(Linear) (Six-base) MAPSORT of: KIBB5767.seq Check: 1772 from: 1 to: 2436 KIBB5767.orf With 182 enzymes: SgfI * July 3, 2012 16:05 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 386 656 1953 2082 2436 Size: 386 270 1297 129 354 Fragments arranged by size: 1297 386 354 270 129 AatII G_ACGT'C Cuts at: 0 89 590 2436 Size: 89 501 1846 Fragments arranged by size: 1846 501 89 AccI GT'mk_AC Cuts at: 0 89 2436 Size: 89 2347 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 382 1906 2436 Size: 382 1524 530 Fragments arranged by size: 1524 530 382 AflIII A'CryG_T Cuts at: 0 923 2436 Size: 923 1513 AlwNI CAG_nnn'CTG Cuts at: 0 490 855 1173 1476 2436 Size: 490 365 318 303 960 Fragments arranged by size: 960 490 365 318 303 ApaI G_GGCC'C Cuts at: 0 320 597 666 840 1215 1632 1997 2346 Size: 320 277 69 174 375 417 365 349 Cuts at: 2346 2436 Size: 90 Fragments arranged by size: 417 375 365 349 320 277 174 90 69 ApoI r'AATT_y Cuts at: 0 732 1006 1162 2436 Size: 732 274 156 1274 Fragments arranged by size: 1274 732 274 156 AvaI C'yCGr_G Cuts at: 0 538 833 947 1463 2436 Size: 538 295 114 516 973 Fragments arranged by size: 973 538 516 295 114 AvrII C'CTAG_G Cuts at: 0 1221 2436 Size: 1221 1215 BamHI G'GATC_C Cuts at: 0 1332 1427 2072 2436 Size: 1332 95 645 364 Fragments arranged by size: 1332 645 364 95 BanI G'GyrC_C Cuts at: 0 1012 1387 1804 2184 2436 Size: 1012 375 417 380 252 Fragments arranged by size: 1012 417 380 375 252 BanII G_rGCy'C Cuts at: 0 289 320 371 597 666 840 1215 1361 Size: 289 31 51 226 69 174 375 146 Cuts at: 1361 1632 1997 2171 2346 2436 Size: 271 365 174 175 90 Fragments arranged by size: 375 365 289 271 226 175 174 174 146 90 69 51 31 BbeI G_GCGC'C Cuts at: 0 1016 1391 2436 Size: 1016 375 1045 Fragments arranged by size: 1045 1016 375 BbsI GAAGACnn'nnnn_ Cuts at: 0 1398 2436 Size: 1398 1038 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 529 563 814 848 2436 Size: 529 34 251 34 1588 Fragments arranged by size: 1588 529 251 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1527 2277 2436 Size: 1527 750 159 Fragments arranged by size: 1527 750 159 BglI GCCn_nnn'nGGC Cuts at: 0 1992 2182 2436 Size: 1992 190 254 Fragments arranged by size: 1992 254 190 Bme1580I G_kGCm'C Cuts at: 0 320 597 666 840 1215 1632 1997 2346 Size: 320 277 69 174 375 417 365 349 Cuts at: 2346 2436 Size: 90 Fragments arranged by size: 417 375 365 349 320 277 174 90 69 BmgBI CAC'GTC Cuts at: 0 113 2436 Size: 113 2323 BmrI ACTGGGnnnn_n' Cuts at: 0 323 1084 2436 Size: 323 761 1352 Fragments arranged by size: 1352 761 323 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1194 2436 Size: 1194 1242 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 157 343 421 931 1147 1229 1561 1881 Size: 157 186 78 510 216 82 332 320 Cuts at: 1881 1948 2050 2274 2383 2436 Size: 67 102 224 109 53 Fragments arranged by size: 510 332 320 224 216 186 157 109 102 82 78 67 53 Bpu10I CC'TnA_GC Cuts at: 0 2189 2436 Size: 2189 247 BsaI GGTCTCn'nnnn_ Cuts at: 0 907 2436 Size: 907 1529 BsaHI Gr'CG_yC Cuts at: 0 86 587 1013 1388 2044 2436 Size: 86 501 426 375 656 392 Fragments arranged by size: 656 501 426 392 375 86 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 151 181 976 1006 1048 1078 1227 1246 Size: 151 30 795 30 42 30 149 19 Cuts at: 1246 1257 1276 2436 Size: 11 19 1160 Fragments arranged by size: 1160 795 151 149 42 30 30 30 19 19 11 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 340 418 516 832 1267 2436 Size: 340 78 98 316 435 1169 Fragments arranged by size: 1169 435 340 316 98 78 BseYI C'CCAG_C Cuts at: 0 618 963 1709 1909 1914 1918 2087 2436 Size: 618 345 746 200 