(Circular) (Six-base) MAPSORT of: pFN21AB6538.seq Check: 989 from: 1 to: 5878 pFN21AB6538 5878 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3688 278 Size: 53 83 186 936 2152 2468 Fragments arranged by size: 2468 2152 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3221 1937 Size: 1284 4594 Acc65I G'GTAC_C Cuts at: 3199 3199 Size: 5878 AclI AA'CG_TT Cuts at: 1563 4005 4378 1563 Size: 2442 373 3063 Fragments arranged by size: 3063 2442 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2517 3941 5325 5593 1632 Size: 885 1424 1384 268 1917 Fragments arranged by size: 1917 1424 1384 885 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5681 AflIII A'CryG_T Cuts at: 1203 4777 5754 1203 Size: 3574 977 1327 Fragments arranged by size: 3574 1327 977 AgeI A'CCGG_T Cuts at: 4709 4709 Size: 5878 AhdI GACnn_n'nnGTC Cuts at: 2392 4607 2392 Size: 2215 3663 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5878 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5878 AlwNI CAG_nnn'CTG Cuts at: 2212 2623 2827 3062 3621 5193 2212 Size: 411 204 235 559 1572 2897 Fragments arranged by size: 2897 1572 559 411 235 204 ApaI G_GGCC'C Cuts at: 3140 3140 Size: 5878 ApaLI G'TGCA_C Cuts at: 3935 5091 3935 Size: 1156 4722 ApoI r'AATT_y Cuts at: 1493 2666 3187 3413 3565 5674 1493 Size: 1173 521 226 152 2109 1697 Fragments arranged by size: 2109 1697 1173 521 226 152 AseI AT'TA_AT Cuts at: 160 1032 4432 160 Size: 872 3400 1606 Fragments arranged by size: 3400 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5878 AvaI C'yCGr_G Cuts at: 1870 1943 3203 1870 Size: 73 1260 4545 Fragments arranged by size: 4545 1260 73 BamHI G'GATC_C Cuts at: 3208 3575 3208 Size: 367 5511 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3199 4654 618 Size: 325 217 627 1412 1455 1842 Fragments arranged by size: 1842 1455 1412 627 325 217 BanII G_rGCy'C Cuts at: 1391 2085 2112 2679 2834 2960 3014 3051 3140 Size: 694 27 567 155 126 54 37 89 Cuts at: 3140 3197 1391 Size: 57 4072 Fragments arranged by size: 4072 694 567 155 126 89 57 54 37 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2245 2285 2369 2401 3165 928 Size: 971 346 40 84 32 764 3641 Fragments arranged by size: 3641 971 764 346 84 40 32 BbvCI CC'TCA_GC Cuts at: 2859 2859 Size: 5878 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4069 4103 1903 Size: 34 2132 34 3678 Fragments arranged by size: 3678 2132 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3770 4980 3770 Size: 1210 4668 BclI T'GATC_A Cuts at: 1548 1548 Size: 5878 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4489 136 Size: 107 122 71 631 605 2817 1525 Fragments arranged by size: 2817 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5873 5873 Size: 5878 BlpI GC'TnA_GC Cuts at: 3045 3295 3045 Size: 250 5628 Bme1580I G_kGCm'C Cuts at: 1790 2429 3140 3939 5095 1790 Size: 639 711 799 1156 2573 Fragments arranged by size: 2573 1156 799 711 639 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5878 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3045 3609 4567 Size: 416 53 83 399 24 1628 564 958 Cuts at: 4567 442 Size: 1753 Fragments arranged by size: 1753 1628 958 564 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5878 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2294 2715 2849 4538 1239 Size: 1055 421 134 1689 2579 Fragments arranged by size: 2579 1689 1055 421 134 Bpu10I CC'TnA_GC Cuts at: 2681 2859 2681 Size: 178 5700 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2645 4003 4868 5166 2645 Size: 1358 865 298 3357 Fragments arranged by size: 3357 1358 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4541 882 Size: 617 3042 2219 Fragments arranged by size: 3042 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3669 493 Size: 711 2465 2702 Fragments arranged by size: 2702 2465 711 BsaBI GATnn'nnATC Cuts at: 5872 5872 Size: 5878 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3685 4067 4702 275 Size: 53 83 186 936 2152 382 635 1451 Fragments arranged by size: 2152 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2118 4311 4709 4983 5130 5541 2118 Size: 2193 398 274 147 411 2455 Fragments arranged by size: 2455 2193 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2096 2126 2801 2816 2831 Size: 30 165 30 693 30 675 15 15 Cuts at: 2831 2846 1178 Size: 15 4210 Fragments arranged by size: 4210 693 675 165 30 30 30 15 15 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2122 2567 2570 2621 3044 1186 Size: 936 445 3 51 423 4020 Fragments arranged by size: 4020 936 445 423 51 3 BseYI C'CCAG_C Cuts at: 2416 2446 2506 2997 3140 5081 5577 2416 Size: 30 60 491 143 1941 496 2717 Fragments arranged by size: 2717 1941 496 491 143 60 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2472 1163 Size: 573 736 4569 Fragments arranged by size: 4569 736 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3328 4089 5117 1540 Size: 275 186 1327 761 1028 2301 Fragments arranged by size: 2301 1327 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2043 2085 3939 4024 5095 2043 Size: 42 1854 85 1071 2826 Fragments arranged by size: 2826 1854 1071 85 42 BsmI GAATG_Cn' Cuts at: 2715 3389 3482 2715 Size: 674 93 5111 Fragments arranged by size: 5111 674 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 2085 2112 2429 2679 2834 2960 Size: 399 253 42 27 317 250 155 126 Cuts at: 2960 3014 3051 3140 3197 3939 4024 5095 1391 Size: 54 37 89 57 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 317 253 250 155 126 89 85 57 54 42 37 27 BspEI T'CCGG_A Cuts at: 2118 5541 2118 Size: 3423 2455 BspHI T'CATG_A Cuts at: 3767 5497 3767 Size: 1730 4148 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2220 3233 844 Size: 1376 1013 3489 Fragments arranged by size: 3489 1376 1013 BsrBI CCG'CTC Cuts at: 1394 1823 2139 2170 2259 3090 3765 1394 Size: 429 316 31 89 831 675 3507 Fragments arranged by size: 3507 831 675 429 316 89 31 BsrDI GCAATG_nn' Cuts at: 65 1248 2341 2956 4373 4547 65 Size: 1183 1093 615 1417 174 1396 Fragments arranged by size: 1417 1396 1183 1093 615 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4522 4709 1700 Size: 110 2712 187 2869 Fragments arranged by size: 2869 2712 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5878 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5878 BssSI C'ACGA_G Cuts at: 2422 2549 3938 4950 2422 Size: 127 1389 1012 3350 Fragments arranged by size: 3350 1389 1012 127 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5878 BstBI TT'CG_AA Cuts at: 4682 4682 Size: 5878 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5878 BstXI CCAn_nnnn'nTGG Cuts at: 2581 2581 Size: 5878 BstYI r'GATC_y Cuts at: 1972 2226 3208 3575 3960 3977 5429 5515 5527 Size: 254 982 367 385 17 1452 86 12 Cuts at: 5527 5873 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 982 385 367 346 254 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2518 2580 2931 513 Size: 552 1453 62 351 3460 Fragments arranged by size: 3460 1453 552 351 62 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2282 487 Size: 683 56 1056 4083 Fragments arranged by size: 4083 1056 683 56 BtsI GCAGTG_nn' Cuts at: 2568 3394 4188 4208 2568 Size: 826 794 20 4238 Fragments arranged by size: 4238 826 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5878 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5843 DraI TTT'AAA Cuts at: 3182 3182 Size: 5878 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4885 809 Size: 507 3569 1802 Fragments arranged by size: 3569 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2311 2515 2521 Size: 54 1425 50 275 18 481 204 6 Cuts at: 2521 2643 2776 2790 3325 4214 5843 8 Size: 122 133 14 535 889 1629 43 Fragments arranged by size: 1629 1425 889 535 481 275 204 133 122 54 50 43 18 14 6 EagI C'GGCC_G Cuts at: 1537 1812 3325 1537 Size: 275 1513 4090 Fragments arranged by size: 4090 1513 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2177 3808 1353 Size: 824 1631 3423 Fragments arranged by size: 3423 1631 824 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3170 4465 4839 4985 387 Size: 1064 138 1581 1295 374 146 1280 Fragments arranged by size: 1581 1295 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2083 2083 Size: 5878 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2294 2517 2715 2849 3941 4538 5325 Size: 393 662 223 198 134 1092 597 787 Cuts at: 5325 5593 1239 Size: 268 1524 Fragments arranged by size: 1524 1092 787 662 597 393 268 223 198 134 EcoNI CCTnn'n_nnAGG Cuts at: 2603 2603 Size: 5878 EcoO109I rG'GnC_Cy Cuts at: 2209 2824 3136 3137 2209 Size: 615 312 1 4950 Fragments arranged by size: 4950 615 312 1 EcoRI G'AATT_C Cuts at: 2666 3187 3565 2666 Size: 521 378 4979 Fragments arranged by size: 4979 521 378 FspI TGC'GCA Cuts at: 2540 4384 2540 Size: 1844 4034 HaeII r_GCGC'y Cuts at: 1678 5025 1678 Size: 3347 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2096 2128 2378 2405 2410 Size: 32 135 32 524 32 250 27 5 Cuts at: 2410 2437 2795 2801 2816 2827 2833 2848 2900 Size: 27 358 6 15 11 6 15 52 Cuts at: 2900 2932 3001 3033 4519 4551 4593 4625 1373 Size: 32 69 32 1486 32 42 32 2626 Fragments arranged by