(Circular) (Six-base) MAPSORT of: pFN21AE4263.seq Check: 8367 from: 1 to: 7909 pFN21AE4263 7909 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5117 5117 Size: 7909 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4563 5719 278 Size: 53 83 186 936 3027 1156 2468 Fragments arranged by size: 3027 2468 1156 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2146 3360 4827 5252 1937 Size: 209 1214 1467 425 4594 Fragments arranged by size: 4594 1467 1214 425 209 Acc65I G'GTAC_C Cuts at: 5230 5230 Size: 7909 AclI AA'CG_TT Cuts at: 1563 6036 6409 1563 Size: 4473 373 3063 Fragments arranged by size: 4473 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3582 4943 5972 7356 7624 1632 Size: 1950 1361 1029 1384 268 1917 Fragments arranged by size: 1950 1917 1384 1361 1029 268 AfeI AGC'GCT Cuts at: 4508 4508 Size: 7909 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7712 AflIII A'CryG_T Cuts at: 1203 3940 6808 7785 1203 Size: 2737 2868 977 1327 Fragments arranged by size: 2868 2737 1327 977 AgeI A'CCGG_T Cuts at: 6740 6740 Size: 7909 AhdI GACnn_n'nnGTC Cuts at: 2203 6638 2203 Size: 4435 3474 AleI CACnn'nnGTG Cuts at: 1166 2580 1166 Size: 1414 6495 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7909 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4463 4495 4463 Size: 32 7877 AlwNI CAG_nnn'CTG Cuts at: 3028 5652 7224 3028 Size: 2624 1572 3713 Fragments arranged by size: 3713 2624 1572 ApaI G_GGCC'C Cuts at: 3516 3516 Size: 7909 ApaLI G'TGCA_C Cuts at: 2220 5966 7122 2220 Size: 3746 1156 3007 Fragments arranged by size: 3746 3007 1156 ApoI r'AATT_y Cuts at: 1493 2532 2861 3706 5218 5444 5596 7705 1493 Size: 1039 329 845 1512 226 152 2109 1697 Fragments arranged by size: 2109 1697 1512 1039 845 329 226 152 AseI AT'TA_AT Cuts at: 160 1032 6463 160 Size: 872 5431 1606 Fragments arranged by size: 5431 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7909 AvaI C'yCGr_G Cuts at: 1870 1943 2026 3509 3839 4157 4730 5234 1870 Size: 73 83 1483 330 318 573 504 4545 Fragments arranged by size: 4545 1483 573 504 330 318 83 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2413 2446 2413 Size: 33 7876 BamHI G'GATC_C Cuts at: 4737 5239 5606 4737 Size: 502 367 7040 Fragments arranged by size: 7040 502 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3175 3615 4422 5230 6685 Size: 325 217 627 1388 440 807 808 1455 Cuts at: 6685 618 Size: 1842 Fragments arranged by size: 1842 1455 1388 808 807 627 440 325 217 BanII G_rGCy'C Cuts at: 1391 2053 2966 3510 3516 5228 1391 Size: 662 913 544 6 1712 4072 Fragments arranged by size: 4072 1712 913 662 544 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2549 4429 4610 928 Size: 971 650 1880 181 4227 Fragments arranged by size: 4227 1880 971 650 181 BbvCI CC'TCA_GC Cuts at: 2969 2969 Size: 7909 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6100 6134 1903 Size: 34 4163 34 3678 Fragments arranged by size: 4163 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5801 7011 5801 Size: 1210 6699 BclI T'GATC_A Cuts at: 1548 3144 4180 1548 Size: 1596 1036 5277 Fragments arranged by size: 5277 1596 1036 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2057 6520 136 Size: 107 122 71 631 605 385 4463 1525 Fragments arranged by size: 4463 1525 631 605 385 122 107 71 BglII A'GATC_T Cuts at: 7904 7904 Size: 