Restriction map for pFN21AE3461
 (Linear) (Six-base) MAPSORT of: KIEE3461.seq  Check: 1914  from: 1  to: 4059



KIEE3461.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 17:54  ..

AatII G_ACGT'C

Cuts at:      0    2466    4059 
   Size:       2466    1593
 
AccI GT'mk_AC

Cuts at:      0    1999    3413    4059 
   Size:       1999    1414     646

  Fragments arranged by size: 

                1999    1414     646
 
Acc65I G'GTAC_C

Cuts at:      0     289    4059 
   Size:        289    3770
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     371     506    2024    2089    2662    2748    3061    4009 
   Size:        371     135    1518      65     573      86     313     948

Cuts at:   4009    4059 
   Size:         50

  Fragments arranged by size: 

                1518     948     573     371     313     135      86      65
                  50
 
AflII C'TTAA_G

Cuts at:      0    1390    1513    4059 
   Size:       1390     123    2546

  Fragments arranged by size: 

                2546    1390     123
 
AflIII A'CryG_T

Cuts at:      0    3056    4059 
   Size:       3056    1003
 
AgeI A'CCGG_T

Cuts at:      0    1823    4059 
   Size:       1823    2236
 
AleI CACnn'nnGTG

Cuts at:      0     260    4059 
   Size:        260    3799
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     566    4059 
   Size:        566    3493
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1792    2365    3459    4059 
   Size:       1792     573    1094     600

  Fragments arranged by size: 

                1792    1094     600     573
 
ApaI G_GGCC'C

Cuts at:      0    3911    3971    4059 
   Size:       3911      60      88

  Fragments arranged by size: 

                3911      88      60
 
ApoI r'AATT_y

Cuts at:      0    1753    2138    3196    3236    3393    3469    4059 
   Size:       1753     385    1058      40     157      76     590

  Fragments arranged by size: 

                1753    1058     590     385     157      76      40
 
AseI AT'TA_AT

Cuts at:      0     980    1874    4059 
   Size:        980     894    2185

  Fragments arranged by size: 

                2185     980     894
 
AvaI C'yCGr_G

Cuts at:      0      29     878    1281    1484    3191    3215    4059 
   Size:         29     849     403     203    1707      24     844

  Fragments arranged by size: 

                1707     849     844     403     203      29      24
 
BamHI G'GATC_C

Cuts at:      0     120     246    4059 
   Size:        120     126    3813

  Fragments arranged by size: 

                3813     126     120
 
BanI G'GyrC_C

Cuts at:      0     268     289     325    1311    1744    4059 
   Size:        268      21      36     986     433    2315

  Fragments arranged by size: 

                2315     986     433     268      36      21
 
BanII G_rGCy'C

Cuts at:      0     301    2086    3340    3631    3848    3911    3971    4059 
   Size:        301    1785    1254     291     217      63      60      88

  Fragments arranged by size: 

                1785    1254     301     291     217      88      63      60
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1649    2846    4059 
   Size:       1649    1197    1213

  Fragments arranged by size: 

                1649    1213    1197
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1262    1296    4059 
   Size:       1262      34    2763

  Fragments arranged by size: 

                2763    1262      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     128     606    2889    3143    3443    4059 
   Size:        128     478    2283     254     300     616

  Fragments arranged by size: 

                2283     616     478     300     254     128
 
BclI T'GATC_A

Cuts at:      0    1917    2892    4059 
   Size:       1917     975    1167

  Fragments arranged by size: 

                1917    1167     975
 
BglI GCCn_nnn'nGGC

Cuts at:      0    3975    4059 
   Size:       3975      84
 
BlpI GC'TnA_GC

Cuts at:      0    1452    4059 
   Size:       1452    2607
 
Bme1580I G_kGCm'C

Cuts at:      0    3911    3971    3989    4059 
   Size:       3911      60      18      70

  Fragments arranged by size: 

                3911      70      60      18
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    3941    4059 
   Size:       3941     118
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     718    1286    2932    3753    3768    4059 
   Size:        718     568    1646     821      15     291

  Fragments arranged by size: 

                1646     821     718     568     291      15
 
Bpu10I CC'TnA_GC

Cuts at:      0    1235    2096    4059 
   Size:       1235     861    1963

  Fragments arranged by size: 

                1963    1235     861
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     523    1239    4059 
   Size:        523     716    2820

  Fragments arranged by size: 

                2820     716     523
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2821    3699    4059 
   Size:       2821     878     360

  Fragments arranged by size: 

                2821     878     360
 
BsaBI GATnn'nnATC

Cuts at:      0     251    2426    3248    4059 
   Size:        251    2175     822     811

  Fragments arranged by size: 

                2175     822     811     251
 
BsaHI Gr'CG_yC

Cuts at:      0    1646    2463    4059 
   Size:       1646     817    1596

  Fragments arranged by size: 

                1646    1596     817
 
BsaWI w'CCGG_w

Cuts at:      0     101    1823    4059 
   Size:        101    1722    2236

  Fragments arranged by size: 

                2236    1722     101
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    3766    3796    4059 
   Size:       3766      30     263

  Fragments arranged by size: 

                3766     263      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1895    3157    3631    4059 
   Size:       1895    1262     474     428

  Fragments arranged by size: 

                1895    1262     474     428
 
BseYI C'CCAG_C

Cuts at:      0     435     553    2185    2611    3632    3641    3910    4059 
   Size:        435     118    1632     426    1021       9     269     149

  Fragments arranged by size: 

                1632    1021     435     426     269     149     118       9
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1208    1225    3613    4059 
   Size:       1208      17    2388     446

  Fragments arranged by size: 

                2388    1208     446      17
 
BsiEI CG_ry'CG

Cuts at:      0    2728    3553    4059 
   Size:       2728     825     506

  Fragments arranged by size: 

                2728     825     506
 
BsiHKAI G_wGCw'C

Cuts at:      0     879    1215    3631    3848    4059 
   Size:        879     336    2416     217     211

  Fragments arranged by size: 

                2416     879     336     217     211
 
BsmI GAATG_Cn'

Cuts at:      0    1674    3041    4059 
   Size:       1674    1367    1018

  Fragments arranged by size: 

                1674    1367    1018
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     665    4059 
   Size:        665    3394
 
Bsp1286I G_dGCh'C

Cuts at:      0     301     879    1215    2086    3340    3631    3848    3911 
   Size:        301     578     336     871    1254     291     217      63

Cuts at:   3911    3971    3989    4059 
   Size:         60      18      70

  Fragments arranged by size: 

                1254     871     578     336     301     291     217      70
                  63      60      18
 
BspHI T'CATG_A

Cuts at:      0    3441    4059 
   Size:       3441     618
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     577    2593    4059 
   Size:        577    2016    1466

  Fragments arranged by size: 

                2016    1466     577
 
BsrBI CCG'CTC

Cuts at:      0    1300    3566    4059 
   Size:       1300    2266     493

  Fragments arranged by size: 

                2266    1300     493
 
BsrDI GCAATG_nn'

Cuts at:      0    2067    2760    4059 
   Size:       2067     693    1299

  Fragments arranged by size: 

                2067    1299     693
 
BsrFI r'CCGG_y

Cuts at:      0    1823    3552    4059 
   Size:       1823    1729     507

  Fragments arranged by size: 

                1823    1729     507
 
BssSI C'ACGA_G

Cuts at:      0    3341    4059 
   Size:       3341     718
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2750    3984    4059 
   Size:       2750    1234      75

  Fragments arranged by size: 

                2750    1234      75
 
BstBI TT'CG_AA

Cuts at:      0     996    4059 
   Size:        996    3063
 
BstEII G'GTnAC_C

Cuts at:      0      90    1819    4059 
   Size:         90    1729    2240

  Fragments arranged by size: 

                2240    1729      90
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     131    2514    4059 
   Size:        131    2383    1545

  Fragments arranged by size: 

                2383    1545     131
 
BstYI r'GATC_y

Cuts at:      0     120     246    1789    4059 
   Size:        120     126    1543    2270

  Fragments arranged by size: 

                2270    1543     126     120
 
BtgI C'CryG_G

Cuts at:      0     284     607    1973    2722    2728    2798    3311    3388 
   Size:        284     323    1366     749       6      70     513      77

Cuts at:   3388    3584    4059 
   Size:        196     475

  Fragments arranged by size: 

                1366     749     513     475     323     284     196      77
                  70       6
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      96     130    4059 
   Size:         96      34    3929

  Fragments arranged by size: 

                3929      96      34
 
BtsI GCAGTG_nn'

Cuts at:      0     236     548    1350    4059 
   Size:        236     312     802    2709

  Fragments arranged by size: 

                2709     802     312     236
 
DraIII CAC_nnn'GTG

Cuts at:      0    1203    3579    4059 
   Size:       1203    2376     480

  Fragments arranged by size: 

                2376    1203     480
 
EaeI y'GGCC_r

Cuts at:      0      54    1493    1506    2725    3538    3550    4059 
   Size:         54    1439      13    1219     813      12     509

  Fragments arranged by size: 

                1439    1219     813     509      54      13      12
 
EagI C'GGCC_G

Cuts at:      0    2725    3550    4059 
   Size:       2725     825     509

  Fragments arranged by size: 

                2725     825     509
 
EarI CTCTTCn'nnn_

Cuts at:      0     416     482     725    2328    3134    4059 
   Size:        416      66     243    1603     806     925

  Fragments arranged by size: 

                1603     925     806     416     243      66
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3528    3882    4059 
   Size:       3528     354     177

  Fragments arranged by size: 

                3528     354     177
 
EcoICRI GAG'CTC

Cuts at:      0    3629    3846    4059 
   Size:       3629     217     213

  Fragments arranged by size: 

                3629     217     213
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     371     506     718    1286    2024    2089    2662    2748 
   Size:        371     135     212     568     738      65     573      86

Cuts at:   2748    2932    3061    3753    3768    4009    4059 
   Size:        184     129     692      15     241      50

  Fragments arranged by size: 

                 738     692     573     568     371     241     212     184
                 135     129      86      65      50      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     573    3731    3853    4037    4059 
   Size:        573    3158     122     184      22

  Fragments arranged by size: 

                3158     573     184     122      22
 
EcoO109I rG'GnC_Cy

Cuts at:      0     844    2626    3907    3942    3967    3968    4046    4059 
   Size:        844    1782    1281      35      25       1      78      13

  Fragments arranged by size: 

                1782    1281     844      78      35      25      13       1
            
 
EcoRI G'AATT_C

Cuts at:      0    3393    4059 
   Size:       3393     666
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1792    3987    4059 
   Size:       1792    2195      72

  Fragments arranged by size: 

                2195    1792      72
 
HaeII r_GCGC'y

Cuts at:      0    3648    4059 
   Size:       3648     411
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     657     689    1233    1237    1265    1269    1406    1438 
   Size:        657      32     544       4      28       4     137      32

Cuts at:   1438    1774    1806    2009    2041    2056    2088    2305    2337 
   Size:        336      32     203      32      15      32     217      32

Cuts at:   2337    2759    2791    2863    2895    3441    3473    4059 
   Size:        422      32      72      32     546      32     586

  Fragments arranged by size: 

                 657     586     546     544     422     336     217     203
                 137      72      32      32      32      32      32      32
                  32      32      32      28      15       4       4
 
HincII GTy'rAC

Cuts at:      0     521    4059 
   Size:        521    3538
 
KpnI G_GTAC'C

Cuts at:      0     293    4059 
   Size:        293    3766
 
MfeI C'AATT_G

Cuts at:      0    2512    4059 
   Size:       2512    1547
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     738    2099    2639    4059 
   Size:        738    1361     540    1420

  Fragments arranged by size: 

                1420    1361     738     540
 
MslI CAynn'nnrTG

Cuts at:      0     161     260    1603    2339    4059 
   Size:        161      99    1343     736    1720

  Fragments arranged by size: 

                1720    1343     736     161      99
 
MspA1I CmG'CkG

Cuts at:      0     529     609    2229    2730    2800    3390    3603    3609 
   Size:        529      80    1620     501      70     590     213       6

Cuts at:   3609    4059 
   Size:        450

  Fragments arranged by size: 

                1620     590     529     501     450     213      80      70
                   6
 
NaeI GCC'GGC

Cuts at:      0    3554    4059 
   Size:       3554     505
 
NcoI C'CATG_G

Cuts at:      0    1973    3311    4059 
   Size:       1973    1338     748

  Fragments arranged by size: 

                1973    1338     748
 
NdeI CA'TA_TG

Cuts at:      0    1025    3353    4059 
   Size:       1025    2328     706

  Fragments arranged by size: 

                2328    1025     706
 
NgoMIV G'CCGG_C

Cuts at:      0    3552    4059 
   Size:       3552     507
 
NspI r_CATG'y

Cuts at:      0     766    3060    4059 
   Size:        766    2294     999

  Fragments arranged by size: 

                2294     999     766
 
PasI CC'CwG_GG

Cuts at:      0     894    2634    3727    4059 
   Size:        894    1740    1093     332

  Fragments arranged by size: 

                1740    1093     894     332
 
PciI A'CATG_T

Cuts at:      0    3056    4059 
   Size:       3056    1003
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     515    1058    2910    4059 
   Size:        515     543    1852    1149

  Fragments arranged by size: 

                1852    1149     543     515
 
PfoI T'CCnGG_A

Cuts at:      0    3784    3938    4059 
   Size:       3784     154     121

  Fragments arranged by size: 

                3784     154     121
 
PpuMI rG'GwC_Cy

Cuts at:      0    2626    3942    4059 
   Size:       2626    1316     117

  Fragments arranged by size: 

                2626    1316     117
 
PsiI TTA'TAA

Cuts at:      0    1352    4059 
   Size:       1352    2707
 
PspOMI G'GGCC_C

Cuts at:      0    3907    3967    4059 
   Size:       3907      60      92

  Fragments arranged by size: 

                3907      92      60
 
PspXI vC'TCGA_Gb

Cuts at:      0     878    4059 
   Size:        878    3181
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    3748    3780    4059 
   Size:       3748      32     279

  Fragments arranged by size: 

                3748     279      32
 
PstI C_TGCA'G

Cuts at:      0    1080    1797    2672    4059 
   Size:       1080     717     875    1387

  Fragments arranged by size: 

                1387    1080     875     717
 
PvuII CAG'CTG

Cuts at:      0     529    2229    4059 
   Size:        529    1700    1830

  Fragments arranged by size: 

                1830    1700     529
 
SacI G_AGCT'C

Cuts at:      0    3631    3848    4059 
   Size:       3631     217     211

  Fragments arranged by size: 

                3631     217     211
 
SacII CC_GC'GG

Cuts at:      0     610    2731    2801    3391    4059 
   Size:        610    2121      70     590     668

  Fragments arranged by size: 

                2121     668     610     590      70
 
SapI GCTCTTCn'nnn_

Cuts at:      0     482    4059 
   Size:        482    3577
 
SfcI C'TryA_G

Cuts at:      0     594    1076    1793    2154    2237    2668    3410    3519 
   Size:        594     482     717     361      83     431     742     109

Cuts at:   3519    4059 
   Size:        540

  Fragments arranged by size: 

                 742     717     594     540     482     431     361     109
                  83
 
SmaI CCC'GGG

Cuts at:      0      31    1486    4059 
   Size:         31    1455    2573

  Fragments arranged by size: 

                2573    1455      31
 
SmlI C'TyrA_G

Cuts at:      0     538     878    1254    1390    1513    3191    3215    4059 
   Size:        538     340     376     136     123    1678      24     844

  Fragments arranged by size: 

                1678     844     538     376     340     136     123      24
            
 
SpeI A'CTAG_T

Cuts at:      0     616    4059 
   Size:        616    3443
 
StuI AGG'CCT

Cuts at:      0    1799    4059 
   Size:       1799    2260
 
StyI C'CwwG_G

Cuts at:      0     116    1657    1800    1973    2378    2831    3311    3777 
   Size:        116    1541     143     173     405     453     480     466

Cuts at:   3777    3962    4059 
   Size:        185      97

  Fragments arranged by size: 

                1541     480     466     453     405     185     173     143
                 116      97
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     692    1065    1670    3681    4059 
   Size:        692     373     605    2011     378

  Fragments arranged by size: 

                2011     692     605     378     373
 
TatI w'GTAC_w

Cuts at:      0    3650    4059 
   Size:       3650     409
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     374     406    3565    3597    4059 
   Size:        374      32    3159      32     462

  Fragments arranged by size: 

                3159     462     374      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2263    4059 
   Size:       2263    1796
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     678     798    2619    3828    4059 
   Size:        678     120    1821    1209     231

  Fragments arranged by size: 

                1821    1209     678     231     120
 
XhoI C'TCGA_G

Cuts at:      0     878    3191    3215    4059 
   Size:        878    2313      24     844

  Fragments arranged by size: 

                2313     878     844      24
 
XmaI C'CCGG_G

Cuts at:      0      29    1484    4059 
   Size:         29    1455    2575

  Fragments arranged by size: 

                2575    1455      29
 
XmnI GAAnn'nnTTC

Cuts at:      0      67    2139    4059 
   Size:         67    2072    1920

  Fragments arranged by size: 

                2072    1920      67
 
ZraI GAC'GTC

Cuts at:      0    2464    4059 
   Size:       2464    1595
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AcuI    AflII   AflIII     AgeI     AleI
     AlfI    AlwNI     ApaI     ApoI     AseI     AvaI    BamHI     BanI
    BanII     BbsI     BcgI    BciVI     BclI     BglI     BlpI Bme1580I
     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI   DraIII
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI    HaeII    Hin4I   HincII     KpnI     MfeI     MmeI
     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NspI     PasI
     PciI    PflMI     PfoI    PpuMI     PsiI   PspOMI    PspXI     PsrI
     PstI    PvuII     SacI    SacII     SapI     SfcI     SmaI     SmlI
     SpeI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AclI     AfeI     AhdI     AloI    ApaLI     AscI    AsiSI
    AvrII     BaeI     BbeI    BbvCI    BfrBI    BglII    BmgBI     BmtI
     BplI    BsaAI    BsiWI    BspEI    BsrGI   BssHII  BstZ17I   Bsu36I
     ClaI    CspCI     DraI     DrdI    EcoRV     FseI     FspI    FspAI
  HindIII     HpaI     KasI     MluI     MscI     NarI     NheI     NotI
     NruI     NsiI     PacI     PmeI     PmlI     PpiI    PshAI     PvuI
    RsrII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SphI     SrfI     SspI     SwaI     XbaI
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