Restriction map for
pFN21AB6538
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspI AvaI AvrII BamHI
BanI BbeI BbrPI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BsaAI BsaBI BsaHI BsaI
BsiEI BsiWI BsmAI BsmBI BsoBI
Bsp1407I BspDI BspHI BspLU11I BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FbaI FseI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MseI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI StuI SwaI Tru9I Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 113 TCCGGA T^CCGGA
AcsI 661 GAATTC [GA]^AATT[CT]
AhdI 382 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 222 GATCC GGATC(4/5)
ApaI 1131 GGGCCC GGGCC^C
ApoI 661 GAATTC [GA]^AATT[CT]
Asp700I 231 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 382 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 533 TGCGCA TGC^GCA
BbvCI 853 CCTCAGC CCTCAGC(-5/-2)
BlpI 1039 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1039 GCTGAGC GC^T[ACGT]AGC
BsaWI 113 TCCGGA [AT]^CCGG[AT]
BseAI 113 TCCGGA T^CCGGA
BsgI 482 CTGCAC GTGCAG(16/14)
BsmI 710 GCATTC GAATGC(1/-1)
BspEI 113 TCCGGA T^CCGGA
BspMI 206 ACCTGC ACCTGC(4/8)
BstXI 569 CCAAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 221 AGATCC [GA]^GATC[CT]
CelII 1039 GCTGAGC GC^T[ACGT]AGC
Eam1105I 382 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 166 CTCTTC CTCTTC(1/4)
EcoNI 594 CCTTGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 661 GAATTC G^AATTC
FspI 533 TGCGCA TGC^GCA
Ksp632I 166 CTCTTC CTCTTC(1/4)
MaeII 26 ACGT A^CGT
MflI 221 AGATCC [GA]^GATC[CT]
MroI 113 TCCGGA T^CCGGA
MslI 715 CATTTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 428 ACATGC [GA]CATG^[CT]
PflMI 816 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 76 GAGCTC GAGCT^C
SapI 165 GCTCTTC GCTCTTC(1/4)
SbfI 393 CCTGCAGG CCTGCA^GG
SmlI 655 CTCAAG C^T[CT][GA]AG
Sse8387I 393 CCTGCAGG CCTGCA^GG
TaqI 407 TCGA T^CGA
TfiI 702 GAATC G^A[AT]TC
Tsp509I 662 AATT ^AATT
TthHB8I 407 TCGA T^CGA
Van91I 816 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1136 CCAGCCCTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 221 AGATCC [GA]^GATC[CT]
XmnI 231 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 436 CGCG CG^CG
956 CGCG
AfaI 1049 GTAC GT^AC
1117 GTAC
AspHI 34 GTGCTC G[AT]GC[AT]^C
76 GAGCTC
BalI 638 TGGCCA TGG^CCA
785 TGGCCA
BsiHKAI 34 GTGCTC G[AT]GC[AT]^C
76 GAGCTC
BsrDI 337 CATTGC GCAATG(2/0)
944 GCAATG
BssSI 544 CACGAG CACGAG(-5/-1)
417 CTCGTG
BstF5I 528 CATCC GGATG(2/0)
1104 CATCC
BstUI 436 CGCG CG^CG
956 CGCG
DpnII 222 GATC ^GATC
921 GATC
FokI 528 CATCC GGATG(9/13)
1104 CATCC
HgaI 498 GACGC GACGC(5/10)
837 GCGTC
MboI 222 GATC ^GATC
921 GATC
MluNI 638 TGGCCA TGG^CCA
785 TGGCCA
MscI 638 TGGCCA TGG^CCA
785 TGGCCA
MspA1I 331 CAGCTG C[AC]G^C[GT]G
616 CAGCTG
MvnI 436 CGCG CG^CG
956 CGCG
NcoI 575 CCATGG C^CATGG
926 CCATGG
NdeII 222 GATC ^GATC
921 GATC
PpuMI 203 AGGACCT [GA]G^G[AT]CC[CT]
818 AGGACCT
PstI 394 CTGCAG CTGCA^G
619 CTGCAG
PvuII 331 CAGCTG CAG^CTG
616 CAGCTG
RsaI 1049 GTAC GT^AC
1117 GTAC
Sau3AI 222 GATC ^GATC
921 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AluI AlwNI AvaII BanII
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaJI BseRI BsiYI BslI
BsmFI Bsp1286I BsrBI BsrI BssKI
BstNI Cac8I CfoI Cfr13I DdeI
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboII MnlI MspI
MvaI MwoI NciI NlaIII NlaIV
PleI Sau96I ScrFI SfaNI SfcI
StyI TseI Tsp45I TspRI