5 4 169 349 Fragments arranged by size: 746 618 349 345 200 169 5 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 634 2436 Size: 634 1802 BsiHKAI G_wGCw'C Cuts at: 0 2431 2436 Size: 2431 5 BsmI GAATG_Cn' Cuts at: 0 381 2369 2436 Size: 381 1988 67 Fragments arranged by size: 1988 381 67 Bsp1286I G_dGCh'C Cuts at: 0 289 320 371 597 666 840 1215 1361 Size: 289 31 51 226 69 174 375 146 Cuts at: 1361 1632 1997 2171 2346 2431 2436 Size: 271 365 174 175 85 5 Fragments arranged by size: 375 365 289 271 226 175 174 174 146 85 69 51 31 5 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 386 656 1523 1953 2082 2436 Size: 386 270 867 430 129 354 Fragments arranged by size: 867 430 386 354 270 129 BsrBI CCG'CTC Cuts at: 0 330 2436 Size: 330 2106 BstAPI GCAn_nnn'nTGC Cuts at: 0 1524 2436 Size: 1524 912 BstEII G'GTnAC_C Cuts at: 0 2295 2436 Size: 2295 141 BstXI CCAn_nnnn'nTGG Cuts at: 0 219 313 618 2436 Size: 219 94 305 1818 Fragments arranged by size: 1818 305 219 94 BstYI r'GATC_y Cuts at: 0 260 395 986 1332 1427 2072 2324 2436 Size: 260 135 591 346 95 645 252 112 Fragments arranged by size: 645 591 346 260 252 135 112 95 Bsu36I CC'TnA_GG Cuts at: 0 241 896 1988 2066 2436 Size: 241 655 1092 78 370 Fragments arranged by size: 1092 655 370 241 78 BtgI C'CryG_G Cuts at: 0 34 1051 1193 1301 1353 2171 2259 2436 Size: 34 1017 142 108 52 818 88 177 Fragments arranged by size: 1017 818 177 142 108 88 52 34 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1543 2436 Size: 1543 893 BtsI GCAGTG_nn' Cuts at: 0 605 1291 1780 1858 2436 Size: 605 686 489 78 578 Fragments arranged by size: 686 605 578 489 78 DraIII CAC_nnn'GTG Cuts at: 0 1002 2436 Size: 1002 1434 DrdI GACnn_nn'nnGTC Cuts at: 0 1149 2436 Size: 1149 1287 EaeI y'GGCC_r Cuts at: 0 122 494 1820 2174 2436 Size: 122 372 1326 354 262 Fragments arranged by size: 1326 372 354 262 122 EarI CTCTTCn'nnn_ Cuts at: 0 676 772 1055 1079 2436 Size: 676 96 283 24 1357 Fragments arranged by size: 1357 676 283 96 24 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 157 343 382 421 931 1147 1229 1561 Size: 157 186 39 39 510 216 82 332 Cuts at: 1561 1881 1906 1948 2050 2274 2383 2436 Size: 320 25 42 102 224 109 53 Fragments arranged by size: 510 332 320 224 216 186 157 109 102 82 53 42 39 39 25 EcoNI CCTnn'n_nnAGG Cuts at: 0 175 2436 Size: 175 2261 EcoO109I rG'GnC_Cy Cuts at: 0 30 74 317 594 662 663 837 884 Size: 30 44 243 277 68 1 174 47 Cuts at: 884 974 1122 1212 1276 1984 1993 2342 2436 Size: 90 148 90 64 708 9 349 94 Fragments arranged by size: 708 349 277 243 174 148 94 90 90 68 64 47 44 30 9 1 EcoRI G'AATT_C Cuts at: 0 1162 2436 Size: 1162 1274 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 705 2436 Size: 705 1731 HaeII r_GCGC'y Cuts at: 0 7 1016 1391 1731 2436 Size: 7 1009 375 340 705 Fragments arranged by size: 1009 705 375 340 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 151 183 753 785 1246 1278 1330 1362 Size: 151 32 570 32 461 32 52 32 Cuts at: 1362 1542 1574 2342 2374 2436 Size: 180 32 768 32 62 Fragments arranged by size: 768 570 461 180 151 62 52 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 2154 2436 Size: 2154 282 KasI G'GCGC_C Cuts at: 0 1012 1387 2436 Size: 1012 375 1049 Fragments arranged by size: 1049 1012 375 MscI TGG'CCA Cuts at: 0 496 2436 Size: 496 1940 MslI CAynn'nnrTG Cuts at: 0 212 2436 Size: 212 2224 MspA1I CmG'CkG Cuts at: 0 36 852 1326 1659 1866 1918 1935 2436 Size: 36 816 474 333 207 52 17 501 Fragments arranged by size: 816 501 474 333 207 52 36 17 NarI GG'CG_CC Cuts at: 0 1013 1388 2436 Size: 1013 375 1048 Fragments arranged by size: 1048 1013 375 NcoI C'CATG_G Cuts at: 0 1193 2259 2436 Size: 1193 1066 177 Fragments arranged by size: 1193 1066 177 NdeI CA'TA_TG Cuts at: 0 1382 2436 Size: 1382 1054 NspI r_CATG'y Cuts at: 0 927 2160 2436 Size: 927 1233 276 Fragments arranged by size: 1233 927 276 PasI CC'CwG_GG Cuts at: 0 151 597 2436 Size: 151 446 1839 Fragments arranged by size: 1839 446 151 PciI A'CATG_T Cuts at: 0 923 2436 Size: 923 1513 PflMI CCAn_nnn'nTGG Cuts at: 0 1173 1307 1476 2436 Size: 1173 134 169 960 Fragments arranged by size: 1173 960 169 134 PfoI T'CCnGG_A Cuts at: 0 1115 1166 2360 2436 Size: 1115 51 1194 76 Fragments arranged by size: 1194 1115 76 51 PpuMI rG'GwC_Cy Cuts at: 0 30 74 884 974 2436 Size: 30 44 810 90 1462 Fragments arranged by size: 1462 810 90 44 30 PspOMI G'GGCC_C Cuts at: 0 316 593 662 836 1211 1628 1993 2342 Size: 316 277 69 174 375 417 365 349 Cuts at: 2342 2436 Size: 94 Fragments arranged by size: 417 375 365 349 316 277 174 94 69 PstI C_TGCA'G Cuts at: 0 29 59 296 632 806 1109 1853 1871 Size: 29 30 237 336 174 303 744 18 Cuts at: 1871 2234 2436 Size: 363 202 Fragments arranged by size: 744 363 336 303 237 202 174 30 29 18 PvuII CAG'CTG Cuts at: 0 852 1918 2436 Size: 852 1066 518 Fragments arranged by size: 1066 852 518 SacII CC_GC'GG Cuts at: 0 37 2436 Size: 37 2399 SanDI GG'GwC_CC Cuts at: 0 884 2436 Size: 884 1552 SapI GCTCTTCn'nnn_ Cuts at: 0 676 2436 Size: 676 1760 SbfI CC_TGCA'GG Cuts at: 0 296 2234 2436 Size: 296 1938 202 Fragments arranged by size: 1938 296 202 SexAI A'CCwGG_T Cuts at: 0 998 2436 Size: 998 1438 SfcI C'TryA_G Cuts at: 0 25 55 292 628 802 1105 1666 1849 Size: 25 30 237 336 174 303 561 183 Cuts at: 1849 1867 2230 2436 Size: 18 363 206 Fragments arranged by size: 561 363 336 303 237 206 183 174 30 25 18 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1992 2436 Size: 1992 444 SfoI GGC'GCC Cuts at: 0 1014 1389 2436 Size: 1014 375 1047 Fragments arranged by size: 1047 1014 375 SmaI CCC'GGG Cuts at: 0 949 2436 Size: 949 1487 StuI AGG'CCT Cuts at: 0 1646 2436 Size: 1646 790 StyI C'CwwG_G Cuts at: 0 1193 1221 1373 1400 2259 2436 Size: 1193 28 152 27 859 177 Fragments arranged by size: 1193 859 177 152 28 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1101 1139 1723 2436 Size: 1101 38 584 713 Fragments arranged by size: 1101 713 584 38 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1048 1080 1227 1259 1734 1766 2436 Size: 1048 32 147 32 475 32 670 Fragments arranged by size: 1048 670 475 147 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 106 486 2436 Size: 106 380 1950 Fragments arranged by size: 1950 380 106 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 318 1006 1380 1815 2178 2217 2436 Size: 318 688 374 435 363 39 219 Fragments arranged by size: 688 435 374 363 318 219 39 XmaI C'CCGG_G Cuts at: 0 947 2436 Size: 947 1489 XmnI GAAnn'nnTTC Cuts at: 0 1891 2436 Size: 1891 545 ZraI GAC'GTC Cuts at: 0 87 588 2436 Size: 87 501 1848 Fragments arranged by size: 1848 501 87 Enzymes that do cut: AarI AatII AccI AcuI AflIII AlwNI ApaI ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII KasI MscI MslI MspA1I NarI NcoI NdeI NspI PasI PciI PflMI PfoI PpuMI PspOMI PstI PvuII SacII SanDI SapI SbfI SexAI SfcI SfiI SfoI SmaI StuI StyI TaqII TstI Tth111I XcmI XmaI XmnI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI BaeI BbvCI BclI BfrBI BglII BlpI BmtI BpuEI BsaAI BsaBI BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstZ17I ClaI CspCI DraI EagI EciI EcoICRI EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI NaeI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI ScaI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI XbaI XhoI