(Circular) (Six-base) MAPSORT of: pFN21AE1589.seq Check: 4889 from: 1 to: 7861 pFN21AE1589 7861 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2280 2957 2280 Size: 677 7184 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5671 278 Size: 53 83 186 936 4135 2468 Fragments arranged by size: 4135 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2754 5204 1937 Size: 817 2450 4594 Fragments arranged by size: 4594 2450 817 Acc65I G'GTAC_C Cuts at: 5182 5182 Size: 7861 AclI AA'CG_TT Cuts at: 1563 2086 5988 6361 1563 Size: 523 3902 373 3063 Fragments arranged by size: 3902 3063 523 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2439 4541 4700 5924 7308 7576 1632 Size: 807 2102 159 1224 1384 268 1917 Fragments arranged by size: 2102 1917 1384 1224 807 268 159 AfeI AGC'GCT Cuts at: 4475 4475 Size: 7861 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7664 AflIII A'CryG_T Cuts at: 1203 3368 5158 6760 7737 1203 Size: 2165 1790 1602 977 1327 Fragments arranged by size: 2165 1790 1602 1327 977 AgeI A'CCGG_T Cuts at: 4758 6692 4758 Size: 1934 5927 AhdI GACnn_n'nnGTC Cuts at: 6590 6590 Size: 7861 AleI CACnn'nnGTG Cuts at: 1166 2897 1166 Size: 1731 6130 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2199 1184 Size: 1015 6846 AlwNI CAG_nnn'CTG Cuts at: 4610 5604 7176 4610 Size: 994 1572 5295 Fragments arranged by size: 5295 1572 994 ApaLI G'TGCA_C Cuts at: 2970 3508 5918 7074 2970 Size: 538 2410 1156 3757 Fragments arranged by size: 3757 2410 1156 538 ApoI r'AATT_y Cuts at: 1493 2047 2379 2437 2729 3404 5170 5396 5548 Size: 554 332 58 292 675 1766 226 152 Cuts at: 5548 7657 1493 Size: 2109 1697 Fragments arranged by size: 2109 1766 1697 675 554 332 292 226 152 58 AseI AT'TA_AT Cuts at: 160 1032 6415 160 Size: 872 5383 1606 Fragments arranged by size: 5383 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7861 AvaI C'yCGr_G Cuts at: 1870 1943 2975 4524 5186 1870 Size: 73 1032 1549 662 4545 Fragments arranged by size: 4545 1549 1032 662 73 BamHI G'GATC_C Cuts at: 5191 5558 5191 Size: 367 7494 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2995 5182 6637 618 Size: 325 217 627 1208 2187 1455 1842 Fragments arranged by size: 2187 1842 1455 1208 627 325 217 BanII G_rGCy'C Cuts at: 1391 4648 4995 5180 1391 Size: 3257 347 185 4072 Fragments arranged by size: 4072 3257 347 185 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2934 4099 4547 928 Size: 971 1035 1165 448 4242 Fragments arranged by size: 4242 1165 1035 971 448 BbvCI CC'TCA_GC Cuts at: 3524 3805 4676 3524 Size: 281 871 6709 Fragments arranged by size: 6709 871 281 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6052 6086 1903 Size: 34 4115 34 3678 Fragments arranged by size: 4115 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5753 6963 5753 Size: 1210 6651 BclI T'GATC_A Cuts at: 1548 2485 1548 Size: 937 6924 BfrBI ATG'CAT Cuts at: 3856 3856 Size: 7861 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6472 136 Size: 107 122 71 631 605 4800 1525 Fragments arranged by size: 4800 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7856 7856 Size: 7861 BlpI GC'TnA_GC Cuts at: 4026 4031 4059 5278 4026 Size: 5 28 1219 6609 Fragments arranged by size: 6609 1219 28 5 Bme1580I G_kGCm'C Cuts at: 1790 2974 3512 5922 7078 1790 Size: 1184 538 2410 1156 2573 Fragments arranged by size: 2573 2410 1184 1156 538 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7861 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2554 4161 5592 Size: 416 53 83 399 24 1137 1607 1431 Cuts at: 5592 6550 442 Size: 958 1753 Fragments arranged by size: 1753 1607 1431 1137 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7861 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4539 4539 Size: 7861 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2302 2733 3759 4064 4662 5024 6521 1239 Size: 1063 431 1026 305 598 362 1497 2579 Fragments arranged by size: 2579 1497 1063 1026 598 431 362 305 Bpu10I CC'TnA_GC Cuts at: 3524 3805 4676 3524 Size: 281 871 6709 Fragments arranged by size: 6709 871 281 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2880 5986 6851 7149 2880 Size: 3106 865 298 3592 Fragments arranged by size: 3592 3106 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4907 5146 6524 882 Size: 617 3408 239 1378 2219 Fragments arranged by size: 3408 2219 1378 617 239 BsaAI yAC'GTr Cuts at: 493 1204 2302 5652 493 Size: 711 1098 3350 2702 Fragments arranged by size: 3350 2702 1098 711 BsaBI GATnn'nnATC Cuts at: 3312 7855 3312 Size: 4543 3318 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2313 5668 6050 6685 Size: 53 83 186 936 780 3355 382 635 Cuts at: 6685 275 Size: 1451 Fragments arranged by size: 3355 1451 936 780 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4118 4758 6294 6692 6966 7113 7524 4118 Size: 640 1536 398 274 147 411 4455 Fragments arranged by size: 4455 1536 640 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7636 Fragments arranged by size: 7636 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2297 2372 4862 1186 Size: 1111 75 2490 4185 Fragments arranged by size: 4185 2490 1111 75 BseYI C'CCAG_C Cuts at: 3978 3992 4148 4196 5016 7064 7560 3978 Size: 14 156 48 820 2048 496 4279 Fragments arranged by size: 4279 2048 820 496 156 48 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7288 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3904 4763 5311 6072 7100 1540 Size: 275 186 1903 859 548 761 1028 2301 Fragments arranged by size: 2301 1903 1028 859 761 548 275 186 BsiHKAI G_wGCw'C Cuts at: 2788 2974 3512 4648 4995 5922 6007 7078 2788 Size: 186 538 1136 347 927 85 1071 3571 Fragments arranged by size: 3571 1136 1071 927 538 347 186 85 BsmI GAATG_Cn' Cuts at: 2777 2952 5372 5465 2777 Size: 175 2420 93 5173 Fragments arranged by size: 5173 2420 175 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2320 3175 2320 Size: 855 7006 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2788 2974 3512 4648 4995 5180 5922 Size: 399 998 186 538 1136 347 185 742 Cuts at: 5922 6007 7078 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1136 1071 998 742 538 399 347 186 185 85 BspEI T'CCGG_A Cuts at: 4118 7524 4118 Size: 3406 4455 BspHI T'CATG_A Cuts at: 5028 5750 7480 5028 Size: 722 1730 5409 Fragments arranged by size: 5409 1730 722 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2280 2957 4398 5216 844 Size: 1436 677 1441 818 3489 Fragments arranged by size: 3489 1441 1436 818 677 BsrBI CCG'CTC Cuts at: 1394 1823 5748 1394 Size: 429 3925 3507 Fragments arranged by size: 3925 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2106 2566 3009 3642 4356 6356 6530 Size: 1183 858 460 443 633 714 2000 174 Cuts at: 6530 65 Size: 1396 Fragments arranged by size: 2000 1396 1183 858 714 633 460 443 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3048 4758 6505 6692 1700 Size: 110 1238 1710 1747 187 2869 Fragments arranged by size: 2869 1747 1710 1238 187 110 BsrGI T'GTAC_A Cuts at: 96 3310 3631 96 Size: 3214 321 4326 Fragments arranged by size: 4326 3214 321 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7861 BssSI C'ACGA_G Cuts at: 5921 6933 5921 Size: 1012 6849 BstAPI GCAn_nnn'nTGC Cuts at: 794 3154 4411 794 Size: 2360 1257 4244 Fragments arranged by size: 4244 2360 1257 BstBI TT'CG_AA Cuts at: 6665 6665 Size: 7861 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7861 BstXI CCAn_nnnn'nTGG Cuts at: 2157 2361 4238 2157 Size: 204 1877 5780 Fragments arranged by size: 5780 1877 204 BstYI r'GATC_y Cuts at: 1972 2739 3559 4606 5191 5558 5943 5960 7412 Size: 767 820 1047 585 367 385 17 1452 Cuts at: 7412 7498 7510 7856 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1047 820 767 585 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4338 4886 4338 Size: 548 7313 BtgI C'CryG_G Cuts at: 513 1065 2004 4269 513 Size: 552 939 2265 4105 Fragments arranged by size: 4105 2265 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7122 Fragments arranged by size: 7122 683 56 BtsI GCAGTG_nn' Cuts at: 5377 6171 6191 5377 Size: 794 20 7047 Fragments arranged by size: 7047 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7861 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2863 2898 529 Size: 35 2299 35 5492 Fragments arranged by size: 5492 2299 35 35 DraI TTT'AAA Cuts at: 2613 5165 2613 Size: 2552 5309 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6868 809 Size: 507 5552 1802 Fragments arranged by size: 5552 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3132 4899 5308 Size: 54 1425 50 275 18 1302 1767 409 Cuts at: 5308 6197 7826 8 Size: 889 1629 43 Fragments arranged by size: 1767 1629 1425 1302 889 409 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5308 1537 Size: 275 3496 4090 Fragments arranged by size: 4090 3496 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2453 4044 4062 4425 5791 1353 Size: 1100 1591 18 363 1366 3423 Fragments arranged by size: 3423 1591 1366 1100 363 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2249 6448 6822 6968 387 Size: 1064 138 660 4199 374 146 1280 Fragments arranged by size: 4199 1280 1064 660 374 146 138 EcoICRI GAG'CTC Cuts at: 4646 4993 4646 Size: 347 7514 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2302 2439 2733 3759 4064 4541 4662 Size: 393 670 137 294 1026 305 477 121 Cuts at: 4662 4700 5024 5924 6521 7308 7576 1239 Size: 38 324 900 597 787 268 1524 Fragments arranged by size: 1524 1026 900 787 670 597 477 393 324 305 294 268 137 121 38 EcoNI CCTnn'n_nnAGG Cuts at: 3238 3873 3238 Size: 635 7226 EcoRI G'AATT_C Cuts at: 2379 5170 5548 2379 Size: 2791 378 4692 Fragments arranged by size: 4692 2791 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2791 2791 Size: 7861 FspI TGC'GCA Cuts at: 6367 6367 Size: 7861 HaeII r_GCGC'y Cuts at: 1678 4477 7008 1678 Size: 2799 2531 2531 Fragments arranged by size: 2799 2531 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2247 2279 2470 2502 2729 Size: 32 135 32 675 32 191 32 227 Cuts at: 2729 2731 2761 2763 3512 3544 3551 3583 5059 Size: 2 30 2 749 32 7 32 1476 Cuts at: 5059 5091 6502 6534 6576 6608 1373 Size: 32 1411 32 42 32 2626 Fragments arranged by size: 2626 1476 1411 749 675 227 191 135 42 32 32 32 32 32 32 32 32 32 30 7 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 5205 5451 669 Size: 988 281 3267 246 3079 Fragments arranged by size: 3267 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 3738 4712 748 Size: 2990 974 3897 Fragments arranged by size: 3897 2990 974 HpaI GTT'AAC Cuts at: 5451 5451 Size: 7861 KpnI G_GTAC'C Cuts at: 5186 5186 Size: 7861 MfeI C'AATT_G Cuts at: 5460 5460 Size: 7861 MluI A'CGCG_T Cuts at: 7737 7737 Size: 7861 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2701 4468 6975 7159 1305 Size: 1396 1767 2507 184 2007 Fragments arranged by size: 2507 2007 1767 1396 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3134 4901 10 Size: 54 1425 343 1302 1767 2970 Fragments arranged by size: 2970 1767 1425 1302 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2181 2897 3326 4079 4268 4814 5819 Size: 648 1015 716 429 753 189 546 1005 Cuts at: 5819 6178 6337 518 Size: 359 159 2042 Fragments arranged by size: 2042 1015 1005 753 716 648 546 429 359 189 159 MspA1I CmG'CkG Cuts at: 1606 2012 5277 5954 7102 7347 1606 Size: 406 3265 677 1148 245 2120 Fragments arranged by size: 3265 2120 1148 677 406 245 NaeI GCC'GGC Cuts at: 3050 3050 Size: 7861 NcoI C'CATG_G Cuts at: 513 1065 2004 4269 513 Size: 552 939 2265 4105 Fragments arranged by size: 4105 2265 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7861 NgoMIV G'CCGG_C Cuts at: 3048 3048 Size: 7861 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7861 NotI GC'GGCC_GC Cuts at: 5308 5308 Size: 7861 NsiI A_TGCA'T Cuts at: 3858 3858 Size: 7861 