size: 2626 1486 524 358 250 135 69 52 42 32 32 32 32 32 32 32 27 27 15 15 11 6 6 5 HincII GTy'rAC Cuts at: 669 1657 1938 3222 3468 669 Size: 988 281 1284 246 3079 Fragments arranged by size: 3079 1284 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5878 HpaI GTT'AAC Cuts at: 3468 3468 Size: 5878 KpnI G_GTAC'C Cuts at: 3203 3203 Size: 5878 MfeI C'AATT_G Cuts at: 3477 3477 Size: 5878 MluI A'CGCG_T Cuts at: 5754 5754 Size: 5878 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4992 5176 1305 Size: 3687 184 2007 Fragments arranged by size: 3687 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2645 2792 10 Size: 54 1425 343 813 147 3096 Fragments arranged by size: 3096 1425 813 343 147 54 MslI CAynn'nnrTG Cuts at: 518 1166 2724 3836 4195 4354 518 Size: 648 1558 1112 359 159 2042 Fragments arranged by size: 2042 1558 1112 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2338 2623 3294 3971 5119 5364 1606 Size: 732 285 671 677 1148 245 2120 Fragments arranged by size: 2120 1148 732 677 671 285 245 NcoI C'CATG_G Cuts at: 513 1065 2580 2931 513 Size: 552 1515 351 3460 Fragments arranged by size: 3460 1515 552 351 NdeI CA'TA_TG Cuts at: 387 387 Size: 5878 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5878 NotI GC'GGCC_GC Cuts at: 3325 3325 Size: 5878 NspI r_CATG'y Cuts at: 1132 1229 2437 3236 4781 1132 Size: 97 1208 799 1545 2229 Fragments arranged by size: 2229 1545 1208 799 97 PasI CC'CwG_GG Cuts at: 1348 3133 1348 Size: 1785 4093 PciI A'CATG_T Cuts at: 4777 4777 Size: 5878 PflMI CCAn_nnn'nTGG Cuts at: 1108 2827 1108 Size: 1719 4159 PfoI T'CCnGG_A Cuts at: 2382 2382 Size: 5878 PmeI GTTT'AAAC Cuts at: 3182 3182 Size: 5878 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3947 3979 5460 5492 3947 Size: 32 1481 32 4333 Fragments arranged by size: 4333 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2209 2824 2209 Size: 615 5263 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5878 PsiI TTA'TAA Cuts at: 3448 3448 Size: 5878 PspOMI G'GGCC_C Cuts at: 3136 3136 Size: 5878 PstI C_TGCA'G Cuts at: 830 2403 2628 3230 830 Size: 1573 225 602 3478 Fragments arranged by size: 3478 1573 602 225 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5878 PvuII CAG'CTG Cuts at: 2338 2623 2338 Size: 285 5593 SacI G_AGCT'C Cuts at: 2085 2085 Size: 5878 SalI G'TCGA_C Cuts at: 1936 3220 1936 Size: 1284 4594 SapI GCTCTTCn'nnn_ Cuts at: 2177 2177 Size: 5878 SbfI CC_TGCA'GG Cuts at: 2403 3230 2403 Size: 827 5051 ScaI AGT'ACT Cuts at: 4126 5838 4126 Size: 1712 4166 SfcI C'TryA_G Cuts at: 826 1045 2399 2624 2943 3226 4361 5042 5233 Size: 219 1354 225 319 283 1135 681 191 Cuts at: 5233 826 Size: 1471 Fragments arranged by size: 1471 1354 1135 681 319 283 225 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5878 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5878 SmaI CCC'GGG Cuts at: 1872 3205 1872 Size: 1333 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2660 3982 4883 5145 820 Size: 197 926 717 1322 901 262 1553 Fragments arranged by size: 1553 1322 926 901 717 262 197 SnaBI TAC'GTA Cuts at: 493 3669 493 Size: 3176 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5878 SphI G_CATG'C Cuts at: 1132 3236 1132 Size: 2104 3774 SspI AAT'ATT Cuts at: 5 52 3802 5 Size: 47 3750 2081 Fragments arranged by size: 3750 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5878 StyI C'CwwG_G Cuts at: 513 1065 2097 2580 2599 2931 3317 513 Size: 552 1032 483 19 332 386 3074 Fragments arranged by size: 3074 1032 552 483 386 332 19 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3091 3905 3922 4075 4260 4705 325 Size: 1255 1511 814 17 153 185 445 1498 Fragments arranged by size: 1511 1498 1255 814 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3053 4124 5836 Size: 274 80 33 51 1443 1076 1071 1712 Cuts at: 5836 96 Size: 138 Fragments arranged by size: 1712 1443 1076 1071 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2048 2080 2360 2392 5838 5870 2048 Size: 32 280 32 3446 32 2056 Fragments arranged by size: 3446 2056 280 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5878 XbaI T'CTAG_A Cuts at: 3214 3214 Size: 5878 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 3148 2011 Size: 1137 4741 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5878 XmaI C'CCGG_G Cuts at: 1870 3203 1870 Size: 1333 4545 XmnI GAAnn'nnTTC Cuts at: 2240 4007 2240 Size: 1767 4111 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3686 276 Size: 53 83 186 936 2152 2468 Fragments arranged by size: 2468 2152 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI