Restriction map for
pFN21AE3466
(Linear) (Six-base) MAPSORT of: KIEE3466.seq Check: 8423 from: 1 to: 4170
KIEE3466.orf
With 182 enzymes: SgfI *
August 1, 2019 16:47 ..
AccI GT'mk_AC
Cuts at: 0 365 3002 4170
Size: 365 2637 1168
Fragments arranged by size:
2637 1168 365
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 934 2190 2621 2687 4170
Size: 217 717 1256 431 66 1483
Fragments arranged by size:
1483 1256 717 431 217 66
AflIII A'CryG_T
Cuts at: 0 429 2086 3089 3683 4170
Size: 429 1657 1003 594 487
Fragments arranged by size:
1657 1003 594 487 429
AgeI A'CCGG_T
Cuts at: 0 569 4170
Size: 569 3601
AhdI GACnn_n'nnGTC
Cuts at: 0 462 4170
Size: 462 3708
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 52 4170
Size: 52 4118
AlwNI CAG_nnn'CTG
Cuts at: 0 842 1057 4170
Size: 842 215 3113
Fragments arranged by size:
3113 842 215
ApaI G_GGCC'C
Cuts at: 0 981 3218 4170
Size: 981 2237 952
Fragments arranged by size:
2237 981 952
ApaLI G'TGCA_C
Cuts at: 0 1715 4170
Size: 1715 2455
ApoI r'AATT_y
Cuts at: 0 603 1212 1431 1783 2055 2237 2301 2787
Size: 603 609 219 352 272 182 64 486
Cuts at: 2787 3311 3498 3643 4170
Size: 524 187 145 527
Fragments arranged by size:
609 603 527 524 486 352 272 219
187 182 145 64
AvaI C'yCGr_G
Cuts at: 0 2316 4170
Size: 2316 1854
BamHI G'GATC_C
Cuts at: 0 1965 2474 4170
Size: 1965 509 1696
Fragments arranged by size:
1965 1696 509
BanI G'GyrC_C
Cuts at: 0 193 1619 3959 4170
Size: 193 1426 2340 211
Fragments arranged by size:
2340 1426 211 193
BanII G_rGCy'C
Cuts at: 0 506 981 3218 3843 4170
Size: 506 475 2237 625 327
Fragments arranged by size:
2237 625 506 475 327
BbsI GAAGACnn'nnnn_
Cuts at: 0 233 3547 3906 4170
Size: 233 3314 359 264
Fragments arranged by size:
3314 359 264 233
BciVI GTATCCnnnnn_n'
Cuts at: 0 3086 4103 4170
Size: 3086 1017 67
Fragments arranged by size:
3086 1017 67
BclI T'GATC_A
Cuts at: 0 381 4170
Size: 381 3789
BglII A'GATC_T
Cuts at: 0 1676 2886 3489 3537 4170
Size: 1676 1210 603 48 633
Fragments arranged by size:
1676 1210 633 603 48
Bme1580I G_kGCm'C
Cuts at: 0 981 1719 2958 3218 4170
Size: 981 738 1239 260 952
Fragments arranged by size:
1239 981 952 738 260
BmrI ACTGGGnnnn_n'
Cuts at: 0 266 1526 3212 4170
Size: 266 1260 1686 958
Fragments arranged by size:
1686 1260 958 266
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 907 1141 1445 4170
Size: 907 234 304 2725
Fragments arranged by size:
2725 907 304 234
Bpu10I CC'TnA_GC
Cuts at: 0 1654 4170
Size: 1654 2516
BsaI GGTCTCn'nnnn_
Cuts at: 0 1337 3458 4170
Size: 1337 2121 712
Fragments arranged by size:
2121 1337 712
BsaBI GATnn'nnATC
Cuts at: 0 1244 4170
Size: 1244 2926
BsaWI w'CCGG_w
Cuts at: 0 569 4170
Size: 569 3601
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 533 563 4170
Size: 533 30 3607
Fragments arranged by size:
3607 533 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 75 2921 3805 3851 4170
Size: 75 2846 884 46 319
Fragments arranged by size:
2846 884 319 75 46
BseYI C'CCAG_C
Cuts at: 0 505 980 1754 2133 4170
Size: 505 475 774 379 2037
Fragments arranged by size:
2037 774 505 475 379
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 1812 3091 3217 4170
Size: 76 1736 1279 126 953
Fragments arranged by size:
1736 1279 953 126 76
BsiEI CG_ry'CG
Cuts at: 0 1642 4170
Size: 1642 2528
BsiHKAI G_wGCw'C
Cuts at: 0 41 1187 1719 1852 2275 3171 3815 4043
Size: 41 1146 532 133 423 896 644 228
Cuts at: 4043 4170
Size: 127
Fragments arranged by size:
1146 896 644 532 423 228 133 127
41
BsmI GAATG_Cn'
