(Linear) (Six-base) MAPSORT of: KIEE4263.seq Check: 5866 from: 1 to: 3204 KIEE4263.orf With 182 enzymes: SgfI * July 3, 2012 18:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 3112 3204 Size: 3112 92 AatII G_ACGT'C Cuts at: 0 2558 3204 Size: 2558 646 AccI GT'mk_AC Cuts at: 0 141 1355 2822 3204 Size: 141 1214 1467 382 Fragments arranged by size: 1467 1214 382 141 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1577 2938 3204 Size: 1577 1361 266 Fragments arranged by size: 1577 1361 266 AfeI AGC'GCT Cuts at: 0 2503 3204 Size: 2503 701 AflIII A'CryG_T Cuts at: 0 1935 3204 Size: 1935 1269 AhdI GACnn_n'nnGTC Cuts at: 0 198 3204 Size: 198 3006 AleI CACnn'nnGTG Cuts at: 0 575 3204 Size: 575 2629 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2458 2490 3204 Size: 2458 32 714 Fragments arranged by size: 2458 714 32 AlwNI CAG_nnn'CTG Cuts at: 0 1023 3204 Size: 1023 2181 ApaI G_GGCC'C Cuts at: 0 1511 3204 Size: 1511 1693 ApaLI G'TGCA_C Cuts at: 0 215 3204 Size: 215 2989 ApoI r'AATT_y Cuts at: 0 527 856 1701 3204 Size: 527 329 845 1503 Fragments arranged by size: 1503 845 527 329 AvaI C'yCGr_G Cuts at: 0 21 1504 1834 2152 2725 3204 Size: 21 1483 330 318 573 479 Fragments arranged by size: 1483 573 479 330 318 21 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 408 441 3204 Size: 408 33 2763 Fragments arranged by size: 2763 408 33 BamHI G'GATC_C Cuts at: 0 2732 3204 Size: 2732 472 BanI G'GyrC_C Cuts at: 0 1170 1610 2417 3204 Size: 1170 440 807 787 Fragments arranged by size: 1170 807 787 440 BanII G_rGCy'C Cuts at: 0 48 961 1505 1511 3204 Size: 48 913 544 6 1693 Fragments arranged by size: 1693 913 544 48 6 BbsI GAAGACnn'nnnn_ Cuts at: 0 544 2424 2605 3204 Size: 544 1880 181 599 Fragments arranged by size: 1880 599 544 181 BbvCI CC'TCA_GC Cuts at: 0 964 3204 Size: 964 2240 BclI T'GATC_A Cuts at: 0 1139 2175 3204 Size: 1139 1036 1029 Fragments arranged by size: 1139 1036 1029 BglI GCCn_nnn'nGGC Cuts at: 0 52 3204 Size: 52 3152 Bme1580I G_kGCm'C Cuts at: 0 219 1511 1911 2420 3204 Size: 219 1292 400 509 784 Fragments arranged by size: 1292 784 509 400 219 BmgBI CAC'GTC Cuts at: 0 2379 3204 Size: 2379 825 BmrI ACTGGGnnnn_n' Cuts at: 0 1267 2011 3204 Size: 1267 744 1193 Fragments arranged by size: 1267 1193 744 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1240 2092 2612 2922 3204 Size: 1240 852 520 310 282 Fragments arranged by size: 1240 852 520 310 282 Bpu10I CC'TnA_GC Cuts at: 0 310 815 964 1204 2092 2401 3204 Size: 310 505 149 240 888 309 803 Fragments arranged by size: 888 803 505 310 309 240 149 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 418 427 2953 3103 3204 Size: 418 9 2526 150 101 Fragments arranged by size: 2526 418 150 101 9 BsaI GGTCTCn'nnnn_ Cuts at: 0 869 1456 1750 3204 Size: 869 587 294 1454 Fragments arranged by size: 1454 869 587 294 BsaBI GATnn'nnATC Cuts at: 0 1183 3204 Size: 1183 2021 BsaHI Gr'CG_yC Cuts at: 0 2555 3204 Size: 2555 649 BsaWI w'CCGG_w Cuts at: 0 119 1181 1319 2895 3204 Size: 119 1062 138 1576 309 Fragments arranged by size: 1576 1062 309 138 119 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 943 973 2476 2506 2933 2963 3204 Size: 943 30 1503 30 427 30 241 Fragments arranged by size: 1503 943 427 241 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 997 1000 1680 3087 3204 Size: 997 3 680 1407 117 Fragments arranged by size: 1407 997 680 117 3 BseYI C'CCAG_C Cuts at: 0 2105 2232 2343 3204 Size: 2105 127 111 861 Fragments arranged by size: 2105 861 127 111 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 94 710 3204 Size: 94 616 2494 Fragments arranged by size: 2494 616 94 BsiEI CG_ry'CG Cuts at: 0 754 3084 3204 Size: 754 2330 120 Fragments arranged by size: 2330 754 120 BsiHKAI G_wGCw'C Cuts at: 0 219 1271 1505 1877 3204 Size: 219 1052 234 372 1327 Fragments arranged by size: 1327 1052 372 234 219 BsmI GAATG_Cn' Cuts at: 0 1543 2666 3204 Size: 1543 1123 538 Fragments arranged by size: 1543 1123 538 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2770 3204 Size: 2770 434 Bsp1286I G_dGCh'C Cuts at: 0 48 219 961 1271 1505 1511 1877 1911 Size: 48 171 742 310 234 6 366 34 Cuts at: 1911 2420 3204 Size: 509 784 Fragments arranged by size: 784 742 509 366 310 234 171 48 34 6 BspEI T'CCGG_A Cuts at: 0 119 1181 1319 3204 Size: 119 1062 138 1885 Fragments arranged by size: 1885 1062 138 119 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1478 2321 3112 3122 3204 Size: 1478 843 791 10 82 Fragments arranged by size: 1478 843 791 82 10 BsrBI CCG'CTC Cuts at: 0 90 1809 2949 3204 Size: 90 1719 1140 255 Fragments arranged by size: 1719 1140 255 90 BsrFI r'CCGG_y Cuts at: 0 1817 2057 3204 Size: 1817 240 1147 Fragments arranged by size: 1817 1147 240 BssSI C'ACGA_G Cuts at: 0 56 3204 Size: 56 3148 BstBI TT'CG_AA Cuts at: 0 1971 2658 3204 Size: 1971 687 546 Fragments arranged by size: 1971 687 546 BstEII G'GTnAC_C Cuts at: 0 1980 3204 Size: 1980 1224 BstXI CCAn_nnnn'nTGG Cuts at: 0 2070 2413 3204 Size: 2070 343 791 Fragments arranged by size: 2070 791 343 BstYI r'GATC_y Cuts at: 0 1184 1857 2732 3204 Size: 1184 673 875 472 Fragments arranged by size: 1184 875 673 472 Bsu36I CC'TnA_GG Cuts at: 0 338 3204 Size: 338 2866 BtgI C'CryG_G Cuts at: 0 642 1512 1782 1976 2838 3204 Size: 642 870 270 194 862 366 Fragments arranged by size: 870 862 642 366 270 194 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 577 3204 Size: 577 2627 ClaI AT'CG_AT Cuts at: 0 38 885 3204 Size: 38 847 2319 Fragments arranged by size: 2319 847 38 DraI TTT'AAA Cuts at: 0 183 2807 3204 Size: 183 2624 397 Fragments arranged by size: 2624 397 183 DrdI GACnn_nn'nnGTC Cuts at: 0 2554 3204 Size: 2554 650 EaeI y'GGCC_r Cuts at: 0 751 1577 3081 3204 Size: 751 826 1504 123 Fragments arranged by size: 1504 826 751 123 EagI C'GGCC_G Cuts at: 0 751 3081 3204 Size: 751 2330 123 Fragments arranged by size: 2330 751 123 EarI CTCTTCn'nnn_ Cuts at: 0 1273 1534 1553 1667 1882 2201 2326 3136 Size: 1273 261 19 114 215 319 125 810 Cuts at: 3136 3204 Size: 68 Fragments arranged by size: 1273 810 319 261 215 125 114 68 19 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2044 3204 Size: 2044 1160 EcoICRI GAG'CTC Cuts at: 0 1503 3204 Size: 1503 1701 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1240 1577 2092 2612 2922 2938 3204 Size: 1240 337 515 520 310 16 266 Fragments arranged by size: 1240 520 515 337 310 266 16 EcoO109I rG'GnC_Cy Cuts at: 0 372 1508 3204 Size: 372 1136 1696 Fragments arranged by size: 1696 1136 372 EcoRI G'AATT_C Cuts at: 0 1701 3204 Size: 1701 1503 EcoRV GAT'ATC Cuts at: 0 156 3204 Size: 156 3048 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 85 1548 1590 2217 3204 Size: 85 1463 42 627 987 Fragments arranged by size: 1463 987 627 85 42 HaeII r_GCGC'y Cuts at: 0 822 1827 2505 3204 Size: 822 1005 678 699 Fragments arranged by size: 1005 822 699 678 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 184 216 2843 2875 2933 2965 3204 Size: 184 32 2627 32 58 32 239 Fragments arranged by size: 2627 239 184 58 32 32 32 HincII GTy'rAC Cuts at: 0 142 638 1356 3204 Size: 142 496 718 1848 Fragments arranged by size: 1848 718 496 142 HpaI GTT'AAC Cuts at: 0 638 3204 Size: 638 2566 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 115 1085 2909 2984 3204 Size: 115 970 1824 75 220 Fragments arranged by size: 1824 970 220 115 75 MscI TGG'CCA Cuts at: 0 1579 3204 Size: 1579 1625 MslI CAynn'nnrTG Cuts at: 0 575 775 1585 2131 3178 3204 Size: 575 200 810 546 1047 26 Fragments arranged by size: 1047 810 575 546 200 26 MspA1I CmG'CkG Cuts at: 0 97 1152 2689 2933 2990 3204 Size: 97 1055 1537 244 57 214 Fragments arranged by size: 1537 1055 244 214 97 57 NaeI GCC'GGC Cuts at: 0 1819 3204 Size: 1819 1385 NcoI C'CATG_G Cuts at: 0 1976 2838 3204 Size: 1976 862 366 Fragments arranged by size: 1976 862 366 NgoMIV G'CCGG_C Cuts at: 0 1817 3204 Size: 1817 1387 NotI GC'GGCC_GC Cuts at: 0 3081 3204 Size: 3081 123 NspI r_CATG'y Cuts at: 0 849 1939 2038 3204 Size: 849 1090 99 1166 Fragments arranged by size: 1166 1090 849 99 PciI A'CATG_T Cuts at: 0 1935 3204 Size: 1935 1269 PflMI CCAn_nnn'nTGG Cuts at: 0 1107 1484 2646 3204 Size: 1107 377 1162 558 Fragments arranged by size: 1162 1107 558 377 PfoI T'CCnGG_A Cuts at: 0 2062 2589 3204 Size: 2062 527 615 Fragments arranged by size: 2062 615 527 PpuMI rG'GwC_Cy Cuts at: 0 372 3204 Size: 372 2832 PspOMI G'GGCC_C Cuts at: 0 1507 3204 Size: 1507 1697 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1965 1997 3204 Size: 1965 32 1207 Fragments arranged by size: 1965 1207 32 PstI C_TGCA'G Cuts at: 0 149 461 629 1157 1365 1439 1925 2318 Size: 149 312 168 528 208 74 486 393 Cuts at: 2318 2762 3204 Size: 444 442 Fragments arranged by size: 528 486 444 442 393 312 208 168 149 74 PvuII CAG'CTG Cuts at: 0 97 1152 2689 2933 3204 Size: 97 1055 1537 244 271 Fragments arranged by size: 1537 1055 271 244 97 SacI G_AGCT'C Cuts at: 0 1505 3204 Size: 1505 1699 SalI G'TCGA_C Cuts at: 0 140 1354 3204 Size: 140 1214 1850 Fragments arranged by size: 1850 1214 140 SapI GCTCTTCn'nnn_ Cuts at: 0 1534 1667 1882 2326 3136 3204 Size: 1534 133 215 444 810 68 Fragments arranged by size: 1534 810 444 215 133 68 SbfI CC_TGCA'GG Cuts at: 0 461 3204 Size: 461 2743 SfcI C'TryA_G Cuts at: 0 145 457 625 1153 1361 1435 1921 2314 Size: 145 312 168 528 208 74 486 393 Cuts at: 2314 2436 2758 3132 3204 Size: 122 322 374 72 Fragments arranged by size: 528 486 393 374 322 312 208 168 145 122 74 72 SmlI C'TyrA_G Cuts at: 0 21 433 442 2968 3118 3204 Size: 21 412 9 2526 150 86 Fragments arranged by size: 2526 412 150 86 21 9 SspI AAT'ATT Cuts at: 0 1326 3204 Size: 1326 1878 StuI AGG'CCT Cuts at: 0 1335 3204 Size: 1335 1869 StyI C'CwwG_G Cuts at: 0 1086 1748 1760 1976 1985 2838 3204 Size: 1086 662 12 216 9 853 366 Fragments arranged by size: 1086 853 662 366 216 12 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1030 2301 3204 Size: 1030 1271 903 Fragments arranged by size: 1271 1030 903 TatI w'GTAC_w Cuts at: 0 1925 3204 Size: 1925 1279 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 943 975 1901 1925 1933 1957 2357 2389 Size: 943 32 926 24 8 24 400 32 Cuts at: 2389 3204 Size: 815 Fragments arranged by size: 943 926 815 400 32 32 24 24 8 Tth111I GACn'n_nGTC Cuts at: 0 659 2538 3204 Size: 659 1879 666 Fragments arranged by size: 1879 666 659 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1572 2991 3204 Size: 1572 1419 213 Fragments arranged by size: 1572 1419 213 XhoI C'TCGA_G Cuts at: 0 21 3204 Size: 21 3183 XmnI GAAnn'nnTTC Cuts at: 0 551 2585 3204 Size: 551 2034 619 Fragments arranged by size: 2034 619 551 ZraI GAC'GTC Cuts at: 0 2556 3204 Size: 2556 648 Enzymes that do cut: AarI AatII AccI AcuI AfeI AflIII AhdI AleI AloI AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI BanI BanII BbsI BbvCI BclI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HpaI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NotI NspI PciI PflMI PfoI PpuMI PspOMI PsrI PstI PvuII SacI SalI SapI SbfI SfcI SmlI SspI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmnI ZraI Enzymes that do not cut: Acc65I AclI AflII AgeI AlfI AscI AseI AsiSI AvrII BbeI BcgI BciVI BfrBI BglII BlpI BmtI BplI BsaAI BsiWI BspHI BsrDI BsrGI BssHII BstAPI BstZ17I BtsI CspCI DraIII EcoNI FseI FspI FspAI HindIII KasI KpnI MfeI MluI NarI NdeI NheI NruI NsiI PacI PasI PmeI PmlI PpiI PshAI PsiI PspXI PvuI RsrII SacII SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI XbaI XmaI