################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## Acc65I AclI AflII AgeI AscI AseI AsnI Asp718I AviII AvrII BbeI BbrPI BcgI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsiWI Bsp1407I BspHI BsrDI BsrGI BssHII Bst1107I BstAPI BstZ17I CelII CpoI DraIII EcoNI EcoT22I FseI FspI HindIII KasI KpnI KspI MfeI MluI MunI NarI NdeI NheI NruI NsiI PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI SspBI SwaI XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 2554 GACGTC GACGT^C AcyI 2554 GACGTC G[GA]^CG[CT]C AflIII 1935 ACATGT A^C[GA][CT]GT AhdI 193 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 215 GTGCAC G^TGCAC AlwNI 1018 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 337 CCTAAGG CC^T[ACGT]AGG Aor51HI 2501 AGCGCT AGC^GCT ApaI 1507 GGGCCC GGGCC^C ApaLI 215 GTGCAC G^TGCAC AspEI 193 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 1577 TGGCCA TGG^CCA BamHI 2732 GGATCC G^GATCC BbvCI 963 CCTCAGC CCTCAGC(-5/-2) BglI 46 GCCCGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 1179 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2554 GACGTC G[GA]^CG[CT]C BsmBI 2764 CGTCTC CGTCTC(1/5) BspLU11I 1935 ACATGT A^CATGT BssSI 56 CACGAG CACGAG(-5/-1) BstEII 1980 GGTTACC G^GT[ACGT]ACC BstPI 1980 GGTTACC G^GT[ACGT]ACC Bsu36I 337 CCTAAGG CC^T[ACGT]AGG DrdI 2548 GACGAGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 193 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2501 AGCGCT AGC^GCT Eco81I 337 CCTAAGG CC^T[ACGT]AGG EcoO65I 1980 GGTTACC G^GT[ACGT]ACC EcoRI 1701 GAATTC G^AATTC EcoRV 154 GATATC GAT^ATC Hin1I 2554 GACGTC G[GA]^CG[CT]C HpaI 636 GTTAAC GTT^AAC MamI 1179 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1577 TGGCCA TGG^CCA MscI 1577 TGGCCA TGG^CCA NaeI 1817 GCCGGC GCC^GGC NgoMIV 1817 GCCGGC G^CCGGC NotI 3080 GCGGCCGC GC^GGCCGC PaeR7I 21 CTCGAG C^TCGAG PpuMI 371 AGGACCT [GA]G^G[AT]CC[CT] SacI 1501 GAGCTC GAGCT^C SbfI 456 CCTGCAGG CCTGCA^GG Sse8387I 456 CCTGCAGG CCTGCA^GG SspI 1324 AATATT AAT^ATT StuI 1333 AGGCCT AGG^CCT XhoI 21 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AccII 1607 CGCG CG^CG 3154 CGCG Asp700I 547 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2581 GAACTGATTC AspI 656 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2535 GACGCTGTC BclI 1139 TGATCA T^GATCA 2175 TGATCA BcnI 3020 CCGGG CC^[GC]GG 3045 CCGGG BfaI 703 CTAG C^TAG 1868 CTAG BsgI 725 CTGCAC GTGCAG(16/14) 73 GTGCAG BsiEI 751 CGGCCG CG[GA][CT]^CG 3081 CGGCCG BsmI 1537 GAATGC GAATGC(1/-1) 2660 GAATGC BspDI 37 ATCGAT AT^CGAT 884 ATCGAT BsrFI 1817 GCCGGC [GA]^CCGG[CT] 2057 GCCGGT BstBI 1970 TTCGAA TT^CGAA 2657 TTCGAA BstUI 1607 CGCG CG^CG 3154 CGCG BstXI 2063 CCAGGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2406 CCAACTGCTTGG Cfr10I 1817 GCCGGC [GA]^CCGG[CT] 2057 GCCGGT ClaI 37 ATCGAT AT^CGAT 884 ATCGAT DraI 181 TTTAAA TTT^AAA 2805 TTTAAA DraII 371 AGGACCT [GA]G^G[ACGT]CC[CT] 1507 GGGCCCC EagI 751 CGGCCG C^GGCCG 3081 CGGCCG EclXI 751 CGGCCG C^GGCCG 3081 CGGCCG Eco52I 751 CGGCCG C^GGCCG 3081 CGGCCG EcoO109I 371 AGGACCT [GA]G^G[ACGT]CC[CT] 1507 GGGCCCC FbaI 1139 TGATCA T^GATCA 2175 TGATCA MaeI 703 CTAG C^TAG 1868 CTAG MvnI 1607 CGCG CG^CG 3154 CGCG NciI 3020 CCGGG CC^[GC]GG 3045 CCGGG NcoI 1976 CCATGG C^CATGG 2838 CCATGG NspV 1970 TTCGAA TT^CGAA 2657 TTCGAA PflFI 656 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2535 GACGCTGTC SalI 140 GTCGAC G^TCGAC 1354 GTCGAC SfuI 1970 TTCGAA TT^CGAA 2657 TTCGAA Tth111I 656 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2535 GACGCTGTC XcmI 1565 CCAAGCAACTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2984 CCATCCGCTGCGTGG XmnI 547 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2581 GAACTGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AccI AccIII AciI AcsI AfaI AluI AlwI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseAI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspEI BspMI BsrBI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MroI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII