(Circular) (Six-base) MAPSORT of: pFN21AE4354.seq Check: 8926 from: 1 to: 5818 pFN21AE4354 5818 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2122 2894 2122 Size: 772 5046 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3628 278 Size: 53 83 186 936 2092 2468 Fragments arranged by size: 2468 2092 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3161 1937 Size: 1224 4594 Acc65I G'GTAC_C Cuts at: 3139 3139 Size: 5818 AclI AA'CG_TT Cuts at: 1563 3945 4318 1563 Size: 2382 373 3063 Fragments arranged by size: 3063 2382 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2982 3881 5265 5533 1632 Size: 1350 899 1384 268 1917 Fragments arranged by size: 1917 1384 1350 899 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5621 AflIII A'CryG_T Cuts at: 1203 2414 4717 5694 1203 Size: 1211 2303 977 1327 Fragments arranged by size: 2303 1327 1211 977 AgeI A'CCGG_T Cuts at: 4649 4649 Size: 5818 AhdI GACnn_n'nnGTC Cuts at: 4547 4547 Size: 5818 AleI CACnn'nnGTG Cuts at: 1166 3086 1166 Size: 1920 3898 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2415 1184 Size: 1231 4587 AlwNI CAG_nnn'CTG Cuts at: 3561 5133 3561 Size: 1572 4246 ApaLI G'TGCA_C Cuts at: 2215 3875 5031 2215 Size: 1660 1156 3002 Fragments arranged by size: 3002 1660 1156 ApoI r'AATT_y Cuts at: 1493 2696 3127 3353 3505 5614 1493 Size: 1203 431 226 152 2109 1697 Fragments arranged by size: 2109 1697 1203 431 226 152 AseI AT'TA_AT Cuts at: 160 1032 4372 160 Size: 872 3340 1606 Fragments arranged by size: 3340 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5818 AvaI C'yCGr_G Cuts at: 1870 1943 2822 3143 1870 Size: 73 879 321 4545 Fragments arranged by size: 4545 879 321 73 BamHI G'GATC_C Cuts at: 3020 3148 3515 3020 Size: 128 367 5323 Fragments arranged by size: 5323 367 128 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2253 3139 4594 618 Size: 325 217 627 466 886 1455 1842 Fragments arranged by size: 1842 1455 886 627 466 325 217 BanII G_rGCy'C Cuts at: 1391 2468 2829 2937 3137 1391 Size: 1077 361 108 200 4072 Fragments arranged by size: 4072 1077 361 200 108 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4847 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4009 4043 1903 Size: 34 2072 34 3678 Fragments arranged by size: 3678 2072 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3710 4920 3710 Size: 1210 4608 BclI T'GATC_A Cuts at: 1548 1548 Size: 5818 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4429 136 Size: 107 122 71 631 605 2757 1525 Fragments arranged by size: 2757 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5813 5813 Size: 5818 BlpI GC'TnA_GC Cuts at: 3235 3235 Size: 5818 Bme1580I G_kGCm'C Cuts at: 1790 2219 3879 5035 1790 Size: 429 1660 1156 2573 Fragments arranged by size: 2573 1660 1156 429 BmgBI CAC'GTC Cuts at: 1318 2415 1318 Size: 1097 4721 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2355 3549 4507 Size: 416 53 83 399 24 938 1194 958 Cuts at: 4507 442 Size: 1753 Fragments arranged by size: 1753 1194 958 938 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5818 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2143 2216 3011 4478 1239 Size: 904 73 795 1467 2579 Fragments arranged by size: 2579 1467 904 795 73 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3943 4808 5106 3943 Size: 865 298 4655 Fragments arranged by size: 4655 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4481 882 Size: 617 2982 2219 Fragments arranged by size: 2982 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2377 3109 3609 493 Size: 711 1173 732 500 2702 Fragments arranged by size: 2702 1173 732 711 500 BsaBI GATnn'nnATC Cuts at: 2102 5812 2102 Size: 3710 2108 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3625 4007 4642 275 Size: 53 83 186 936 2092 382 635 1451 Fragments arranged by size: 2092 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2460 4251 4649 4923 5070 5481 2460 Size: 1791 398 274 147 411 2797 Fragments arranged by size: 2797 1791 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2022 2052 1178 Size: 30 165 30 619 30 4944 Fragments arranged by size: 4944 619 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2091 2432 2732 2945 3014 1186 Size: 905 341 300 213 69 3990 Fragments arranged by size: 3990 905 341 300 213 69 BseYI C'CCAG_C Cuts at: 2047 2792 5021 5517 2047 Size: 745 2229 496 2348 Fragments arranged by size: 2348 2229 745 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2150 2679 2928 1163 Size: 573 414 529 249 4053 Fragments arranged by size: 4053 573 529 414 249 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3268 4029 5057 1540 Size: 275 186 1267 761 1028 2301 Fragments arranged by size: 2301 1267 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2219 3879 3964 5035 2219 Size: 1660 85 1071 3002 Fragments arranged by size: 3002 1660 1071 85 BsmI GAATG_Cn' Cuts at: 2149 3329 3422 2149 Size: 1180 93 4545 Fragments arranged by size: 4545 1180 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2271 2271 Size: 5818 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2219 2468 2829 2937 3137 3879 3964 Size: 399 429 249 361 108 200 742 85 Cuts at: 3964 5035 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 429 399 361 249 200 108 85 BspEI T'CCGG_A Cuts at: 5481 5481 Size: 5818 BspHI T'CATG_A Cuts at: 3707 5437 3707 Size: 1730 4088 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2122 2513 2894 3173 844 Size: 1278 391 381 279 3489 Fragments arranged by size: 3489 1278 391 381 279 BsrBI CCG'CTC Cuts at: 1394 1823 3102 3705 1394 Size: 429 1279 603 3507 Fragments arranged by size: 3507 1279 603 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4313 4487 65 Size: 1183 3065 174 1396 Fragments arranged by size: 3065 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4462 4649 1700 Size: 110 2652 187 2869 Fragments arranged by size: 2869 2652 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5818 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5818 BssSI C'ACGA_G Cuts at: 3878 4890 3878 Size: 1012 4806 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5818 BstBI TT'CG_AA Cuts at: 4622 4622 Size: 5818 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5818 BstXI CCAn_nnnn'nTGG Cuts at: 2610 2983 2610 Size: 373 5445 BstYI r'GATC_y Cuts at: 1972 2437 3020 3148 3515 3900 3917 5369 5455 Size: 465 583 128 367 385 17 1452 86 Cuts at: 5455 5467 5813 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 583 465 385 367 346 128 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2928 2928 Size: 5818 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4327 Fragments arranged by size: 4327 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5079 Fragments arranged by size: 5079 683 56 BtsI GCAGTG_nn' Cuts at: 2409 3334 4128 4148 2409 Size: 925 794 20 4079 Fragments arranged by size: 4079 925 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5818 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5783 DraI TTT'AAA Cuts at: 3122 3122 Size: 5818 DraIII CAC_nnn'GTG Cuts at: 2481 2481 Size: 5818 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4825 809 Size: 507 3509 1802 Fragments arranged by size: 3509 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2718 2952 3265 Size: 54 1425 50 275 18 888 234 313 Cuts at: 3265 4154 5783 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 888 313 275 234 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3265 1537 Size: 275 1453 4090 Fragments arranged by size: 4090 1453 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3748 1353 Size: 2395 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4405 4779 4925 387 Size: 1064 138 2816 374 146 1280 Fragments arranged by size: 2816 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2143 2216 2982 3011 3881 4478 5265 Size: 393 511 73 766 29 870 597 787 Cuts at: 5265 5533 1239 Size: 268 1524 Fragments arranged by size: 1524 870 787 766 597 511 393 268 73 29 EcoNI CCTnn'n_nnAGG Cuts at: 2231 2447 2231 Size: 216 5602 EcoRI G'AATT_C Cuts at: 3127 3505 3127 Size: 378 5440 FspI TGC'GCA Cuts at: 4324 4324 Size: 5818 HaeII r_GCGC'y Cuts at: 1678 4965 1678 Size: 3287 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2022 2054 2471 2503 2722 Size: 32 135 32 450 32 417 32 219 Cuts at: 2722 2754 2795 2827 4459 4491 4533 4565 1373 Size: 32 41 32 1632 32 42 32 2626 Fragments arranged by size: 2626 1632 450 417 219 135 42 41 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3162 3408 669 Size: 988 281 1224 246 3079 