Restriction map for pFN21AE4354
 (Circular) (Six-base) MAPSORT of: pFN21AE4354.seq  Check: 8926  from: 1  to: 5818



pFN21AE4354  5818 bp

 With 182 enzymes: SgfI * 

                        July 6, 2012 17:39  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2122    2894    2122 
   Size:        772    5046
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3628     278 
   Size:         53      83     186     936    2092    2468

  Fragments arranged by size: 

                2468    2092     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3161    1937 
   Size:       1224    4594
 
Acc65I G'GTAC_C

Cuts at:   3139    3139 
   Size:       5818
 
AclI AA'CG_TT

Cuts at:   1563    3945    4318    1563 
   Size:       2382     373    3063

  Fragments arranged by size: 

                3063    2382     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2982    3881    5265    5533    1632 
   Size:       1350     899    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1350     899     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5621
 
AflIII A'CryG_T

Cuts at:   1203    2414    4717    5694    1203 
   Size:       1211    2303     977    1327

  Fragments arranged by size: 

                2303    1327    1211     977
 
AgeI A'CCGG_T

Cuts at:   4649    4649 
   Size:       5818
 
AhdI GACnn_n'nnGTC

Cuts at:   4547    4547 
   Size:       5818
 
AleI CACnn'nnGTG

Cuts at:   1166    3086    1166 
   Size:       1920    3898
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2415    1184 
   Size:       1231    4587
 
AlwNI CAG_nnn'CTG

Cuts at:   3561    5133    3561 
   Size:       1572    4246
 
ApaLI G'TGCA_C

Cuts at:   2215    3875    5031    2215 
   Size:       1660    1156    3002

  Fragments arranged by size: 

                3002    1660    1156
 
ApoI r'AATT_y

Cuts at:   1493    2696    3127    3353    3505    5614    1493 
   Size:       1203     431     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1203     431     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4372     160 
   Size:        872    3340    1606

  Fragments arranged by size: 

                3340    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5818
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2822    3143    1870 
   Size:         73     879     321    4545

  Fragments arranged by size: 

                4545     879     321      73
 
BamHI G'GATC_C

Cuts at:   3020    3148    3515    3020 
   Size:        128     367    5323

  Fragments arranged by size: 

                5323     367     128
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2253    3139    4594     618 
   Size:        325     217     627     466     886    1455    1842

  Fragments arranged by size: 

                1842    1455     886     627     466     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2468    2829    2937    3137    1391 
   Size:       1077     361     108     200    4072

  Fragments arranged by size: 

                4072    1077     361     200     108
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4847
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4009    4043    1903 
   Size:         34    2072      34    3678

  Fragments arranged by size: 

                3678    2072      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3710    4920    3710 
   Size:       1210    4608
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5818
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4429     136 
   Size:        107     122      71     631     605    2757    1525

  Fragments arranged by size: 

                2757    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5813    5813 
   Size:       5818
 
BlpI GC'TnA_GC

Cuts at:   3235    3235 
   Size:       5818
 
Bme1580I G_kGCm'C

Cuts at:   1790    2219    3879    5035    1790 
   Size:        429    1660    1156    2573

  Fragments arranged by size: 

                2573    1660    1156     429
 
BmgBI CAC'GTC

Cuts at:   1318    2415    1318 
   Size:       1097    4721
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2355    3549    4507 
   Size:        416      53      83     399      24     938    1194     958

Cuts at:   4507     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1194     958     938     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5818
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2143    2216    3011    4478    1239 
   Size:        904      73     795    1467    2579

  Fragments arranged by size: 

                2579    1467     904     795      73
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3943    4808    5106    3943 
   Size:        865     298    4655

  Fragments arranged by size: 

                4655     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4481     882 
   Size:        617    2982    2219

  Fragments arranged by size: 

                2982    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2377    3109    3609     493 
   Size:        711    1173     732     500    2702

  Fragments arranged by size: 

                2702    1173     732     711     500
 
BsaBI GATnn'nnATC

Cuts at:   2102    5812    2102 
   Size:       3710    2108
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3625    4007    4642     275 
   Size:         53      83     186     936    2092     382     635    1451

  Fragments arranged by size: 

                2092    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2460    4251    4649    4923    5070    5481    2460 
   Size:       1791     398     274     147     411    2797

  Fragments arranged by size: 

                2797    1791     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2022    2052    1178 
   Size:         30     165      30     619      30    4944

  Fragments arranged by size: 

                4944     619     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2091    2432    2732    2945    3014    1186 
   Size:        905     341     300     213      69    3990

  Fragments arranged by size: 

                3990     905     341     300     213      69
 
BseYI C'CCAG_C

Cuts at:   2047    2792    5021    5517    2047 
   Size:        745    2229     496    2348

  Fragments arranged by size: 

                2348    2229     745     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2150    2679    2928    1163 
   Size:        573     414     529     249    4053

  Fragments arranged by size: 

                4053     573     529     414     249
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3268    4029    5057    1540 
   Size:        275     186    1267     761    1028    2301

  Fragments arranged by size: 

                2301    1267    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2219    3879    3964    5035    2219 
   Size:       1660      85    1071    3002

  Fragments arranged by size: 

                3002    1660    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2149    3329    3422    2149 
   Size:       1180      93    4545

  Fragments arranged by size: 

                4545    1180      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2271    2271 
   Size:       5818
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2219    2468    2829    2937    3137    3879    3964 
   Size:        399     429     249     361     108     200     742      85

Cuts at:   3964    5035    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     742     429     399     361     249     200
                 108      85
 
BspEI T'CCGG_A

Cuts at:   5481    5481 
   Size:       5818
 
BspHI T'CATG_A

Cuts at:   3707    5437    3707 
   Size:       1730    4088
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2122    2513    2894    3173     844 
   Size:       1278     391     381     279    3489

  Fragments arranged by size: 

                3489    1278     391     381     279
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3102    3705    1394 
   Size:        429    1279     603    3507

  Fragments arranged by size: 

                3507    1279     603     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4313    4487      65 
   Size:       1183    3065     174    1396

  Fragments arranged by size: 

                3065    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4462    4649    1700 
   Size:        110    2652     187    2869

  Fragments arranged by size: 

                2869    2652     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5818
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5818
 
BssSI C'ACGA_G

Cuts at:   3878    4890    3878 
   Size:       1012    4806
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5818
 
BstBI TT'CG_AA

Cuts at:   4622    4622 
   Size:       5818
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5818
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2610    2983    2610 
   Size:        373    5445
 
BstYI r'GATC_y

Cuts at:   1972    2437    3020    3148    3515    3900    3917    5369    5455 
   Size:        465     583     128     367     385      17    1452      86

Cuts at:   5455    5467    5813    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     583     465     385     367     346     128
                  86      17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2928    2928 
   Size:       5818
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4327

  Fragments arranged by size: 

                4327     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5079

  Fragments arranged by size: 

                5079     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2409    3334    4128    4148    2409 
   Size:        925     794      20    4079

  Fragments arranged by size: 

                4079     925     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5818
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5783
 
DraI TTT'AAA

Cuts at:   3122    3122 
   Size:       5818
 
DraIII CAC_nnn'GTG

Cuts at:   2481    2481 
   Size:       5818
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4825     809 
   Size:        507    3509    1802

  Fragments arranged by size: 

                3509    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2718    2952    3265 
   Size:         54    1425      50     275      18     888     234     313

Cuts at:   3265    4154    5783       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     888     313     275     234      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3265    1537 
   Size:        275    1453    4090

  Fragments arranged by size: 

                4090    1453     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3748    1353 
   Size:       2395    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4405    4779    4925     387 
   Size:       1064     138    2816     374     146    1280

  Fragments arranged by size: 

                2816    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2143    2216    2982    3011    3881    4478    5265 
   Size:        393     511      73     766      29     870     597     787

Cuts at:   5265    5533    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524     870     787     766     597     511     393     268
                  73      29
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2231    2447    2231 
   Size:        216    5602
 
EcoRI G'AATT_C

Cuts at:   3127    3505    3127 
   Size:        378    5440
 
FspI TGC'GCA

Cuts at:   4324    4324 
   Size:       5818
 
HaeII r_GCGC'y

Cuts at:   1678    4965    1678 
   Size:       3287    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2022    2054    2471    2503    2722 
   Size:         32     135      32     450      32     417      32     219

Cuts at:   2722    2754    2795    2827    4459    4491    4533    4565    1373 
   Size:         32      41      32    1632      32      42      32    2626

  Fragments arranged by size: 

                2626    1632     450     417     219     135      42      41
                  32      32      32      32      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3162    3408     669 
   Size:        988     281    1224     246    3079

  Fragments arranged by size: 

                3079    1224     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5818
 
HpaI GTT'AAC

Cuts at:   3408    3408 
   Size:       5818
 
KpnI G_GTAC'C

Cuts at:   3143    3143 
   Size:       5818
 
MfeI C'AATT_G

Cuts at:   3417    3417 
   Size:       5818
 
MluI A'CGCG_T

Cuts at:   5694    5694 
   Size:       5818
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4932    5116    1305 
   Size:       3627     184    2007

  Fragments arranged by size: 

                3627    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2720    2954      10 
   Size:         54    1425     343     888     234    2874

  Fragments arranged by size: 

                2874    1425     888     343     234      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2397    3086    3776    4135    4294     518 
   Size:        648    1231     689     690     359     159    2042

  Fragments arranged by size: 

                2042    1231     690     689     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3234    3911    5059    5304    1606 
   Size:       1628     677    1148     245    2120

  Fragments arranged by size: 

                2120    1628    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4327

  Fragments arranged by size: 

                4327     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5818
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5818
 
NotI GC'GGCC_GC

Cuts at:   3265    3265 
   Size:       5818
 
NspI r_CATG'y

Cuts at:   1132    1229    2628    3176    4721    1132 
   Size:         97    1399     548    1545    2229

  Fragments arranged by size: 

                2229    1545    1399     548      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5818
 
PciI A'CATG_T

Cuts at:   4717    4717 
   Size:       5818
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2881    2899    1108 
   Size:       1773      18    4027

  Fragments arranged by size: 

                4027    1773      18
 
PfoI T'CCnGG_A

Cuts at:   2159    2232    2853    2159 
   Size:         73     621    5124

  Fragments arranged by size: 

                5124     621      73
 
PmeI GTTT'AAAC

Cuts at:   3122    3122 
   Size:       5818
 
PmlI CAC'GTG

Cuts at:   2377    2377 
   Size:       5818
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3887    3919    5400    5432    3887 
   Size:         32    1481      32    4273

  Fragments arranged by size: 

                4273    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5818
 
PsiI TTA'TAA

Cuts at:   3388    3388 
   Size:       5818
 
PstI C_TGCA'G

Cuts at:    830    3170     830 
   Size:       2340    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5818
 
SalI G'TCGA_C

Cuts at:   1936    3160    1936 
   Size:       1224    4594
 
SbfI CC_TGCA'GG

Cuts at:   3170    3170 
   Size:       5818
 
ScaI AGT'ACT

Cuts at:   4066    5778    4066 
   Size:       1712    4106
 
SfcI C'TryA_G

Cuts at:    826    1045    2182    3166    4301    4982    5173     826 
   Size:        219    1137     984    1135     681     191    1471

  Fragments arranged by size: 

                1471    1137    1135     984     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5818
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5818
 
SmaI CCC'GGG

Cuts at:   1872    2824    3145    1872 
   Size:        952     321    4545

  Fragments arranged by size: 

                4545     952     321
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3922    4823    5085     820 
   Size:        197     926    1979     901     262    1553

  Fragments arranged by size: 

                1979    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3609     493 
   Size:       3116    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5818
 
SphI G_CATG'C

Cuts at:   1132    3176    1132 
   Size:       2044    3774
 
SspI AAT'ATT

Cuts at:      5      52    3742       5 
   Size:         47    3690    2081

  Fragments arranged by size: 

                3690    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2204    3011    1517 
   Size:        687     807    4324

  Fragments arranged by size: 

                4324     807     687
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2842    3049    3257     513 
   Size:        552     939     838     207     208    3074

  Fragments arranged by size: 

                3074     939     838     552     208     207
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2888    3011    3845    3862    4015    4200    4645 
   Size:       1255    1308     123     834      17     153     185     445

Cuts at:   4645     325 
   Size:       1498

  Fragments arranged by size: 

                1498    1308    1255     834     445     185     153     123
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2092    4064    5776 
   Size:        274      80      33      51    1443     115    1972    1712

Cuts at:   5776      96 
   Size:        138

  Fragments arranged by size: 

                1972    1712    1443     274     138     115      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5778    5810    5778 
   Size:         32    5786
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5818
 
XbaI T'CTAG_A

Cuts at:   3154    3154 
   Size:       5818
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2011    2524    2728    2011 
   Size:        513     204    5101

  Fragments arranged by size: 

                5101     513     204
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5818
 
XmaI C'CCGG_G

Cuts at:   1870    2822    3143    1870 
   Size:        952     321    4545

  Fragments arranged by size: 

                4545     952     321
 
XmnI GAAnn'nnTTC

Cuts at:   3947    3947 
   Size:       5818
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3626     276 
   Size:         53      83     186     936    2092    2468

  Fragments arranged by size: 

                2468    2092     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  Eco57MI    EcoNI    EcoRI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI     PstI     PvuI     SalI     SbfI     ScaI     SfcI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI  BstZ17I  EcoICRI EcoO109I    EcoRV
     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI
     NsiI     PacI    PpuMI   PspOMI    PspXI     PsrI    PvuII    RsrII
     SacI    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI
     SwaI
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