7909 BlpI GC'TnA_GC Cuts at: 5326 5326 Size: 7909 Bme1580I G_kGCm'C Cuts at: 1790 2224 3516 3916 4425 5970 7126 1790 Size: 434 1292 400 509 1545 1156 2573 Fragments arranged by size: 2573 1545 1292 1156 509 434 400 BmgBI CAC'GTC Cuts at: 1318 4384 1318 Size: 3066 4843 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3272 4016 5640 Size: 416 53 83 399 24 1855 744 1624 Cuts at: 5640 6598 442 Size: 958 1753 Fragments arranged by size: 1855 1753 1624 958 744 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7909 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3245 4097 4617 4927 6569 1239 Size: 2006 852 520 310 1642 2579 Fragments arranged by size: 2579 2006 1642 852 520 310 Bpu10I CC'TnA_GC Cuts at: 2315 2820 2969 3209 4097 4406 2315 Size: 505 149 240 888 309 5818 Fragments arranged by size: 5818 888 505 309 240 149 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2423 2432 4958 5108 6034 6899 7197 2423 Size: 9 2526 150 926 865 298 3135 Fragments arranged by size: 3135 2526 926 865 298 150 9 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2874 3461 3755 6572 882 Size: 617 1375 587 294 2817 2219 Fragments arranged by size: 2817 2219 1375 617 587 294 BsaAI yAC'GTr Cuts at: 493 1204 5700 493 Size: 711 4496 2702 Fragments arranged by size: 4496 2702 711 BsaBI GATnn'nnATC Cuts at: 3188 7903 3188 Size: 4715 3194 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4560 5716 6098 6733 Size: 53 83 186 936 3027 1156 382 635 Cuts at: 6733 275 Size: 1451 Fragments arranged by size: 3027 1451 1156 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2124 3186 3324 4900 6342 6740 7014 7161 7572 Size: 1062 138 1576 1442 398 274 147 411 Cuts at: 7572 2124 Size: 2461 Fragments arranged by size: 2461 1576 1442 1062 411 398 274 147 138 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2948 2978 4481 4511 4938 Size: 30 165 30 1545 30 1503 30 427 Cuts at: 4938 4968 1178 Size: 30 4119 Fragments arranged by size: 4119 1545 1503 427 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3002 3005 3685 5092 1186 Size: 1816 3 680 1407 4003 Fragments arranged by size: 4003 1816 1407 680 3 BseYI C'CCAG_C Cuts at: 4110 4237 4348 7112 7608 4110 Size: 127 111 2764 496 4411 Fragments arranged by size: 4411 2764 496 127 111 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2099 2715 1163 Size: 573 363 616 6357 Fragments arranged by size: 6357 616 573 363 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2759 5089 5359 6120 7148 1540 Size: 275 186 758 2330 270 761 1028 2301 Fragments arranged by size: 2330 2301 1028 761 758 275 270 186 BsiHKAI G_wGCw'C Cuts at: 2224 3276 3510 3882 5970 6055 7126 2224 Size: 1052 234 372 2088 85 1071 3007 Fragments arranged by size: 3007 2088 1071 1052 372 234 85 BsmI GAATG_Cn' Cuts at: 3548 4671 5420 5513 3548 Size: 1123 749 93 5944 Fragments arranged by size: 5944 1123 749 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4775 4775 Size: 7909 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2053 2224 2966 3276 3510 3516 3882 Size: 399 263 171 742 310 234 6 366 Cuts at: 3882 3916 4425 5228 5970 6055 7126 1391 Size: 34 509 803 742 85 1071 2174 Fragments arranged by size: 2174 1071 803 742 742 509 399 366 310 263 234 171 85 34 6 BspEI T'CCGG_A Cuts at: 2124 3186 3324 7572 2124 Size: 1062 138 4248 2461 Fragments arranged by size: 4248 2461 1062 138 BspHI T'CATG_A Cuts at: 5798 7528 5798 Size: 1730 6179 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3483 4326 5117 5127 5264 844 Size: 2639 843 791 10 137 3489 Fragments arranged by size: 3489 2639 843 791 137 10 BsrBI CCG'CTC Cuts at: 1394 1823 2095 3814 4954 5796 1394 Size: 429 272 1719 1140 842 3507 Fragments arranged by size: 3507 1719 1140 842 429 272 BsrDI GCAATG_nn' Cuts at: 65 1248 6404 6578 65 Size: 1183 5156 174 1396 Fragments arranged by size: 5156 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3822 4062 6553 6740 1700 Size: 110 2012 240 2491 187 2869 Fragments arranged by size: 2869 2491 2012 240 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7909 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7909 BssSI C'ACGA_G Cuts at: 2061 5969 6981 2061 Size: 3908 1012 2989 Fragments arranged by size: 3908 2989 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7909 BstBI TT'CG_AA Cuts at: 3976 4663 6713 3976 Size: 687 2050 5172 Fragments arranged by size: 5172 2050 687 BstEII G'GTnAC_C Cuts at: 1184 3985 1184 Size: 2801 5108 BstXI CCAn_nnnn'nTGG Cuts at: 4075 4418 4075 Size: 343 7566 BstYI r'GATC_y Cuts at: 1972 3189 3862 4737 5239 5606 5991 6008 7460 Size: 1217 673 875 502 367 385 17 1452 Cuts at: 7460 7546 7558 7904 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1217 875 673 502 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2343 2343 Size: 7909 BtgI C'CryG_G Cuts at: 513 1065 2647 3517 3787 3981 4843 513 Size: 552 1582 870 270 194 862 3579 Fragments arranged by size: 3579 1582 870 862 552 270 194 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2582 487 Size: 683 56 1356 5814 Fragments arranged by size: 5814 1356 683 56 BtsI GCAGTG_nn' Cuts at: 5425 6219 6239 5425 Size: 794 20 7095 Fragments arranged by size: 7095 794 20 ClaI AT'CG_AT Cuts at: 1554 2043 2890 1554 Size: 489 847 6573 Fragments arranged by size: 6573 847 489 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7874 DraI TTT'AAA Cuts at: 2188 4812 5213 2188 Size: 2624 401 4884 Fragments arranged by size: 4884 2624 401 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4559 6916 809 Size: 507 3243 2357 1802 Fragments arranged by size: 3243 2357 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2756 3582 5086 Size: 54 1425 50 275 18 926 826 1504 Cuts at: 5086 5356 6245 7874 8 Size: 270 889 1629 43 Fragments arranged by size: 1629 1504 1425 926 889 826 275 270 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2756 5086 5356 1537 Size: 275 944 2330 270 4090 Fragments arranged by size: 4090 2330 944 275 270 EarI CTCTTCn'nnn_ Cuts at: 1353 3278 3539 3558 3672 3887 4206 4331 5141 Size: 1925 261 19 114 215 319 125 810 Cuts at: 5141 5839 1353 Size: 698 3423 Fragments arranged by size: 3423 1925 810 698 319 261 215 125 114 19 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4049 6496 6870 7016 387 Size: 1064 138 2460 2447 374 146 1280 Fragments arranged by size: 2460 2447 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3508 3508 Size: 7909 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3245 3582 4097 4617 4927 4943 5972 Size: 393 1613 337 515 520 310 16 1029 Cuts at: 5972 6569 7356 7624 1239 Size: 597 787 268 1524 Fragments arranged by size: 1613 1524 1029 787 597 520 515 393 337 310 268 16 EcoO109I rG'GnC_Cy Cuts at: 2377 3513 2377 Size: 1136 6773 EcoRI G'AATT_C Cuts at: 3706 5218 5596 3706 Size: 1512 378 6019 Fragments arranged by size: 6019 1512 378 EcoRV GAT'ATC Cuts at: 2161 2161 Size: 7909 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2090 3553 3595 4222 2090 Size: 1463 42 627 5777 Fragments arranged by size: 5777 1463 627 42 FspI TGC'GCA Cuts at: 6415 6415 Size: 7909 HaeII r_GCGC'y Cuts at: 1678 2827 3832 4510 7056 1678 Size: 1149 1005 678 2546 2531 Fragments arranged by size: 2546 2531 1149 1005 678 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2189 2221 4848 4880 4938 Size: 32 135 32 617 32 2627 32 58 Cuts at: 4938 4970 6550 6582 6624 6656 1373 Size: 32 1580 32 42 32 2626 Fragments arranged by size: 2627 2626 1580 617 135 58 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2147 2643 3361 5253 5499 669 Size: 988 281 209 496 718 1892 246 3079 Fragments arranged by size: 3079 1892 988 718 496 281 246 209 HindIII A'AGCT_T Cuts at: 748 748 Size: 7909 HpaI GTT'AAC Cuts at: 2643 5499 2643 Size: 2856 5053 KpnI G_GTAC'C Cuts at: 5234 5234 Size: 7909 MfeI C'AATT_G Cuts at: 5508 5508 Size: 7909 MluI A'CGCG_T Cuts at: 7785 7785 Size: 7909 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2120 3090 4914 4989 7023 7207 1305 Size: 815 970 1824 75 2034 184 2007 Fragments arranged by size: 2034 2007 1824 970 815 184 75 MscI TGG'CCA Cuts at: 10 64 1489 1832 3584 10 Size: 54 1425 343 1752 4335 Fragments arranged by size: 4335 1752 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2580 2780 3590 4136 5183 5867 6226 Size: 648 1414 200 810 546 1047 684 359 Cuts at: 6226 6385 518 Size: 159 2042 Fragments arranged by size: 2042 1414 1047 810 684 648 546 359 200 159 MspA1I CmG'CkG Cuts at: 1606 2102 3157 4694 4938 4995 5325 6002 7150 Size: 496 1055 1537 244 57 330 677 1148 Cuts at: 7150 7395 1606 Size: 245 2120 Fragments arranged by size: 2120 1537 1148 1055 677 496 330 245 244 57 NaeI GCC'GGC Cuts at: 3824 3824 Size: 7909 NcoI C'CATG_G Cuts at: 513 1065 3981 4843 513 Size: 552 2916 862 3579 Fragments arranged by size: 3579 2916 862 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7909 NgoMIV G'CCGG_C Cuts at: 3822 3822 Size: 7909 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7909 NotI GC'GGCC_GC Cuts at: 5086 5356 5086 Size: 270 7639 NspI r_CATG'y Cuts at: 1132 1229 2854 3944 4043 5267 6812 1132 Size: 97 1625 1090 99 1224 1545 2229 Fragments arranged by size: 2229 1625 1545 1224 1090 99 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7909 PciI A'CATG_T Cuts at: 3940 6808 3940 Size: 2868 5041 PflMI CCAn_nnn'nTGG Cuts at: 1108 3112 3489 4651 1108 Size: 2004 377 1162 4366 Fragments arranged by size: 4366 2004 1162 377 PfoI T'CCnGG_A Cuts at: 4067 4594 4067 Size: 527 7382 PmeI GTTT'AAAC Cuts at: 5213 5213 Size: 7909 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5978 6010 7491 7523 5978 Size: 32 1481 32 6364 Fragments arranged by size: 6364 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2377 2377 Size: 7909 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7909 PsiI TTA'TAA Cuts at: 5479 5479 Size: 7909 PspOMI G'GGCC_C Cuts at: 3512 3512 Size: 7909 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3970 4002 3970 Size: 32 7877 PstI C_TGCA'G Cuts at: 830 2154 2466 2634 3162 3370 3444 3930 4323 Size: 1324 312 168 528 208 74 486 393 Cuts at: 4323 4767 5261 830 Size: 444 494 3478 Fragments arranged by size: 3478 1324 528 494 486 444 393 312 208 168 74 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7909 PvuII CAG'CTG Cuts at: 2102 3157 4694 4938 2102 Size: 1055 1537 244 5073 Fragments arranged by size: 5073 1537 1055 244 SacI G_AGCT'C Cuts at: 3510 3510 Size: 7909 SalI G'TCGA_C Cuts at: 1936 2145 3359 5251 1936 Size: 209 1214 1892 4594 Fragments arranged by size: 4594 1892 1214 209 SapI GCTCTTCn'nnn_ Cuts at: 3539 3672 3887 4331 5141 3539 Size: 133 215 444 810 6307 Fragments arranged by size: 6307 810 444 215 133 SbfI CC_TGCA'GG Cuts at: 2466 5261 2466 Size: 2795 5114 ScaI AGT'ACT Cuts at: 6157 7869 6157 Size: 1712 6197 SfcI C'TryA_G Cuts at: 826 1045 2150 2462 2630 3158 3366 3440 3926 Size: 219 1105 312 168 528 208 74 486 Cuts at: 3926 4319 4441 4763 5137 5257 6392 7073 7264 Size: 393 122 322 374 120 1135 681 191 Cuts at: 7264 826 Size: 1471 Fragments arranged by size: 1471 1135 1105 681 528 486 393 374 322 312 219 208 191 168 122 120 74 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7909 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7909 SmaI CCC'GGG Cuts at: 1872 5236 1872 Size: 3364 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2026 2438 2447 4973 5123 6013 Size: 197 926 83 412 9 2526 150 890 Cuts at: 6013 6914 7176 820 Size: 901 262 1553 Fragments arranged by size: 2526 1553 926 901 890 412 262 197 150 83 9 SnaBI TAC'GTA Cuts at: 493 5700 493 Size: 5207 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7909 SphI G_CATG'C Cuts at: 1132 5267 1132 Size: 4135 3774 SspI AAT'ATT Cuts at: 5 52 3331 5833 5 Size: 47 3279 2502 2081 Fragments arranged by size: 3279 2502 2081 47 StuI AGG'CCT Cuts at: 1517 3340 1517 Size: 1823 6086 StyI C'CwwG_G Cuts at: 513 1065 3091 3753 3765 3981 3990 4843 5348 Size: 552 2026 662 12 216 9 853 505 Cuts at: 5348 513 Size: 3074 Fragments arranged by size: 3074 2026 853 662 552 505 216 12 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3035 4306 5936 5953 6106 6291 6736 Size: 1255 1455 1271 1630 17 153 185 445 Cuts at: 6736 325 Size: 1498 Fragments arranged by size: 1630 1498 1455 1271 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3930 6155 7867 Size: 274 80 33 51 1443 1953 2225 1712 Cuts at: 7867 96 Size: 138 Fragments arranged by size: 2225 1953 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2948 2980 3906 3930 3938 3962 4362 4394 7869 Size: 32 926 24 8 24 400 32 3475 Cuts at: 7869 7901 2948 Size: 32 2956 Fragments arranged by size: 3475 2956 926 400 32 32 32 24 24 8 Tth111I GACn'n_nGTC Cuts at: 1316 2664 4543 1316 Size: 1348 1879 4682 Fragments arranged by size: 4682 1879 1348 XbaI T'CTAG_A Cuts at: 5245 5245 Size: 7909 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3577 4996 3577 Size: 1419 6490 XhoI C'TCGA_G Cuts at: 1943 2026 1943 Size: 83 7826 XmaI C'CCGG_G Cuts at: 1870 5234 1870 Size: 3364 4545 XmnI GAAnn'nnTTC Cuts at: 2556 4590 6038 2556 Size: 2034 1448 4427 Fragments arranged by size: 4427 2034 1448 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4561 5717 276 Size: 53 83 186 936 3027 1156 2468 Fragments arranged by size: 3027 2468 1156 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BbeI BfrBI BplI BsiWI BstZ17I DraIII EcoNI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI