Restriction map for
pFN21AE3466
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AocI Aor51HI AscI AseI
AsnI Asp718I AspI AviII AvrII
BbeI BbrPI BbvCI BcgI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaHI BseAI BsiWI BsmBI BspEI
Bst1107I BstEII BstPI BstZ17I Bsu36I
CelII CpoI DraIII EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoT22I
FseI FspI Hin1I HindIII HpaI
KasI KpnI MluI MroI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I RsrII SacI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SmlI SnaBI SphI Sse8387I SspI
StuI SwaI Tsp45I Tth111I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AgeI 569 ACCGGT A^CCGGT
AhdI 457 GACATACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1715 GTGCAC G^TGCAC
ApaLI 1715 GTGCAC G^TGCAC
AspEI 457 GACATACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2316 CCCGAG C^[CT]CG[GA]G
BalI 1926 TGGCCA TGG^CCA
BclI 381 TGATCA T^GATCA
BcnI 4004 CCCGG CC^[GC]GG
BsaBI 1240 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 569 ACCGGT [AT]^CCGG[AT]
BsiEI 1639 CGATCG CG[GA][CT]^CG
BsoBI 2316 CCCGAG C^[CT]CG[GA]G
BspDI 1073 ATCGAT AT^CGAT
BsrBI 1656 GAGCGG CCGCTC(-3/-3)
BsrFI 569 ACCGGT [GA]^CCGG[CT]
BssHII 1231 GCGCGC G^CGCGC
BssSI 34 CTCGTG CACGAG(-5/-1)
BstAPI 1577 GCAGTCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 3736 CCAGTGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 569 ACCGGT [GA]^CCGG[CT]
ClaI 1073 ATCGAT AT^CGAT
EaeI 1926 TGGCCA [CT]^GGCC[GA]
Eam1105I 457 GACATACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 231 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 381 TGATCA T^GATCA
HaeII 532 AGCGCC [GA]GCGC^[CT]
HgaI 404 GCGTC GACGC(5/10)
KspI 140 CCGCGG CCGC^GG
MaeIII 2210 GTTAC ^GT[ACGT]AC
MamI 1240 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2072 CAATTG C^AATTG
MluNI 1926 TGGCCA TGG^CCA
MscI 1926 TGGCCA TGG^CCA
MunI 2072 CAATTG C^AATTG
NciI 4004 CCCGG CC^[GC]GG
NdeI 1746 CATATG CA^TATG
PflMI 1540 CCATTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 569 ACCGGT A^CCGGT
PpuMI 415 GGGACCT [GA]G^G[AT]CC[CT]
PvuI 1639 CGATCG CGAT^CG
PvuII 2422 CAGCTG CAG^CTG
SacII 140 CCGCGG CCGC^GG
SalI 364 GTCGAC G^TCGAC
SapI 499 GAAGAGC GCTCTTC(1/4)
SpeI 3106 ACTAGT A^CTAGT
Van91I 1540 CCATTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 4147 CCAGCCTCCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 364 GTCGAC GT^[AC][GT]AC
3001 GTAGAC
AlwNI 837 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1052 CAGATTCTG
ApaI 977 GGGCCC GGGCC^C
3214 GGGCCC
Asp700I 226 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2899 GAATTAGTTC
BamHI 1965 GGATCC G^GATCC
2474 GGATCC
BciVI 3075 GTATCC GTATCC(6/5)
4092 GTATCC
BsaI 1343 GAGACC GGTCTC(1/5)
3452 GGTCTC
Bsp1407I 2505 TGTACA T^GTACA
3708 TGTACA
BspHI 1179 TCATGA T^CATGA
3389 TCATGA
BsrGI 2505 TGTACA T^GTACA
3708 TGTACA
BstBI 3494 TTCGAA TT^CGAA
3506 TTCGAA
DrdI 687 GACGGACCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3084 GACAGACATGTC
DsaI 140 CCGCGG C^C[GA][CT]GG
1929 CCACGG
EarI 499 GAAGAG CTCTTC(1/4)
3254 GAAGAG
EcoRI 2055 GAATTC G^AATTC
2301 GAATTC
EcoRV 639 GATATC GAT^ATC
4008 GATATC
FauI 10 CCCGC CCCGC(4/6)
1658 GCGGG
HapII 570 CCGG C^CGG
4005 CCGG
HpaII 570 CCGG C^CGG
4005 CCGG
Ksp632I 499 GAAGAG CTCTTC(1/4)
3254 GAAGAG
MaeII 3857 ACGT A^CGT
4073 ACGT
MspI 570 CCGG C^CGG
4005 CCGG
NspV 3494 TTCGAA TT^CGAA
3506 TTCGAA
RcaI 1179 TCATGA T^CATGA
3389 TCATGA
SfuI 3494 TTCGAA TT^CGAA
3506 TTCGAA
SspBI 2505 TGTACA T^GTACA
3708 TGTACA
XbaI 804 TCTAGA T^CTAGA
2059 TCTAGA
XmnI 226 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2899 GAATTAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AspHI AvaII
BanI BanII BbsI BbvI BfaI
BglII BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspLU11I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HhaI HinP1I HincII HindII
HinfI HphI ItaI MaeI MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MvnI MwoI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII