Restriction map for
pFN21AE4354
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AlwNI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BbeI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaHI BsaI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MfeI MluI
MroI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcsI 691 AAATTT [GA]^AATT[CT]
AflIII 409 ACGTGT A^C[GA][CT]GT
Alw44I 210 GTGCAC G^TGCAC
AocI 922 CCTGAGG CC^T[ACGT]AGG
ApaLI 210 GTGCAC G^TGCAC
ApoI 691 AAATTT [GA]^AATT[CT]
AspHI 210 GTGCAC G[AT]GC[AT]^C
AvaI 817 CCCGGG C^[CT]CG[GA]G
BamHI 1015 GGATCC G^GATCC
BanI 248 GGTGCC G^G[CT][GA]CC
BbrPI 370 CACGTG CAC^GTG
BfaI 256 CTAG C^TAG
BsaBI 93 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 455 ACCGGA [AT]^CCGG[AT]
BsiHKAI 210 GTGCAC G[AT]GC[AT]^C
BsmBI 272 GAGACG CGTCTC(1/5)
BsmFI 304 GGGAC GGGAC(10/14)
BsmI 138 GAATGC GAATGC(1/-1)
BsoBI 817 CCCGGG C^[CT]CG[GA]G
BsrBI 1095 CCGCTC CCGCTC(-3/-3)
Bsu36I 922 CCTGAGG CC^T[ACGT]AGG
DdeI 923 CTGAG C^T[ACGT]AG
DraIII 471 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 922 CCTGAGG CC^T[ACGT]AGG
MaeI 256 CTAG C^TAG
MamI 93 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 700 TTAA T^TAA
MwoI 9 GCTGGTTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 619 ACATGC [GA]CATG^[CT]
PleI 964 GAGTC GAGTC(4/5)
PmaCI 370 CACGTG CAC^GTG
PmlI 370 CACGTG CAC^GTG
SfcI 177 CTACAG C^T[GA][CT]AG
SmaI 817 CCCGGG CCC^GGG
TaqI 467 TCGA T^CGA
Tru9I 700 TTAA T^TAA
TthHB8I 467 TCGA T^CGA
XmaCI 817 CCCGGG C^CCGGG
XmaI 817 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AfaI 88 GTAC GT^AC
453 GTAC
BalI 713 TGGCCA TGG^CCA
947 TGGCCA
BcnI 817 CCCGG CC^[GC]GG
818 CCGGG
BsaAI 370 CACGTG [CT]AC^GT[GA]
1102 TACGTG
BstXI 598 CCAAGCGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
971 CCAGGATGCTGG
BstYI 432 GGATCT [GA]^GATC[CT]
1015 GGATCC
EaeI 713 TGGCCA [CT]^GGCC[GA]
947 TGGCCA
EcoNI 222 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
438 CCTCTATGAGG
EcoT14I 837 CCAAGG C^C[AT][AT]GG
1044 CCAAGG
HapII 456 CCGG C^CGG
818 CCGG
HgaI 259 GACGC GACGC(5/10)
306 GACGC
HpaII 456 CCGG C^CGG
818 CCGG
MboII 771 GAAGA GAAGA(8/7)
812 TCTTC
MflI 432 GGATCT [GA]^GATC[CT]
1015 GGATCC
MluNI 713 TGGCCA TGG^CCA
947 TGGCCA
MscI 713 TGGCCA TGG^CCA
947 TGGCCA
MslI 388 CATCAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1077 CACCTTTGTG
MspI 456 CCGG C^CGG
818 CCGG
NciI 817 CCCGG CC^[GC]GG
818 CCGGG
NlaIII 134 CATG CATG^
620 CATG
PflMI 870 CCAGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
888 CCACAAGATGG
RsaI 88 GTAC GT^AC
453 GTAC
StuI 197 AGGCCT AGG^CCT
1004 AGGCCT
StyI 837 CCAAGG C^C[AT][AT]GG
1044 CCAAGG
TspRI 208 CAGTG CA[GC]TG[ACGT][ACGT]^
398 CAGTG
Van91I 870 CCAGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
888 CCACAAGATGG
XcmI 512 CCATAGAAGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
716 CCATCCTTCTGATGG
XhoII 432 GGATCT [GA]^GATC[CT]
1015 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BbvI BmyI BpmI BsaJI BseRI
BsgI BsiYI BslI BsmAI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DpnII EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeII MaeIII MboI MnlI MvaI
NdeII NlaIV Sau3AI Sau96I ScrFI
SfaNI TfiI TseI Tsp45I Tsp509I