Restriction map for pFN21AB6538
 (Circular) (Six-base) MAPSORT of: pFN21AB6538.seq  Check: 989  from: 1  to: 5878



pFN21AB6538  5878 bp

 With 182 enzymes: SgfI * 

                        October 25, 2019 12:32  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3688     278 
   Size:         53      83     186     936    2152    2468

  Fragments arranged by size: 

                2468    2152     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3221    1937 
   Size:       1284    4594
 
Acc65I G'GTAC_C

Cuts at:   3199    3199 
   Size:       5878
 
AclI AA'CG_TT

Cuts at:   1563    4005    4378    1563 
   Size:       2442     373    3063

  Fragments arranged by size: 

                3063    2442     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2517    3941    5325    5593    1632 
   Size:        885    1424    1384     268    1917

  Fragments arranged by size: 

                1917    1424    1384     885     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5681
 
AflIII A'CryG_T

Cuts at:   1203    4777    5754    1203 
   Size:       3574     977    1327

  Fragments arranged by size: 

                3574    1327     977
 
AgeI A'CCGG_T

Cuts at:   4709    4709 
   Size:       5878
 
AhdI GACnn_n'nnGTC

Cuts at:   2392    4607    2392 
   Size:       2215    3663
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5878
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5878
 
AlwNI CAG_nnn'CTG

Cuts at:   2212    2623    2827    3062    3621    5193    2212 
   Size:        411     204     235     559    1572    2897

  Fragments arranged by size: 

                2897    1572     559     411     235     204
 
ApaI G_GGCC'C

Cuts at:   3140    3140 
   Size:       5878
 
ApaLI G'TGCA_C

Cuts at:   3935    5091    3935 
   Size:       1156    4722
 
ApoI r'AATT_y

Cuts at:   1493    2666    3187    3413    3565    5674    1493 
   Size:       1173     521     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1173     521     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4432     160 
   Size:        872    3400    1606

  Fragments arranged by size: 

                3400    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5878
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3203    1870 
   Size:         73    1260    4545

  Fragments arranged by size: 

                4545    1260      73
 
BamHI G'GATC_C

Cuts at:   3208    3575    3208 
   Size:        367    5511
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3199    4654     618 
   Size:        325     217     627    1412    1455    1842

  Fragments arranged by size: 

                1842    1455    1412     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2085    2112    2679    2834    2960    3014    3051    3140 
   Size:        694      27     567     155     126      54      37      89

Cuts at:   3140    3197    1391 
   Size:         57    4072

  Fragments arranged by size: 

                4072     694     567     155     126      89      57      54
                  37      27
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2245    2285    2369    2401    3165     928 
   Size:        971     346      40      84      32     764    3641

  Fragments arranged by size: 

                3641     971     764     346      84      40      32
 
BbvCI CC'TCA_GC

Cuts at:   2859    2859 
   Size:       5878
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4069    4103    1903 
   Size:         34    2132      34    3678

  Fragments arranged by size: 

                3678    2132      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3770    4980    3770 
   Size:       1210    4668
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5878
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4489     136 
   Size:        107     122      71     631     605    2817    1525

  Fragments arranged by size: 

                2817    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5873    5873 
   Size:       5878
 
BlpI GC'TnA_GC

Cuts at:   3045    3295    3045 
   Size:        250    5628
 
Bme1580I G_kGCm'C

Cuts at:   1790    2429    3140    3939    5095    1790 
   Size:        639     711     799    1156    2573

  Fragments arranged by size: 

                2573    1156     799     711     639
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5878
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3045    3609    4567 
   Size:        416      53      83     399      24    1628     564     958

Cuts at:   4567     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1628     958     564     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5878
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2294    2715    2849    4538    1239 
   Size:       1055     421     134    1689    2579

  Fragments arranged by size: 

                2579    1689    1055     421     134
 
Bpu10I CC'TnA_GC

Cuts at:   2681    2859    2681 
   Size:        178    5700
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2645    4003    4868    5166    2645 
   Size:       1358     865     298    3357

  Fragments arranged by size: 

                3357    1358     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4541     882 
   Size:        617    3042    2219

  Fragments arranged by size: 

                3042    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3669     493 
   Size:        711    2465    2702

  Fragments arranged by size: 

                2702    2465     711
 
BsaBI GATnn'nnATC

Cuts at:   5872    5872 
   Size:       5878
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3685    4067    4702     275 
   Size:         53      83     186     936    2152     382     635    1451

  Fragments arranged by size: 

                2152    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2118    4311    4709    4983    5130    5541    2118 
   Size:       2193     398     274     147     411    2455

  Fragments arranged by size: 

                2455    2193     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2096    2126    2801    2816    2831 
   Size:         30     165      30     693      30     675      15      15

Cuts at:   2831    2846    1178 
   Size:         15    4210

  Fragments arranged by size: 

                4210     693     675     165      30      30      30      15
                  15      15
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2122    2567    2570    2621    3044    1186 
   Size:        936     445       3      51     423    4020

  Fragments arranged by size: 

                4020     936     445     423      51       3
 
BseYI C'CCAG_C

Cuts at:   2416    2446    2506    2997    3140    5081    5577    2416 
   Size:         30      60     491     143    1941     496    2717

  Fragments arranged by size: 

                2717    1941     496     491     143      60      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2472    1163 
   Size:        573     736    4569

  Fragments arranged by size: 

                4569     736     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3328    4089    5117    1540 
   Size:        275     186    1327     761    1028    2301

  Fragments arranged by size: 

                2301    1327    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2043    2085    3939    4024    5095    2043 
   Size:         42    1854      85    1071    2826

  Fragments arranged by size: 

                2826    1854    1071      85      42
 
BsmI GAATG_Cn'

Cuts at:   2715    3389    3482    2715 
   Size:        674      93    5111

  Fragments arranged by size: 

                5111     674      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2043    2085    2112    2429    2679    2834    2960 
   Size:        399     253      42      27     317     250     155     126

Cuts at:   2960    3014    3051    3140    3197    3939    4024    5095    1391 
   Size:         54      37      89      57     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     317     253     250     155
                 126      89      85      57      54      42      37      27
            
 
BspEI T'CCGG_A

Cuts at:   2118    5541    2118 
   Size:       3423    2455
 
BspHI T'CATG_A

Cuts at:   3767    5497    3767 
   Size:       1730    4148
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2220    3233     844 
   Size:       1376    1013    3489

  Fragments arranged by size: 

                3489    1376    1013
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2139    2170    2259    3090    3765    1394 
   Size:        429     316      31      89     831     675    3507

  Fragments arranged by size: 

                3507     831     675     429     316      89      31
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2341    2956    4373    4547      65 
   Size:       1183    1093     615    1417     174    1396

  Fragments arranged by size: 

                1417    1396    1183    1093     615     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4522    4709    1700 
   Size:        110    2712     187    2869

  Fragments arranged by size: 

                2869    2712     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5878
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5878
 
BssSI C'ACGA_G

Cuts at:   2422    2549    3938    4950    2422 
   Size:        127    1389    1012    3350

  Fragments arranged by size: 

                3350    1389    1012     127
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5878
 
BstBI TT'CG_AA

Cuts at:   4682    4682 
   Size:       5878
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5878
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2581    2581 
   Size:       5878
 
BstYI r'GATC_y

Cuts at:   1972    2226    3208    3575    3960    3977    5429    5515    5527 
   Size:        254     982     367     385      17    1452      86      12

Cuts at:   5527    5873    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     982     385     367     346     254      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2518    2580    2931     513 
   Size:        552    1453      62     351    3460

  Fragments arranged by size: 

                3460    1453     552     351      62
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2282     487 
   Size:        683      56    1056    4083

  Fragments arranged by size: 

                4083    1056     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2568    3394    4188    4208    2568 
   Size:        826     794      20    4238

  Fragments arranged by size: 

                4238     826     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5878
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5843
 
DraI TTT'AAA

Cuts at:   3182    3182 
   Size:       5878
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4885     809 
   Size:        507    3569    1802

  Fragments arranged by size: 

                3569    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2311    2515    2521 
   Size:         54    1425      50     275      18     481     204       6

Cuts at:   2521    2643    2776    2790    3325    4214    5843       8 
   Size:        122     133      14     535     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     535     481     275     204     133
                 122      54      50      43      18      14       6
 
EagI C'GGCC_G

Cuts at:   1537    1812    3325    1537 
   Size:        275    1513    4090

  Fragments arranged by size: 

                4090    1513     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2177    3808    1353 
   Size:        824    1631    3423

  Fragments arranged by size: 

                3423    1631     824
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3170    4465    4839    4985     387 
   Size:       1064     138    1581    1295     374     146    1280

  Fragments arranged by size: 

                1581    1295    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2083    2083 
   Size:       5878
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2294    2517    2715    2849    3941    4538    5325 
   Size:        393     662     223     198     134    1092     597     787

Cuts at:   5325    5593    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524    1092     787     662     597     393     268     223
                 198     134
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2603    2603 
   Size:       5878
 
EcoO109I rG'GnC_Cy

Cuts at:   2209    2824    3136    3137    2209 
   Size:        615     312       1    4950

  Fragments arranged by size: 

                4950     615     312       1
 
EcoRI G'AATT_C

Cuts at:   2666    3187    3565    2666 
   Size:        521     378    4979

  Fragments arranged by size: 

                4979     521     378
 
FspI TGC'GCA

Cuts at:   2540    4384    2540 
   Size:       1844    4034
 
HaeII r_GCGC'y

Cuts at:   1678    5025    1678 
   Size:       3347    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2096    2128    2378    2405    2410 
   Size:         32     135      32     524      32     250      27       5

Cuts at:   2410    2437    2795    2801    2816    2827    2833    2848    2900 
   Size:         27     358       6      15      11       6      15      52

Cuts at:   2900    2932    3001    3033    4519    4551    4593    4625    1373 
   Size:         32      69      32    1486      32      42      32    2626

  Fragments arranged by size: 

                2626    1486     524     358     250     135      69      52
                  42      32      32      32      32      32      32      32
                  27      27      15      15      11       6       6       5
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3222    3468     669 
   Size:        988     281    1284     246    3079

  Fragments arranged by size: 

                3079    1284     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5878
 
HpaI GTT'AAC

Cuts at:   3468    3468 
   Size:       5878
 
KpnI G_GTAC'C

Cuts at:   3203    3203 
   Size:       5878
 
MfeI C'AATT_G

Cuts at:   3477    3477 
   Size:       5878
 
MluI A'CGCG_T

Cuts at:   5754    5754 
   Size:       5878
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4992    5176    1305 
   Size:       3687     184    2007

  Fragments arranged by size: 

                3687    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2645    2792      10 
   Size:         54    1425     343     813     147    3096

  Fragments arranged by size: 

                3096    1425     813     343     147      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2724    3836    4195    4354     518 
   Size:        648    1558    1112     359     159    2042

  Fragments arranged by size: 

                2042    1558    1112     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2338    2623    3294    3971    5119    5364    1606 
   Size:        732     285     671     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     732     677     671     285     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2580    2931     513 
   Size:        552    1515     351    3460

  Fragments arranged by size: 

                3460    1515     552     351
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5878
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5878
 
NotI GC'GGCC_GC

Cuts at:   3325    3325 
   Size:       5878
 
NspI r_CATG'y

Cuts at:   1132    1229    2437    3236    4781    1132 
   Size:         97    1208     799    1545    2229

  Fragments arranged by size: 

                2229    1545    1208     799      97
 
PasI CC'CwG_GG

Cuts at:   1348    3133    1348 
   Size:       1785    4093
 
PciI A'CATG_T

Cuts at:   4777    4777 
   Size:       5878
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2827    1108 
   Size:       1719    4159
 
PfoI T'CCnGG_A

Cuts at:   2382    2382 
   Size:       5878
 
PmeI GTTT'AAAC

Cuts at:   3182    3182 
   Size:       5878
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3947    3979    5460    5492    3947 
   Size:         32    1481      32    4333

  Fragments arranged by size: 

                4333    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2209    2824    2209 
   Size:        615    5263
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5878
 
PsiI TTA'TAA

Cuts at:   3448    3448 
   Size:       5878
 
PspOMI G'GGCC_C

Cuts at:   3136    3136 
   Size:       5878
 
PstI C_TGCA'G

Cuts at:    830    2403    2628    3230     830 
   Size:       1573     225     602    3478

  Fragments arranged by size: 

                3478    1573     602     225
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5878
 
PvuII CAG'CTG

Cuts at:   2338    2623    2338 
   Size:        285    5593
 
SacI G_AGCT'C

Cuts at:   2085    2085 
   Size:       5878
 
SalI G'TCGA_C

Cuts at:   1936    3220    1936 
   Size:       1284    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2177    2177 
   Size:       5878
 
SbfI CC_TGCA'GG

Cuts at:   2403    3230    2403 
   Size:        827    5051
 
ScaI AGT'ACT

Cuts at:   4126    5838    4126 
   Size:       1712    4166
 
SfcI C'TryA_G

Cuts at:    826    1045    2399    2624    2943    3226    4361    5042    5233 
   Size:        219    1354     225     319     283    1135     681     191

Cuts at:   5233     826 
   Size:       1471

  Fragments arranged by size: 

                1471    1354    1135     681     319     283     225     219
                 191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5878
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5878
 
SmaI CCC'GGG

Cuts at:   1872    3205    1872 
   Size:       1333    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2660    3982    4883    5145     820 
   Size:        197     926     717    1322     901     262    1553

  Fragments arranged by size: 

                1553    1322     926     901     717     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3669     493 
   Size:       3176    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5878
 
SphI G_CATG'C

Cuts at:   1132    3236    1132 
   Size:       2104    3774
 
SspI AAT'ATT

Cuts at:      5      52    3802       5 
   Size:         47    3750    2081

  Fragments arranged by size: 

                3750    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5878
 
StyI C'CwwG_G

Cuts at:    513    1065    2097    2580    2599    2931    3317     513 
   Size:        552    1032     483      19     332     386    3074

  Fragments arranged by size: 

                3074    1032     552     483     386     332      19
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3091    3905    3922    4075    4260    4705     325 
   Size:       1255    1511     814      17     153     185     445    1498

  Fragments arranged by size: 

                1511    1498    1255     814     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3053    4124    5836 
   Size:        274      80      33      51    1443    1076    1071    1712

Cuts at:   5836      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1076    1071     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2048    2080    2360    2392    5838    5870    2048 
   Size:         32     280      32    3446      32    2056

  Fragments arranged by size: 

                3446    2056     280      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5878
 
XbaI T'CTAG_A

Cuts at:   3214    3214 
   Size:       5878
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2011    3148    2011 
   Size:       1137    4741
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5878
 
XmaI C'CCGG_G

Cuts at:   1870    3203    1870 
   Size:       1333    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2240    4007    2240 
   Size:       1767    4111
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3686     276 
   Size:         53      83     186     936    2152    2468

  Fragments arranged by size: 

                2468    2152     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI
     NspI     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI
    PshAI     PsiI   PspOMI     PstI     PvuI    PvuII     SacI     SalI
     SapI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     AscI    AvrII     BaeI     BbeI    BfrBI
     BplI    BsiWI    BsmBI  BstZ17I   Bsu36I   DraIII    EcoRV     FalI
     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     NsiI
     PacI     PmlI    PspXI     PsrI    RsrII    SacII    SanDI    SexAI
     SfiI     SfoI     SrfI     SwaI
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