Cuts at: 0 633 864 1090 1654 4170
Size: 633 231 226 564 2516
Fragments arranged by size:
2516 633 564 231 226
Bsp1286I G_dGCh'C
Cuts at: 0 41 506 981 1187 1719 1852 2275 2958
Size: 41 465 475 206 532 133 423 683
Cuts at: 2958 3171 3218 3815 3843 4043 4170
Size: 213 47 597 28 200 127
Fragments arranged by size:
683 597 532 475 465 423 213 206
200 133 127 47 41 28
BspHI T'CATG_A
Cuts at: 0 1179 3389 4170
Size: 1179 2210 781
Fragments arranged by size:
2210 1179 781
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 427 1380 2705 4170
Size: 427 953 1325 1465
Fragments arranged by size:
1465 1325 953 427
BsrBI CCG'CTC
Cuts at: 0 1658 4170
Size: 1658 2512
BsrDI GCAATG_nn'
Cuts at: 0 72 628 2565 4170
Size: 72 556 1937 1605
Fragments arranged by size:
1937 1605 556 72
BsrFI r'CCGG_y
Cuts at: 0 569 4170
Size: 569 3601
BsrGI T'GTAC_A
Cuts at: 0 2505 3708 4170
Size: 2505 1203 462
Fragments arranged by size:
2505 1203 462
BssHII G'CGCG_C
Cuts at: 0 1231 4170
Size: 1231 2939
BssSI C'ACGA_G
Cuts at: 0 34 4170
Size: 34 4136
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1583 4170
Size: 1583 2587
BstBI TT'CG_AA
Cuts at: 0 3495 3507 4170
Size: 3495 12 663
Fragments arranged by size:
3495 663 12
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3743 4170
Size: 3743 427
BstYI r'GATC_y
Cuts at: 0 919 1535 1676 1965 2220 2474 2886 3489
Size: 919 616 141 289 255 254 412 603
Cuts at: 3489 3537 4170
Size: 48 633
Fragments arranged by size:
919 633 616 603 412 289 255 254
141 48
BtgI C'CryG_G
Cuts at: 0 140 1929 4170
Size: 140 1789 2241
Fragments arranged by size:
2241 1789 140
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2359 4170
Size: 2359 1811
BtsI GCAGTG_nn'
Cuts at: 0 474 1185 2901 3200 4170
Size: 474 711 1716 299 970
Fragments arranged by size:
1716 970 711 474 299
ClaI AT'CG_AT
Cuts at: 0 1074 4170
Size: 1074 3096
DraI TTT'AAA
Cuts at: 0 218 1793 2096 4170
Size: 218 1575 303 2074
Fragments arranged by size:
2074 1575 303 218
DrdI GACnn_nn'nnGTC
Cuts at: 0 693 3090 4170
Size: 693 2397 1080
Fragments arranged by size:
2397 1080 693
EaeI y'GGCC_r
Cuts at: 0 1926 4170
Size: 1926 2244
EarI CTCTTCn'nnn_
Cuts at: 0 494 3249 4170
Size: 494 2755 921
Fragments arranged by size:
2755 921 494
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 907 934 1141 1445 2190 2621 2687
Size: 217 690 27 207 304 745 431 66
Cuts at: 2687 4170
Size: 1483
Fragments arranged by size:
1483 745 690 431 304 217 207 66
27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 235 4170
Size: 235 3935
EcoO109I rG'GnC_Cy
Cuts at: 0 6 416 977 4170
Size: 6 410 561 3193
Fragments arranged by size:
3193 561 410 6
EcoRI G'AATT_C
Cuts at: 0 2055 2301 4170
Size: 2055 246 1869
Fragments arranged by size:
2055 1869 246
EcoRV GAT'ATC
Cuts at: 0 641 4010 4170
Size: 641 3369 160
Fragments arranged by size:
3369 641 160
HaeII r_GCGC'y
Cuts at: 0 536 4170
Size: 536 3634
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 448 480 533 565 1653 1685 3507 3539
Size: 448 32 53 32 1088 32 1822 32
Cuts at: 3539 3784 3816 4170
Size: 245 32 354
Fragments arranged by size:
1822 1088 448 354 245 53 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 366 408 1110 1134 1281 2371 4170
Size: 366 42 702 24 147 1090 1799
Fragments arranged by size:
1799 1090 702 366 147 42 24
MfeI C'AATT_G
Cuts at: 0 2072 4170
Size: 2072 2098
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 2269 2610 3272 3550 4170
Size: 446 1823 341 662 278 620
Fragments arranged by size:
