################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## Acc65I AccIII AclI AfaI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AviII BbrPI BbvCI BclI BfrI BglII BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I CelII Cfr10I ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI MamI MfeI MluI MroI MseI MunI NaeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SalI ScaI SfuI SgrAI SmlI SnaBI SpeI SphI SspBI SspI SwaI Tru9I XbaI XhoI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 88 GTCTAC GT^[AC][GT]AC AflIII 923 ACATGT A^C[GA][CT]GT Asp700I 1887 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 2427 GAGCAC G[AT]GC[AT]^C AvrII 1221 CCTAGG C^CTAGG BalI 494 TGGCCA TGG^CCA BbsI 1405 GTCTTC GAAGAC(2/6) BlnI 1221 CCTAGG C^CTAGG BpuAI 1405 GTCTTC GAAGAC(2/6) BsaI 913 GAGACC GGTCTC(1/5) BsgI 649 CTGCAC GTGCAG(16/14) BsiHKAI 2427 GAGCAC G[AT]GC[AT]^C BspLU11I 923 ACATGT A^CATGT BsrBI 328 GAGCGG CCGCTC(-3/-3) BstAPI 1518 GCATGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 2295 GGTGACC G^GT[ACGT]ACC BstPI 2295 GGTGACC G^GT[ACGT]ACC DraIII 997 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1143 GACTATTCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 171 CCTCCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2295 GGTGACC G^GT[ACGT]ACC EcoRI 1162 GAATTC G^AATTC HincII 2152 GTCAAC GT[CT]^[GA]AC HindII 2152 GTCAAC GT[CT]^[GA]AC KspI 34 CCGCGG CCGC^GG MluNI 494 TGGCCA TGG^CCA MscI 494 TGGCCA TGG^CCA MslI 208 CACCCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeI 1381 CATATG CA^TATG SacII 34 CCGCGG CCGC^GG SapI 681 GAAGAGC GCTCTTC(1/4) SexAI 998 ACCAGGT A^CC[AT]GGT SfiI 1985 GGCCTGAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 947 CCCGGG CCC^GGG StuI 1644 AGGCCT AGG^CCT XmaCI 947 CCCGGG C^CCGGG XmaI 947 CCCGGG C^CCGGG XmnI 1887 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AatII 85 GACGTC GACGT^C 586 GACGTC AspI 103 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC 483 GACAGCGTC BbeI 1012 GGCGCC GGCGC^C 1387 GGCGCC BcgI 540 CGAGGTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 825 GCAGCTCCCTCG BciVI 1533 GGATAC GTATCC(6/5) 2283 GGATAC BglI 1986 GCCTGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2176 GCCACACTGGC BsmI 381 GCATTC GAATGC(1/-1) 2369 GCATTC KasI 1012 GGCGCC G^GCGCC 1387 GGCGCC NarI 1012 GGCGCC GG^CGCC 1387 GGCGCC NcoI 1193 CCATGG C^CATGG 2259 CCATGG NspI 923 ACATGT [GA]CATG^[CT] 2156 ACATGC PflFI 103 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC 483 GACAGCGTC PvuII 850 CAGCTG CAG^CTG 1916 CAGCTG SbfI 291 CCTGCAGG CCTGCA^GG 2229 CCTGCAGG SfoI 1012 GGCGCC GGC^GCC 1387 GGCGCC Sse8387I 291 CCTGCAGG CCTGCA^GG 2229 CCTGCAGG Tth111I 103 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC 483 GACAGCGTC ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AccII AciI AcsI AcyI AluI AlwI AlwNI AocI ApaI ApoI AvaI AvaII BamHI BanI BanII BbvI BcnI BfaI BmyI BpmI BsaHI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII