(Linear) (Six-base) MAPSORT of: KIEE3461.seq Check: 1914 from: 1 to: 4059 KIEE3461.orf With 182 enzymes: SgfI * July 3, 2012 17:54 .. AatII G_ACGT'C Cuts at: 0 2466 4059 Size: 2466 1593 AccI GT'mk_AC Cuts at: 0 1999 3413 4059 Size: 1999 1414 646 Fragments arranged by size: 1999 1414 646 Acc65I G'GTAC_C Cuts at: 0 289 4059 Size: 289 3770 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 371 506 2024 2089 2662 2748 3061 4009 Size: 371 135 1518 65 573 86 313 948 Cuts at: 4009 4059 Size: 50 Fragments arranged by size: 1518 948 573 371 313 135 86 65 50 AflII C'TTAA_G Cuts at: 0 1390 1513 4059 Size: 1390 123 2546 Fragments arranged by size: 2546 1390 123 AflIII A'CryG_T Cuts at: 0 3056 4059 Size: 3056 1003 AgeI A'CCGG_T Cuts at: 0 1823 4059 Size: 1823 2236 AleI CACnn'nnGTG Cuts at: 0 260 4059 Size: 260 3799 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 566 4059 Size: 566 3493 AlwNI CAG_nnn'CTG Cuts at: 0 1792 2365 3459 4059 Size: 1792 573 1094 600 Fragments arranged by size: 1792 1094 600 573 ApaI G_GGCC'C Cuts at: 0 3911 3971 4059 Size: 3911 60 88 Fragments arranged by size: 3911 88 60 ApoI r'AATT_y Cuts at: 0 1753 2138 3196 3236 3393 3469 4059 Size: 1753 385 1058 40 157 76 590 Fragments arranged by size: 1753 1058 590 385 157 76 40 AseI AT'TA_AT Cuts at: 0 980 1874 4059 Size: 980 894 2185 Fragments arranged by size: 2185 980 894 AvaI C'yCGr_G Cuts at: 0 29 878 1281 1484 3191 3215 4059 Size: 29 849 403 203 1707 24 844 Fragments arranged by size: 1707 849 844 403 203 29 24 BamHI G'GATC_C Cuts at: 0 120 246 4059 Size: 120 126 3813 Fragments arranged by size: 3813 126 120 BanI G'GyrC_C Cuts at: 0 268 289 325 1311 1744 4059 Size: 268 21 36 986 433 2315 Fragments arranged by size: 2315 986 433 268 36 21 BanII G_rGCy'C Cuts at: 0 301 2086 3340 3631 3848 3911 3971 4059 Size: 301 1785 1254 291 217 63 60 88 Fragments arranged by size: 1785 1254 301 291 217 88 63 60 BbsI GAAGACnn'nnnn_ Cuts at: 0 1649 2846 4059 Size: 1649 1197 1213 Fragments arranged by size: 1649 1213 1197 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1262 1296 4059 Size: 1262 34 2763 Fragments arranged by size: 2763 1262 34 BciVI GTATCCnnnnn_n' Cuts at: 0 128 606 2889 3143 3443 4059 Size: 128 478 2283 254 300 616 Fragments arranged by size: 2283 616 478 300 254 128 BclI T'GATC_A Cuts at: 0 1917 2892 4059 Size: 1917 975 1167 Fragments arranged by size: 1917 1167 975 BglI GCCn_nnn'nGGC Cuts at: 0 3975 4059 Size: 3975 84 BlpI GC'TnA_GC Cuts at: 0 1452 4059 Size: 1452 2607 Bme1580I G_kGCm'C Cuts at: 0 3911 3971 3989 4059 Size: 3911 60 18 70 Fragments arranged by size: 3911 70 60 18 BmrI ACTGGGnnnn_n' Cuts at: 0 3941 4059 Size: 3941 118 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 718 1286 2932 3753 3768 4059 Size: 718 568 1646 821 15 291 Fragments arranged by size: 1646 821 718 568 291 15 Bpu10I CC'TnA_GC Cuts at: 0 1235 2096 4059 Size: 1235 861 1963 Fragments arranged by size: 1963 1235 861 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 523 1239 4059 Size: 523 716 2820 Fragments arranged by size: 2820 716 523 BsaI GGTCTCn'nnnn_ Cuts at: 0 2821 3699 4059 Size: 2821 878 360 Fragments arranged by size: 2821 878 360 BsaBI GATnn'nnATC Cuts at: 0 251 2426 3248 4059 Size: 251 2175 822 811 Fragments arranged by size: 2175 822 811 251 BsaHI Gr'CG_yC Cuts at: 0 1646 2463 4059 Size: 1646 817 1596 Fragments arranged by size: 1646 1596 817 BsaWI w'CCGG_w Cuts at: 0 101 1823 4059 Size: 101 1722 2236 Fragments arranged by size: 2236 1722 101 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 3766 3796 4059 Size: 3766 30 263 Fragments arranged by size: 3766 263 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1895 3157 3631 4059 Size: 1895 1262 474 428 Fragments arranged by size: 1895 1262 474 428 BseYI C'CCAG_C Cuts at: 0 435 553 2185 2611 3632 3641 3910 4059 Size: 435 118 1632 426 1021 9 269 149 Fragments arranged by size: 1632 1021 435 426 269 149 118 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1208 1225 3613 4059 Size: 1208 17 2388 446 Fragments arranged by size: 2388 1208 446 17 BsiEI CG_ry'CG Cuts at: 0 2728 3553 4059 Size: 2728 825 506 Fragments arranged by size: 2728 825 506 BsiHKAI G_wGCw'C Cuts at: 0 879 1215 3631 3848 4059 Size: 879 336 2416 217 211 Fragments arranged by size: 2416 879 336 217 211 BsmI GAATG_Cn' Cuts at: 0 1674 3041 4059 Size: 1674 1367 1018 Fragments arranged by size: 1674 1367 1018 BsmBI CGTCTCn'nnnn_ Cuts at: 0 665 4059 Size: 665 3394 Bsp1286I G_dGCh'C Cuts at: 0 301 879 1215 2086 3340 3631 3848 3911 Size: 301 578 336 871 1254 291 217 63 Cuts at: 3911 3971 3989 4059 Size: 60 18 70 Fragments arranged by size: 1254 871 578 336 301 291 217 70 63 60 18 BspHI T'CATG_A Cuts at: 0 3441 4059 Size: 3441 618 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 577 2593 4059 Size: 577 2016 1466 Fragments arranged by size: 2016 1466 577 BsrBI CCG'CTC Cuts at: 0 1300 3566 4059 Size: 1300 2266 493 Fragments arranged by size: 2266 1300 493 BsrDI GCAATG_nn' Cuts at: 0 2067 2760 4059 Size: 2067 693 1299 Fragments arranged by size: 2067 1299 693 BsrFI r'CCGG_y Cuts at: 0 1823 3552 4059 Size: 1823 1729 507 Fragments arranged by size: 1823 1729 507 BssSI C'ACGA_G Cuts at: 0 3341 4059 Size: 3341 718 BstAPI GCAn_nnn'nTGC Cuts at: 0 2750 3984 4059 Size: 2750 1234 75 Fragments arranged by size: 2750 1234 75 BstBI TT'CG_AA Cuts at: 0 996 4059 Size: 996 3063 BstEII G'GTnAC_C Cuts at: 0 90 1819 4059 Size: 90 1729 2240 Fragments arranged by size: 2240 1729 90 BstXI CCAn_nnnn'nTGG Cuts at: 0 131 2514 4059 Size: 131 2383 1545 Fragments arranged by size: 2383 1545 131 BstYI r'GATC_y Cuts at: 0 120 246 1789 4059 Size: 120 126 1543 2270 Fragments arranged by size: 2270 1543 126 120 BtgI C'CryG_G Cuts at: 0 284 607 1973 2722 2728 2798 3311 3388 Size: 284 323 1366 749 6 70 513 77 Cuts at: 3388 3584 4059 Size: 196 475 Fragments arranged by size: 1366 749 513 475 323 284 196 77 70 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 96 130 4059 Size: 96 34 3929 Fragments arranged by size: 3929 96 34 BtsI GCAGTG_nn' Cuts at: 0 236 548 1350 4059 Size: 236 312 802 2709 Fragments arranged by size: 2709 802 312 236 DraIII CAC_nnn'GTG Cuts at: 0 1203 3579 4059 Size: 1203 2376 480 Fragments arranged by size: 2376 1203 480 EaeI y'GGCC_r Cuts at: 0 54 1493 1506 2725 3538 3550 4059 Size: 54 1439 13 1219 813 12 509 Fragments arranged by size: 1439 1219 813 509 54 13 12 EagI C'GGCC_G Cuts at: 0 2725 3550 4059 Size: 2725 825 509 Fragments arranged by size: 2725 825 509 EarI CTCTTCn'nnn_ Cuts at: 0 416 482 725 2328 3134 4059 Size: 416 66 243 1603 806 925 Fragments arranged by size: 1603 925 806 416 243 66 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 3528 3882 4059 Size: 3528 354 177 Fragments arranged by size: 3528 354 177 EcoICRI GAG'CTC Cuts at: 0 3629 3846 4059 Size: 3629 217 213 Fragments arranged by size: 3629 217 213 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 371 506 718 1286 2024 2089 2662 2748 Size: 371 135 212 568 738 65 573 86 Cuts at: 2748 2932 3061 3753 3768 4009 4059 Size: 184 129 692 15 241 50 Fragments arranged by size: 738 692 573 568 371 241 212 184 135 129 86 65 50 15 EcoNI CCTnn'n_nnAGG Cuts at: 0 573 3731 3853 4037 4059 Size: 573 3158 122 184 22 Fragments arranged by size: 3158 573 184 122 22 EcoO109I rG'GnC_Cy Cuts at: 0 844 2626 3907 3942 3967 3968 4046 4059 Size: 844 1782 1281 35 25 1 78 13 Fragments arranged by size: 1782 1281 844 78 35 25 13 1 EcoRI G'AATT_C Cuts at: 0 3393 4059 Size: 3393 666 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1792 3987 4059 Size: 1792 2195 72 Fragments arranged by size: 2195 1792 72 HaeII r_GCGC'y Cuts at: 0 3648 4059 Size: 3648 411 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 657 689 1233 1237 1265 1269 1406 1438 Size: 657 32 544 4 28 4 137 32 Cuts at: 1438 1774 1806 2009 2041 2056 2088 2305 2337 Size: 336 32 203 32 15 32 217 32 Cuts at: 2337 2759 2791 2863 2895 3441 3473 4059 Size: 422 32 72 32 546 32 586 Fragments arranged by size: 657 586 546 544 422 336 217 203 137 72 32 32 32 32 32 32 32 32 32 28 15 4 4 HincII GTy'rAC Cuts at: 0 521 4059 Size: 521 3538 KpnI G_GTAC'C Cuts at: 0 293 4059 Size: 293 3766 MfeI C'AATT_G Cuts at: 0 2512 4059 Size: 2512 1547 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 738 2099 2639 4059 Size: 738 1361 540 1420 Fragments arranged by size: 1420 1361 738 540 MslI CAynn'nnrTG Cuts at: 0 161 260 1603 2339 4059 Size: 161 99 1343 736 1720 Fragments arranged by size: 1720 1343 736 161 99 MspA1I CmG'CkG Cuts at: 0 529 609 2229 2730 2800 3390 3603 3609 Size: 529 80 1620 501 70 590 213 6 Cuts at: 3609 4059 Size: 450 Fragments arranged by size: 1620 590 529 501 450 213 80 70 6 NaeI GCC'GGC Cuts at: 0 3554 4059 Size: 3554 505 NcoI C'CATG_G Cuts at: 0 1973 3311 4059 Size: 1973 1338 748 Fragments arranged by size: 1973 1338 748 NdeI CA'TA_TG Cuts at: 0 1025 3353 4059 Size: 1025 2328 706 Fragments arranged by size: 2328 1025 706 NgoMIV G'CCGG_C Cuts at: 0 3552 4059 Size: 3552 507 NspI r_CATG'y Cuts at: 0 766 3060 4059 Size: 766 2294 999 Fragments arranged by size: 2294 999 766 PasI CC'CwG_GG Cuts at: 0 894 2634 3727 4059 Size: 894 1740 1093 332 Fragments arranged by size: 1740 1093 894 332 PciI A'CATG_T Cuts at: 0 3056 4059 Size: 3056 1003 PflMI CCAn_nnn'nTGG Cuts at: 0 515 1058 2910 4059 Size: 515 543 1852 1149 Fragments arranged by size: 1852 1149 543 515 PfoI T'CCnGG_A Cuts at: 0 3784 3938 4059 Size: 3784 154 121 Fragments arranged by size: 3784 154 121 PpuMI rG'GwC_Cy Cuts at: 0 2626 3942 4059 Size: 2626 1316 117 Fragments arranged by size: 2626 1316 117 PsiI TTA'TAA Cuts at: 0 1352 4059 Size: 1352 2707 PspOMI G'GGCC_C Cuts at: 0 3907 3967 4059 Size: 3907 60 92 Fragments arranged by size: 3907 92 60 PspXI vC'TCGA_Gb Cuts at: 0 878 4059 Size: 878 3181 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 3748 3780 4059 Size: 3748 32 279 Fragments arranged by size: 3748 279 32 PstI C_TGCA'G Cuts at: 0 1080 1797 2672 4059 Size: 1080 717 875 1387 Fragments arranged by size: 1387 1080 875 717 PvuII CAG'CTG Cuts at: 0 529 2229 4059 Size: 529 1700 1830 Fragments arranged by size: 1830 1700 529 SacI G_AGCT'C Cuts at: 0 3631 3848 4059 Size: 3631 217 211 Fragments arranged by size: 3631 217 211 SacII CC_GC'GG Cuts at: 0 610 2731 2801 3391 4059 Size: 610 2121 70 590 668 Fragments arranged by size: 2121 668 610 590 70 SapI GCTCTTCn'nnn_ Cuts at: 0 482 4059 Size: 482 3577 SfcI C'TryA_G Cuts at: 0 594 1076 1793 2154 2237 2668 3410 3519 Size: 594 482 717 361 83 431 742 109 Cuts at: 3519 4059 Size: 540 Fragments arranged by size: 742 717 594 540 482 431 361 109 83 SmaI CCC'GGG Cuts at: 0 31 1486 4059 Size: 31 1455 2573 Fragments arranged by size: 2573 1455 31 SmlI C'TyrA_G Cuts at: 0 538 878 1254 1390 1513 3191 3215 4059 Size: 538 340 376 136 123 1678 24 844 Fragments arranged by size: 1678 844 538 376 340 136 123 24 SpeI A'CTAG_T Cuts at: 0 616 4059 Size: 616 3443 StuI AGG'CCT Cuts at: 0 1799 4059 Size: 1799 2260 StyI C'CwwG_G Cuts at: 0 116 1657 1800 1973 2378 2831 3311 3777 Size: 116 1541 143 173 405 453 480 466 Cuts at: 3777 3962 4059 Size: 185 97 Fragments arranged by size: 1541 480 466 453 405 185 173 143 116 97 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 692 1065 1670 3681 4059 Size: 692 373 605 2011 378 Fragments arranged by size: 2011 692 605 378 373 TatI w'GTAC_w Cuts at: 0 3650 4059 Size: 3650 409 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 374 406 3565 3597 4059 Size: 374 32 3159 32 462 Fragments arranged by size: 3159 462 374 32 32 Tth111I GACn'n_nGTC Cuts at: 0 2263 4059 Size: 2263 1796 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 678 798 2619 3828 4059 Size: 678 120 1821 1209 231 Fragments arranged by size: 1821 1209 678 231 120 XhoI C'TCGA_G Cuts at: 0 878 3191 3215 4059 Size: 878 2313 24 844 Fragments arranged by size: 2313 878 844 24 XmaI C'CCGG_G Cuts at: 0 29 1484 4059 Size: 29 1455 2575 Fragments arranged by size: 2575 1455 29 XmnI GAAnn'nnTTC Cuts at: 0 67 2139 4059 Size: 67 2072 1920 Fragments arranged by size: 2072 1920 67 ZraI GAC'GTC Cuts at: 0 2464 4059 Size: 2464 1595 Enzymes that do cut: AatII AccI Acc65I AcuI AflII AflIII AgeI AleI AlfI AlwNI ApaI ApoI AseI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII KpnI MfeI MmeI MslI MspA1I NaeI NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI PpuMI PsiI PspOMI PspXI PsrI PstI PvuII SacI SacII SapI SfcI SmaI SmlI SpeI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AclI AfeI AhdI AloI ApaLI AscI AsiSI AvrII BaeI BbeI BbvCI BfrBI BglII BmgBI BmtI BplI BsaAI BsiWI BspEI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraI DrdI EcoRV FseI FspI FspAI HindIII HpaI KasI MluI MscI NarI NheI NotI NruI NsiI PacI PmeI PmlI PpiI PshAI PvuI RsrII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SphI SrfI SspI SwaI XbaI