(Circular) (Six-base) MAPSORT of: pFN21AB5767.seq Check: 7810 from: 1 to: 7141 pFN21AB5767 7141 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2391 2661 3958 4087 2391 Size: 270 1297 129 5445 Fragments arranged by size: 5445 1297 270 129 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2094 2595 4951 278 Size: 53 83 186 936 558 501 2356 2468 Fragments arranged by size: 2468 2356 936 558 501 186 83 53 AccI GT'mk_AC Cuts at: 1937 2094 4484 1937 Size: 157 2390 4594 Fragments arranged by size: 4594 2390 157 Acc65I G'GTAC_C Cuts at: 4462 4462 Size: 7141 AclI AA'CG_TT Cuts at: 1563 5268 5641 1563 Size: 3705 373 3063 Fragments arranged by size: 3705 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2387 3911 5204 6588 6856 1632 Size: 755 1524 1293 1384 268 1917 Fragments arranged by size: 1917 1524 1384 1293 755 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6944 AflIII A'CryG_T Cuts at: 1203 2928 6040 7017 1203 Size: 1725 3112 977 1327 Fragments arranged by size: 3112 1725 1327 977 AgeI A'CCGG_T Cuts at: 5972 5972 Size: 7141 AhdI GACnn_n'nnGTC Cuts at: 5870 5870 Size: 7141 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7141 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7141 AlwNI CAG_nnn'CTG Cuts at: 2495 2860 3178 3481 4884 6456 2495 Size: 365 318 303 1403 1572 3180 Fragments arranged by size: 3180 1572 1403 365 318 303 ApaI G_GGCC'C Cuts at: 2325 2602 2671 2845 3220 3637 4002 4351 2325 Size: 277 69 174 375 417 365 349 5115 Fragments arranged by size: 5115 417 375 365 349 277 174 69 ApaLI G'TGCA_C Cuts at: 5198 6354 5198 Size: 1156 5985 ApoI r'AATT_y Cuts at: 1493 2737 3011 3167 4450 4676 4828 6937 1493 Size: 1244 274 156 1283 226 152 2109 1697 Fragments arranged by size: 2109 1697 1283 1244 274 226 156 152 AseI AT'TA_AT Cuts at: 160 1032 5695 160 Size: 872 4663 1606 Fragments arranged by size: 4663 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7141 AvaI C'yCGr_G Cuts at: 1870 1943 2543 2838 2952 3468 4466 1870 Size: 73 600 295 114 516 998 4545 Fragments arranged by size: 4545 998 600 516 295 114 73 AvrII C'CTAG_G Cuts at: 3226 3226 Size: 7141 BamHI G'GATC_C Cuts at: 3337 3432 4077 4471 4838 3337 Size: 95 645 394 367 5640 Fragments arranged by size: 5640 645 394 367 95 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3017 3392 3809 4189 4462 Size: 325 217 627 1230 375 417 380 273 Cuts at: 4462 5917 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1230 627 417 380 375 325 273 217 BanII G_rGCy'C Cuts at: 1391 2294 2325 2376 2602 2671 2845 3220 3366 Size: 903 31 51 226 69 174 375 146 Cuts at: 3366 3637 4002 4176 4351 4460 1391 Size: 271 365 174 175 109 4072 Fragments arranged by size: 4072 903 375 365 271 226 175 174 174 146 109 69 51 31 BbeI G_GCGC'C Cuts at: 3021 3396 3021 Size: 375 6766 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3403 928 Size: 971 1504 4666 Fragments arranged by size: 4666 1504 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2534 2568 2819 2853 5332 5366 1903 Size: 34 597 34 251 34 2479 34 3678 Fragments arranged by size: 3678 2479 597 251 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3532 4282 5033 6243 3532 Size: 750 751 1210 4430 Fragments arranged by size: 4430 1210 751 750 BclI T'GATC_A Cuts at: 1548 1548 Size: 7141 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3997 4187 5752 Size: 107 122 71 631 605 2325 190 1565 Cuts at: 5752 136 Size: 1525 Fragments arranged by size: 2325 1565 1525 631 605 190 122 107 71 BglII A'GATC_T Cuts at: 7136 7136 Size: 7141 BlpI GC'TnA_GC Cuts at: 4558 4558 Size: 7141 Bme1580I G_kGCm'C Cuts at: 1790 2325 2602 2671 2845 3220 3637 4002 4351 Size: 535 277 69 174 375 417 365 349 Cuts at: 4351 5202 6358 1790 Size: 851 1156 2573 Fragments arranged by size: 2573 1156 851 535 417 375 365 349 277 174 69 BmgBI CAC'GTC Cuts at: 1318 2118 1318 Size: 800 6341 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2328 3089 4872 Size: 416 53 83 399 24 911 761 1783 Cuts at: 4872 5830 442 Size: 958 1753 Fragments arranged by size: 1783 1753 958 911 761 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7141 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3199 3199 Size: 7141 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2162 2348 2426 2936 3152 3234 3566 3886 Size: 923 186 78 510 216 82 332 320 Cuts at: 3886 3953 4055 4279 4388 5801 1239 Size: 67 102 224 109 1413 2579 Fragments arranged by size: 2579 1413 923 510 332 320 224 216 186 109 102 82 78 67 Bpu10I CC'TnA_GC Cuts at: 4194 4194 Size: 7141 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5266 6131 6429 5266 Size: 865 298 5978 Fragments arranged by size: 5978 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2912 5804 882 Size: 617 1413 2892 2219 Fragments arranged by size: 2892 2219 1413 617 BsaAI yAC'GTr Cuts at: 493 1204 4932 493 Size: 711 3728 2702 Fragments arranged by size: 3728 2702 711 BsaBI GATnn'nnATC Cuts at: 7135 7135 Size: 7141 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2091 2592 3018 3393 Size: 53 83 186 936 558 501 426 375 Cuts at: 3393 4049 4948 5330 5965 275 Size: 656 899 382 635 1451 Fragments arranged by size: 1451 936 899 656 635 558 501 426 382 375 186 83 53 BsaWI w'CCGG_w Cuts at: 5574 5972 6246 6393 6804 5574 Size: 398 274 147 411 5911 Fragments arranged by size: 5911 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2156 2186 2981 3011 3053 Size: 30 165 30 753 30 795 30 42 Cuts at: 3053 3083 3232 3251 3262 3281 1178 Size: 30 149 19 11 19 5038 Fragments arranged by size: 5038 795 753 165 149 42 30 30 30 30 30 19 19 11 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2345 2423 2521 2837 3272 1186 Size: 1159 78 98 316 435 5055 Fragments arranged by size: 5055 1159 435 316 98 78 BseYI C'CCAG_C Cuts at: 2623 2968 3714 3914 3919 3923 4092 6344 6840 Size: 345 746 200 5 4 169 2252 496 Cuts at: 6840 2623 Size: 2924 Fragments arranged by size: 2924 2252 746 496 345 200 169 5 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2639 1163 Size: 573 903 5665 Fragments arranged by size: 5665 903 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4591 5352 6380 1540 Size: 275 186 2590 761 1028 2301 Fragments arranged by size: 2590 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4436 5202 5287 6358 4436 Size: 766 85 1071 5219 Fragments arranged by size: 5219 1071 766 85 BsmI GAATG_Cn' Cuts at: 2386 4374 4652 4745 2386 Size: 1988 278 93 4782 Fragments arranged by size: 4782 1988 278 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2294 2325 2376 2602 2671 2845 3220 Size: 399 504 31 51 226 69 174 375 Cuts at: 3220 3366 3637 4002 4176 4351 4436 4460 5202 Size: 146 271 365 174 175 85 24 742 Cuts at: 5202 5287 6358 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 504 399 375 365 271 226 175 174 174 146 85 85 69 51 31 24 BspEI T'CCGG_A Cuts at: 6804 6804 Size: 7141 BspHI T'CATG_A Cuts at: 5030 6760 5030 Size: 1730 5411 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2391 2661 3528 3958 4087 4496 844 Size: 1547 270 867 430 129 409 3489 Fragments arranged by size: 3489 1547 867 430 409 270 129 BsrBI CCG'CTC Cuts at: 1394 1823 2335 5028 1394 Size: 429 512 2693 3507 Fragments arranged by size: 3507 2693 512 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5636 5810 65 Size: 1183 4388 174 1396 Fragments arranged by size: 4388 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5785 5972 1700 Size: 110 3975 187 2869 Fragments arranged by size: 3975 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7141 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7141 BssSI C'ACGA_G Cuts at: 5201 6213 5201 Size: 1012 6129 BstAPI GCAn_nnn'nTGC Cuts at: 794 3529 794 Size: 2735 4406 BstBI TT'CG_AA Cuts at: 5945 5945 Size: 7141 BstEII G'GTnAC_C Cuts at: 1184 4300 1184 Size: 3116 4025 BstXI CCAn_nnnn'nTGG Cuts at: 2224 2318 2623 2224 Size: 94 305 6742 Fragments arranged by size: 6742 305 94 BstYI r'GATC_y Cuts at: 1972 2265 2400 2991 3337 3432 4077 4329 4471 Size: 293 135 591 346 95 645 252 142 Cuts at: 4471 4838 5223 5240 6692 6778 6790 7136 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 645 591 385 367 346 346 293 252 142 135 95 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2246 2901 3993 4071 2246 Size: 655 1092 78 5316 Fragments arranged by size: 5316 1092 655 78 BtgI C'CryG_G Cuts at: 513 1065 2004 2039 3056 3198 3306 3358 4176 Size: 552 939 35 1017 142 108 52 818 Cuts at: 4176 4264 513 Size: 88 3390 Fragments arranged by size: 3390 1017 939 818 552 142 108 88 52 35 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3548 487 Size: 683 56 2322 4080 Fragments arranged by size: 4080 2322 683 56 BtsI GCAGTG_nn' Cuts at: 2610 3296 3785 3863 4657 5451 5471 2610 Size: 686 489 78 794 794 20 4280 Fragments arranged by size: 4280 794 794 686 489 78 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7141 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7106 DraI TTT'AAA Cuts at: 4445 4445 Size: 7141 DraIII CAC_nnn'GTG Cuts at: 3007 3007 Size: 7141 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3154 6148 809 Size: 507 1838 2994 1802 Fragments arranged by size: 2994 1838 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2127 2499 3825 Size: 54 1425 50 275 18 297 372 1326 Cuts at: 3825 4179 4588 5477 7106 8 Size: 354 409 889 1629 43 Fragments arranged by size: 1629 1425 1326 889 409 372 354 297 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4588 1537 Size: 275 2776 4090 Fragments arranged by size: 4090 2776 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2681 2777 3060 3084 5071 1353 Size: 1328 96 283 24 1987 3423 Fragments arranged by size: 3423 1987 1328 283 96 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5728 6102 6248 387 Size: 1064 138 4139 374 146 1280 Fragments arranged by size: 4139 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2162 2348 2387 2426 2936 3152 3234 Size: 393 530 186 39 39 510 216 82 Cuts at: 3234 3566 3886 3911 3953 4055 4279 4388 5204 Size: 332 320 25 42 102 224 109 816 Cuts at: 5204 5801 6588 6856 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 816 787 597 530 510 393 332 320 268 224 216 186 109 102 82 42 39 39 25 EcoNI CCTnn'n_nnAGG Cuts at: 2180 2180 Size: 7141 EcoO109I rG'GnC_Cy Cuts at: 2035 2079 2322 2599 2667 2668 2842 2889 2979 Size: 44 243 277 68 1 174 47 90 Cuts at: 2979 3127 3217 3281 3989 3998 4347 2035 Size: 148 90 64 708 9 349 4829 Fragments arranged by size: 4829 708 349 277 243 174 148 90 90 68 64 47 44 9 1 EcoRI G'AATT_C Cuts at: 3167 4450 4828 3167 Size: 1283 378 5480 Fragments arranged by size: 5480 1283 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2710 2710 Size: 7141 FspI TGC'GCA Cuts at: 5647 5647 Size: 7141 HaeII r_GCGC'y Cuts at: 1678 2012 3021 3396 3736 6288 1678 Size: 334 1009 375 340 2552 2531 Fragments arranged by size: 2552 2531 1009 375 340 334 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2156 2188 2758 2790 3251 Size: 32 135 32 584 32 570 32 461 Cuts at: 3251 3283 3335 3367 3547 3579 4347 4379 5782 Size: 32 52 32 180 32 768 32 1403 Cuts at: 5782 5814 5856 5888 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1403 768 584 570 461 180 135 52 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4159 4485 4731 669 Size: 988 281 2221 326 246 3079 Fragments arranged by size: 3079 2221 988 326 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7141 HpaI GTT'AAC Cuts at: 4731 4731 Size: 7141 KasI G'GCGC_C Cuts at: 3017 3392 3017 Size: 375 6766 KpnI G_GTAC'C Cuts at: 4466 4466 Size: 7141 MfeI C'AATT_G Cuts at: 4740 4740 Size: 7141 MluI A'CGCG_T Cuts at: 7017 7017 Size: 7141 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6255 6439 1305 Size: 4950 184 2007 Fragments arranged by size: 4950 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2501 10 Size: 54 1425 343 669 4650 Fragments arranged by size: 4650 1425 669 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2217 5099 5458 5617 518 Size: 648 1051 2882 359 159 2042 Fragments arranged by size: 2882 2042 1051 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2041 2857 3331 3664 3871 3923 3940 4557 Size: 435 816 474 333 207 52 17 617 Cuts at: 4557 5234 6382 6627 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 816 677 617 474 435 333 245 207 52 17 NarI GG'CG_CC Cuts at: 3018 3393 3018 Size: 375 6766 NcoI C'CATG_G Cuts at: 513 1065 2004 3198 4264 513 Size: 552 939 1194 1066 3390 Fragments arranged by size: 3390 1194 1066 939 552 NdeI CA'TA_TG Cuts at: 387 3387 387 Size: 3000 4141 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7141 NotI GC'GGCC_GC Cuts at: 4588 4588 Size: 7141 NspI r_CATG'y Cuts at: 1132 1229 2932 4165 4499 6044 1132 Size: 97 1703 1233 334 1545 2229 Fragments arranged by size: 2229 1703 1545 1233 334 97 PasI CC'CwG_GG Cuts at: 1348 2156 2602 1348 Size: 808 446 5887 Fragments arranged by size: 5887 808 446 PciI A'CATG_T Cuts at: 2928 6040 2928 Size: 3112 4029 PflMI CCAn_nnn'nTGG Cuts at: 1108 3178 3312 3481 1108 Size: 2070 134 169 4768 Fragments arranged by size: 4768 2070 169 134 PfoI T'CCnGG_A Cuts at: 3120 3171 4365 3120 Size: 51 1194 5896 Fragments arranged by size: 5896 1194 51 PmeI GTTT'AAAC Cuts at: 4445 4445 Size: 7141 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5210 5242 6723 6755 5210 Size: 32 1481 32 5596 Fragments arranged by size: 5596 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2035 2079 2889 2979 2035 Size: 44 810 90 6197 Fragments arranged by size: 6197 810 90 44 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7141 PsiI TTA'TAA Cuts at: 4711 4711 Size: 7141 PspOMI G'GGCC_C Cuts at: 2321 2598 2667 2841 3216 3633 3998 4347 2321 Size: 277 69 174 375 417 365 349 5115 Fragments arranged by size: 5115 417 375 365 349 277 174 69 PstI C_TGCA'G Cuts at: 830 2034 2064 2301 2637 2811 3114 3858 3876 Size: 1204 30 237 336 174 303 744 18 Cuts at: 3876 4239 4493 830 Size: 363 254 3478 Fragments arranged by size: 3478 1204 744 363 336 303 254 237 174 30 18 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7141 PvuII CAG'CTG Cuts at: 2857 3923 2857 Size: 1066 6075 SacII CC_GC'GG Cuts at: 2042 2042 Size: 7141 SalI G'TCGA_C Cuts at: 1936 4483 1936 Size: 2547 4594 SanDI GG'GwC_CC Cuts at: 2889 2889 Size: 7141 SapI GCTCTTCn'nnn_ Cuts at: 2681 2681 Size: 7141 SbfI CC_TGCA'GG Cuts at: 2301 4239 4493 2301 Size: 1938 254 4949 Fragments arranged by size: 4949 1938 254 ScaI AGT'ACT Cuts at: 5389 7101 5389 Size: 1712 5429 SexAI A'CCwGG_T Cuts at: 3003 3003 Size: 7141 SfcI C'TryA_G Cuts at: 826 1045 2030 2060 2297 2633 2807 3110 3671 Size: 219 985 30 237 336 174 303 561 Cuts at: 3671 3854 3872 4235 4489 5624 6305 6496 826 Size: 183 18 363 254 1135 681 191 1471 Fragments arranged by size: 1471 1135 985 681 561 363 336 303 254 237 219 191 183 174 30 18 SfiI GGCCn_nnn'nGGCC Cuts at: 3997 3997 Size: 7141 SfoI GGC'GCC Cuts at: 3019 3394 3019 Size: 375 6766 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7141 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7141 SmaI CCC'GGG Cuts at: 1872 2954 4468 1872 Size: 1082 1514 4545 Fragments arranged by size: 4545 1514 1082 SmlI C'TyrA_G Cuts at: 820 1017 1943 5245 6146 6408 820 Size: 197 926 3302 901 262 1553 Fragments arranged by size: 3302 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4932 493 Size: 4439 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7141 SphI G_CATG'C Cuts at: 1132 4499 1132 Size: 3367 3774 SspI AAT'ATT Cuts at: 5 52 5065 5 Size: 47 5013 2081 Fragments arranged by size: 5013 2081 47 StuI AGG'CCT Cuts at: 1517 3651 1517 Size: 2134 5007 StyI C'CwwG_G Cuts at: 513 1065 2004 3198 3226 3378 3405 4264 4580 Size: 552 939 1194 28 152 27 859 316 Cuts at: 4580 513 Size: 3074 Fragments arranged by size: 3074 1194 939 859 552 316 152 28 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3106 3144 3728 5168 5185 5338 5523 Size: 1255 1526 38 584 1440 17 153 185 Cuts at: 5523 5968 325 Size: 445 1498 Fragments arranged by size: 1526 1498 1440 1255 584 445 185 153 38 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5387 7099 96 Size: 274 80 33 51 1443 3410 1712 138 Fragments arranged by size: 3410 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3053 3085 3232 3264 3739 3771 7101 7133 3053 Size: 32 147 32 475 32 3330 32 3061 Fragments arranged by size: 3330 3061 475 147 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2111 2491 1316 Size: 795 380 5966 Fragments arranged by size: 5966 795 380 XbaI T'CTAG_A Cuts at: 4477 4477 Size: 7141 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2323 3011 3385 3820 4183 4222 2323 Size: 688 374 435 363 39 5242 Fragments arranged by size: 5242 688 435 374 363 39 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7141 XmaI C'CCGG_G Cuts at: 1870 2952 4466 1870 Size: 1082 1514 4545 Fragments arranged by size: 4545 1514 1082 XmnI GAAnn'nnTTC Cuts at: 3896 5270 3896 Size: 1374 5767 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2092 2593 4949 276 Size: 53 83 186 936 558 501 2356 2468 Fragments arranged by size: 2468 2356 936 558 501 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SrfI SwaI