2468 Fragments arranged by size: 4390 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII RsrII SacI SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI Bsu36I EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI SacII SanDI SapI SfoI SrfI SwaI