Restriction map for
pFN21AE1589
(Linear) (Six-base) MAPSORT of: KIEE1589.seq Check: 676 from: 1 to: 3156
KIEE1589.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 275 952 3156
Size: 275 677 2204
Fragments arranged by size:
2204 677 275
AccI GT'mk_AC
Cuts at: 0 749 3156
Size: 749 2407
AclI AA'CG_TT
Cuts at: 0 81 3156
Size: 81 3075
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 434 2536 2695 3156
Size: 434 2102 159 461
Fragments arranged by size:
2102 461 434 159
AfeI AGC'GCT
Cuts at: 0 2470 3156
Size: 2470 686
AflIII A'CryG_T
Cuts at: 0 1363 3156
Size: 1363 1793
AgeI A'CCGG_T
Cuts at: 0 2753 3156
Size: 2753 403
AleI CACnn'nnGTG
Cuts at: 0 892 3156
Size: 892 2264
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 194 3156
Size: 194 2962
AlwNI CAG_nnn'CTG
Cuts at: 0 2605 3156
Size: 2605 551
ApaLI G'TGCA_C
Cuts at: 0 965 1503 3156
Size: 965 538 1653
Fragments arranged by size:
1653 965 538
ApoI r'AATT_y
Cuts at: 0 42 374 432 724 1399 3156
Size: 42 332 58 292 675 1757
Fragments arranged by size:
1757 675 332 292 58 42
AvaI C'yCGr_G
Cuts at: 0 970 2519 3156
Size: 970 1549 637
Fragments arranged by size:
1549 970 637
BanI G'GyrC_C
Cuts at: 0 990 3156
Size: 990 2166
BanII G_rGCy'C
Cuts at: 0 2643 2990 3156
Size: 2643 347 166
Fragments arranged by size:
2643 347 166
BbsI GAAGACnn'nnnn_
Cuts at: 0 929 2094 2542 3156
Size: 929 1165 448 614
Fragments arranged by size:
1165 929 614 448
BbvCI CC'TCA_GC
Cuts at: 0 1519 1800 2671 3156
Size: 1519 281 871 485
Fragments arranged by size:
1519 871 485 281
BclI T'GATC_A
Cuts at: 0 480 3156
Size: 480 2676
BfrBI ATG'CAT
Cuts at: 0 1851 3156
Size: 1851 1305
BlpI GC'TnA_GC
Cuts at: 0 2021 2026 2054 3156
Size: 2021 5 28 1102
Fragments arranged by size:
2021 1102 28 5
Bme1580I G_kGCm'C
Cuts at: 0 969 1507 3156
Size: 969 538 1649
Fragments arranged by size:
1649 969 538
BmrI ACTGGGnnnn_n'
Cuts at: 0 549 2156 3156
Size: 549 1607 1000
Fragments arranged by size:
1607 1000 549
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2534 3156
Size: 2534 622
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 297 728 1754 2059 2657 3019 3156
Size: 297 431 1026 305 598 362 137
Fragments arranged by size:
1026 598 431 362 305 297 137
Bpu10I CC'TnA_GC
Cuts at: 0 1519 1800 2671 3156
Size: 1519 281 871 485
Fragments arranged by size:
1519 871 485 281
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 875 3156
Size: 875 2281
BsaI GGTCTCn'nnnn_
Cuts at: 0 2902 3141 3156
Size: 2902 239 15
Fragments arranged by size:
2902 239 15
BsaAI yAC'GTr
Cuts at: 0 297 3156
Size: 297 2859
BsaBI GATnn'nnATC
Cuts at: 0 1307 3156
Size: 1307 1849
BsaHI Gr'CG_yC
Cuts at: 0 308 3156
Size: 308 2848
BsaWI w'CCGG_w
Cuts at: 0 2113 2753 3156
Size: 2113 640 403
Fragments arranged by size:
2113 640 403
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 292 367 2857 3156
Size: 292 75 2490 299
Fragments arranged by size:
2490 299 292 75
BseYI C'CCAG_C
Cuts at: 0 1973 1987 2143 2191 3011 3156
Size: 1973 14 156 48 820 145
Fragments arranged by size:
1973 820 156 145 48 14
BsiEI CG_ry'CG
Cuts at: 0 1899 2758 3156
Size: 1899 859 398
Fragments arranged by size:
1899 859 398
BsiHKAI G_wGCw'C
Cuts at: 0 783 969 1507 2643 2990 3156
Size: 783 186 538 1136 347 166
Fragments arranged by size:
1136 783 538 347 186 166
BsmI GAATG_Cn'
Cuts at: 0 772 947 3156
Size: 772 175 2209
Fragments arranged by size:
2209 772 175
BsmBI CGTCTCn'nnnn_
Cuts at: 0 315 1170 3156
Size: 315 855 1986
Fragments arranged by size:
1986 855 315
Bsp1286I G_dGCh'C
Cuts at: 0 783 969 1507 2643 2990 3156
Size: 783 186 538 1136 347 166
Fragments arranged by size:
1136 783 538 347 186 166
BspEI T'CCGG_A
Cuts at: 0 2113 3156
Size: 2113 1043
BspHI T'CATG_A
Cuts at: 0 3023 3156
Size: 3023 133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 275 952 2393 3156
Size: 275 677 1441 763
Fragments arranged by size:
1441 763 677 275
BsrDI GCAATG_nn'
Cuts at: 0 101 561 1004 1637 2351 3156
Size: 101 460 443 633 714 805
Fragments arranged by size:
805 714 633 460 443 101
BsrFI r'CCGG_y
Cuts at: 0 1043 2753 3156
Size: 1043 1710 403
Fragments arranged by size:
1710 1043 403
BsrGI T'GTAC_A
Cuts at: 0 1305 1626 3156
Size: 1305 321 1530
Fragments arranged by size:
1530 1305 321
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1149 2406 3156
Size: 1149 1257 750
Fragments arranged by size:
1257 1149 750
BstXI CCAn_nnnn'nTGG
Cuts at: 0 152 356 2233 3156
Size: 152 204 1877 923
Fragments arranged by size:
1877 923 204 152
BstYI r'GATC_y
Cuts at: 0 734 1554 2601 3156
Size: 734 820 1047 555
Fragments arranged by size:
1047 820 734 555
Bsu36I CC'TnA_GG
Cuts at: 0 2333 2881 3156
Size: 2333 548 275
Fragments arranged by size:
2333 548 275
BtgI C'CryG_G
Cuts at: 0 2264 3156
Size: 2264 892
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 858 893 3156
Size: 858 35 2263
Fragments arranged by size:
2263 858 35
DraI TTT'AAA
Cuts at: 0 608 3156
Size: 608 2548
EaeI y'GGCC_r
Cuts at: 0 1127 2894 3156
Size: 1127 1767 262
Fragments arranged by size:
1767 1127 262
EarI CTCTTCn'nnn_
Cuts at: 0 448 2039 2057 2420 3156
Size: 448 1591 18 363 736
Fragments arranged by size:
1591 736 448 363 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 244 3156
Size: 244 2912
EcoICRI GAG'CTC
Cuts at: 0 2641 2988 3156
Size: 2641 347 168
Fragments arranged by size:
2641 347 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 297 434 728 1754 2059 2536 2657 2695
Size: 297 137 294 1026 305 477 121 38
Cuts at: 2695 3019 3156
Size: 324 137
Fragments arranged by size:
1026 477 324 305 297 294 137 137
121 38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1233 1868 3156
Size: 1233 635 1288
Fragments arranged by size:
1288 1233 635
EcoRI G'AATT_C
Cuts at: 0 374 3156
Size: 374 2782
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 786 3156
Size: 786 2370
HaeII r_GCGC'y
Cuts at: 0 2472 3156
Size: 2472 684
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 242 274 465 497 724 726 756 758
Size: 242 32 191 32 227 2 30 2
Cuts at: 758 1507 1539 1546 1578 3054 3086 3156
Size: 749 32 7 32 1476 32 70
Fragments arranged by size:
1476 749 242 227 191 70 32 32
32 32 32 30 7 2 2
HindIII A'AGCT_T
Cuts at: 0 1733 2707 3156
Size: 1733 974 449
Fragments arranged by size:
1733 974 449
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 696 2463 3156
Size: 696 1767 693
Fragments arranged by size:
1767 696 693
MscI TGG'CCA
Cuts at: 0 1129 2896 3156
Size: 1129 1767 260
Fragments arranged by size:
1767 1129 260
MslI CAynn'nnrTG
Cuts at: 0 176 892 1321 2074 2263 2809 3156
Size: 176 716 429 753 189 546 347
Fragments arranged by size:
753 716 546 429 347 189 176
MspA1I CmG'CkG
Cuts at: 0 7 3156
Size: 7 3149
NaeI GCC'GGC
Cuts at: 0 1045 3156
Size: 1045 2111
NcoI C'CATG_G
Cuts at: 0 2264 3156
Size: 2264 892
NgoMIV G'CCGG_C
Cuts at: 0 1043 3156
Size: 1043 2113
NsiI A_TGCA'T
Cuts at: 0 1853 3156
Size: 1853 1303
NspI r_CATG'y
Cuts at: 0 238 1152 1367 3156
Size: 238 914 215 1789
Fragments arranged by size:
1789 914 238 215
PciI A'CATG_T
Cuts at: 0 1363 3156
Size: 1363 1793
PflMI CCAn_nnn'nTGG
Cuts at: 0 2421 3156
Size: 2421 735
PfoI T'CCnGG_A
Cuts at: 0 2036 3156
Size: 2036 1120
PmlI CAC'GTG
Cuts at: 0 297 3156
Size: 297 2859
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 66 98 3156
Size: 66 32 3058
Fragments arranged by size:
3058 66 32
PstI C_TGCA'G
Cuts at: 0 2610 3156
Size: 2610 546
PvuII CAG'CTG
Cuts at: 0 7 3156
Size: 7 3149
SacI G_AGCT'C
Cuts at: 0 2643 2990 3156
Size: 2643 347 166
Fragments arranged by size:
2643 347 166
SapI GCTCTTCn'nnn_
Cuts at: 0 448 2057 2420 3156
Size: 448 1609 363 736
Fragments arranged by size:
1609 736 448 363
ScaI AGT'ACT
Cuts at: 0 51 1722 3156
Size: 51 1671 1434
Fragments arranged by size:
1671 1434 51
SexAI A'CCwGG_T
Cuts at: 0 2225 3156
Size: 2225 931
SfcI C'TryA_G
Cuts at: 0 750 1953 2372 2606 3156
Size: 750 1203 419 234 550
Fragments arranged by size:
1203 750 550 419 234
SmlI C'TyrA_G
Cuts at: 0 890 2519 3156
Size: 890 1629 637
Fragments arranged by size:
1629 890 637
SphI G_CATG'C
Cuts at: 0 1152 3156
Size: 1152 2004
StyI C'CwwG_G
Cuts at: 0 2264 3156
Size: 2264 892
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 359 1885 2950 2971 3156
Size: 359 1526 1065 21 185
Fragments arranged by size:
1526 1065 359 185 21
TatI w'GTAC_w
Cuts at: 0 49 1305 1375 1591 1626 1720 3044 3156
Size: 49 1256 70 216 35 94 1324 112
Fragments arranged by size:
1324 1256 216 112 94 70 49 35
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1461 1493 1809 1841 3156
Size: 1461 32 316 32 1315
Fragments arranged by size:
1461 1315 316 32 32
XbaI T'CTAG_A
Cuts at: 0 519 1608 3156
Size: 519 1089 1548
Fragments arranged by size:
1548 1089 519
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 353 3156
Size: 353 2803
XhoI C'TCGA_G
Cuts at: 0 2519 3156
Size: 2519 637
XmnI GAAnn'nnTTC
Cuts at: 0 436 716 2492 3156
Size: 436 280 1776 664
Fragments arranged by size:
1776 664 436 280
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AvaI BanI BanII BbsI
BbvCI BclI BfrBI BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrDI BsrFI BsrGI BstAPI BstXI BstYI Bsu36I
BtgI CspCI DraI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoRI FalI HaeII Hin4I HindIII MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NsiI NspI PciI
PflMI PfoI PmlI PsrI PstI PvuII SacI SapI
ScaI SexAI SfcI SmlI SphI StyI TaqII TatI
TstI XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AflII AhdI AloI ApaI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BglI
BglII BmgBI BmtI BsaXI BsgI BsiWI BsrBI BssHII
BssSI BstBI BstEII BstZ17I BtgZI BtsI ClaI DraIII
DrdI EagI EcoO109I EcoRV FseI FspI FspAI HincII
HpaI KasI KpnI MfeI MluI NarI NdeI NheI
NotI NruI PacI PasI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PvuI RsrII SacII SalI SanDI
SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StuI SwaI Tth111I XmaI ZraI