1823 662 620 446 341 278
MscI TGG'CCA
Cuts at: 0 1928 4170
Size: 1928 2242
MslI CAynn'nnrTG
Cuts at: 0 34 349 877 1866 2332 3239 3410 4170
Size: 34 315 528 989 466 907 171 760
Fragments arranged by size:
989 907 760 528 466 315 171 34
MspA1I CmG'CkG
Cuts at: 0 13 142 2424 3459 4170
Size: 13 129 2282 1035 711
Fragments arranged by size:
2282 1035 711 129 13
NdeI CA'TA_TG
Cuts at: 0 1747 4170
Size: 1747 2423
NspI r_CATG'y
Cuts at: 0 433 2090 2369 3093 3205 3687 4170
Size: 433 1657 279 724 112 482 483
Fragments arranged by size:
1657 724 483 482 433 279 112
PasI CC'CwG_GG
Cuts at: 0 537 4170
Size: 537 3633
PciI A'CATG_T
Cuts at: 0 429 2086 3089 3683 4170
Size: 429 1657 1003 594 487
Fragments arranged by size:
1657 1003 594 487 429
PflMI CCAn_nnn'nTGG
Cuts at: 0 1546 4170
Size: 1546 2624
PfoI T'CCnGG_A
Cuts at: 0 1439 4003 4170
Size: 1439 2564 167
Fragments arranged by size:
2564 1439 167
PpuMI rG'GwC_Cy
Cuts at: 0 416 4170
Size: 416 3754
PspOMI G'GGCC_C
Cuts at: 0 977 3214 4170
Size: 977 2237 956
Fragments arranged by size:
2237 977 956
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1242 1274 4170
Size: 1242 32 2896
Fragments arranged by size:
2896 1242 32
PstI C_TGCA'G
Cuts at: 0 304 1561 1579 4170
Size: 304 1257 18 2591
Fragments arranged by size:
2591 1257 304 18
PvuI CG_AT'CG
Cuts at: 0 1642 4170
Size: 1642 2528
PvuII CAG'CTG
Cuts at: 0 2424 4170
Size: 2424 1746
SacII CC_GC'GG
Cuts at: 0 143 4170
Size: 143 4027
SalI G'TCGA_C
Cuts at: 0 364 4170
Size: 364 3806
SapI GCTCTTCn'nnn_
Cuts at: 0 494 4170
Size: 494 3676
SfcI C'TryA_G
Cuts at: 0 300 1557 1575 2975 2999 3702 4170
Size: 300 1257 18 1400 24 703 468
Fragments arranged by size:
1400 1257 703 468 300 24 18
SpeI A'CTAG_T
Cuts at: 0 3106 4170
Size: 3106 1064
StyI C'CwwG_G
Cuts at: 0 50 189 1812 2757 4170
Size: 50 139 1623 945 1413
Fragments arranged by size:
1623 1413 945 139 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 165 290 3391 4170
Size: 165 125 3101 779
Fragments arranged by size:
3101 779 165 125
TatI w'GTAC_w
Cuts at: 0 248 1948 2089 2505 2568 2922 3708 4170
Size: 248 1700 141 416 63 354 786 462
Fragments arranged by size:
1700 786 462 416 354 248 141 63
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 14 46 1863 1895 4170
Size: 14 32 1817 32 2275
Fragments arranged by size:
2275 1817 32 32 14
XbaI T'CTAG_A
Cuts at: 0 804 2059 4170
Size: 804 1255 2111
Fragments arranged by size:
2111 1255 804
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 4154 4170
Size: 4154 16
XmnI GAAnn'nnTTC
Cuts at: 0 230 2903 4170
Size: 230 2673 1267
Fragments arranged by size:
2673 1267 230
Enzymes that do cut:
AccI AcuI AflIII AgeI AhdI AlfI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbsI BciVI
BclI BglII Bme1580I BmrI BpmI Bpu10I BsaI BsaBI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EarI Eco57MI EcoNI EcoO109I
EcoRI EcoRV HaeII Hin4I HincII MfeI MmeI MscI
MslI MspA1I NdeI NspI PasI PciI PflMI PfoI
PpuMI PspOMI PsrI PstI PvuI PvuII SacII SalI
SapI SfcI SpeI StyI TaqII TatI TstI XbaI
XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AleI AloI
AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI
BfrBI BglI BlpI BmgBI BmtI BplI BpuEI BsaAI
BsaHI BsiWI BsmBI BspEI BstEII BstZ17I Bsu36I CspCI
DraIII EagI EciI EcoICRI FalI FseI FspI FspAI
HindIII HpaI KasI KpnI MluI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI
PpiI PshAI PsiI PspXI RsrII SacI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SphI SrfI SspI StuI SwaI Tth111I XhoI
XmaI ZraI