Restriction map for pFN21AE4263
 (Linear) (Six-base) MAPSORT of: KIEE4263.seq  Check: 5866  from: 1  to: 3204



KIEE4263.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 18:52  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3112    3204 
   Size:       3112      92
 
AatII G_ACGT'C

Cuts at:      0    2558    3204 
   Size:       2558     646
 
AccI GT'mk_AC

Cuts at:      0     141    1355    2822    3204 
   Size:        141    1214    1467     382

  Fragments arranged by size: 

                1467    1214     382     141
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1577    2938    3204 
   Size:       1577    1361     266

  Fragments arranged by size: 

                1577    1361     266
 
AfeI AGC'GCT

Cuts at:      0    2503    3204 
   Size:       2503     701
 
AflIII A'CryG_T

Cuts at:      0    1935    3204 
   Size:       1935    1269
 
AhdI GACnn_n'nnGTC

Cuts at:      0     198    3204 
   Size:        198    3006
 
AleI CACnn'nnGTG

Cuts at:      0     575    3204 
   Size:        575    2629
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2458    2490    3204 
   Size:       2458      32     714

  Fragments arranged by size: 

                2458     714      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1023    3204 
   Size:       1023    2181
 
ApaI G_GGCC'C

Cuts at:      0    1511    3204 
   Size:       1511    1693
 
ApaLI G'TGCA_C

Cuts at:      0     215    3204 
   Size:        215    2989
 
ApoI r'AATT_y

Cuts at:      0     527     856    1701    3204 
   Size:        527     329     845    1503

  Fragments arranged by size: 

                1503     845     527     329
 
AvaI C'yCGr_G

Cuts at:      0      21    1504    1834    2152    2725    3204 
   Size:         21    1483     330     318     573     479

  Fragments arranged by size: 

                1483     573     479     330     318      21
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     408     441    3204 
   Size:        408      33    2763

  Fragments arranged by size: 

                2763     408      33
 
BamHI G'GATC_C

Cuts at:      0    2732    3204 
   Size:       2732     472
 
BanI G'GyrC_C

Cuts at:      0    1170    1610    2417    3204 
   Size:       1170     440     807     787

  Fragments arranged by size: 

                1170     807     787     440
 
BanII G_rGCy'C

Cuts at:      0      48     961    1505    1511    3204 
   Size:         48     913     544       6    1693

  Fragments arranged by size: 

                1693     913     544      48       6
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     544    2424    2605    3204 
   Size:        544    1880     181     599

  Fragments arranged by size: 

                1880     599     544     181
 
BbvCI CC'TCA_GC

Cuts at:      0     964    3204 
   Size:        964    2240
 
BclI T'GATC_A

Cuts at:      0    1139    2175    3204 
   Size:       1139    1036    1029

  Fragments arranged by size: 

                1139    1036    1029
 
BglI GCCn_nnn'nGGC

Cuts at:      0      52    3204 
   Size:         52    3152
 
Bme1580I G_kGCm'C

Cuts at:      0     219    1511    1911    2420    3204 
   Size:        219    1292     400     509     784

  Fragments arranged by size: 

                1292     784     509     400     219
 
BmgBI CAC'GTC

Cuts at:      0    2379    3204 
   Size:       2379     825
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1267    2011    3204 
   Size:       1267     744    1193

  Fragments arranged by size: 

                1267    1193     744
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1240    2092    2612    2922    3204 
   Size:       1240     852     520     310     282

  Fragments arranged by size: 

                1240     852     520     310     282
 
Bpu10I CC'TnA_GC

Cuts at:      0     310     815     964    1204    2092    2401    3204 
   Size:        310     505     149     240     888     309     803

  Fragments arranged by size: 

                 888     803     505     310     309     240     149
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     418     427    2953    3103    3204 
   Size:        418       9    2526     150     101

  Fragments arranged by size: 

                2526     418     150     101       9
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     869    1456    1750    3204 
   Size:        869     587     294    1454

  Fragments arranged by size: 

                1454     869     587     294
 
BsaBI GATnn'nnATC

Cuts at:      0    1183    3204 
   Size:       1183    2021
 
BsaHI Gr'CG_yC

Cuts at:      0    2555    3204 
   Size:       2555     649
 
BsaWI w'CCGG_w

Cuts at:      0     119    1181    1319    2895    3204 
   Size:        119    1062     138    1576     309

  Fragments arranged by size: 

                1576    1062     309     138     119
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     943     973    2476    2506    2933    2963    3204 
   Size:        943      30    1503      30     427      30     241

  Fragments arranged by size: 

                1503     943     427     241      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     997    1000    1680    3087    3204 
   Size:        997       3     680    1407     117

  Fragments arranged by size: 

                1407     997     680     117       3
 
BseYI C'CCAG_C

Cuts at:      0    2105    2232    2343    3204 
   Size:       2105     127     111     861

  Fragments arranged by size: 

                2105     861     127     111
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     710    3204 
   Size:         94     616    2494

  Fragments arranged by size: 

                2494     616      94
 
BsiEI CG_ry'CG

Cuts at:      0     754    3084    3204 
   Size:        754    2330     120

  Fragments arranged by size: 

                2330     754     120
 
BsiHKAI G_wGCw'C

Cuts at:      0     219    1271    1505    1877    3204 
   Size:        219    1052     234     372    1327

  Fragments arranged by size: 

                1327    1052     372     234     219
 
BsmI GAATG_Cn'

Cuts at:      0    1543    2666    3204 
   Size:       1543    1123     538

  Fragments arranged by size: 

                1543    1123     538
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2770    3204 
   Size:       2770     434
 
Bsp1286I G_dGCh'C

Cuts at:      0      48     219     961    1271    1505    1511    1877    1911 
   Size:         48     171     742     310     234       6     366      34

Cuts at:   1911    2420    3204 
   Size:        509     784

  Fragments arranged by size: 

                 784     742     509     366     310     234     171      48
                  34       6
 
BspEI T'CCGG_A

Cuts at:      0     119    1181    1319    3204 
   Size:        119    1062     138    1885

  Fragments arranged by size: 

                1885    1062     138     119
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1478    2321    3112    3122    3204 
   Size:       1478     843     791      10      82

  Fragments arranged by size: 

                1478     843     791      82      10
 
BsrBI CCG'CTC

Cuts at:      0      90    1809    2949    3204 
   Size:         90    1719    1140     255

  Fragments arranged by size: 

                1719    1140     255      90
 
BsrFI r'CCGG_y

Cuts at:      0    1817    2057    3204 
   Size:       1817     240    1147

  Fragments arranged by size: 

                1817    1147     240
 
BssSI C'ACGA_G

Cuts at:      0      56    3204 
   Size:         56    3148
 
BstBI TT'CG_AA

Cuts at:      0    1971    2658    3204 
   Size:       1971     687     546

  Fragments arranged by size: 

                1971     687     546
 
BstEII G'GTnAC_C

Cuts at:      0    1980    3204 
   Size:       1980    1224
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2070    2413    3204 
   Size:       2070     343     791

  Fragments arranged by size: 

                2070     791     343
 
BstYI r'GATC_y

Cuts at:      0    1184    1857    2732    3204 
   Size:       1184     673     875     472

  Fragments arranged by size: 

                1184     875     673     472
 
Bsu36I CC'TnA_GG

Cuts at:      0     338    3204 
   Size:        338    2866
 
BtgI C'CryG_G

Cuts at:      0     642    1512    1782    1976    2838    3204 
   Size:        642     870     270     194     862     366

  Fragments arranged by size: 

                 870     862     642     366     270     194
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     577    3204 
   Size:        577    2627
 
ClaI AT'CG_AT

Cuts at:      0      38     885    3204 
   Size:         38     847    2319

  Fragments arranged by size: 

                2319     847      38
 
DraI TTT'AAA

Cuts at:      0     183    2807    3204 
   Size:        183    2624     397

  Fragments arranged by size: 

                2624     397     183
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2554    3204 
   Size:       2554     650
 
EaeI y'GGCC_r

Cuts at:      0     751    1577    3081    3204 
   Size:        751     826    1504     123

  Fragments arranged by size: 

                1504     826     751     123
 
EagI C'GGCC_G

Cuts at:      0     751    3081    3204 
   Size:        751    2330     123

  Fragments arranged by size: 

                2330     751     123
 
EarI CTCTTCn'nnn_

Cuts at:      0    1273    1534    1553    1667    1882    2201    2326    3136 
   Size:       1273     261      19     114     215     319     125     810

Cuts at:   3136    3204 
   Size:         68

  Fragments arranged by size: 

                1273     810     319     261     215     125     114      68
                  19
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2044    3204 
   Size:       2044    1160
 
EcoICRI GAG'CTC

Cuts at:      0    1503    3204 
   Size:       1503    1701
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1240    1577    2092    2612    2922    2938    3204 
   Size:       1240     337     515     520     310      16     266

  Fragments arranged by size: 

                1240     520     515     337     310     266      16
 
EcoO109I rG'GnC_Cy

Cuts at:      0     372    1508    3204 
   Size:        372    1136    1696

  Fragments arranged by size: 

                1696    1136     372
 
EcoRI G'AATT_C

Cuts at:      0    1701    3204 
   Size:       1701    1503
 
EcoRV GAT'ATC

Cuts at:      0     156    3204 
   Size:        156    3048
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      85    1548    1590    2217    3204 
   Size:         85    1463      42     627     987

  Fragments arranged by size: 

                1463     987     627      85      42
 
HaeII r_GCGC'y

Cuts at:      0     822    1827    2505    3204 
   Size:        822    1005     678     699

  Fragments arranged by size: 

                1005     822     699     678
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     184     216    2843    2875    2933    2965    3204 
   Size:        184      32    2627      32      58      32     239

  Fragments arranged by size: 

                2627     239     184      58      32      32      32
 
HincII GTy'rAC

Cuts at:      0     142     638    1356    3204 
   Size:        142     496     718    1848

  Fragments arranged by size: 

                1848     718     496     142
 
HpaI GTT'AAC

Cuts at:      0     638    3204 
   Size:        638    2566
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     115    1085    2909    2984    3204 
   Size:        115     970    1824      75     220

  Fragments arranged by size: 

                1824     970     220     115      75
 
MscI TGG'CCA

Cuts at:      0    1579    3204 
   Size:       1579    1625
 
MslI CAynn'nnrTG

Cuts at:      0     575     775    1585    2131    3178    3204 
   Size:        575     200     810     546    1047      26

  Fragments arranged by size: 

                1047     810     575     546     200      26
 
MspA1I CmG'CkG

Cuts at:      0      97    1152    2689    2933    2990    3204 
   Size:         97    1055    1537     244      57     214

  Fragments arranged by size: 

                1537    1055     244     214      97      57
 
NaeI GCC'GGC

Cuts at:      0    1819    3204 
   Size:       1819    1385
 
NcoI C'CATG_G

Cuts at:      0    1976    2838    3204 
   Size:       1976     862     366

  Fragments arranged by size: 

                1976     862     366
 
NgoMIV G'CCGG_C

Cuts at:      0    1817    3204 
   Size:       1817    1387
 
NotI GC'GGCC_GC

Cuts at:      0    3081    3204 
   Size:       3081     123
 
NspI r_CATG'y

Cuts at:      0     849    1939    2038    3204 
   Size:        849    1090      99    1166

  Fragments arranged by size: 

                1166    1090     849      99
 
PciI A'CATG_T

Cuts at:      0    1935    3204 
   Size:       1935    1269
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1107    1484    2646    3204 
   Size:       1107     377    1162     558

  Fragments arranged by size: 

                1162    1107     558     377
 
PfoI T'CCnGG_A

Cuts at:      0    2062    2589    3204 
   Size:       2062     527     615

  Fragments arranged by size: 

                2062     615     527
 
PpuMI rG'GwC_Cy

Cuts at:      0     372    3204 
   Size:        372    2832
 
PspOMI G'GGCC_C

Cuts at:      0    1507    3204 
   Size:       1507    1697
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1965    1997    3204 
   Size:       1965      32    1207

  Fragments arranged by size: 

                1965    1207      32
 
PstI C_TGCA'G

Cuts at:      0     149     461     629    1157    1365    1439    1925    2318 
   Size:        149     312     168     528     208      74     486     393

Cuts at:   2318    2762    3204 
   Size:        444     442

  Fragments arranged by size: 

                 528     486     444     442     393     312     208     168
                 149      74
 
PvuII CAG'CTG

Cuts at:      0      97    1152    2689    2933    3204 
   Size:         97    1055    1537     244     271

  Fragments arranged by size: 

                1537    1055     271     244      97
 
SacI G_AGCT'C

Cuts at:      0    1505    3204 
   Size:       1505    1699
 
SalI G'TCGA_C

Cuts at:      0     140    1354    3204 
   Size:        140    1214    1850

  Fragments arranged by size: 

                1850    1214     140
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1534    1667    1882    2326    3136    3204 
   Size:       1534     133     215     444     810      68

  Fragments arranged by size: 

                1534     810     444     215     133      68
 
SbfI CC_TGCA'GG

Cuts at:      0     461    3204 
   Size:        461    2743
 
SfcI C'TryA_G

Cuts at:      0     145     457     625    1153    1361    1435    1921    2314 
   Size:        145     312     168     528     208      74     486     393

Cuts at:   2314    2436    2758    3132    3204 
   Size:        122     322     374      72

  Fragments arranged by size: 

                 528     486     393     374     322     312     208     168
                 145     122      74      72
 
SmlI C'TyrA_G

Cuts at:      0      21     433     442    2968    3118    3204 
   Size:         21     412       9    2526     150      86

  Fragments arranged by size: 

                2526     412     150      86      21       9
 
SspI AAT'ATT

Cuts at:      0    1326    3204 
   Size:       1326    1878
 
StuI AGG'CCT

Cuts at:      0    1335    3204 
   Size:       1335    1869
 
StyI C'CwwG_G

Cuts at:      0    1086    1748    1760    1976    1985    2838    3204 
   Size:       1086     662      12     216       9     853     366

  Fragments arranged by size: 

                1086     853     662     366     216      12       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1030    2301    3204 
   Size:       1030    1271     903

  Fragments arranged by size: 

                1271    1030     903
 
TatI w'GTAC_w

Cuts at:      0    1925    3204 
   Size:       1925    1279
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     943     975    1901    1925    1933    1957    2357    2389 
   Size:        943      32     926      24       8      24     400      32

Cuts at:   2389    3204 
   Size:        815

  Fragments arranged by size: 

                 943     926     815     400      32      32      24      24
                   8
 
Tth111I GACn'n_nGTC

Cuts at:      0     659    2538    3204 
   Size:        659    1879     666

  Fragments arranged by size: 

                1879     666     659
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1572    2991    3204 
   Size:       1572    1419     213

  Fragments arranged by size: 

                1572    1419     213
 
XhoI C'TCGA_G

Cuts at:      0      21    3204 
   Size:         21    3183
 
XmnI GAAnn'nnTTC

Cuts at:      0     551    2585    3204 
   Size:        551    2034     619

  Fragments arranged by size: 

                2034     619     551
 
ZraI GAC'GTC

Cuts at:      0    2556    3204 
   Size:       2556     648
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI   AflIII     AhdI     AleI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI     BaeI    BamHI
     BanI    BanII     BbsI    BbvCI     BclI     BglI Bme1580I    BmgBI
     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrFI    BssSI    BstBI   BstEII
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     ClaI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
    EcoRV     FalI    HaeII    Hin4I   HincII     HpaI     MmeI     MscI
     MslI   MspA1I     NaeI     NcoI   NgoMIV     NotI     NspI     PciI
    PflMI     PfoI    PpuMI   PspOMI     PsrI     PstI    PvuII     SacI
     SalI     SapI     SbfI     SfcI     SmlI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII     AgeI     AlfI     AscI     AseI    AsiSI
    AvrII     BbeI     BcgI    BciVI    BfrBI    BglII     BlpI     BmtI
     BplI    BsaAI    BsiWI    BspHI    BsrDI    BsrGI   BssHII   BstAPI
  BstZ17I     BtsI    CspCI   DraIII    EcoNI     FseI     FspI    FspAI
  HindIII     KasI     KpnI     MfeI     MluI     NarI     NdeI     NheI
     NruI     NsiI     PacI     PasI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PvuI    RsrII    SacII    SanDI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SwaI     XbaI     XmaI
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