Restriction map for
pFN21AE2110
(Linear) (Six-base) MAPSORT of: KIEE2110.seq Check: 8801 from: 1 to: 2718
KIEE2110.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 456 978 2462 2718
Size: 456 522 1484 256
Fragments arranged by size:
1484 522 456 256
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2178 2718
Size: 2178 540
AlwNI CAG_nnn'CTG
Cuts at: 0 556 1801 2718
Size: 556 1245 917
Fragments arranged by size:
1245 917 556
ApaLI G'TGCA_C
Cuts at: 0 1200 2718
Size: 1200 1518
ApoI r'AATT_y
Cuts at: 0 360 429 562 718 847 2259 2680 2718
Size: 360 69 133 156 129 1412 421 38
Fragments arranged by size:
1412 421 360 156 133 129 69 38
AvaI C'yCGr_G
Cuts at: 0 57 2290 2458 2718
Size: 57 2233 168 260
Fragments arranged by size:
2233 260 168 57
AvrII C'CTAG_G
Cuts at: 0 439 1903 2718
Size: 439 1464 815
Fragments arranged by size:
1464 815 439
BanI G'GyrC_C
Cuts at: 0 1423 1485 2220 2718
Size: 1423 62 735 498
Fragments arranged by size:
1423 735 498 62
BanII G_rGCy'C
Cuts at: 0 25 58 83 1126 1184 1350 1889 2718
Size: 25 33 25 1043 58 166 539 829
Fragments arranged by size:
1043 829 539 166 58 33 25 25
BbeI G_GCGC'C
Cuts at: 0 2224 2718
Size: 2224 494
BbvCI CC'TCA_GC
Cuts at: 0 127 2564 2718
Size: 127 2437 154
Fragments arranged by size:
2437 154 127
BciVI GTATCCnnnnn_n'
Cuts at: 0 1923 2127 2718
Size: 1923 204 591
Fragments arranged by size:
1923 591 204
BglI GCCn_nnn'nGGC
Cuts at: 0 2124 2353 2718
Size: 2124 229 365
Fragments arranged by size:
2124 365 229
BglII A'GATC_T
Cuts at: 0 1620 2718
Size: 1620 1098
BlpI GC'TnA_GC
Cuts at: 0 883 2718
Size: 883 1835
Bme1580I G_kGCm'C
Cuts at: 0 1204 2718
Size: 1204 1514
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 908 1140 2718
Size: 908 232 1578
Fragments arranged by size:
1578 908 232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 826 928 1625 1704 2556 2718
Size: 676 150 102 697 79 852 162
Fragments arranged by size:
852 697 676 162 150 102 79
Bpu10I CC'TnA_GC
Cuts at: 0 127 763 2564 2718
Size: 127 636 1801 154
Fragments arranged by size:
1801 636 154 127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1515 2718
Size: 1515 1203
BsaAI yAC'GTr
Cuts at: 0 2189 2718
Size: 2189 529
BsaBI GATnn'nnATC
Cuts at: 0 2420 2718
Size: 2420 298
BsaHI Gr'CG_yC
Cuts at: 0 92 1686 2221 2718
Size: 92 1594 535 497
Fragments arranged by size:
1594 535 497 92
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1702 1732 2718
Size: 1702 30 986
Fragments arranged by size:
1702 986 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 439 2308 2382 2718
Size: 439 1869 74 336
Fragments arranged by size:
1869 439 336 74
BsiEI CG_ry'CG
Cuts at: 0 1441 2348 2718
Size: 1441 907 370
Fragments arranged by size:
1441 907 370
BsiHKAI G_wGCw'C
Cuts at: 0 25 1204 1350 2718
Size: 25 1179 146 1368
Fragments arranged by size:
1368 1179 146 25
Bsp1286I G_dGCh'C
Cuts at: 0 25 58 83 1126 1184 1204 1350 1889
Size: 25 33 25 1043 58 20 146 539
Cuts at: 1889 2718
Size: 829
Fragments arranged by size:
1043 829 539 146 58 33 25 25
20
BspHI T'CATG_A
Cuts at: 0 83 1403 2591 2718
Size: 83 1320 1188 127
Fragments arranged by size:
1320 1188 127 83
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 266 700 2718
Size: 266 434 2018
Fragments arranged by size:
2018 434 266
BsrDI GCAATG_nn'
Cuts at: 0 702 1594 1867 2077 2204 2636 2718
Size: 702 892 273 210 127 432 82
Fragments arranged by size:
892 702 432 273 210 127 82
BsrFI r'CCGG_y
Cuts at: 0 4 1646 1847 2343 2718
Size: 4 1642 201 496 375
Fragments arranged by size:
1642 496 375 201 4
BsrGI T'GTAC_A
Cuts at: 0 1699 2718
Size: 1699 1019
BssSI C'ACGA_G
Cuts at: 0 2150 2718
Size: 2150 568
BstAPI GCAn_nnn'nTGC
Cuts at: 0 556 2275 2718
Size: 556 1719 443
Fragments arranged by size:
1719 556 443
BstYI r'GATC_y
Cuts at: 0 1293 1620 2718
Size: 1293 327 1098
Fragments arranged by size:
1293 1098 327
BtgI C'CryG_G
Cuts at: 0 662 1457 2482 2718
Size: 662 795 1025 236
Fragments arranged by size:
1025 795 662 236
EaeI y'GGCC_r
Cuts at: 0 2 1438 1648 1777 2264 2345 2660 2718
Size: 2 1436 210 129 487 81 315 58
Fragments arranged by size:
1436 487 315 210 129 81 58 2
EagI C'GGCC_G
Cuts at: 0 1438 2345 2718
Size: 1438 907 373
Fragments arranged by size:
1438 907 373
EarI CTCTTCn'nnn_
Cuts at: 0 13 400 601 976 1405 1827 2045 2078
Size: 13 387 201 375 429 422 218 33
Cuts at: 2078 2115 2718
Size: 37 603
Fragments arranged by size:
603 429 422 387 375 218 201 37
33 13
EcoICRI GAG'CTC
Cuts at: 0 23 1348 2718
Size: 23 1325 1370
Fragments arranged by size:
1370 1325 23
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 456 676 826 928 978 1625 1704 2462
Size: 456 220 150 102 50 647 79 758
Cuts at: 2462 2556 2718
Size: 94 162
Fragments arranged by size:
758 647 456 220 162 150 102 94
79 50
EcoNI CCTnn'n_nnAGG
Cuts at: 0 397 2112 2718
Size: 397 1715 606
Fragments arranged by size:
1715 606 397
EcoRI G'AATT_C
Cuts at: 0 429 2718
Size: 429 2289
EcoRV GAT'ATC
Cuts at: 0 2376 2718
Size: 2376 342
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 111 1492 2718
Size: 111 1381 1226
Fragments arranged by size:
1381 1226 111
HaeII r_GCGC'y
Cuts at: 0 229 2224 2718
Size: 229 1995 494
Fragments arranged by size:
1995 494 229
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 939 971 1702 1734 2718
Size: 939 32 731 32 984
Fragments arranged by size:
984 939 731 32 32
HindIII A'AGCT_T
Cuts at: 0 575 1469 2627 2718
Size: 575 894 1158 91
Fragments arranged by size:
1158 894 575 91
KasI G'GCGC_C
Cuts at: 0 2220 2718
Size: 2220 498
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1158 1641 2718
Size: 1158 483 1077
Fragments arranged by size:
1158 1077 483
MscI TGG'CCA
Cuts at: 0 2662 2718
Size: 2662 56
MslI CAynn'nnrTG
Cuts at: 0 1942 2154 2656 2718
Size: 1942 212 502 62
Fragments arranged by size:
1942 502 212 62
MspA1I CmG'CkG
Cuts at: 0 44 2484 2516 2718
Size: 44 2440 32 202
Fragments arranged by size:
2440 202 44 32
NaeI GCC'GGC
Cuts at: 0 6 2345 2718
Size: 6 2339 373
Fragments arranged by size:
2339 373 6
NarI GG'CG_CC
Cuts at: 0 2221 2718
Size: 2221 497
NcoI C'CATG_G
Cuts at: 0 662 1457 2718
Size: 662 795 1261
Fragments arranged by size:
1261 795 662
NgoMIV G'CCGG_C
Cuts at: 0 4 2343 2718
Size: 4 2339 375
Fragments arranged by size:
2339 375 4
NspI r_CATG'y
Cuts at: 0 483 2159 2718
Size: 483 1676 559
Fragments arranged by size:
1676 559 483
PflMI CCAn_nnn'nTGG
Cuts at: 0 483 2718
Size: 483 2235
PfoI T'CCnGG_A
Cuts at: 0 2675 2718
Size: 2675 43
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1508 1540 2718
Size: 1508 32 1178
Fragments arranged by size:
1508 1178 32
PshAI GACnn'nnGTC
Cuts at: 0 595 2718
Size: 595 2123
PvuII CAG'CTG
Cuts at: 0 2516 2718
Size: 2516 202
SacI G_AGCT'C
Cuts at: 0 25 1350 2718
Size: 25 1325 1368
Fragments arranged by size:
1368 1325 25
SacII CC_GC'GG
Cuts at: 0 2485 2718
Size: 2485 233
SapI GCTCTTCn'nnn_
Cuts at: 0 13 601 2718
Size: 13 588 2117
Fragments arranged by size:
2117 588 13
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2124 2353 2718
Size: 2124 229 365
Fragments arranged by size:
2124 365 229
SfoI GGC'GCC
Cuts at: 0 2222 2718
Size: 2222 496
SmlI C'TyrA_G
Cuts at: 0 1530 2718
Size: 1530 1188
SnaBI TAC'GTA
Cuts at: 0 2189 2718
Size: 2189 529
SspI AAT'ATT
Cuts at: 0 2208 2718
Size: 2208 510
StuI AGG'CCT
Cuts at: 0 2118 2466 2718
Size: 2118 348 252
Fragments arranged by size:
2118 348 252
StyI C'CwwG_G
Cuts at: 0 439 601 662 1427 1457 1755 1903 2718
Size: 439 162 61 765 30 298 148 815
Fragments arranged by size:
815 765 439 298 162 148 61 30
TatI w'GTAC_w
Cuts at: 0 889 1699 2096 2718
Size: 889 810 397 622
Fragments arranged by size:
889 810 622 397
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2022 2281 2718
Size: 2022 259 437
Fragments arranged by size:
2022 437 259
XmnI GAAnn'nnTTC
Cuts at: 0 1554 2718
Size: 1554 1164
Enzymes that do cut:
AcuI AlfI AlwNI ApaLI ApoI AvaI AvrII BanI
BanII BbeI BbvCI BciVI BglI BglII BlpI Bme1580I
BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI
BseRI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrFI
BsrGI BssSI BstAPI BstYI BtgI EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI HaeII Hin4I
HindIII KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PflMI PfoI PpiI PshAI PvuII
SacI SacII SapI SfiI SfoI SmlI SnaBI SspI
StuI StyI TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI AscI AseI AsiSI
BaeI BamHI BbsI BcgI BclI BfrBI BmgBI BmrI
BmtI BsaI BsaWI BseYI BsgI BsiWI BsmI BsmBI
BspEI BsrBI BssHII BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EciI
EcoO109I FseI FspI FspAI HincII HpaI KpnI MfeI
MluI NdeI NheI NotI NruI NsiI PacI PasI
PciI PmeI PmlI PpuMI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SalI SanDI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SpeI SphI SrfI SwaI
TaqII TstI Tth111I XbaI XhoI XmaI ZraI