NspI r_CATG'y Cuts at: 1132 1229 2243 3157 3372 5219 6764 1132 Size: 97 1014 914 215 1847 1545 2229 Fragments arranged by size: 2229 1847 1545 1014 914 215 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7861 PciI A'CATG_T Cuts at: 3368 6760 3368 Size: 3392 4469 PflMI CCAn_nnn'nTGG Cuts at: 1108 4426 1108 Size: 3318 4543 PfoI T'CCnGG_A Cuts at: 4041 4041 Size: 7861 PmeI GTTT'AAAC Cuts at: 5165 5165 Size: 7861 PmlI CAC'GTG Cuts at: 2302 2302 Size: 7861 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5930 5962 7443 7475 5930 Size: 32 1481 32 6316 Fragments arranged by size: 6316 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7861 PsiI TTA'TAA Cuts at: 5431 5431 Size: 7861 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2071 2103 2071 Size: 32 7829 PstI C_TGCA'G Cuts at: 830 4615 5213 830 Size: 3785 598 3478 Fragments arranged by size: 3785 3478 598 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7861 PvuII CAG'CTG Cuts at: 2012 2012 Size: 7861 SacI G_AGCT'C Cuts at: 4648 4995 4648 Size: 347 7514 SalI G'TCGA_C Cuts at: 1936 5203 1936 Size: 3267 4594 SapI GCTCTTCn'nnn_ Cuts at: 2453 4062 4425 2453 Size: 1609 363 5889 Fragments arranged by size: 5889 1609 363 SbfI CC_TGCA'GG Cuts at: 5213 5213 Size: 7861 ScaI AGT'ACT Cuts at: 2056 3727 6109 7821 2056 Size: 1671 2382 1712 2096 Fragments arranged by size: 2382 2096 1712 1671 SexAI A'CCwGG_T Cuts at: 4230 4230 Size: 7861 SfcI C'TryA_G Cuts at: 826 1045 2755 3958 4377 4611 5209 6344 7025 Size: 219 1710 1203 419 234 598 1135 681 Cuts at: 7025 7216 826 Size: 191 1471 Fragments arranged by size: 1710 1471 1203 1135 681 598 419 234 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7861 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7861 SmaI CCC'GGG Cuts at: 1872 5188 1872 Size: 3316 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2895 4524 5965 6866 7128 820 Size: 197 926 952 1629 1441 901 262 1553 Fragments arranged by size: 1629 1553 1441 952 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5652 493 Size: 5159 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7861 SphI G_CATG'C Cuts at: 1132 3157 5219 1132 Size: 2025 2062 3774 Fragments arranged by size: 3774 2062 2025 SspI AAT'ATT Cuts at: 5 52 5785 5 Size: 47 5733 2081 Fragments arranged by size: 5733 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7861 StyI C'CwwG_G Cuts at: 513 1065 2004 4269 5300 513 Size: 552 939 2265 1031 3074 Fragments arranged by size: 3074 2265 1031 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2364 3890 4955 4976 5888 5905 6058 Size: 1255 784 1526 1065 21 912 17 153 Cuts at: 6058 6243 6688 325 Size: 185 445 1498 Fragments arranged by size: 1526 1498 1255 1065 912 784 445 185 153 21 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2054 3310 3380 Size: 274 80 33 51 1443 77 1256 70 Cuts at: 3380 3596 3631 3725 5049 6107 7819 96 Size: 216 35 94 1324 1058 1712 138 Fragments arranged by size: 1712 1443 1324 1256 1058 274 216 138 94 80 77 70 51 35 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3466 3498 3814 3846 7821 7853 3466 Size: 32 316 32 3975 32 3474 Fragments arranged by size: 3975 3474 316 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7861 XbaI T'CTAG_A Cuts at: 2524 3613 5197 2524 Size: 1089 1584 5188 Fragments arranged by size: 5188 1584 1089 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2358 2358 Size: 7861 XhoI C'TCGA_G Cuts at: 1943 4524 1943 Size: 2581 5280 XmaI C'CCGG_G Cuts at: 1870 5186 1870 Size: 3316 4545 XmnI GAAnn'nnTTC Cuts at: 2441 2721 4497 5990 2441 Size: 280 1776 1493 4312 Fragments arranged by size: 4312 1776 1493 280 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5669 276 Size: 53 83 186 936 4135 2468 Fragments arranged by size: 4135 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BsiWI BstZ17I DraIII EcoO109I EcoRV FseI FspAI KasI NarI NruI PacI PpuMI PspOMI PspXI RsrII SacII SanDI SfiI SfoI SrfI SwaI