Fragments arranged by size: 3079 1224 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5818 HpaI GTT'AAC Cuts at: 3408 3408 Size: 5818 KpnI G_GTAC'C Cuts at: 3143 3143 Size: 5818 MfeI C'AATT_G Cuts at: 3417 3417 Size: 5818 MluI A'CGCG_T Cuts at: 5694 5694 Size: 5818 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4932 5116 1305 Size: 3627 184 2007 Fragments arranged by size: 3627 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2720 2954 10 Size: 54 1425 343 888 234 2874 Fragments arranged by size: 2874 1425 888 343 234 54 MslI CAynn'nnrTG Cuts at: 518 1166 2397 3086 3776 4135 4294 518 Size: 648 1231 689 690 359 159 2042 Fragments arranged by size: 2042 1231 690 689 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3234 3911 5059 5304 1606 Size: 1628 677 1148 245 2120 Fragments arranged by size: 2120 1628 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4327 Fragments arranged by size: 4327 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5818 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5818 NotI GC'GGCC_GC Cuts at: 3265 3265 Size: 5818 NspI r_CATG'y Cuts at: 1132 1229 2628 3176 4721 1132 Size: 97 1399 548 1545 2229 Fragments arranged by size: 2229 1545 1399 548 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5818 PciI A'CATG_T Cuts at: 4717 4717 Size: 5818 PflMI CCAn_nnn'nTGG Cuts at: 1108 2881 2899 1108 Size: 1773 18 4027 Fragments arranged by size: 4027 1773 18 PfoI T'CCnGG_A Cuts at: 2159 2232 2853 2159 Size: 73 621 5124 Fragments arranged by size: 5124 621 73 PmeI GTTT'AAAC Cuts at: 3122 3122 Size: 5818 PmlI CAC'GTG Cuts at: 2377 2377 Size: 5818 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3887 3919 5400 5432 3887 Size: 32 1481 32 4273 Fragments arranged by size: 4273 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5818 PsiI TTA'TAA Cuts at: 3388 3388 Size: 5818 PstI C_TGCA'G Cuts at: 830 3170 830 Size: 2340 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5818 SalI G'TCGA_C Cuts at: 1936 3160 1936 Size: 1224 4594 SbfI CC_TGCA'GG Cuts at: 3170 3170 Size: 5818 ScaI AGT'ACT Cuts at: 4066 5778 4066 Size: 1712 4106 SfcI C'TryA_G Cuts at: 826 1045 2182 3166 4301 4982 5173 826 Size: 219 1137 984 1135 681 191 1471 Fragments arranged by size: 1471 1137 1135 984 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5818 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5818 SmaI CCC'GGG Cuts at: 1872 2824 3145 1872 Size: 952 321 4545 Fragments arranged by size: 4545 952 321 SmlI C'TyrA_G Cuts at: 820 1017 1943 3922 4823 5085 820 Size: 197 926 1979 901 262 1553 Fragments arranged by size: 1979 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3609 493 Size: 3116 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5818 SphI G_CATG'C Cuts at: 1132 3176 1132 Size: 2044 3774 SspI AAT'ATT Cuts at: 5 52 3742 5 Size: 47 3690 2081 Fragments arranged by size: 3690 2081 47 StuI AGG'CCT Cuts at: 1517 2204 3011 1517 Size: 687 807 4324 Fragments arranged by size: 4324 807 687 StyI C'CwwG_G Cuts at: 513 1065 2004 2842 3049 3257 513 Size: 552 939 838 207 208 3074 Fragments arranged by size: 3074 939 838 552 208 207 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2888 3011 3845 3862 4015 4200 4645 Size: 1255 1308 123 834 17 153 185 445 Cuts at: 4645 325 Size: 1498 Fragments arranged by size: 1498 1308 1255 834 445 185 153 123 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2092 4064 5776 Size: 274 80 33 51 1443 115 1972 1712 Cuts at: 5776 96 Size: 138 Fragments arranged by size: 1972 1712 1443 274 138 115 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5778 5810 5778 Size: 32 5786 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5818 XbaI T'CTAG_A Cuts at: 3154 3154 Size: 5818 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2524 2728 2011 Size: 513 204 5101 Fragments arranged by size: 5101 513 204 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5818 XmaI C'CCGG_G Cuts at: 1870 2822 3143 1870 Size: 952 321 4545 Fragments arranged by size: 4545 952 321 XmnI GAAnn'nnTTC Cuts at: 3947 3947 Size: 5818 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3626 276 Size: 53 83 186 936 2092 2468 Fragments arranged by size: 2468 2092 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI